CrpaA.01374.a

Putative farnesyl pyrophosphate synthase

CENTER ID: CrpaA.01374.a
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.01374.a.A1.GE26527 Full length( CrpaA.01374.a ) 1 384
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd4_2550
OrthoMCL: OG5_127590
RefSeq: XP_625843.1
UniProt: Q5CR09
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
ILFHLLKITF IDSIFFALHD NYLTPQFIFN KMNDLQIEYD YTDFINYYDK FKVIVYNVLK KLPLNDEIRK PVIEYYLNCI DYNVKKGKHI RGKILVLISS LSSAYSNIKR DSIYLLGWVV EAIQALILIA DDIMDSGKFR RGAPCWYIVH GQSNAINDIF FLKMLSLSLI FELSSVFGND IVMKIQKIYN ESIFFTVLGQ HLDLSYFDLS KADKISERYF SMVEMKTSRY TFYMPVFFGL TLSEIQVSSA QLNLIEAILY KLGEFYQVHN DVSDYLFNDS NADDICRFKL TWPLQKSFEI ADEEMKLKIS ENYGKNSSLV KDCYNLLKIN EHYLEYQRNA LDYLIKLVKD ITDDSLQKVF IHLIHQISEL ITNSRSNADS NNSL
NT Sequence
ATTCTCTTTC ATCTATTAAA AATAACGTTT ATTGACTCAA TATTTTTTGC TCTGCACGAT AATTATTTAA CCCCTCAATT CATTTTCAAT AAAATGAATG ATTTGCAAAT AGAATATGAT TATACGGATT TTATTAATTA TTACGATAAA TTTAAAGTAA TAGTATATAA TGTTTTAAAA AAATTACCAT TAAATGATGA AATAAGAAAA CCAGTAATCG AATATTACTT AAACTGTATT GATTATAACG TAAAAAAAGG CAAGCATATT CGTGGCAAAA TTTTAGTGTT AATCTCAAGT TTAAGTTCAG CATATTCAAA TATAAAGAGA GACTCCATTT ATTTGTTAGG GTGGGTGGTT GAAGCAATAC AAGCATTGAT ATTAATTGCG GACGATATTA TGGATTCTGG TAAATTTAGA AGAGGAGCGC CTTGTTGGTA TATTGTTCAT GGTCAATCGA ATGCAATTAA TGACATTTTT TTCTTGAAAA TGCTATCACT TTCACTGATC TTTGAGCTTT CTAGTGTATT CGGAAATGAT ATTGTTATGA AAATCCAGAA AATATACAAT GAATCTATTT TTTTTACGGT ATTGGGCCAA CATTTAGATC TGTCATATTT TGATTTATCT AAAGCTGACA AAATATCCGA AAGATACTTT TCAATGGTAG AAATGAAGAC TTCTCGCTAT ACGTTTTATA TGCCTGTATT TTTTGGCTTA ACACTTTCTG AAATTCAAGT TTCTTCAGCG CAATTAAACT TGATTGAAGC TATCCTCTAT AAACTGGGCG AATTCTATCA AGTGCACAAT GATGTTTCTG ACTACTTATT TAATGATAGC AATGCAGATG ATATTTGCAG GTTTAAACTT ACATGGCCAC TACAAAAATC GTTTGAAATT GCTGATGAAG AAATGAAACT TAAGATTTCT GAAAACTATG GAAAAAATAG TTCGTTAGTA AAAGATTGTT ACAATTTATT AAAAATAAAT GAACATTATT TGGAATATCA AAGGAATGCA TTGGATTATT TAATTAAATT AGTGAAAGAT ATTACCGATG ATAGTCTACA GAAAGTATTT ATTCACCTAA TTCACCAAAT CTCGGAACTA ATTACAAATA GTCGATCAAA TGCAGATTCA AATAACTCAT TA
Details for CrpaA.01374.a.A1.GE26527
HARVESTED ON: 4/23/2009
SEQUENCED ON: 5/21/2009
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 92
Validated AA Sequence
MILFHLLKIT FIDSTFFALH DNYLTPQFIF NKMNDLQIEY DYTDFINYYD KFKVIVYNVL KKLPLNDEIR KPVIXYYLNC IDYNVKKGKH IRGKILVLIS SLSSAYSNIK RDSIYLLGWV VEAIQALILI ADDIMDSGKF RRGAPCWYIV HGQSNAINDI FFLKMLSLSL IFELSSVFGN DIVMKIQKIY NESIFFTVLG QHLDLSYFDL SKADKISERY FSMVEMKTSR YTFYMPVFFG LTLSEIQVSS AQLNLIEAIL YKLGEFYQVH NDVSDYLFND SNADDICRFK LTWPLQKSFE IADEEMKLKI SENYGKNSSL VKDCYNLLKI NEHYLEYQRN ALDYLIKLVK DITDDSLQKV FIHLIHQISE LITNSRS
Validated NT Sequence
atttgatcga ctatttgtaa ttagttccga gatttggtga attaggtgaa taaatacttt ctgtagacta tcatcggtaa tatctttcac taatttaatt aaataatcca atgcattcct ttgatattcc aaataatgtt catttatttt taataaattg taacaatctt ttactaacga actatttttt ccatagtttt cagaaatctt aagtttcatt tcttcatcag caatttcaaa cgatttttgt agtggccatg taagtttaaa cctgcaaata tcatctgcat tgctatcatt aaataagtag tcagaaacat cattgtgcac ttgatagaat tcgcccagtt tatagaggat agcttcaatc aagtttaatt gcgctgaaga aacttgaatt tcagaaagtg ttaagccaaa aaatacaggc atataaaacg tatagcgaga agtcttcatt tctaccattg aaaagtatct ttcggatatt ttgtcagctt tagataaatc aaaatatgac agatctaaat gttggcccaa taccgtaaaa aaaatagatt cattgtatat tttctggatt ttcataacaa tatcatttcc gaatacacta gaaagctcaa agatcagtga aagtgatagc attttcaaga aaaaaatgtc attaattgca ttcgattgac catgaacaat ataccaacaa ggcgctcctc ttctaaattt accagaatcc ataatatcgt ccgcaattaa tatcaatgct tgtattgctt caaccaccca ccctaacaaa taaatggagt ctctctttat atttgaatat gctgaactta aacttgagat taacactaaa attttgccac gaatatgctt gccttttttt acgttataat caatacagtt taagtaatat tngattactg gttttcttat ttcatcattt aatggtaatt tttttaaaac attatatact attactttaa atttatcgta ataattaata aaatccgtat aatcatattc tatttgcaaa tcattcattt tattgaaaat gaattgaggg gttaaataat tatcgtgcag agcaaaaaat gttgagtcaa taaacgttat ttttaataga tgaaagagaa tcatcgaacc aggaccctgg g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMILFHLLKI TFIDSIFFAL HDNYLTPQFI FNKMNDLQIE YDYTDFINYY DKFKVIVYNV LKKLPLNDEI RKPVIEYYLN CIDYNVKKGK HIRGKILVLI SSLSSAYSNI KRDSIYLLGW VVEAIQALIL IADDIMDSGK FRRGAPCWYI VHGQSNAIND IFFLKMLSLS LIFELSSVFG NDIVMKIQKI YNESIFFTVL GQHLDLSYFD LSKADKISER YFSMVEMKTS RYTFYMPVFF GLTLSEIQVS SAQLNLIEAI LYKLGEFYQV HNDVSDYLFN DSNADDICRF KLTWPLQKSF EIADEEMKLK ISENYGKNSS LVKDCYNLLK INEHYLEYQR NALDYLIKLV KDITDDSLQK VFIHLIHQIS ELITNSRSNA DSNNSL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattctcttt catctattaa aaataacgtt tattgactca atattttttg ctctgcacga taattattta acccctcaat tcattttcaa taaaatgaat gatttgcaaa tagaatatga ttatacggat tttattaatt attacgataa atttaaagta atagtatata atgttttaaa aaaattacca ttaaatgatg aaataagaaa accagtaatc gaatattact taaactgtat tgattataac gtaaaaaaag gcaagcatat tcgtggcaaa attttagtgt taatctcaag tttaagttca gcatattcaa atataaagag agactccatt tatttgttag ggtgggtggt tgaagcaata caagcattga tattaattgc ggacgatatt atggattctg gtaaatttag aagaggagcg ccttgttggt atattgttca tggtcaatcg aatgcaatta atgacatttt tttcttgaaa atgctatcac tttcactgat ctttgagctt tctagtgtat tcggaaatga tattgttatg aaaatccaga aaatatacaa tgaatctatt ttttttacgg tattgggcca acatttagat ctgtcatatt ttgatttatc taaagctgac aaaatatccg aaagatactt ttcaatggta gaaatgaaga cttctcgcta tacgttttat atgcctgtat tttttggctt aacactttct gaaattcaag tttcttcagc gcaattaaac ttgattgaag ctatcctcta taaactgggc gaattctatc aagtgcacaa tgatgtttct gactacttat ttaatgatag caatgcagat gatatttgca ggtttaaact tacatggcca ctacaaaaat cgtttgaaat tgctgatgaa gaaatgaaac ttaagatttc tgaaaactat ggaaaaaata gttcgttagt aaaagattgt tacaatttat taaaaataaa tgaacattat ttggaatatc aaaggaatgc attggattat ttaattaaat tagtgaaaga tattaccgat gatagtctac agaaagtatt tattcaccta attcaccaaa tctcggaact aattacaaat agtcgatcaa atgcagattc aaataactca ttaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa