CrpaA.01340.a

Thioredoxin reductase 1

CENTER ID: CrpaA.01340.a
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.01340.a.B2.GE35735 mature protein 27 526
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd2_4320
RefSeq: XP_626611.1
UniProt: Q5CT58

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
FFVRVMKFTL VTILFLIFMK KAKIVMADLT FMYDLVVIGG GSGGMAAAKE AAKYGKKVAL FDFVKPSTQG TKWGLGGTCV NVGCVPKKLM HYSALIASSI HHDAQMFGHK TSSSFEWGKL VETLRNHIRM LNFSYRTGLR VGNVEYINAL AKLIDPHSVE YEDNGQKKTI TSRYILLATG GRPSIPETVP GAIQYSITSD DIFFLSKSPG KTLVIGASYI GLETAGFLNE LGFDTTVAMR SIPLRGFDRQ CSEKIVEYMK ATGTKFLVGV VPINIEKVNE NIKVSFSDGS VEEFETVLYA TGRNPDVKGL NLNAIGVEVS DSGKIIAPKD ATSVPSIFAV GDIVEGRPEL TPVAVKAGIL LARRLFAGSN EFIDYDFVPT TVFTPIEYGH VGLSSEAAIA KYGEDDIEEY LSEFSTLEIA AAHREKPEHL RENEMDFALP LNCLAKLVVV KSQGEKVVGF HFVGPNAGEI TQGFSLAVKL GATKKDFDDM IGIHPTDAEV FGILEVTKRS GESFVSSGGC GGGKCG
NT Sequence
ttttttgttc gtgtaatgaa atttacgcta gtcacaatat tgtttttgat atttatgaaa aaggcgaaaa tagtcatggc tgatctaacg tttatgtatg atttagttgt aattggtggt ggttctggtg gaatggcagc tgcaaaagag gcagctaagt atggtaaaaa ggtggcacta ttcgattttg taaagccttc tactcaaggt acgaagtggg gactaggagg aacatgcgtg aatgtgggat gtgtaccaaa gaagcttatg cactactcag cattaatagc tagtagcatt caccatgatg ctcaaatgtt tggacacaaa actagttcta gcttcgaatg gggaaaatta gtagaaactt tacgaaatca tattagaatg cttaacttta gctaccgtac aggactacgt gtgggtaatg ttgaatacat caatgcactg gcaaagttaa ttgaccccca ttctgttgaa tatgaggaca atggccagaa aaaaacaatt acttctagat acatcttatt agcaacagga ggtcggccta gcatcccaga aaccgttcca ggagctatcc aatattcgat tacctctgat gatatcttct ttctctctaa aagccctggg aagactctag tcattggagc ttcttacatt ggattagaga cagctgggtt cctaaatgag ttaggcttcg atacaacagt tgcaatgaga tcaattccat tgagaggatt cgacagacaa tgttctgaga agattgttga atatatgaag gcaacgggaa ctaagttcct tgttggtgtt gtaccaatta atattgaaaa ggtaaatgag aatattaagg tgtctttcag cgatggctct gttgaagagt ttgaaactgt tttatatgca acaggtcgta acccagatgt aaagggactt aatctgaatg ctatcggagt tgaagtttcg gattctggta agattattgc ccccaaagat gctacttctg ttccaagtat atttgcagta ggagatattg ttgagggaag acccgaacta actcctgttg cagttaaggc tggaattctt ttagcaagaa ggctttttgc tgggtctaac gaatttattg attatgattt tgtccctact accgtattta cacctattga atatgggcat gttgggcttt catctgaagc tgcaattgct aaatatgggg aggatgatat tgaggagtat ctttccgaat ttagtacact tgaaattgct gctgctcata gagaaaaacc tgaacatctt cgcgaaaatg agatggactt tgcacttcct ttaaactgtt tggcgaagct tgttgtagtt aagagtcaag gtgaaaaagt tgttggattc cattttgttg gtcccaatgc tggagaaatt actcaagggt tctcgcttgc agtcaaactt ggggcaacta aaaaggactt tgatgatatg attggaattc atccaactga tgctgaagta tttggcattt tagaagttac caaaagatca ggagagtcat tcgtttcttc tggaggatgt ggtggcggaa agtgcggata a
Details for CrpaA.01340.a.B2.GE35735
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/6/2012
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMA DLTFMYDLXV IGGGSGGMAA AKEAAKYGKK VALFDFVKPS TQGTKWGLGG TCVNVGCVPK KLMXYSALIA SSIHHDAQMF GHKTSSSFXW GKLVETLRNH IXMLNFSYRT GLRVGNVEYI NALAKLIDPH SVEYEDNGQK KTXXSRYILL ATGGRPSIPE TVPGAIQYSI TSDDIFFLSK SPGKTLVIGA SYIGLETAGF LNELGFDTTV AMRSIPLRGF DRQCSEKXVE YMKATGTXFL VGVVPINIEK VNENIKVSFS DGSVEEXETV LXATGRNPDV KGLNLXAIGV EXSDSGKIIA PKDATSVPSI FAVGDIVEGR PELTPVAVKA GILLARRLFA GSNEFIDYDF VPTTVFTPIE YGHVGLSSEA AIAKYGEDDI EEYLSEFSTL EIAAAHREKP EHLRENEMDF ALPLNCLAKL VVVKSQGEKV VGFHFVGPNA GEITQGFSLA VKLGATKKDF DDMIGIHPTD AEVFGILEVT KRSGES
Validated NT Sequence
atggctcacc accaccacca ccatatggct gatctaacgt ttatgtatga tttanttgta attggtggtg gttctggtgg aatggcagct gcaaaagagg cagctaagta tggtaaaaag gtggcactat tcgattttgt aaagccttct actcaaggta cgaagtgggg actaggagga acatgcgtga atgtgggatg tgtaccaaag aagcttatgc nctactcagc attaatagct agtagcattc accatgatgc tcaaatgttt ggacacaaaa ctagttctag cttcnaatgg ggaaaattag tagaaacttt acgaaatcat attanaatgc ttaactttag ctaccgtaca ggactacgtg tgggtaatgt tgaatacatc aatgcactgg caaagttaat tgacccccat tctgttgaat atgaggacaa tggccagaaa aaaacannnn cntctagata catcttatta gcaacaggag gtcggcctag catcccagaa accgttccag gagctatcca atattcgatt acctctgatg atatcttctt tctctctaaa agccctggga agactctagt cattggagct tcttacattg gattagagac agctgggttc ctaaatgagt taggcttcga tacaacagtt gcnatgagat caattccntt gagaggattc gacagacaat gttctgagaa gantgttgaa tatatgaagg cnacgggaac tangttcctt gttggtgttg taccaattaa tattgaaaag gtaaatgaga atattaaggt gtctttcagc gatggctctg ttgaagagtt ngaaactgtt ttatnngcaa caggtcgtaa cccagatgta aagggactta atctgantgc tatcggagtn gaagnntcgg attctggtaa gattattgcc cccaaagatg ctacttctgt tccaagtata tttgcagtag gagatattgt tgagggaaga cccgaactaa ctcctgttgc agttaaggct ggaattcttt tagcaagaag gctttttgct gggtctaacg aatttattga ttatgatttt gtccctacta ccgtatttac acctattgaa tatgggcatg ttgggctttc atctgaagct gcaattgcta aatatgggga ggatgatatt gaggagtatc tttccgaatt tagtacactt gaaattgctg ctgctcatag agaaaaacct gaacatcttc gcgaaaatga gatggacttt gcacttcctt taaactgttt ggcgaagctt gttgtagtta agagtcaagg tgaaaaagtt gttggattcc attttgttgg tcccaatgct ggagaaatta ctcaagggtt ctcgcttgca gtcaaacttg gggcaactaa aaaggacttt gatgatatga ttggaattca tccaactgat gctgaagtat ttggcatttt agaagttacc aaaagatcag gagagtcatt c
Expected Protein Sequence
MAHHHHHHMA DLTFMYDLVV IGGGSGGMAA AKEAAKYGKK VALFDFVKPS TQGTKWGLGG TCVNVGCVPK KLMHYSALIA SSIHHDAQMF GHKTSSSFEW GKLVETLRNH IRMLNFSYRT GLRVGNVEYI NALAKLIDPH SVEYEDNGQK KTITSRYILL ATGGRPSIPE TVPGAIQYSI TSDDIFFLSK SPGKTLVIGA SYIGLETAGF LNELGFDTTV AMRSIPLRGF DRQCSEKIVE YMKATGTKFL VGVVPINIEK VNENIKVSFS DGSVEEFETV LYATGRNPDV KGLNLNAIGV EVSDSGKIIA PKDATSVPSI FAVGDIVEGR PELTPVAVKA GILLARRLFA GSNEFIDYDF VPTTVFTPIE YGHVGLSSEA AIAKYGEDDI EEYLSEFSTL EIAAAHREKP EHLRENEMDF ALPLNCLAKL VVVKSQGEKV VGFHFVGPNA GEITQGFSLA VKLGATKKDF DDMIGIHPTD AEVFGILEVT KRSGESFVSS GGCGGGKCG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg atctaacgtt tatgtatgat ttagttgtaa ttggtggtgg ttctggtgga atggcagctg caaaagaggc agctaagtat ggtaaaaagg tggcactatt cgattttgta aagccttcta ctcaaggtac gaagtgggga ctaggaggaa catgcgtgaa tgtgggatgt gtaccaaaga agcttatgca ctactcagca ttaatagcta gtagcattca ccatgatgct caaatgtttg gacacaaaac tagttctagc ttcgaatggg gaaaattagt agaaacttta cgaaatcata ttagaatgct taactttagc taccgtacag gactacgtgt gggtaatgtt gaatacatca atgcactggc aaagttaatt gacccccatt ctgttgaata tgaggacaat ggccagaaaa aaacaattac ttctagatac atcttattag caacaggagg tcggcctagc atcccagaaa ccgttccagg agctatccaa tattcgatta cctctgatga tatcttcttt ctctctaaaa gccctgggaa gactctagtc attggagctt cttacattgg attagagaca gctgggttcc taaatgagtt aggcttcgat acaacagttg caatgagatc aattccattg agaggattcg acagacaatg ttctgagaag attgttgaat atatgaaggc aacgggaact aagttccttg ttggtgttgt accaattaat attgaaaagg taaatgagaa tattaaggtg tctttcagcg atggctctgt tgaagagttt gaaactgttt tatatgcaac aggtcgtaac ccagatgtaa agggacttaa tctgaatgct atcggagttg aagtttcgga ttctggtaag attattgccc ccaaagatgc tacttctgtt ccaagtatat ttgcagtagg agatattgtt gagggaagac ccgaactaac tcctgttgca gttaaggctg gaattctttt agcaagaagg ctttttgctg ggtctaacga atttattgat tatgattttg tccctactac cgtatttaca cctattgaat atgggcatgt tgggctttca tctgaagctg caattgctaa atatggggag gatgatattg aggagtatct ttccgaattt agtacacttg aaattgctgc tgctcataga gaaaaacctg aacatcttcg cgaaaatgag atggactttg cacttccttt aaactgtttg gcgaagcttg ttgtagttaa gagtcaaggt gaaaaagttg ttggattcca ttttgttggt cccaatgctg gagaaattac tcaagggttc tcgcttgcag tcaaacttgg ggcaactaaa aaggactttg atgatatgat tggaattcat ccaactgatg ctgaagtatt tggcatttta gaagttacca aaagatcagg agagtcattc gtttcttctg gaggatgtgg tggcggaaag tgcggatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t