CrpaA.01271.a

Elongation factor 1-alpha

CENTER ID:	CrpaA.01271.a
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.01271.a.A1.GE26517	Full length( CrpaA.01271.a )		1	435

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd6_3990
OrthoMCL:	OG5_126631
RefSeq:	XP_001388344.1
UniProt:	A3FPW5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGKEKTHINL VVIGHVDSGK STTTGHLIYK LGGIDKRTIE KFEKESSEMG KGSFKYAWVL DKLKAERERG ITIDIALWQF ETPKYHYTVI DAPGHRDFIK NMITGTSQAD VALLVVPADR FEGAFSKEGQ TREHALLAFT LGVRQMIVGI NKMDTCEYKQ SRFDEIFNEV DGYLKKVGYN TEKIPFVAIS GFVGDNMVER SDKMPWYKGK TLVEALDTME PPKRPTDKPL RLPLQDVYKI GGVGTVPVGR VETGIIRPGM NVTFAPAGVT TEVKSVEMHH EQMPEAVPGD NVGFNVKNVS IKDIKRGFVA SDAKNDPAKG CEDFTAQVIV LNHPGEIKNG YSPVVDCHTA HISCKFQTIT AKMDKRSGKV LEENPKLIKS GDAALVVMQP LKPLCVEAFT DYPPLGRFAV RDMKQTVAVG VIKSVTKKEA TSKKK

NT Sequence

ATGGGTAAGG AAAAGACTCA CATTAACTTG GTCGTTATTG GTCACGTCGA CTCTGGTAAA TCAACCACCA CTGGTCACTT GATTTACAAG CTCGGTGGTA TTGATAAGAG AACTATCGAA AAGTTCGAGA AGGAATCTTC CGAAATGGGT AAGGGTTCCT TCAAATACGC CTGGGTTTTG GATAAGCTCA AGGCTGAGAG AGAAAGAGGT ATTACCATCG ATATTGCTTT ATGGCAATTC GAAACCCCAA AATACCACTA CACTGTCATT GATGCCCCAG GTCACAGAGA TTTCATCAAG AATATGATTA CTGGTACCTC TCAAGCTGAT GTTGCTTTAT TGGTTGTCCC AGCCGATCGT TTCGAAGGTG CCTTCTCCAA GGAAGGTCAA ACCAGAGAAC ATGCTTTATT GGCCTTCACT TTGGGTGTCA GACAAATGAT TGTCGGTATT AACAAGATGG ATACCTGTGA ATACAAGCAA TCTCGTTTTG ATGAAATCTT CAACGAAGTT GATGGTTACC TCAAGAAGGT TGGTTACAAC ACCGAGAAGA TCCCATTCGT TGCCATTTCT GGTTTCGTTG GTGATAATAT GGTTGAGAGA TCTGACAAGA TGCCATGGTA TAAGGGTAAG ACCTTAGTCG AAGCCCTCGA CACTATGGAA CCACCAAAGA GACCAACTGA CAAGCCACTC CGTCTCCCAT TACAAGATGT TTACAAGATA GGTGGTGTAG GTACTGTCCC AGTCGGTCGT GTTGAGACTG GTATCATCAG ACCAGGTATG AATGTTACCT TCGCTCCAGC TGGTGTTACC ACTGAAGTTA AGTCAGTAGA AATGCACCAT GAGCAGATGC CAGAGGCCGT CCCAGGTGAC AACGTTGGTT TCAATGTTAA GAACGTCTCC ATCAAGGATA TCAAGAGAGG TTTCGTTGCT TCTGATGCCA AGAATGACCC AGCTAAGGGC TGTGAAGACT TCACTGCTCA AGTTATCGTC CTCAACCACC CAGGTGAAAT CAAGAACGGT TACTCTCCAG TCGTTGACTG TCACACCGCT CACATTTCCT GCAAATTCCA GACTATCACT GCTAAGATGG ACAAGAGATC TGGTAAGGTT TTGGAAGAAA ACCCAAAGCT TATCAAGTCT GGTGATGCTG CTTTGGTTGT TATGCAACCT TTGAAGCCAC TTTGTGTTGA GGCCTTCACT GACTACCCAC CTCTAGGTCG TTTCGCTGTC CGTGATATGA AGCAAACCGT TGCTGTCGGT GTTATTAAGT CTGTTACCAA GAAAGAAGCC ACTTCCAAGA AGAAG

Details for CrpaA.01271.a.A1.GE26517

HARVESTED ON:	4/20/2009
SEQUENCED ON:	5/8/2009
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGKEKTHIN LVVIGHVDSG KSTTTGHLIY KLGGIDKRTI EKFEKESSEM GKGSFKYAWV LDKLKAERER GITIDIALWQ FETPKYHYTV IDAPGHRDFI KNMITGTSQA DVALLVVPAD RFEGAFSKEG QTREHALLAF TLGVRQMIVG INKMDTCEYK QSRFDEIFNE VDGYLKKVGY NTEKIPFVAI SGFVGDNMVE RSDKMPWYKG KTLVEALDTM EPPKRPTDKP LRLPLQDVYK IGGVGTVPVG RVETGIIRPG MNVTFAPAGV TTEVKSVEMH HEQMPEAVPG DNVGFNVKNV SIKDIKRGFV ASDAKNDPAK GCEDFTAQVI VLNHPGEIKN GYSPVVDCHT AHISCKFQTI TAKMDKRSGK VLEENPKLIK SGDAALVVMQ PLKPLCVEAF TDYPPXGRFA VRDMKQTVAV GVIKSVTKKE ATSK

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggta aggaaaagac tcacattaac ttggtcgtta ttggtcacgt cgactctggt aaatcaacca ccactggtca cttgatttac aagctcggtg gtattgataa gagaactatc gaaaagttcg agaaggaatc ttccgaaatg ggtaagggtt ccttcaaata cgcctgggtt ttggataagc tcaaggctga gagagaaaga ggtattacca tcgatattgc tttatggcaa ttcgaaaccc caaaatacca ctacactgtc attgatgccc caggtcacag agatttcatc aagaatatga ttactggtac ctctcaagct gatgttgctt tattggttgt cccagccgat cgtttcgaag gtgccttctc caaggaaggt caaaccagag aacatgcttt attggccttc actttgggtg tcagacaaat gattgtcggt attaacaaga tggatacctg tgaatacaag caatctcgtt ttgatgaaat cttcaacgaa gttgatggtt acctcaagaa ggttggttac aacaccgaga agatcccatt cgttgccatt tctggtttcg ttggtgataa tatggttgag agatctgaca agatgccatg gtataagggt aagaccttag tcgaagccct cgacactatg gaaccaccaa agagaccaac tgacaagcca ctccgtctcc cattacaaga tgtttacaag ataggtggtg taggtactgt cccagtcggt cgtgttgaga ctggtatcat cagaccaggt atgaatgtta ccttcgctcc agctggtgtt accactgaag ttaagtcagt agaaatgcac catgagcaga tgccagaggc cgtcccaggt gacaacgttg gtttcaatgt taagaacgtc tccatcaagg atatcaagag aggtttcgtt gcttctgatg ccaagaatga cccagctaag ggctgtgaag acttcactgc tcaagttatc gtcctcaacc acccaggtga aatcaagaac ggttactctc cagtcgttga ctgtcacacc gctcacattt cctgcaaatt ccagactatc actgctaaga tggacaagag atctggtaag gttttggaag aaaacccaaa gcttatcaag tctggtgatg ctgctttggt tgttatgcaa cctttgaagc cactttgtgt tgaggccttc actgactacc cacctntagg tcgtttcgct gtccgtgata tgaagcaaac cgttgctgtc ggtgttatta agtctgttac caagaaagaa gccacttcca aga

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtaaggaa aagactcaca ttaacttggt cgttattggt cacgtcgact ctggtaaatc aaccaccact ggtcacttga tttacaagct cggtggtatt gataagagaa ctatcgaaaa gttcgagaag gaatcttccg aaatgggtaa gggttccttc aaatacgcct gggttttgga taagctcaag gctgagagag aaagaggtat taccatcgat attgctttat ggcaattcga aaccccaaaa taccactaca ctgtcattga tgccccaggt cacagagatt tcatcaagaa tatgattact ggtacctctc aagctgatgt tgctttattg gttgtcccag ccgatcgttt cgaaggtgcc ttctccaagg aaggtcaaac cagagaacat gctttattgg ccttcacttt gggtgtcaga caaatgattg tcggtattaa caagatggat acctgtgaat acaagcaatc tcgttttgat gaaatcttca acgaagttga tggttacctc aagaaggttg gttacaacac cgagaagatc ccattcgttg ccatttctgg tttcgttggt gataatatgg ttgagagatc tgacaagatg ccatggtata agggtaagac cttagtcgaa gccctcgaca ctatggaacc accaaagaga ccaactgaca agccactccg tctcccatta caagatgttt acaagatagg tggtgtaggt actgtcccag tcggtcgtgt tgagactggt atcatcagac caggtatgaa tgttaccttc gctccagctg gtgttaccac tgaagttaag tcagtagaaa tgcaccatga gcagatgcca gaggccgtcc caggtgacaa cgttggtttc aatgttaaga acgtctccat caaggatatc aagagaggtt tcgttgcttc tgatgccaag aatgacccag ctaagggctg tgaagacttc actgctcaag ttatcgtcct caaccaccca ggtgaaatca agaacggtta ctctccagtc gttgactgtc acaccgctca catttcctgc aaattccaga ctatcactgc taagatggac aagagatctg gtaaggtttt ggaagaaaac ccaaagctta tcaagtctgg tgatgctgct ttggttgtta tgcaaccttt gaagccactt tgtgttgagg ccttcactga ctacccacct ctaggtcgtt tcgctgtccg tgatatgaag caaaccgttg ctgtcggtgt tattaagtct gttaccaaga aagaagccac ttccaagaag aagaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa