CrpaA.01255.a

Disulfide-isomerase, signal peptide plus ER retention motif, putative ER protein

CENTER ID:	CrpaA.01255.a
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.01255.a.A1.GE26469	Full length( CrpaA.01255.a )		1	481

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd6_120
OrthoMCL:	OG5_126843
RefSeq:	XP_625352.1
UniProt:	Q5CPJ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIGIRSLVSA AFLGFSCLSK VVLGGDEAHF ISEHITSLTS SNFEDFIKSK EHVIVTFFAP WCGHCTALEP EFKATCAEIS KLSPPVHCGS VDATENMELA QQYGVSGYPT IKFFSGIDSV QNYSGARSKD AFIKYIKKLT GPAVQVAESE EAIKTIFASS SSAFVGRFTS KDSAEYAVFE KVASGHREHN YAFIAFFQEG EQKLEVLHKD EEPVSLPMPK TVEELEAKIS IMNVPLFSAI SAENYSLYMS REGYTAWFCG TNEDFAKYAS NIRKVAADYR EKYAFVFLDT EQFGSHATQH LLIEKFPGLV IQSVNVPSIR YMYGPAKFDS VEPLKEFMKQ VSEGKHELSI KSEPIPAEQS GPVTVVVGKT FEEIVFRSDK DVLLEIYAQW CGHCKNLEPI YNQLGEEYKD NDKVVIAKIN GPQNDIPYEG FSPRAFPTIL FVKAGTRTPI PYDGKRTVEA FKEFISEHSS FPQEKESRDE L

NT Sequence

ATGATCGGAA TTAGAAGCTT GGTTTCAGCA GCATTTTTAG GTTTTTCTTG TCTCTCCAAG GTAGTCTTGG GTGGAGATGA AGCTCACTTC ATTTCAGAAC ACATTACTTC CTTAACTTCC TCCAACTTCG AAGACTTCAT TAAGAGCAAG GAACACGTAA TTGTTACTTT CTTTGCCCCA TGGTGCGGCC ATTGTACTGC TTTAGAGCCA GAATTCAAGG CAACATGCGC TGAAATCTCA AAGCTCTCTC CCCCAGTACA CTGTGGCAGT GTTGATGCAA CTGAAAATAT GGAGCTTGCA CAACAATATG GTGTGAGCGG ATACCCAACC ATCAAATTCT TCAGTGGTAT TGACAGTGTT CAGAACTATT CAGGAGCAAG AAGCAAGGAT GCATTCATCA AGTATATTAA GAAGTTGACC GGACCAGCAG TCCAAGTTGC TGAATCAGAA GAAGCTATCA AGACAATCTT CGCTTCTTCT TCTTCAGCCT TTGTTGGAAG ATTCACCTCT AAGGACTCAG CTGAGTATGC TGTCTTCGAG AAGGTTGCTA GTGGTCACCG CGAGCACAAC TATGCTTTCA TTGCTTTCTT CCAAGAAGGT GAACAAAAGC TCGAGGTATT ACACAAGGAC GAGGAGCCAG TTTCTCTCCC AATGCCAAAG ACTGTTGAAG AGTTGGAGGC CAAGATATCC ATAATGAATG TACCATTGTT CTCTGCAATT AGTGCTGAGA ACTACTCCCT CTATATGTCA AGAGAAGGTT ATACTGCCTG GTTCTGTGGT ACTAACGAGG ACTTCGCCAA GTATGCTTCA AACATTAGAA AGGTTGCAGC TGATTACAGA GAAAAGTATG CCTTTGTTTT CCTTGATACT GAGCAATTTG GTTCCCATGC TACTCAACAT CTCTTAATTG AGAAATTCCC AGGTTTGGTT ATCCAAAGTG TCAATGTTCC ATCAATTAGA TACATGTATG GTCCAGCTAA ATTCGACTCT GTTGAGCCAT TAAAGGAATT TATGAAGCAA GTTTCTGAAG GCAAGCACGA ACTCAGCATT AAGTCTGAGC CAATCCCAGC TGAGCAATCT GGTCCAGTCA CTGTTGTTGT TGGTAAGACC TTCGAAGAAA TTGTTTTCAG AAGTGACAAG GATGTTCTTT TGGAAATCTA TGCCCAATGG TGTGGACACT GTAAGAACCT CGAGCCAATC TACAACCAAC TCGGCGAAGA GTACAAGGAC AACGACAAGG TTGTGATTGC AAAGATCAAT GGACCACAAA ACGATATCCC ATATGAAGGT TTCAGTCCAA GAGCCTTCCC AACTATCTTG TTCGTCAAGG CCGGAACTAG AACCCCAATT CCTTATGATG GAAAGAGAAC TGTTGAGGCC TTCAAGGAAT TCATCAGTGA ACATTCTTCC TTCCCTCAAG AAAAGGAATC TCGTGACGAA CTC

Details for CrpaA.01255.a.A1.GE26469

HARVESTED ON:	4/20/2009
SEQUENCED ON:	3/21/2012
EXPECTED MW:	56kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	71
PERCENT COVERAGE:	86

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMIGIRSLVS AAFLGFSCLS KVVLGGDEAH FISEHITSLT SSNFEDFIKS KEHVIVTFFA PWCGHCTALE PEFKATCAEI SKLSPPVHCG SVDATENMEL AQQYGVSGYP TIKFFSGIDS VQNYSGARSK DAFIKYIKKL TGPAVQVAES EEAIKTIFAS SSSAFVGRFT SKDSAEYAVF EKVASXHREH NYAFIAFFQE GEQKLEVLHK DEXPVSLPMP KTVEELEAKI SIMNVPLXSA ISAXNYSLYM SRXXXTXWXC GTNEDFAKYA SNIRKVAADY REXMPLFSXI LSXXVPXXLX XSXXRNSQVW LXKVSMFHQX DTXMVQXXXX LXXHXXNLXX KFXKASTNSA LSXXQSQXSN LXQSXLLLVR PSKKLFSXVT RMFFWKSMPX GVDTVRTSSQ XTTNSAKSTR TTTRL

Validated NT Sequence

gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatcg gaattagaag cttggtttca gcagcatttt taggtttttc ttgtctctcc aaggtagtct tgggtggaga tgaagctcac ttcatttcag aacacattac ttccttaact tcctccaact tcgaagactt cattaagagc aaggaacacg taattgttac tttctttgcc ccatggtgcg gccattgtac tgctttagag ccagaattca aggcaacatg cgctgaaatc tcaaagctct ctcccccagt acactgtggc agtgttgatg caactgaaaa tatggagctt gcacaacaat atggtgtgag cggataccca accatcaaat tcttcagtgg tattgacagt gttcagaact attcaggagc aagaagcaag gatgcattca tcaagtatat taagaagttg accggaccag cagtccaagt tgctgaatca gaagaagcta tcaagacaat cttcgcttct tcttcttcag cctttgttgg aagattcacc tctaaggact cagctgagta tgctgtcttc gagaaggttg ctagtgntca ccgcgagcac aactatgctt tcattgcttt cttccaagaa ggtgaacaaa agctcgaggt attacacaag gacgagganc cagtttctct cccaatgcca aagactgttg aagagttgga ggccaagata tccataatga atgtaccatt gntctctgca attagtgctg anaactactc cctctatatg tcaagagnnn nnnntactgn ctggntctgt ggtactaacg aggacttcgc naagtatgct tcaaacatta gaaaggttgc agctgattac agagaangta tgcctttgtt ttcnnnnata ctgagcannn nngttcccnn gntactcann ntntcnnaan tgagaaattc ccaggtttgg ttanccaaag tgtcaatgtt ccatcaatnn gatacangta tggtccagnn aaannnnnnt ctgntgannc attnaangaa tttatnnngc aagtttnnga aggcaagcac gaactcagca ttaagtnnga nncaatccca gntgagcaat ctggnccagt canngttgtt gttggtaaga ccttcgaaga aattgttttc agnagtgaca aggatgttct tttggaaatc tatgcccaan ggtgtggaca ctgtaagaac ctcgagccaa nctacaacca actcggcgaa gagtacaagg acaacgacaa ggttgtgatt gcaaagatca atggaccaca aaacgatatc ccatatgaag gtttcagtcc aagagccttc ccaactatct tgttcgtcaa ggccggaact agaaccccaa ttccttatga tggaaagaga actgttgagg ccttcaagga attcatcagt gaacattctt ccttccctca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIGIRSLVS AAFLGFSCLS KVVLGGDEAH FISEHITSLT SSNFEDFIKS KEHVIVTFFA PWCGHCTALE PEFKATCAEI SKLSPPVHCG SVDATENMEL AQQYGVSGYP TIKFFSGIDS VQNYSGARSK DAFIKYIKKL TGPAVQVAES EEAIKTIFAS SSSAFVGRFT SKDSAEYAVF EKVASGHREH NYAFIAFFQE GEQKLEVLHK DEEPVSLPMP KTVEELEAKI SIMNVPLFSA ISAENYSLYM SREGYTAWFC GTNEDFAKYA SNIRKVAADY REKYAFVFLD TEQFGSHATQ HLLIEKFPGL VIQSVNVPSI RYMYGPAKFD SVEPLKEFMK QVSEGKHELS IKSEPIPAEQ SGPVTVVVGK TFEEIVFRSD KDVLLEIYAQ WCGHCKNLEP IYNQLGEEYK DNDKVVIAKI NGPQNDIPYE GFSPRAFPTI LFVKAGTRTP IPYDGKRTVE AFKEFISEHS SFPQEKESRD EL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcggaatt agaagcttgg tttcagcagc atttttaggt ttttcttgtc tctccaaggt agtcttgggt ggagatgaag ctcacttcat ttcagaacac attacttcct taacttcctc caacttcgaa gacttcatta agagcaagga acacgtaatt gttactttct ttgccccatg gtgcggccat tgtactgctt tagagccaga attcaaggca acatgcgctg aaatctcaaa gctctctccc ccagtacact gtggcagtgt tgatgcaact gaaaatatgg agcttgcaca acaatatggt gtgagcggat acccaaccat caaattcttc agtggtattg acagtgttca gaactattca ggagcaagaa gcaaggatgc attcatcaag tatattaaga agttgaccgg accagcagtc caagttgctg aatcagaaga agctatcaag acaatcttcg cttcttcttc ttcagccttt gttggaagat tcacctctaa ggactcagct gagtatgctg tcttcgagaa ggttgctagt ggtcaccgcg agcacaacta tgctttcatt gctttcttcc aagaaggtga acaaaagctc gaggtattac acaaggacga ggagccagtt tctctcccaa tgccaaagac tgttgaagag ttggaggcca agatatccat aatgaatgta ccattgttct ctgcaattag tgctgagaac tactccctct atatgtcaag agaaggttat actgcctggt tctgtggtac taacgaggac ttcgccaagt atgcttcaaa cattagaaag gttgcagctg attacagaga aaagtatgcc tttgttttcc ttgatactga gcaatttggt tcccatgcta ctcaacatct cttaattgag aaattcccag gtttggttat ccaaagtgtc aatgttccat caattagata catgtatggt ccagctaaat tcgactctgt tgagccatta aaggaattta tgaagcaagt ttctgaaggc aagcacgaac tcagcattaa gtctgagcca atcccagctg agcaatctgg tccagtcact gttgttgttg gtaagacctt cgaagaaatt gttttcagaa gtgacaagga tgttcttttg gaaatctatg cccaatggtg tggacactgt aagaacctcg agccaatcta caaccaactc ggcgaagagt acaaggacaa cgacaaggtt gtgattgcaa agatcaatgg accacaaaac gatatcccat atgaaggttt cagtccaaga gccttcccaa ctatcttgtt cgtcaaggcc ggaactagaa ccccaattcc ttatgatgga aagagaactg ttgaggcctt caaggaattc atcagtgaac attcttcctt ccctcaagaa aaggaatctc gtgacgaact caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa