CrpaA.01238.a
Seryl-tRNA synthetase, cytoplasmic (SerRS)
| CENTER ID: | CrpaA.01238.a |
| ORGANISM: | Cryptosporidium parvum Iowa II |
| ASSOCIATED DISEASE: | cryptosporidiosis - (diarrheal disease) |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrpaA.01238.a.AE1.GE42988 | full length | 1 | 454 | ||
| CrpaA.01238.a.B1.GE41424 | full length | 1 | 454 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrpaA.01238.a.HW1.PW38389 | full length | 1 | 454 | ||
| CrpaA.01238.a.AE1.PW38630 | full length | 1 | 454 |
Structures
6OTE
DEPOSITED: 5/3/2019
DETERMINATION: XRay
CLONE: CrpaA.01238.a.HZ1.GE41770
PROTEIN: CrpaA.01238.a.HZ1.PW38382
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | CryptoDB:cgd8_4720 |
| UniProt: | Q5CVB3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPIDINRIRV EKGGDYQKIA ESEMARYKGL ETLEELVKVD QKWREDMFKL EQSKKELNSI SKEIAQIKKK DPKADCKDLQ DKSVQLKKNL PIIEKQALET EEVRDKLWHK IGNVLQPDVP ISNTEDDNLV LRTWGEIPDI KVDGTPGKLH HNEIMSRLGF YDSVKGAELA GHRGYFLKDY GVIMSMALSH YAMGFLLKKG YLAIQPPYFM KRDLMGKAAE LQDFEETLYH IPSDNSKGEV DSNSLFLIAT SEQPIAAMHH NVTLEDKDLP IKYAGISTCF RKEAGAHGKD TWGIFRIHQF EKVEQFCVTL PEDSQKIHEE MISISEEFYQ SLELPYRVIS IVSGALNDAA SKKYDLEAWF PGYNSYRELV SCSNCTDYQS RALECRLGFR KEGEREKHYC HFLNGTLCAI QRTMCCIVEN YQTPDGLRIP KVLQPYMNGV EFIPFKNETA TCAN
NT Sequence
atgcctatag atattaatag aattagagtg gagaaaggtg gagattatca aaagatagcc gagtcagaaa tggcaaggta taaaggacta gaaactcttg aggaactcgt taaagtagat cagaaatgga gagaggatat gtttaaatta gagcaaagca aaaaggagtt aaattcaatt tcaaaagaaa ttgctcaaat taaaaagaaa gatccaaagg cagattgcaa ggatttacaa gacaaaagcg ttcaattaaa gaagaatctt ccaattatcg agaaacaagc attggaaact gaagaagtaa gagataagtt atggcataaa attggtaatg tcttacaacc tgatgttcct atttccaata cagaggatga caaccttgta ttgagaacat ggggagaaat accagatatt aaggttgatg gaacaccagg gaaattgcat cacaatgaaa tcatgtccag attaggattc tatgatagtg tgaaaggtgc agaattagca ggccatcgtg gttacttttt aaaggattat ggtgtaataa tgagtatggc tctctcacat tatgctatgg gattcttgtt aaaaaaagga tatttggcaa ttcaaccgcc atactttatg aaaagagatt tgatgggtaa agcagctgaa ttgcaggact ttgaggagac actttatcac attccttctg ataacagcaa aggcgaggtt gattcaaata gcttattttt gattgcaaca tctgagcaac caatagctgc aatgcatcat aatgtgacac ttgaagataa ggacttacca atcaaatatg ctggtatttc aacatgcttt agaaaagagg caggcgctca tggtaaagat acctggggaa tatttagaat tcatcaattt gaaaaagttg agcaattctg tgttacttta ccagaagact cacaaaagat tcatgaggaa atgattagca tttccgagga attttatcaa tctttggaat taccttatag agtaatttca atcgtatcag gagcgcttaa tgacgctgca tcgaaaaagt atgatttaga agcttggttc cccggatata actcatatag ggaacttgtt tcatgctcaa actgtactga ttaccaatct agagcgcttg aatgtcgtct gggattcaga aaagaaggag agcgtgaaaa acattactgc catttcctaa acggtacttt atgtgcaatc caaagaacaa tgtgctgtat tgttgaaaac tatcaaactc cagatggtct tagaatacca aaagtcttac agccttatat gaatggcgtc gaattcattc cattcaaaaa cgaaaccgct acttgtgcca attag
Details for CrpaA.01238.a.AE1.GE42988
| HARVESTED ON: | 3/4/2019 |
| SEQUENCED ON: | 3/12/2019 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 53kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MPIDINRIRV EKGGDYQKIA ESEMARYKGL ETLEELVKVD QKWREDMFKL EQSKKELNSI SKEIAQIKKK DPKADCKDLQ DKSVQLKKNL PIIEKQALET EEVRDKLWHK IGNVLQPDVP ISNTEDDNLV LRTWGEIPDI KVDGTPGKLH HNEIMSRLGF YDSVKGAELA GHRGYFLKDY GVIMSMALSH YAMGFLLKKG YLAIQPPYFM KRDLMGKAAE LQDFEETLYH IPSDNSKGEV DSNSLFLIAT SEQPIAAMHH NVTLEDKDLP IKYAGISTCF RKEAGAHGKD TWGIFRIHQF EKVEQFCVTL PEDSQKIHEE MISISEEFYQ SLELPYRVIS IVSGALNDAA SKKYDLEAWF PGYNSYRELV SCSNCTDYQS RALECRLGFR KEGEREKHYC HFLNGTLCAI QRTMCCIVEN YQTPDGLRIP KVLQPYMNGV EFIPFKNETA TCANGHHHHH H
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gcctatagat attaatagaa ttagagtgga gaaaggtgga gattatcaaa agatagccga gtcagaaatg gcaaggtata aaggactaga aactcttgag gaactcgtta aagtagatca gaaatggaga gaggatatgt ttaaattaga gcaaagcaaa aaggagttaa attcaatttc aaaagaaatt gctcaaatta aaaagaaaga tccaaaggca gattgcaagg atttacaaga caaaagcgtt caattaaaga agaatcttcc aattatcgag aaacaagcat tggaaactga agaagtaaga gataagttat ggcataaaat tggtaatgtc ttacaacctg atgttcctat ttccaataca gaggatgaca accttgtatt gagaacatgg ggagaaatac cagatattaa ggttgatgga acaccaggga aattgcatca caatgaaatc atgtccagat taggattcta tgatagtgtg aaaggtgcag aattagcagg ccatcgtggt tactttttaa aggattatgg tgtaataatg agtatggctc tctcacatta tgctatggga ttcttgttaa aaaaaggata tttggcaatt caaccgccat actttatgaa aagagatttg atgggtaaag cagctgaatt gcaggacttt gaggagacac tttatcacat tccttctgat aacagcaaag gcgaggttga ttcaaatagc ttatttttga ttgcaacatc tgagcaacca atagctgcaa tgcatcataa tgtgacactt gaagataagg acttaccaat caaatatgct ggtatttcaa catgctttag aaaagaggca ggcgctcatg gtaaagatac ctggggaata tttagaattc atcaatttga aaaagttgag caattctgtg ttactttacc agaagactca caaaagattc atgaggaaat gattagcatt tccgaggaat tttatcaatc tttggaatta ccttatagag taatttcaat cgtatcagga gcgcttaatg acgctgcatc gaaaaagtat gatttagaag cttggttccc cggatataac tcatataggg aacttgtttc atgctcaaac tgtactgatt accaatctag agcgcttgaa tgtcgtctgg gattcagaaa agaaggagag cgtgaaaaac attactgcca tttcctaaac ggtactttat gtgcaatcca aagaacaatg tgctgtattg ttgaaaacta tcaaactcca gatggtctta gaataccaaa agtcttacag ccttatatga atggcgtcga attcattcca ttcaaaaacg aaaccgctac ttgtgccaat gggcaccacc atcatcatca ttaacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat
Details for CrpaA.01238.a.B1.GE41424
| HARVESTED ON: | 4/5/2017 |
| SEQUENCED ON: | 4/27/2017 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 55kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMP IDINRIRVEK GGDYQKIAES EMARYKGLET LEELVKVDQK WREDMFKLEQ SKKELNSISK EIAQIKKKDP KADCKDLQDK SVQLKKNLPI IEKQALETEE VRDKLWHKIG NVLQPDVPIS NTEDDNLVLR TWGEIPDIKV DGTPGKLHHN EIMSRLGFYD SVKGAELAGH RGYFLKDYGV IMSMALSHYA MGFLLKKGYL AIQPPYFMKR DLMGKAAELQ DFEETLYHIP SDNSKGEVDS NSLFLIATSE QPIAAMHHNV TLEDKDLPIK YAGISTCFRK EAGAHGKDTW GIFRIHQFEK VEQFCVTLPE DSQKIHEEMI SISEEFYQSL ELPYRVISIV SGALNDAASK KYDLEAWFPG YNSYRELVSC SNCTDYQSRA LECRLGFRKE GEREKHYCHF LNGTLCAIQR TMCCIVENYQ TPDGLRIPKV LQXYMNGVEF IPFK
Validated NT Sequence
atggctcacc accaccacca ccatatgcct atagatatta atagaattag agtggagaaa ggtggagatt atcaaaagat agccgagtca gaaatggcaa ggtataaagg actagaaact cttgaggaac tcgttaaagt agatcagaaa tggagagagg atatgtttaa attagagcaa agcaaaaagg agttaaattc aatttcaaaa gaaattgctc aaattaaaaa gaaagatcca aaggcagatt gcaaggattt acaagacaaa agcgttcaat taaagaagaa tcttccaatt atcgagaaac aagcattgga aactgaagaa gtaagagata agttatggca taaaattggt aatgtcttac aacctgatgt tcctatttcc aatacagagg atgacaacct tgtattgaga acatggggag aaataccaga tattaaggtt gatggaacac cagggaaatt gcatcacaat gaaatcatgt ccagattagg attctatgat agtgtgaaag gtgcagaatt agcaggccat cgtggttact ttttaaagga ttatggtgta ataatgagta tggctctctc acattatgct atgggattct tgttaaaaaa aggatatttg gcaattcaac cgccatactt tatgaaaaga gatttgatgg gtaaagcagc tgaattgcag gactttgagg agacacttta tcacattcct tctgataaca gcaaaggcga ggttgattca aatagcttat ttttgattgc aacatctgag caaccaatag ctgcaatgca tcataatgtg acacttgaag ataaggactt accaatcaaa tatgctggta tttcaacatg ctttagaaaa gaggcaggcg ctcatggtaa agatacctgg ggaatattta gaattcatca atttgaaaaa gttgagcaat tctgtgttac tttaccagaa gactcacaaa agattcatga ggaaatgatt agcatttccg aggaatttta tcaatctttg gaattacctt atagagtaat ttcaatcgta tcaggagcgc ttaatgacgc tgcatcgaaa aagtatgatt tagaagcttg gttccccgga tataactcat atagggaact tgtttcatgc tcaaactgta ctgattacca atctagagcg cttgaatgtc gtctgggatt cagaaaagaa ggagagcgtg aaaaacatta ctgccatttc ctaaacggta ctttatgtgc aatccaaaga acaatgtgct gtattgttga aaactatcaa actccagatg gtcttagaat accaaaagtc ttacagnnnt atatgaatgg cgtcgaattc attccattca aaa
Expected Protein Sequence
MAHHHHHHMP IDINRIRVEK GGDYQKIAES EMARYKGLET LEELVKVDQK WREDMFKLEQ SKKELNSISK EIAQIKKKDP KADCKDLQDK SVQLKKNLPI IEKQALETEE VRDKLWHKIG NVLQPDVPIS NTEDDNLVLR TWGEIPDIKV DGTPGKLHHN EIMSRLGFYD SVKGAELAGH RGYFLKDYGV IMSMALSHYA MGFLLKKGYL AIQPPYFMKR DLMGKAAELQ DFEETLYHIP SDNSKGEVDS NSLFLIATSE QPIAAMHHNV TLEDKDLPIK YAGISTCFRK EAGAHGKDTW GIFRIHQFEK VEQFCVTLPE DSQKIHEEMI SISEEFYQSL ELPYRVISIV SGALNDAASK KYDLEAWFPG YNSYRELVSC SNCTDYQSRA LECRLGFRKE GEREKHYCHF LNGTLCAIQR TMCCIVENYQ TPDGLRIPKV LQPYMNGVEF IPFKNETATC AN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccta tagatattaa tagaattaga gtggagaaag gtggagatta tcaaaagata gccgagtcag aaatggcaag gtataaagga ctagaaactc ttgaggaact cgttaaagta gatcagaaat ggagagagga tatgtttaaa ttagagcaaa gcaaaaagga gttaaattca atttcaaaag aaattgctca aattaaaaag aaagatccaa aggcagattg caaggattta caagacaaaa gcgttcaatt aaagaagaat cttccaatta tcgagaaaca agcattggaa actgaagaag taagagataa gttatggcat aaaattggta atgtcttaca acctgatgtt cctatttcca atacagagga tgacaacctt gtattgagaa catggggaga aataccagat attaaggttg atggaacacc agggaaattg catcacaatg aaatcatgtc cagattagga ttctatgata gtgtgaaagg tgcagaatta gcaggccatc gtggttactt tttaaaggat tatggtgtaa taatgagtat ggctctctca cattatgcta tgggattctt gttaaaaaaa ggatatttgg caattcaacc gccatacttt atgaaaagag atttgatggg taaagcagct gaattgcagg actttgagga gacactttat cacattcctt ctgataacag caaaggcgag gttgattcaa atagcttatt tttgattgca acatctgagc aaccaatagc tgcaatgcat cataatgtga cacttgaaga taaggactta ccaatcaaat atgctggtat ttcaacatgc tttagaaaag aggcaggcgc tcatggtaaa gatacctggg gaatatttag aattcatcaa tttgaaaaag ttgagcaatt ctgtgttact ttaccagaag actcacaaaa gattcatgag gaaatgatta gcatttccga ggaattttat caatctttgg aattacctta tagagtaatt tcaatcgtat caggagcgct taatgacgct gcatcgaaaa agtatgattt agaagcttgg ttccccggat ataactcata tagggaactt gtttcatgct caaactgtac tgattaccaa tctagagcgc ttgaatgtcg tctgggattc agaaaagaag gagagcgtga aaaacattac tgccatttcc taaacggtac tttatgtgca atccaaagaa caatgtgctg tattgttgaa aactatcaaa ctccagatgg tcttagaata ccaaaagtct tacagcctta tatgaatggc gtcgaattca ttccattcaa aaacgaaacc gctacttgtg ccaattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for CrpaA.01238.a.HW1.PW38389
| PURIFICATION DATe: | 11/13/2017 |
| CONCENTRATION: | 27.87mg/ml |
| OBSERVED MW: | 67kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
3C Cleavage step completed.
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMK SDKIIHLTDD SFDTDVLKAD GAILVDFWAE WCGPCKMIAP ILDEIADEYQ GKLTVAKLNI DQNPGTAPKY GIRGIPTLLL FKNGEVAATK VGALSKGQLK EFLDANLAGT LEAQTQGPGS MPIDINRIRV EKGGDYQKIA ESEMARYKGL ETLEELVKVD QKWREDMFKL EQSKKELNSI SKEIAQIKKK DPKADCKDLQ DKSVQLKKNL PIIEKQALET EEVRDKLWHK IGNVLQPDVP ISNTEDDNLV LRTWGEIPDI KVDGTPGKLH HNEIMSRLGF YDSVKGAELA GHRGYFLKDY GVIMSMALSH YAMGFLLKKG YLAIQPPYFM KRDLMGKAAE LQDFEETLYH IPSDNSKGEV DSNSLFLIAT SEQPIAAMHH NVTLEDKDLP IKYAGISTCF RKEAGAHGKD TWGIFRIHQF EKVEQFCVTL PEDSQKIHEE MISISEEFYQ SLELPYRVIS IVSGALNDAA SKKYDLEAWF PGYNSYRELV SCSNCTDYQS RALECRLGFR KEGEREKHYC HFLNGTLCAI QRTMCCIVEN YQTPDGLRIP KVLQPYMNGV EFIPFKNETA TCAN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGAAGAGCGA TAAAATTATT CACCTGACTG ACGACAGTTT TGACACGGAT GTACTCAAAG CGGACGGGGC GATCCTCGTC GATTTCTGGG CAGAGTGGTG CGGTCCGTGC AAAATGATCG CCCCGATTCT GGATGAAATC GCTGACGAAT ATCAGGGCAA ACTGACCGTT GCAAAACTGA ACATCGATCA AAACCCTGGC ACTGCGCCGA AATATGGCAT CCGTGGTATC CCGACTCTGC TGCTGTTCAA AAACGGTGAA GTGGCGGCAA CCAAAGTGGG TGCACTGTCT AAAGGTCAGT TGAAAGAGTT CCTCGACGCT AACCTGGCCG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGATGCC TATAGATATT AATAGAATTA GAGTGGAGAA AGGTGGAGAT TATCAAAAGA TAGCCGAGTC AGAAATGGCA AGGTATAAAG GACTAGAAAC TCTTGAGGAA CTCGTTAAAG TAGATCAGAA ATGGAGAGAG GATATGTTTA AATTAGAGCA AAGCAAAAAG GAGTTAAATT CAATTTCAAA AGAAATTGCT CAAATTAAAA AGAAAGATCC AAAGGCAGAT TGCAAGGATT TACAAGACAA AAGCGTTCAA TTAAAGAAGA ATCTTCCAAT TATCGAGAAA CAAGCATTGG AAACTGAAGA AGTAAGAGAT AAGTTATGGC ATAAAATTGG TAATGTCTTA CAACCTGATG TTCCTATTTC CAATACAGAG GATGACAACC TTGTATTGAG AACATGGGGA GAAATACCAG ATATTAAGGT TGATGGAACA CCAGGGAAAT TGCATCACAA TGAAATCATG TCCAGATTAG GATTCTATGA TAGTGTGAAA GGTGCAGAAT TAGCAGGCCA TCGTGGTTAC TTTTTAAAGG ATTATGGTGT AATAATGAGT ATGGCTCTCT CACATTATGC TATGGGATTC TTGTTAAAAA AAGGATATTT GGCAATTCAA CCGCCATACT TTATGAAAAG AGATTTGATG GGTAAAGCAG CTGAATTGCA GGACTTTGAG GAGACACTTT ATCACATTCC TTCTGATAAC AGCAAAGGCG AGGTTGATTC AAATAGCTTA TTTTTGATTG CAACATCTGA GCAACCAATA GCTGCAATGC ATCATAATGT GACACTTGAA GATAAGGACT TACCAATCAA ATATGCTGGT ATTTCAACAT GCTTTAGAAA AGAGGCAGGC GCTCATGGTA AAGATACCTG GGGAATATTT AGAATTCATC AATTTGAAAA AGTTGAGCAA TTCTGTGTTA CTTTACCAGA AGACTCACAA AAGATTCATG AGGAAATGAT TAGCATTTCC GAGGAATTTT ATCAATCTTT GGAATTACCT TATAGAGTAA TTTCAATCGT ATCAGGAGCG CTTAATGACG CTGCATCGAA AAAGTATGAT TTAGAAGCTT GGTTCCCCGG ATATAACTCA TATAGGGAAC TTGTTTCATG CTCAAACTGT ACTGATTACC AATCTAGAGC GCTTGAATGT CGTCTGGGAT TCAGAAAAGA AGGAGAGCGT GAAAAACATT ACTGCCATTT CCTAAACGGT ACTTTATGTG CAATCCAAAG AACAATGTGC TGTATTGTTG AAAACTATCA AACTCCAGAT GGTCTTAGAA TACCAAAAGT CTTACAGCCT TATATGAATG GCGTCGAATT CATTCCATTC AAAAACGAAA CCGCTACTTG TGCCAATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA
Details for CrpaA.01238.a.AE1.PW38630
| PURIFICATION DATe: | 6/4/2019 |
| CONCENTRATION: | 75mg/ml |
| OBSERVED MW: | 52kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 27µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MPIDINRIRV EKGGDYQKIA ESEMARYKGL ETLEELVKVD QKWREDMFKL EQSKKELNSI SKEIAQIKKK DPKADCKDLQ DKSVQLKKNL PIIEKQALET EEVRDKLWHK IGNVLQPDVP ISNTEDDNLV LRTWGEIPDI KVDGTPGKLH HNEIMSRLGF YDSVKGAELA GHRGYFLKDY GVIMSMALSH YAMGFLLKKG YLAIQPPYFM KRDLMGKAAE LQDFEETLYH IPSDNSKGEV DSNSLFLIAT SEQPIAAMHH NVTLEDKDLP IKYAGISTCF RKEAGAHGKD TWGIFRIHQF EKVEQFCVTL PEDSQKIHEE MISISEEFYQ SLELPYRVIS IVSGALNDAA SKKYDLEAWF PGYNSYRELV SCSNCTDYQS RALECRLGFR KEGEREKHYC HFLNGTLCAI QRTMCCIVEN YQTPDGLRIP KVLQPYMNGV EFIPFKNETA TCANGHHHHH H
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GCCTATAGAT ATTAATAGAA TTAGAGTGGA GAAAGGTGGA GATTATCAAA AGATAGCCGA GTCAGAAATG GCAAGGTATA AAGGACTAGA AACTCTTGAG GAACTCGTTA AAGTAGATCA GAAATGGAGA GAGGATATGT TTAAATTAGA GCAAAGCAAA AAGGAGTTAA ATTCAATTTC AAAAGAAATT GCTCAAATTA AAAAGAAAGA TCCAAAGGCA GATTGCAAGG ATTTACAAGA CAAAAGCGTT CAATTAAAGA AGAATCTTCC AATTATCGAG AAACAAGCAT TGGAAACTGA AGAAGTAAGA GATAAGTTAT GGCATAAAAT TGGTAATGTC TTACAACCTG ATGTTCCTAT TTCCAATACA GAGGATGACA ACCTTGTATT GAGAACATGG GGAGAAATAC CAGATATTAA GGTTGATGGA ACACCAGGGA AATTGCATCA CAATGAAATC ATGTCCAGAT TAGGATTCTA TGATAGTGTG AAAGGTGCAG AATTAGCAGG CCATCGTGGT TACTTTTTAA AGGATTATGG TGTAATAATG AGTATGGCTC TCTCACATTA TGCTATGGGA TTCTTGTTAA AAAAAGGATA TTTGGCAATT CAACCGCCAT ACTTTATGAA AAGAGATTTG ATGGGTAAAG CAGCTGAATT GCAGGACTTT GAGGAGACAC TTTATCACAT TCCTTCTGAT AACAGCAAAG GCGAGGTTGA TTCAAATAGC TTATTTTTGA TTGCAACATC TGAGCAACCA ATAGCTGCAA TGCATCATAA TGTGACACTT GAAGATAAGG ACTTACCAAT CAAATATGCT GGTATTTCAA CATGCTTTAG AAAAGAGGCA GGCGCTCATG GTAAAGATAC CTGGGGAATA TTTAGAATTC ATCAATTTGA AAAAGTTGAG CAATTCTGTG TTACTTTACC AGAAGACTCA CAAAAGATTC ATGAGGAAAT GATTAGCATT TCCGAGGAAT TTTATCAATC TTTGGAATTA CCTTATAGAG TAATTTCAAT CGTATCAGGA GCGCTTAATG ACGCTGCATC GAAAAAGTAT GATTTAGAAG CTTGGTTCCC CGGATATAAC TCATATAGGG AACTTGTTTC ATGCTCAAAC TGTACTGATT ACCAATCTAG AGCGCTTGAA TGTCGTCTGG GATTCAGAAA AGAAGGAGAG CGTGAAAAAC ATTACTGCCA TTTCCTAAAC GGTACTTTAT GTGCAATCCA AAGAACAATG TGCTGTATTG TTGAAAACTA TCAAACTCCA GATGGTCTTA GAATACCAAA AGTCTTACAG CCTTATATGA ATGGCGTCGA ATTCATTCCA TTCAAAAACG AAACCGCTAC TTGTGCCAAT GGGCACCACC ATCATCATCA TTAACGGATC CGAATTCGAG CTCCGTCGAC AAGCTTGCGG CCGCACTCGA GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGAT