SSGCID: Target Page

CrpaA.01169.a

Serine/threonine protein phosphatase

CENTER ID:	CrpaA.01169.a
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.01169.a.B1.GE26965	Full length( CrpaA.01169.a )		1	322

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd7_810
RefSeq:	XP_628270.1
UniProt:	Q5CYW0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

ERQLFSRMPK GLDVYLDSLK LDREISTLLE CKTLSENEVK QLCELAKDIL EEEQNVQQIG LPLTVVGDIH GQFFDLKEIF RIGGVPPETN FLFLGDYVDR GYYSVESVTL IVALKVRYRN RVFLIRGNHE SRQITQVYGF YDECLRKYGT PNVWRYFTDL FDYLPLSALI DNKIFCPHAG LSPSLPSLDS IKSLDRIQEV PHEGPMCDLL WSDPDERYGW GVSHRGAGYT FGEDVSEMFN HTNNLQLICR AHQLILDGYQ WSHNKNVVTV FSAPNYCYRC ENQAAILRID DLGNFTFLQF DHAPEKVLPR QENQLPVPDY FV

NT Sequence

GAAAGGCAAC TTTTTTCAAG AATGCCAAAG GGCTTGGATG TATATCTGGA CTCTTTAAAA TTGGACAGAG AGATATCTAC TTTGTTAGAA TGCAAGACTC TTTCTGAAAA TGAAGTAAAA CAATTATGTG AATTAGCAAA AGATATTCTA GAAGAAGAAC AAAATGTACA GCAAATTGGA CTTCCACTAA CTGTTGTGGG TGACATACAT GGGCAATTCT TTGATCTTAA GGAAATTTTT AGGATTGGTG GGGTTCCTCC AGAAACCAAT TTTTTGTTTC TTGGTGATTA TGTCGATAGA GGTTATTATT CCGTTGAATC TGTGACTCTT ATTGTAGCCC TGAAGGTTAG ATATAGAAAT AGAGTCTTCC TTATCAGAGG TAATCATGAA AGTCGTCAAA TTACACAGGT TTACGGATTT TATGACGAGT GTCTACGGAA ATATGGAACT CCAAATGTAT GGAGGTATTT TACTGATCTT TTTGATTATC TTCCACTCTC AGCATTAATT GACAATAAGA TTTTTTGCCC ACATGCAGGT TTATCGCCGA GTCTCCCTAG CTTAGACTCT ATTAAAAGCT TGGATAGGAT TCAGGAAGTT CCTCATGAAG GGCCAATGTG TGATCTTTTA TGGTCAGACC CTGATGAACG TTATGGCTGG GGAGTATCTC ACAGAGGTGC AGGATATACT TTTGGGGAAG ATGTGAGTGA AATGTTTAAT CATACAAATA ATTTACAATT AATCTGTAGA GCCCACCAGC TAATTCTGGA TGGATATCAA TGGTCTCATA ATAAGAATGT TGTGACCGTT TTCTCAGCTC CTAATTACTG CTACAGGTGC GAAAACCAGG CGGCTATCTT AAGAATTGAT GATTTGGGAA ATTTTACATT TCTCCAATTT GACCATGCGC CTGAGAAGGT ACTTCCGAGA CAGGAAAACC AGCTTCCAGT TCCGGACTAT TTTGTT

Details for CrpaA.01169.a.B1.GE26965

HARVESTED ON:	6/18/2009
SEQUENCED ON:	7/9/2009
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

MERQLFSRMP KGLDVYLDSL KLDREISTLL ECKTLSENEV KQLCELAKDI LEEEQNVQQI GLPLTVVGDI HGQFFDLKEI FRIGGVPPET NFLFLGDYVD RGYYSVESVT LIVALKVRYR NRVFLIRGNH ESRQITQVYG FYDECLRKYG TPNVWRYFTD LFDYLPLSAL IDNKIFCPHA GLSPSLPSLD SIKSLDRIQE VPHEGPMCDL LWSDPDERYG WGVSHRGAGY TFGEDVSEMF NHTNNLQLIC RAHQLILDGY QWSHNKNVVT VFSAPNYCYR CENQAAILRI DDLGNFTFLQ FDHAPEKVLP RQE

Validated NT Sequence

atatggaaag gcaacttttt tcaagaatgc caaagggctt ggatgtatat ctggactctt taaaattgga cagagagata tctactttgt tagaatgcaa gactctttct gaaaatgaag taaaacaatt atgtgaatta gcaaaagata ttctagaaga agaacaaaat gtacagcaaa ttggacttcc actaactgtt gtgggtgaca tacatgggca attctttgat cttaaggaaa tttttaggat tggtggggtt cctccagaaa ccaatttttt gtttcttggt gattatgtcg atagaggtta ttattccgtt gaatctgtga ctcttattgt agccctgaag gttagatata gaaatagagt cttccttatc agaggtaatc atgaaagtcg tcaaattaca caggtttacg gattttatga cgagtgtcta cggaaatatg gaactccaaa tgtatggagg tattttactg atctttttga ttatcttcca ctctcagcat taattgacaa taagattttt tgcccacatg caggtttatc gccgagtctc cctagcttag actctattaa aagcttggat aggattcagg aagttcctca tgaagggcca atgtgtgatc ttttatggtc agaccctgat gaacgttatg gctggggagt atctcacaga ggtgcaggat atacttttgg ggaagatgtg agtgaaatgt ttaatcatac aaataattta caattaatct gtagagccca ccagctaatt ctggatggat atcaatggtc tcataataag aatgttgtga ccgttttctc agctcctaat tactgctaca ggtgcgaaaa ccaggcggct atcttaagaa ttgatgattt gggaaatttt acatttctcc aatttgacca tgcgcctgag aaggtacttc cgagacagga aa

Expected Protein Sequence

MAHHHHHHME RQLFSRMPKG LDVYLDSLKL DREISTLLEC KTLSENEVKQ LCELAKDILE EEQNVQQIGL PLTVVGDIHG QFFDLKEIFR IGGVPPETNF LFLGDYVDRG YYSVESVTLI VALKVRYRNR VFLIRGNHES RQITQVYGFY DECLRKYGTP NVWRYFTDLF DYLPLSALID NKIFCPHAGL SPSLPSLDSI KSLDRIQEVP HEGPMCDLLW SDPDERYGWG VSHRGAGYTF GEDVSEMFNH TNNLQLICRA HQLILDGYQW SHNKNVVTVF SAPNYCYRCE NQAAILRIDD LGNFTFLQFD HAPEKVLPRQ ENQLPVPDYF V

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa ggcaactttt ttcaagaatg ccaaagggct tggatgtata tctggactct ttaaaattgg acagagagat atctactttg ttagaatgca agactctttc tgaaaatgaa gtaaaacaat tatgtgaatt agcaaaagat attctagaag aagaacaaaa tgtacagcaa attggacttc cactaactgt tgtgggtgac atacatgggc aattctttga tcttaaggaa atttttagga ttggtggggt tcctccagaa accaattttt tgtttcttgg tgattatgtc gatagaggtt attattccgt tgaatctgtg actcttattg tagccctgaa ggttagatat agaaatagag tcttccttat cagaggtaat catgaaagtc gtcaaattac acaggtttac ggattttatg acgagtgtct acggaaatat ggaactccaa atgtatggag gtattttact gatctttttg attatcttcc actctcagca ttaattgaca ataagatttt ttgcccacat gcaggtttat cgccgagtct ccctagctta gactctatta aaagcttgga taggattcag gaagttcctc atgaagggcc aatgtgtgat cttttatggt cagaccctga tgaacgttat ggctggggag tatctcacag aggtgcagga tatacttttg gggaagatgt gagtgaaatg tttaatcata caaataattt acaattaatc tgtagagccc accagctaat tctggatgga tatcaatggt ctcataataa gaatgttgtg accgttttct cagctcctaa ttactgctac aggtgcgaaa accaggcggc tatcttaaga attgatgatt tgggaaattt tacatttctc caatttgacc atgcgcctga gaaggtactt ccgagacagg aaaaccagct tccagttccg gactattttg tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat