CrpaA.01119.a
Triosephosphate isomerase
| CENTER ID: | CrpaA.01119.a |
| ORGANISM: | Cryptosporidium parvum Iowa II |
| ASSOCIATED DISEASE: | cryptosporidiosis - (diarrheal disease) |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrpaA.01119.a.A1.GE26515 | Full length( CrpaA.01119.a ) | 1 | 250 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrpaA.01119.a.A1.PS00358 | Full length( CrpaA.01119.a ) | 1 | 250 | ||
| CrpaA.01119.a.A1.PW26550 | Full length( CrpaA.01119.a ) | 1 | 250 |
Structures
3KRS
DEPOSITED: 11/19/2009
DETERMINATION: XRay
CLONE: CrpaA.01119.a.A1.GE26515
PROTEIN: CrpaA.01119.a.A1.PS00358
Publications by SSGCID
Structure of triosephosphate isomerase from Cryptosporidium parvum.
Abendroth J, Guo W, Kelley A, Le KP, Leibly DJ, Myler PJ, Nguyen TN, Stewart L, Van Voorhis WC
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1095-9
PMID: 21904056; PMCID: PMC3169408
PDB:
3KRS
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | CryptoDB:cgd1_3040 |
| OrthoMCL: | OG5_127002 |
| RefSeq: | XP_628120.1 |
| UniProt: | Q5CSE7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRKYFVGGN FKCNGTKESL KTLIDSFKQV ESSNSEVYVF PTSLHISLVK EFFGNDHPGV FKIGSQNISC TGNGAFTGEV SCEMLKDMDV DCSLVGHSER RQYYSETDQI VNNKVKKGLE NGLKIVLCIG ESLSERETGK TNDVIQKQLT EALKDVSDLS NLVIAYEPIW AIGTGVVATP GQAQEAHAFI REYVTRMYNP QVSSNLRIIY GGSVTPDNCN ELIKCADIDG FLVGGASLKP TFAKIIESAQ
NT Sequence
ATGTCTAGAA AGTACTTTGT GGGTGGTAAC TTCAAGTGCA ATGGCACAAA AGAATCTCTA AAGACTCTGA TTGATTCTTT CAAACAAGTG GAATCAAGTA ATTCAGAGGT TTACGTATTC CCAACTTCAC TACATATTTC ATTGGTCAAA GAGTTTTTCG GTAATGATCA TCCAGGAGTA TTCAAGATTG GATCACAGAA CATTTCTTGT ACTGGAAACG GAGCATTTAC GGGAGAAGTT TCTTGTGAAA TGCTTAAAGA TATGGATGTT GATTGTTCTT TAGTAGGCCA TTCTGAAAGA AGACAATACT ATTCAGAAAC TGATCAAATC GTAAATAATA AGGTAAAGAA GGGTTTGGAG AATGGCCTGA AGATTGTTCT TTGTATTGGA GAGTCATTAA GTGAGAGAGA AACAGGAAAG ACCAATGATG TCATACAGAA GCAGTTAACG GAAGCTTTAA AGGATGTGAG TGATTTGTCT AACTTAGTAA TAGCTTACGA GCCAATTTGG GCTATCGGAA CAGGAGTAGT AGCAACACCT GGGCAAGCTC AAGAGGCTCA TGCATTTATT AGAGAATATG TTACAAGGAT GTATAATCCC CAGGTATCTT CCAATTTAAG AATAATTTAT GGAGGATCGG TCACTCCTGA TAATTGTAAT GAATTAATCA AATGCGCCGA TATTGATGGT TTCCTTGTTG GAGGAGCTTC ATTGAAGCCA ACATTTGCGA AAATCATTGA ATCTGCACAG
Details for CrpaA.01119.a.A1.GE26515
| HARVESTED ON: | 4/20/2009 |
| SEQUENCED ON: | 5/6/2009 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSRKYFVGG NFKCNGTKES LKTLIDSFKQ VESSNSEVYV FPTSLHISLV KEFFGNDHPG VFKIGSQNIS CTGNGAFTGE VSCEMLKDMD VDCSLVGHSE RRQYYSETDQ IVNNKVKKGL ENGLKIVLCI GESLSERETG KTNDVIQKQL TEALKDVSDL SNLVIAYEPI WAIGTGVVAT PGQAQEAHAF IREYVTRMYN PQVSSNLRII YGGSVTPDNC NELIKCADID GFLVGGASLK PTFAKIIESA Q
Validated NT Sequence
ttattactgt gcagattcaa tgattttcgc aaatgttggc ttcaatgaag ctcctccaac aaggaaacca tcaatatcgg cgcatttgat taattcatta caattatcag gagtgaccga tcctccataa attattctta aattggaaga tacctgggga ttatacatcc ttgtaacata ttctctaata aatgcatgag cctcttgagc ttgcccaggt gttgctacta ctcctgttcc gatagcccaa attggctcgt aagctattac taagttagac aaatcactca catcctttaa agcttccgtt aactgcttct gtatgacatc attggtcttt cctgtttctc tctcacttaa tgactctcca atacaaagaa caatcttcag gccattctcc aaacccttct ttaccttatt atttacgatt tgatcagttt ctgaatagta ttgtcttctt tcagaatggc ctactaaaga acaatcaaca tccatatctt taagcatttc acaagaaact tctcccgtaa atgctccgtt tccagtacaa gaaatgttct gtgatccaat cttgaatact cctggatgat cattaccgaa aaactctttg accaatgaaa tatgtagtga agttgggaat acgtaaacct ctgaattact tgattccact tgtttgaaag aatcaatcag agtctttaga gattcttttg tgccattgca cttgaagtta ccacccacaa agtactttct agacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRKYFVGG NFKCNGTKES LKTLIDSFKQ VESSNSEVYV FPTSLHISLV KEFFGNDHPG VFKIGSQNIS CTGNGAFTGE VSCEMLKDMD VDCSLVGHSE RRQYYSETDQ IVNNKVKKGL ENGLKIVLCI GESLSERETG KTNDVIQKQL TEALKDVSDL SNLVIAYEPI WAIGTGVVAT PGQAQEAHAF IREYVTRMYN PQVSSNLRII YGGSVTPDNC NELIKCADID GFLVGGASLK PTFAKIIESA Q
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctagaaag tactttgtgg gtggtaactt caagtgcaat ggcacaaaag aatctctaaa gactctgatt gattctttca aacaagtgga atcaagtaat tcagaggttt acgtattccc aacttcacta catatttcat tggtcaaaga gtttttcggt aatgatcatc caggagtatt caagattgga tcacagaaca tttcttgtac tggaaacgga gcatttacgg gagaagtttc ttgtgaaatg cttaaagata tggatgttga ttgttcttta gtaggccatt ctgaaagaag acaatactat tcagaaactg atcaaatcgt aaataataag gtaaagaagg gtttggagaa tggcctgaag attgttcttt gtattggaga gtcattaagt gagagagaaa caggaaagac caatgatgtc atacagaagc agttaacgga agctttaaag gatgtgagtg atttgtctaa cttagtaata gcttacgagc caatttgggc tatcggaaca ggagtagtag caacacctgg gcaagctcaa gaggctcatg catttattag agaatatgtt acaaggatgt ataatcccca ggtatcttcc aatttaagaa taatttatgg aggatcggtc actcctgata attgtaatga attaatcaaa tgcgccgata ttgatggttt ccttgttgga ggagcttcat tgaagccaac atttgcgaaa atcattgaat ctgcacagaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for CrpaA.01119.a.A1.PS00358
| PURIFICATION DATe: | 9/18/2009 |
| CONCENTRATION: | 75.3mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSRKYFVGG NFKCNGTKES LKTLIDSFKQ VESSNSEVYV FPTSLHISLV KEFFGNDHPG VFKIGSQNIS CTGNGAFTGE VSCEMLKDMD VDCSLVGHSE RRQYYSETDQ IVNNKVKKGL ENGLKIVLCI GESLSERETG KTNDVIQKQL TEALKDVSDL SNLVIAYEPI WAIGTGVVAT PGQAQEAHAF IREYVTRMYN PQVSSNLRII YGGSVTPDNC NELIKCADID GFLVGGASLK PTFAKIIESA Q
Validated NT Sequence
ttattactgt gcagattcaa tgattttcgc aaatgttggc ttcaatgaag ctcctccaac aaggaaacca tcaatatcgg cgcatttgat taattcatta caattatcag gagtgaccga tcctccataa attattctta aattggaaga tacctgggga ttatacatcc ttgtaacata ttctctaata aatgcatgag cctcttgagc ttgcccaggt gttgctacta ctcctgttcc gatagcccaa attggctcgt aagctattac taagttagac aaatcactca catcctttaa agcttccgtt aactgcttct gtatgacatc attggtcttt cctgtttctc tctcacttaa tgactctcca atacaaagaa caatcttcag gccattctcc aaacccttct ttaccttatt atttacgatt tgatcagttt ctgaatagta ttgtcttctt tcagaatggc ctactaaaga acaatcaaca tccatatctt taagcatttc acaagaaact tctcccgtaa atgctccgtt tccagtacaa gaaatgttct gtgatccaat cttgaatact cctggatgat cattaccgaa aaactctttg accaatgaaa tatgtagtga agttgggaat acgtaaacct ctgaattact tgattccact tgtttgaaag aatcaatcag agtctttaga gattcttttg tgccattgca cttgaagtta ccacccacaa agtactttct agacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRKYFVGG NFKCNGTKES LKTLIDSFKQ VESSNSEVYV FPTSLHISLV KEFFGNDHPG VFKIGSQNIS CTGNGAFTGE VSCEMLKDMD VDCSLVGHSE RRQYYSETDQ IVNNKVKKGL ENGLKIVLCI GESLSERETG KTNDVIQKQL TEALKDVSDL SNLVIAYEPI WAIGTGVVAT PGQAQEAHAF IREYVTRMYN PQVSSNLRII YGGSVTPDNC NELIKCADID GFLVGGASLK PTFAKIIESA Q
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTAGAAAG TACTTTGTGG GTGGTAACTT CAAGTGCAAT GGCACAAAAG AATCTCTAAA GACTCTGATT GATTCTTTCA AACAAGTGGA ATCAAGTAAT TCAGAGGTTT ACGTATTCCC AACTTCACTA CATATTTCAT TGGTCAAAGA GTTTTTCGGT AATGATCATC CAGGAGTATT CAAGATTGGA TCACAGAACA TTTCTTGTAC TGGAAACGGA GCATTTACGG GAGAAGTTTC TTGTGAAATG CTTAAAGATA TGGATGTTGA TTGTTCTTTA GTAGGCCATT CTGAAAGAAG ACAATACTAT TCAGAAACTG ATCAAATCGT AAATAATAAG GTAAAGAAGG GTTTGGAGAA TGGCCTGAAG ATTGTTCTTT GTATTGGAGA GTCATTAAGT GAGAGAGAAA CAGGAAAGAC CAATGATGTC ATACAGAAGC AGTTAACGGA AGCTTTAAAG GATGTGAGTG ATTTGTCTAA CTTAGTAATA GCTTACGAGC CAATTTGGGC TATCGGAACA GGAGTAGTAG CAACACCTGG GCAAGCTCAA GAGGCTCATG CATTTATTAG AGAATATGTT ACAAGGATGT ATAATCCCCA GGTATCTTCC AATTTAAGAA TAATTTATGG AGGATCGGTC ACTCCTGATA ATTGTAATGA ATTAATCAAA TGCGCCGATA TTGATGGTTT CCTTGTTGGA GGAGCTTCAT TGAAGCCAAC ATTTGCGAAA ATCATTGAAT CTGCACAGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for CrpaA.01119.a.A1.PW26550
| PURIFICATION DATe: | 6/8/2009 |
| CONCENTRATION: | 25.17mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 35 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSRKYFVGG NFKCNGTKES LKTLIDSFKQ VESSNSEVYV FPTSLHISLV KEFFGNDHPG VFKIGSQNIS CTGNGAFTGE VSCEMLKDMD VDCSLVGHSE RRQYYSETDQ IVNNKVKKGL ENGLKIVLCI GESLSERETG KTNDVIQKQL TEALKDVSDL SNLVIAYEPI WAIGTGVVAT PGQAQEAHAF IREYVTRMYN PQVSSNLRII YGGSVTPDNC NELIKCADID GFLVGGASLK PTFAKIIESA Q
Validated NT Sequence
ttattactgt gcagattcaa tgattttcgc aaatgttggc ttcaatgaag ctcctccaac aaggaaacca tcaatatcgg cgcatttgat taattcatta caattatcag gagtgaccga tcctccataa attattctta aattggaaga tacctgggga ttatacatcc ttgtaacata ttctctaata aatgcatgag cctcttgagc ttgcccaggt gttgctacta ctcctgttcc gatagcccaa attggctcgt aagctattac taagttagac aaatcactca catcctttaa agcttccgtt aactgcttct gtatgacatc attggtcttt cctgtttctc tctcacttaa tgactctcca atacaaagaa caatcttcag gccattctcc aaacccttct ttaccttatt atttacgatt tgatcagttt ctgaatagta ttgtcttctt tcagaatggc ctactaaaga acaatcaaca tccatatctt taagcatttc acaagaaact tctcccgtaa atgctccgtt tccagtacaa gaaatgttct gtgatccaat cttgaatact cctggatgat cattaccgaa aaactctttg accaatgaaa tatgtagtga agttgggaat acgtaaacct ctgaattact tgattccact tgtttgaaag aatcaatcag agtctttaga gattcttttg tgccattgca cttgaagtta ccacccacaa agtactttct agacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRKYFVGG NFKCNGTKES LKTLIDSFKQ VESSNSEVYV FPTSLHISLV KEFFGNDHPG VFKIGSQNIS CTGNGAFTGE VSCEMLKDMD VDCSLVGHSE RRQYYSETDQ IVNNKVKKGL ENGLKIVLCI GESLSERETG KTNDVIQKQL TEALKDVSDL SNLVIAYEPI WAIGTGVVAT PGQAQEAHAF IREYVTRMYN PQVSSNLRII YGGSVTPDNC NELIKCADID GFLVGGASLK PTFAKIIESA Q
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTAGAAAG TACTTTGTGG GTGGTAACTT CAAGTGCAAT GGCACAAAAG AATCTCTAAA GACTCTGATT GATTCTTTCA AACAAGTGGA ATCAAGTAAT TCAGAGGTTT ACGTATTCCC AACTTCACTA CATATTTCAT TGGTCAAAGA GTTTTTCGGT AATGATCATC CAGGAGTATT CAAGATTGGA TCACAGAACA TTTCTTGTAC TGGAAACGGA GCATTTACGG GAGAAGTTTC TTGTGAAATG CTTAAAGATA TGGATGTTGA TTGTTCTTTA GTAGGCCATT CTGAAAGAAG ACAATACTAT TCAGAAACTG ATCAAATCGT AAATAATAAG GTAAAGAAGG GTTTGGAGAA TGGCCTGAAG ATTGTTCTTT GTATTGGAGA GTCATTAAGT GAGAGAGAAA CAGGAAAGAC CAATGATGTC ATACAGAAGC AGTTAACGGA AGCTTTAAAG GATGTGAGTG ATTTGTCTAA CTTAGTAATA GCTTACGAGC CAATTTGGGC TATCGGAACA GGAGTAGTAG CAACACCTGG GCAAGCTCAA GAGGCTCATG CATTTATTAG AGAATATGTT ACAAGGATGT ATAATCCCCA GGTATCTTCC AATTTAAGAA TAATTTATGG AGGATCGGTC ACTCCTGATA ATTGTAATGA ATTAATCAAA TGCGCCGATA TTGATGGTTT CCTTGTTGGA GGAGCTTCAT TGAAGCCAAC ATTTGCGAAA ATCATTGAAT CTGCACAGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA