SSGCID: Target Page

CrpaA.01010.c

Mitogen-activated protein kinase (MAPK-1)

CENTER ID:	CrpaA.01010.c
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.01010.c.A1.GE45570	full length		1	566

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd3_3030
UniProt:	A3FQ38

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKITKNITVN VEDGSTYTVP EHYKFIKKVG SGAYGCVVSF YDKSRGSYIA VKKIFDAFQD LIDAKRILRE IKLLRQLQHE NILGIIDLLP PDSPNFEDIY IVTQLMETDL HRVIYSKQTL TNEHIQYFMY QILRGLSYLH KVNIIHRDLK PSNILVNLSC DLKICDFGLA RGNVCDIDKS KDELTDYVVT RWYRAPEIIL CVNRYDKAVD IWSAGCIFAE LIKRSALFAG HDHLDQLKAI ISCLGTPSRD DLDSWLPYKG STENARKYLD TLPNYKGRHI STLFPGFNCP EAIDLIEKML SFNPKKRITA EEALSHPYFN GININSGYDS PKSSSAHNIQ FKSNNSIEPE LISLDSNTTD SIDWSFDNFE PTKRLLQNKV YEEIADFHPE ILIRDFPFIH HLGINVPKKH LQMLRSKYAT NTLRGKDRIN AYMVDSLMST SSSTSITPSN SFDILGTIDM VKRLSIVHKQ NRMSNSQPAQ SGTIIPSNDY DVGSEGLLLH RSQELCLTTP ALQKRTYPFI SYPSDSESGN DANLNMENTV IGVGQRVKST TDNLKRIAYG KTESNI

NT Sequence

atgaaaatca caaaaaatat tactgtaaat gtagaagatg gaagcacgta cacagttccc gagcattaca agtttattaa aaaagttgga agtggtgcct atggatgtgt agtttcattt tacgataaat ctcgaggatc ttatattgca gtaaagaaga tatttgatgc atttcaggac ttaatagatg caaagagaat tctaagagaa attaagcttt taaggcagct tcaacacgaa aacattttgg gtattattga tcttcttcct ccagactctc cgaattttga agatatatat attgttactc agttaatgga gacagatttg caccgtgtga tatatagtaa acagacactg acaaatgagc atatacaata ttttatgtat caaattttga gaggattatc ttacctacat aaggtaaata ttatacaccg tgatctgaag ccatcaaata tccttgtaaa cttatcatgt gatttaaaga tttgtgattt tggcttagca agaggaaatg tctgtgatat tgataaaagc aaggacgaat taacagatta tgtagttacg agatggtaca gggcacccga aataattttg tgtgtaaata gatacgataa agctgtggat atatggagtg caggctgtat ttttgctgaa ttaattaaaa ggagtgcttt gtttgctggt catgaccatc tagatcaact taaagctatt atttcttgtt tgggtacccc aagcagagat gacttagatt cctggcttcc ttacaagggg agtaccgaga atgcgagaaa gtacttagac acgcttccaa actataaggg taggcatatt tcaactttat tccctggatt taattgccct gaagctattg accttattga aaaaatgcta agctttaatc caaaaaagcg aataacagct gaagaggctc tatcgcatcc atatttcaat ggaattaata ttaactcagg gtacgactct ccaaaatcat ctagtgctca taacattcag tttaaatcaa ataactctat tgaaccagaa ttaatctcat tggattccaa cactactgat tctattgatt ggtctttcga caactttgag ccaacaaaga gactgttaca gaataaggta tacgaagaaa ttgcagattt tcatccagag attttaattc gagactttcc gtttattcac catttaggca tcaacgtccc aaaaaagcat ctacaaatgc taaggtcaaa gtatgctaca aatacactga ggggaaaaga tagaataaat gcatacatgg ttgattctct tatgagcacg tcatcttcta cttcaattac cccttccaac agttttgata ttttaggtac aatcgatatg gttaaaaggc tgtcaatagt ccataaacaa aatagaatgt caaactctca acctgcacaa tcagggacta ttattccctc aaatgattac gacgttggat ctgaagggct tctccttcat cgttcgcaag aattatgtct tacaacgcca gcattacaaa aaagaacata tccatttata tcatacccca gtgactcaga atcaggtaat gacgcaaact tgaatatgga aaacacggtg atcggcgtgg gtcaacgtgt caagagtact actgacaatt taaaaagaat tgcctatgga aaaacagaaa gcaatatata a

Details for CrpaA.01010.c.A1.GE45570

HARVESTED ON:	8/26/2024
SEQUENCED ON:	7/31/2024
EXPECTED MW:	67kDa
OBSERVED MW:	67kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKITKNITV NVEDGSTYTV PEHYKFIKKV GSGAYGCVVS FYDKSRGSYI AVKKIFDAFQ DLIDAKRILR EIKLLRQLQH ENILGIIDLL PPDSPNFEDI YIVTQLMETD LHRVIYSKQT LTNEHIQYFM YQILRGLSYL HKVNIIHRDL KPSNILVNLS CDLKICDFGL ARGNVCDIDK SKDELTDYVV TRWYRAPEII LCVNRYDKAV DIWSAGCIFA ELIKRSALFA GHDHLDQLKA IISCLGTPSR DDLDSWLPYK GSTENARKYL DTLPNYKGRH ISTLFPGFNC PEAIDLIEKM LSFNPKKRIT AEEALSHPYF NGININSGYD SPKSSSAHNI QFKSNNSIEP ELISLDSNTT DSIDWSFDNF EPTKRLLQNK VYEEIADFHP EILIRDFPFI HHLGINVPKK HLQMLRSKYA TNTLRGKDRI NAYMVDSLMS TSSSTSITPS NSFDILGTID MVKRLSIVHK QNRMSNSQPA QSGTIIPSND YDVGSEGLLL HRSQELCLTT PALQKRTYPF ISYPSDSESG NDANLNMENT VIGVGQRVKS TTDNLKRIAY GKTESNI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaatcaca aaaaatatta ctgtaaatgt agaagatgga agcacgtaca cagttcccga gcattacaag tttattaaaa aagttggaag tggtgcctat ggatgtgtag tttcatttta cgataaatct cgaggatctt atattgcagt aaagaagata tttgatgcat ttcaggactt aatagatgca aagagaattc taagagaaat taagctttta aggcagcttc aacacgaaaa cattttgggt attattgatc ttcttcctcc agactctccg aattttgaag atatatatat tgttactcag ttaatggaga cagatttgca ccgtgtgata tatagtaaac agacactgac aaatgagcat atacaatatt ttatgtatca aattttgaga ggattatctt acctacataa ggtaaatatt atacaccgtg atctgaagcc atcaaatatc cttgtaaact tatcatgtga tttaaagatt tgtgattttg gcttagcaag aggaaatgtc tgtgatattg ataaaagcaa ggacgaatta acagattatg tagttacgag atggtacagg gcacccgaaa taattttgtg tgtaaataga tacgataaag ctgtggatat atggagtgca ggctgtattt ttgctgaatt aattaaaagg agtgctttgt ttgctggtca tgaccatcta gatcaactta aagctattat ttcttgtttg ggtaccccaa gcagagatga cttagattcc tggcttcctt acaaggggag taccgagaat gcgagaaagt acttagacac gcttccaaac tataagggta ggcatatttc aactttattc cctggattta attgccctga agctattgac cttattgaaa aaatgctaag ctttaatcca aaaaagcgaa taacagctga agaggctcta tcgcatccat atttcaatgg aattaatatt aactcagggt acgactctcc aaaatcatct agtgctcata acattcagtt taaatcaaat aactctattg aaccagaatt aatctcattg gattccaaca ctactgattc tattgattgg tctttcgaca actttgagcc aacaaagaga ctgttacaga ataaggtata cgaagaaatt gcagattttc atccagagat tttaattcga gactttccgt ttattcacca tttaggcatc aacgtcccaa aaaagcatct acaaatgcta aggtcaaagt atgctacaaa tacactgagg ggaaaagata gaataaatgc atacatggtt gattctctta tgagcacgtc atcttctact tcaattaccc cttccaacag ttttgatatt ttaggtacaa tcgatatggt taaaaggctg tcaatagtcc ataaacaaaa tagaatgtca aactctcaac ctgcacaatc agggactatt attccctcaa atgattacga cgttggatct gaagggcttc tccttcatcg ttcgcaagaa ttatgtctta caacgccagc attacaaaaa agaacatatc catttatatc ataccccagt gactcagaat caggtaatga cgcaaacttg aatatggaaa acacggtgat cggcgtgggt caacgtgtca agagtactac tgacaattta aaaagaattg cctatggaaa aacagaaagc aatatataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa