CrpaA.01010.a

Calcium/calmodulin-dependent protein kinase with a kinase domain and 2 calmodulin-like EF hands (Fragment)

CENTER ID: CrpaA.01010.a
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.01010.a.B1.GE43788 full length 1 813
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd7_40
UniProt: Q5CZ29

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKNRGKTRT PVSRQGSKPA SGLRSAQNLH SSERKGVSIG FDKLRDGVTS SIENTPVRQT SSRKVDQNSS CNRKCQSTES YRTRTKLESS ERDKAMCFSP AFKLKFSHNA FVSLGSLLEL QKKYHLKGAI GQGSYGVVRV AIENQTRAIR AIKIMNKNKI RQINPKDVER IKTEVRLMKK LHHPNIARLY EVYEDEQYIC LVMELCHGGH LLDKLNVFID DSTGKCAMDV VKTQICPCPE CNEEAINGSI HGFRESLDFV QREKLISNIM RQIFSALHYL HNQGICHRDI KPENFLFSTN KSFEIKLVDF GLSKEFYKLN NGEYYGMTTK AGTPYFVAPE VLNTTNESYG PKCDAWSAGV LLHLLLMGAV PFPGVNDADT ISQVLNKKLC FENPNYNVLS PLARDLLSNL LNRNVDERFD AMRALQHPWI SQFSDKIYKM SPIAGIEAHE SHSSRRNIFG ATEHSPVQIS MLKSLRRYYV SPLLKKIALT VIARYCPDKW IENLRNVFYK LDTRQIGKIN LYDLRVATQQ LVAQQKLKVP QHHIDLLLDA VDSDGNGEID YIEFVAAVIS PKLSCRKDVL TMAFKMLDQD EDGLISTRDI KKLVSSEKIR LSSATVLEAE EDSILEDLNV EVTKVIGKGN ENVTGISFQA FEKLLNTSYD KVCRCYHGPR EGETKGRLPV CGEDSMDTWY DDLLSCNTEN ENLTQLFAGS EPRRLQKNSL YVCRRTGFRD LLALSQGAGQ KDKFQVEMNR KRSLGNHQER EAYSTKGAKD EGQSSGEMNR EYGLGIVGFI GNGLGVRMGG NTKNPSKVLL CDS
NT Sequence
atggaaaaga accgaggaaa gactcgaaca cctgtcagtc gtcaaggctc taagcctgca agtgggctta ggagtgctca gaatcttcat agcagtgaac ggaaaggagt atcaatagga tttgataaac tcagggatgg agttacaagt tcaatagaaa acactccagt tagacaaact tcaagcagaa aagttgacca aaatagtagc tgtaatcgaa agtgtcagag caccgaaagt tatagaacaa ggactaaact tgagagctct gagagagata aggctatgtg tttttcacca gctttcaaat tgaagttcag tcacaatgca tttgtttctt taggctcatt attggagtta caaaaaaagt accatcttaa aggagcaatt ggtcaaggtt cctatggagt ggtcagagtc gcaatcgaaa atcagacgag agcaatccga gcaatcaaga taatgaataa aaacaagatt aggcaaatta atccaaagga tgtggagagg attaagacag aggttcgact aatgaagaag ttgcaccatc caaatattgc tcgtctttat gaagtttatg aagatgaaca atatatatgt ttagttatgg agctttgcca tggtggtcat ttgttggaca agcttaatgt tttcattgac gattctacag ggaagtgcgc aatggatgta gtaaaaactc aaatatgtcc ctgcccagaa tgcaatgagg aggcaatcaa tggatccatt catggattta gagaatctct agattttgtt cagagagaaa aacttatttc caatataatg cgccagattt ttagtgctct tcattatctt cataatcaag gaatttgcca tagagatatt aaacctgaaa actttttgtt ttcaaccaat aagagctttg agataaaact tgtggacttt ggcctttcta aggagtttta taaattgaac aatggagagt attatgggat gactaccaaa gccggaacac cttattttgt tgctcctgaa gttcttaata ccacaaatga atcttatggt cctaagtgtg atgcttggag tgcaggcgtg ctgctccact tattactaat gggtgcagtg cctttcccgg gagtaaatga tgcagacact atttcacagg tattaaataa aaaactttgc ttcgagaatc caaactacaa tgttctttct cctcttgcaa gagatttact ttcaaatttg ttaaatcgaa atgtagacga aagatttgat gctatgagag ctttacaaca cccatggata agccagtttt cggataaaat ttataaaatg agtcctatcg ctggaattga agctcatgaa tcacattctt ccaggagaaa catctttggg gctactgagc acagccctgt tcagatttca atgcttaaat cattaaggag atattatgtc tctcctttac taaaaaagat tgcattgact gtcattgcaa ggtattgccc agataaatgg atagaaaacc taaggaatgt attttataaa ttagatacta gacaaattgg caagattaat ctttatgatt tgcgtgttgc aactcagcag ctggtagctc aacaaaagct taaagttcct caacatcata tagatcttct cttggatgca gtagacagcg atggaaatgg agaaatcgac tatattgagt tcgttgctgc tgtaatatct ccgaaactgt cttgcaggaa ggatgtgctt actatggctt tcaaaatgtt ggatcaggat gaagatgggc ttatttctac aagagatatc aaaaaactag tcagtagtga aaaaattcga ctaagctccg caacagtact agaagctgag gaggacagta ttttggaaga tcttaatgtt gaagtaacca aagttatagg aaaaggaaat gaaaatgtca caggaatttc attccaagca tttgaaaaac tacttaatac aagctatgat aaggtttgca gatgttacca cggcccaaga gaaggggaga ccaaaggaag acttcctgtt tgtggagaag acagtatgga tacttggtat gatgacttac tctcttgcaa taccgaaaat gaaaatctaa cacagctttt tgcaggaagt gaaccaagga ggcttcaaaa aaattctctt tatgtttgca gacgcacagg atttagagat ttattagctt tgagtcaggg agcaggacaa aaggataaat ttcaagtaga aatgaacaga aagaggagtc tgggaaatca ccaggaaaga gaagcgtata gtacaaaggg agctaaagat gaagggcaaa gtagcggtga aatgaataga gaatacggct tgggtattgt ggggttcatt gggaatggat taggggtaag aatgggagga aatacaaaga acccttctaa agttctgtta tgtgacagtt aa
Details for CrpaA.01010.a.B1.GE43788
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 93kDa
OBSERVED MW: 93kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHME KNRGKTRTPV SRQGSKPASG LRSAQNLHSS ERKGVSIGFD KLRDGVTSSI ENTPVRQTSS RKVDQNSSCN RKCQSTESYR TRTKLESSER DKAMCFSPAF KLKFSHNAFV SLGSLLELQK KYHLKGAIGQ GSYGVVRVAI ENQTRAIRAI KIMNKNKIRQ INPKDVERIK TEVRLMKKLH HPNIARLYEV YEDEQYICLV MELCHGGHLL DKLNVFIDDS TGKCAMDVVK TQICPCPECN EEAINGSIHG FRESLDFVQR EKLISNIMRQ IFSALHYLHN QGICHRDIKP ENFLFSTNKS FEIKLVDFGL SKEFYKLNNG EYYGMTTKAG TPYFVAPEVL NTTNESYGPK CDAWSAGVLL HLLLMGAVPF PGVNDADTIS QVLNKKLCFE NPNYNVLSPL ARDLLSNLLN RNVDERFDAM RALQHPWISQ FSDKIYKMSP IAGIEAHESH SSRRNIFGAT EHSPVQISML KSLRRYYVSP LLKKIALTVI ARYCPDKWIE NLRNVFYKLD TRQIGKINLY DLRVATQQLV AQQKLKVPQH HIDLLLDAVD SDGNGEIDYI EFVAAVISPK LSCRKDVLTM AFKMLDQDED GLISTRDIKK LVSSEKIRLS SATVLEAEED SILEDLNVEV TKVIGKGNEN VTGISFQAFE KLLNTSYDKV CRCYHGPREG ETKGRLPVCG EDSMDTWYDD LLSCNTENEN LTQLFAGSEP RRLQKNSLYV CRRTGFRDLL ALSQGAGQKD KFQVEMNRKR SLGNHQEREA YSTKGAKDEG QSSGEMNREY GLGIVGFIGN GLGVRMGGNT KNPSKVLLCD S
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa agaaccgagg aaagactcga acacctgtca gtcgtcaagg ctctaagcct gcaagtgggc ttaggagtgc tcagaatctt catagcagtg aacggaaagg agtatcaata ggatttgata aactcaggga tggagttaca agttcaatag aaaacactcc agttagacaa acttcaagca gaaaagttga ccaaaatagt agctgtaatc gaaagtgtca gagcaccgaa agttatagaa caaggactaa acttgagagc tctgagagag ataaggctat gtgtttttca ccagctttca aattgaagtt cagtcacaat gcatttgttt ctttaggctc attattggag ttacaaaaaa agtaccatct taaaggagca attggtcaag gttcctatgg agtggtcaga gtcgcaatcg aaaatcagac gagagcaatc cgagcaatca agataatgaa taaaaacaag attaggcaaa ttaatccaaa ggatgtggag aggattaaga cagaggttcg actaatgaag aagttgcacc atccaaatat tgctcgtctt tatgaagttt atgaagatga acaatatata tgtttagtta tggagctttg ccatggtggt catttgttgg acaagcttaa tgttttcatt gacgattcta cagggaagtg cgcaatggat gtagtaaaaa ctcaaatatg tccctgccca gaatgcaatg aggaggcaat caatggatcc attcatggat ttagagaatc tctagatttt gttcagagag aaaaacttat ttccaatata atgcgccaga tttttagtgc tcttcattat cttcataatc aaggaatttg ccatagagat attaaacctg aaaacttttt gttttcaacc aataagagct ttgagataaa acttgtggac tttggccttt ctaaggagtt ttataaattg aacaatggag agtattatgg gatgactacc aaagccggaa caccttattt tgttgctcct gaagttctta ataccacaaa tgaatcttat ggtcctaagt gtgatgcttg gagtgcaggc gtgctgctcc acttattact aatgggtgca gtgcctttcc cgggagtaaa tgatgcagac actatttcac aggtattaaa taaaaaactt tgcttcgaga atccaaacta caatgttctt tctcctcttg caagagattt actttcaaat ttgttaaatc gaaatgtaga cgaaagattt gatgctatga gagctttaca acacccatgg ataagccagt tttcggataa aatttataaa atgagtccta tcgctggaat tgaagctcat gaatcacatt cttccaggag aaacatcttt ggggctactg agcacagccc tgttcagatt tcaatgctta aatcattaag gagatattat gtctctcctt tactaaaaaa gattgcattg actgtcattg caaggtattg cccagataaa tggatagaaa acctaaggaa tgtattttat aaattagata ctagacaaat tggcaagatt aatctttatg atttgcgtgt tgcaactcag cagctggtag ctcaacaaaa gcttaaagtt cctcaacatc atatagatct tctcttggat gcagtagaca gcgatggaaa tggagaaatc gactatattg agttcgttgc tgctgtaata tctccgaaac tgtcttgcag gaaggatgtg cttactatgg ctttcaaaat gttggatcag gatgaagatg ggcttatttc tacaagagat atcaaaaaac tagtcagtag tgaaaaaatt cgactaagct ccgcaacagt actagaagct gaggaggaca gtattttgga agatcttaat gttgaagtaa ccaaagttat aggaaaagga aatgaaaatg tcacaggaat ttcattccaa gcatttgaaa aactacttaa tacaagctat gataaggttt gcagatgtta ccacggccca agagaagggg agaccaaagg aagacttcct gtttgtggag aagacagtat ggatacttgg tatgatgact tactctcttg caataccgaa aatgaaaatc taacacagct ttttgcagga agtgaaccaa ggaggcttca aaaaaattct ctttatgttt gcagacgcac aggatttaga gatttattag ctttgagtca gggagcagga caaaaggata aatttcaagt agaaatgaac agaaagagga gtctgggaaa tcaccaggaa agagaagcgt atagtacaaa gggagctaaa gatgaagggc aaagtagcgg tgaaatgaat agagaatacg gcttgggtat tgtggggttc attgggaatg gattaggggt aagaatggga ggaaatacaa agaacccttc taaagttctg ttatgtgaca gttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat