CrpaA.00942.a

Putative uncharacterized protein

CENTER ID: CrpaA.00942.a
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.00942.a.B1.GE26963 Full length( CrpaA.00942.a ) 1 324
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.00942.a.B1.PW26943 Full length( CrpaA.00942.a ) 1 324

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd8_3420
RefSeq: XP_001388437.1
UniProt: A3FPZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTLDTYSRS YDDLLVHQLM LQDVERVEAY KRSFEENKEL FKGKIVLDVG CGTGILSMLA AKCGAKAVYA VDGSNISFLA KTIVEDNELS EVVKVIHGVI EEIELPVNQV DIIVSEWMGF YLLHEGMLDS VIFARDKWLN SKHGVIFPEK ASLYVSLVEI EDFWYKNIEG INNIQGFDYS SMRRIIHSSY LKSPLILGVE PKNVENFSFA RILSLDLLKI NINDLDIIKS QFTLKVKKDT EFVHGFIVWF DIEFPAHKSN AIILSTSPYD KPTHWKQTIV LFQDCLEAKA GLNLKCLSKL ERCVNESKRC YTISIEVQDA SIEF
NT Sequence
ATGTCAACTC TGGATACTTA CTCGAGGAGC TATGACGATC TTTTGGTTCA TCAATTAATG CTTCAAGATG TGGAGAGAGT AGAAGCTTAT AAACGATCAT TCGAGGAAAA CAAAGAGTTG TTCAAAGGAA AAATAGTGTT AGATGTGGGA TGTGGAACAG GAATATTGTC TATGCTTGCA GCAAAATGCG GAGCTAAAGC AGTATATGCT GTAGATGGGA GCAATATATC ATTTCTAGCA AAAACAATCG TAGAAGATAA TGAACTATCA GAGGTTGTGA AAGTCATCCA TGGGGTTATA GAAGAAATTG AGCTCCCTGT AAATCAAGTT GACATCATTG TTTCTGAATG GATGGGTTTC TATCTGTTAC ATGAAGGAAT GCTTGACAGT GTAATTTTTG CAAGAGACAA ATGGCTTAAT TCTAAGCATG GCGTAATTTT CCCTGAAAAA GCGTCGCTAT ATGTATCATT AGTAGAAATT GAAGACTTTT GGTATAAAAA CATTGAAGGA ATAAATAATA TTCAAGGATT TGATTACTCT TCAATGAGGA GGATAATACA TTCTAGTTAC TTAAAATCGC CTTTGATACT CGGCGTAGAA CCAAAAAACG TCGAAAACTT TTCATTTGCG CGTATATTAA GTTTGGATCT CTTAAAAATA AACATCAATG ATCTTGATAT TATTAAATCA CAGTTTACTC TTAAAGTAAA AAAAGATACA GAATTCGTCC ATGGCTTTAT AGTATGGTTC GATATAGAAT TCCCCGCTCA CAAGTCTAAT GCCATTATAC TTTCCACATC GCCTTATGAC AAACCTACGC ACTGGAAGCA GACAATAGTA TTATTTCAAG ATTGTTTAGA AGCAAAAGCA GGGCTAAATT TAAAATGTCT TTCAAAGCTC GAGAGGTGTG TTAATGAAAG TAAAAGGTGT TATACTATTT CTATTGAAGT CCAAGATGCA AGTATTGAGT TT
Details for CrpaA.00942.a.B1.GE26963
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/9/2009
EXPECTED MW: 37kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 91
Validated AA Sequence
MLQDVERVEA YKRSFEENKE LFKGKIVLDV GCGTGILSML AAKCGAKAVY AVDGSNISFL AKTIVEDNEL SEVVKVIHGV IEEIELPVNQ VDIIVSEWMG FYLLHEGMLD SVIFARDKWL NSKHGVIFPE KASLYVSLVE IEDFWYKNIE GINNIQGFDY SSMRRIIHSS YLKSPLILGV EPKNVENFSF ARILSLDLLK ININDLDIIK SQFTLKVKKD TEFVHGFIVW FDIEFPAHKS NAIILSTSPY DKPTHWKQTI VLFQDCLEAK AGLNLKCLSK LERCVNESKR CYTISIEVQD ASIEF
Validated NT Sequence
atcttactca cttaaaactc aatacttgca tcttggactt caatagaaat agtataacac cttttacttt cattaacaca cctctcgagc tttgaaagac attttaaatt tagccctgct tttgcttcta aacaatcttg aaataatact attgtctgct tccagtgcgt aggtttgtca taaggcgatg tggaaagtat aatggcatta gacttgtgag cggggaattc tatatcgaac catactataa agccatggac gaattctgta tcttttttta ctttaagagt aaactgtgat ttaataatat caagatcatt gatgtttatt tttaagagat ccaaacttaa tatacgcgca aatgaaaagt tttcgacgtt ttttggttct acgccgagta tcaaaggcga ttttaagtaa ctagaatgta ttatcctcct cattgaagag taatcaaatc cttgaatatt atttattcct tcaatgtttt tataccaaaa gtcttcaatt tctactaatg atacatatag cgacgctttt tcagggaaaa ttacgccatg cttagaatta agccatttgt ctcttgcaaa aattacactg tcaagcattc cttcatgtaa cagatagaaa cccatccatt cagaaacaat gatgtcaact tgatttacag ggagctcaat ttcttctata accccatgga tgactttcac aacctctgat agttcattat cttctacgat tgtttttgct agaaatgata tattgctccc atctacagca tatactgctt tagctccgca ttttgctgca agcatngaca atattcctgt tccacatccc acatctaaca ctatttttcc tttgaacaac tctttgtttt cctcgaatga tcgtttataa gcttctactc tctccacatc ttgaagcatt aattgatgaa ccaaaagatc gtcatagctc ctcgagtaag tatccagagt nnnnntatgg tggtggtggt g
Expected Protein Sequence
MAHHHHHHMS TLDTYSRSYD DLLVHQLMLQ DVERVEAYKR SFEENKELFK GKIVLDVGCG TGILSMLAAK CGAKAVYAVD GSNISFLAKT IVEDNELSEV VKVIHGVIEE IELPVNQVDI IVSEWMGFYL LHEGMLDSVI FARDKWLNSK HGVIFPEKAS LYVSLVEIED FWYKNIEGIN NIQGFDYSSM RRIIHSSYLK SPLILGVEPK NVENFSFARI LSLDLLKINI NDLDIIKSQF TLKVKKDTEF VHGFIVWFDI EFPAHKSNAI ILSTSPYDKP THWKQTIVLF QDCLEAKAGL NLKCLSKLER CVNESKRCYT ISIEVQDASI EF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa ctctggatac ttactcgagg agctatgacg atcttttggt tcatcaatta atgcttcaag atgtggagag agtagaagct tataaacgat cattcgagga aaacaaagag ttgttcaaag gaaaaatagt gttagatgtg ggatgtggaa caggaatatt gtctatgctt gcagcaaaat gcggagctaa agcagtatat gctgtagatg ggagcaatat atcatttcta gcaaaaacaa tcgtagaaga taatgaacta tcagaggttg tgaaagtcat ccatggggtt atagaagaaa ttgagctccc tgtaaatcaa gttgacatca ttgtttctga atggatgggt ttctatctgt tacatgaagg aatgcttgac agtgtaattt ttgcaagaga caaatggctt aattctaagc atggcgtaat tttccctgaa aaagcgtcgc tatatgtatc attagtagaa attgaagact tttggtataa aaacattgaa ggaataaata atattcaagg atttgattac tcttcaatga ggaggataat acattctagt tacttaaaat cgcctttgat actcggcgta gaaccaaaaa acgtcgaaaa cttttcattt gcgcgtatat taagtttgga tctcttaaaa ataaacatca atgatcttga tattattaaa tcacagttta ctcttaaagt aaaaaaagat acagaattcg tccatggctt tatagtatgg ttcgatatag aattccccgc tcacaagtct aatgccatta tactttccac atcgccttat gacaaaccta cgcactggaa gcagacaata gtattatttc aagattgttt agaagcaaaa gcagggctaa atttaaaatg tctttcaaag ctcgagaggt gtgttaatga aagtaaaagg tgttatacta tttctattga agtccaagat gcaagtattg agttttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for CrpaA.00942.a.B1.PW26943
PURIFICATION DATe: 8/4/2009
CONCENTRATION: 30.36mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 91
Protocol Notes
notes unavailable
Validated AA Sequence
MLQDVERVEA YKRSFEENKE LFKGKIVLDV GCGTGILSML AAKCGAKAVY AVDGSNISFL AKTIVEDNEL SEVVKVIHGV IEEIELPVNQ VDIIVSEWMG FYLLHEGMLD SVIFARDKWL NSKHGVIFPE KASLYVSLVE IEDFWYKNIE GINNIQGFDY SSMRRIIHSS YLKSPLILGV EPKNVENFSF ARILSLDLLK ININDLDIIK SQFTLKVKKD TEFVHGFIVW FDIEFPAHKS NAIILSTSPY DKPTHWKQTI VLFQDCLEAK AGLNLKCLSK LERCVNESKR CYTISIEVQD ASIEF
Validated NT Sequence
atcttactca cttaaaactc aatacttgca tcttggactt caatagaaat agtataacac cttttacttt cattaacaca cctctcgagc tttgaaagac attttaaatt tagccctgct tttgcttcta aacaatcttg aaataatact attgtctgct tccagtgcgt aggtttgtca taaggcgatg tggaaagtat aatggcatta gacttgtgag cggggaattc tatatcgaac catactataa agccatggac gaattctgta tcttttttta ctttaagagt aaactgtgat ttaataatat caagatcatt gatgtttatt tttaagagat ccaaacttaa tatacgcgca aatgaaaagt tttcgacgtt ttttggttct acgccgagta tcaaaggcga ttttaagtaa ctagaatgta ttatcctcct cattgaagag taatcaaatc cttgaatatt atttattcct tcaatgtttt tataccaaaa gtcttcaatt tctactaatg atacatatag cgacgctttt tcagggaaaa ttacgccatg cttagaatta agccatttgt ctcttgcaaa aattacactg tcaagcattc cttcatgtaa cagatagaaa cccatccatt cagaaacaat gatgtcaact tgatttacag ggagctcaat ttcttctata accccatgga tgactttcac aacctctgat agttcattat cttctacgat tgtttttgct agaaatgata tattgctccc atctacagca tatactgctt tagctccgca ttttgctgca agcatngaca atattcctgt tccacatccc acatctaaca ctatttttcc tttgaacaac tctttgtttt cctcgaatga tcgtttataa gcttctactc tctccacatc ttgaagcatt aattgatgaa ccaaaagatc gtcatagctc ctcgagtaag tatccagagt nnnnntatgg tggtggtggt g
Expressed Protein Sequence
MAHHHHHHMS TLDTYSRSYD DLLVHQLMLQ DVERVEAYKR SFEENKELFK GKIVLDVGCG TGILSMLAAK CGAKAVYAVD GSNISFLAKT IVEDNELSEV VKVIHGVIEE IELPVNQVDI IVSEWMGFYL LHEGMLDSVI FARDKWLNSK HGVIFPEKAS LYVSLVEIED FWYKNIEGIN NIQGFDYSSM RRIIHSSYLK SPLILGVEPK NVENFSFARI LSLDLLKINI NDLDIIKSQF TLKVKKDTEF VHGFIVWFDI EFPAHKSNAI ILSTSPYDKP THWKQTIVLF QDCLEAKAGL NLKCLSKLER CVNESKRCYT ISIEVQDASI EF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAA CTCTGGATAC TTACTCGAGG AGCTATGACG ATCTTTTGGT TCATCAATTA ATGCTTCAAG ATGTGGAGAG AGTAGAAGCT TATAAACGAT CATTCGAGGA AAACAAAGAG TTGTTCAAAG GAAAAATAGT GTTAGATGTG GGATGTGGAA CAGGAATATT GTCTATGCTT GCAGCAAAAT GCGGAGCTAA AGCAGTATAT GCTGTAGATG GGAGCAATAT ATCATTTCTA GCAAAAACAA TCGTAGAAGA TAATGAACTA TCAGAGGTTG TGAAAGTCAT CCATGGGGTT ATAGAAGAAA TTGAGCTCCC TGTAAATCAA GTTGACATCA TTGTTTCTGA ATGGATGGGT TTCTATCTGT TACATGAAGG AATGCTTGAC AGTGTAATTT TTGCAAGAGA CAAATGGCTT AATTCTAAGC ATGGCGTAAT TTTCCCTGAA AAAGCGTCGC TATATGTATC ATTAGTAGAA ATTGAAGACT TTTGGTATAA AAACATTGAA GGAATAAATA ATATTCAAGG ATTTGATTAC TCTTCAATGA GGAGGATAAT ACATTCTAGT TACTTAAAAT CGCCTTTGAT ACTCGGCGTA GAACCAAAAA ACGTCGAAAA CTTTTCATTT GCGCGTATAT TAAGTTTGGA TCTCTTAAAA ATAAACATCA ATGATCTTGA TATTATTAAA TCACAGTTTA CTCTTAAAGT AAAAAAAGAT ACAGAATTCG TCCATGGCTT TATAGTATGG TTCGATATAG AATTCCCCGC TCACAAGTCT AATGCCATTA TACTTTCCAC ATCGCCTTAT GACAAACCTA CGCACTGGAA GCAGACAATA GTATTATTTC AAGATTGTTT AGAAGCAAAA GCAGGGCTAA ATTTAAAATG TCTTTCAAAG CTCGAGAGGT GTGTTAATGA AAGTAAAAGG TGTTATACTA TTTCTATTGA AGTCCAAGAT GCAAGTATTG AGTTTTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT