CrpaA.00879.a

Actin-like

CENTER ID:	CrpaA.00879.a
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.00879.a.A1.GE26457	Full length( CrpaA.00879.a )		1	395

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd5_2330
OrthoMCL:	OG5_129186
RefSeq:	XP_626170.1
UniProt:	Q5CRL6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MATLHYDDVI TSQPAIIDNG SGTIKCGFAG EEAPRVIVNS YVGRPKYKRC MAGALEGDIF VGSKCTENRG ILSISHPITN GIITDWYDME ALWTYCFSEM KTSSEDHPVL LTEAALNPRK HREKMAEVFF EVYRVPALFI STQAVLSLYS SGRTTGVVLD SGDGVTHAVP IYQGFALSNA ITRSEIAGRN ITEYLNLQLR IAGTVFHTKS EMEIVKDIKE TACFVSSNIQ KDENDENQMG NSTFDAVTSS GNRVASGYPY KLPDGQVIYL GKERFRAPEL LFQPSLIGYE YPGIHEVLTH TINKCDLEIR RSLASQIVLA GGSTMFPGLG DRLLFELRKA LPKDVKIRIN ASSDRNLLPW IGGSILGSLA TFKTIWISKQ EFMEEGPNIV HSKTI

NT Sequence

ATGGCAACAT TACATTATGA TGATGTAATC ACAAGCCAGC CGGCTATCAT AGATAACGGT TCTGGCACGA TTAAGTGTGG ATTTGCAGGA GAAGAAGCTC CAAGAGTGAT TGTGAATAGT TATGTAGGAC GCCCAAAGTA CAAGAGATGC ATGGCAGGAG CATTAGAAGG GGATATTTTT GTGGGAAGTA AGTGCACAGA AAATAGAGGA ATTTTGTCAA TTTCTCATCC AATTACAAAT GGAATTATTA CCGACTGGTA TGATATGGAA GCTCTCTGGA CCTATTGTTT CTCTGAAATG AAAACTTCAT CGGAGGATCA TCCAGTATTA CTAACAGAAG CAGCTTTAAA CCCACGAAAA CATAGAGAGA AAATGGCAGA AGTATTTTTT GAGGTTTATA GAGTGCCTGC ATTATTTATC TCGACTCAGG CAGTTTTATC ACTTTACTCT TCAGGAAGAA CAACCGGAGT TGTCTTAGAC AGTGGGGATG GAGTGACTCA TGCTGTACCA ATATATCAAG GCTTTGCTCT AAGTAATGCA ATCACTAGAA GTGAAATTGC TGGTCGAAAT ATTACTGAGT ATCTTAATTT ACAGCTTAGA ATTGCAGGGA CAGTATTCCA TACTAAATCT GAAATGGAAA TTGTTAAAGA TATTAAAGAA ACAGCTTGTT TTGTCTCTTC TAATATTCAG AAAGATGAAA ATGATGAGAA CCAAATGGGG AATTCAACCT TTGATGCTGT TACAAGTTCC GGTAATAGAG TAGCTTCGGG CTATCCTTAT AAACTTCCAG ACGGTCAAGT AATTTACTTG GGCAAAGAAA GATTTAGAGC GCCGGAACTT CTTTTCCAGC CTAGCTTAAT TGGATATGAG TATCCAGGGA TTCATGAAGT TCTGACTCAT ACAATTAACA AGTGCGATTT GGAAATACGA AGATCTCTTG CATCTCAAAT AGTATTAGCT GGAGGGTCAA CTATGTTTCC AGGACTTGGA GATCGACTTC TTTTTGAACT TAGAAAAGCC CTTCCTAAAG ATGTTAAGAT CCGAATTAAT GCATCTAGCG ACAGAAACCT GTTGCCTTGG ATAGGAGGAT CTATTTTAGG GTCTTTAGCT ACATTTAAAA CCATTTGGAT TTCTAAGCAA GAATTTATGG AGGAAGGTCC TAATATTGTT CACTCAAAAA CCATT

Details for CrpaA.00879.a.A1.GE26457

HARVESTED ON:	4/20/2009
SEQUENCED ON:	5/6/2009
EXPECTED MW:	45kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

gaagctcaga cccagggtcc tggttcgatg gcaacattac attatgatga tgtaatcaca agccagccgg ctatcataga taacggttct ggcacgatta agtgtggatt tgcaggagaa gaagctccaa gagtgattgt gaatagttat gtaggacgcc caaagtacaa gagatgcatg gcaggagcat tagaagggga tatttttgtg ggaagtaagt gcacagaaaa tagaggaatt ttgtcaattt ctcatccaat tacaaatgga attattaccg actggtatga tatggaagct ctctggacct attgtttctc tgaaatgaaa acttcatcgg aggatcatcc agtattacta acagaagcag ctttaaaccc acgaaaacat agagagaaaa tggcagaagt attttttgag gtttatagag tgcctgcatt atttatctcg actcaggcag ttttatcact ttactcttca ggaagaacaa ccggagttgt cttagacagt ggggatggag tgactcatgc tgtaccaata tatcaaggct ttgctctaag taatgcaatc actagaagtg aaattgctgg tcgaaatatt actgagtatc ttaatttaca gcttagaatt gcagggacag tattccatac taaatctgaa atggaaattg ttaaagatat taaagaaaca gcttgttttg tctcttctaa tattcagaaa gatgaaaatg atgagaacca aatggggaat tcaacctttg atgctgttac aagttccggt aatagagtag cttcgggcta tccttataaa cttccagacg gtcaagtaat ttacttgggc aaagaaagat ttagagcgcc ggaacttctt ttccagccta gcttaattgg atatgagtat ccagggattc atgaagttct gactcataca attaacaagt gcgatttgga aatacgaaga tctcttgcat ctcaaatagt attagctgga gggtcaacta tgtttccagg acttggagat cgacttcttt ttgaacttag aaaagccctt cctaaagatg ttaagatccg aattaatgca tctagcgaca gaaacctgtt gccttggata ggaggatcta ttttagggtc tttagctaca tttaaaacca tttggatttc taagcaagaa tttatggagg aaggtcctaa tattgttcac tcaaaaacca tttaataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMATLHYDDV ITSQPAIIDN GSGTIKCGFA GEEAPRVIVN SYVGRPKYKR CMAGALEGDI FVGSKCTENR GILSISHPIT NGIITDWYDM EALWTYCFSE MKTSSEDHPV LLTEAALNPR KHREKMAEVF FEVYRVPALF ISTQAVLSLY SSGRTTGVVL DSGDGVTHAV PIYQGFALSN AITRSEIAGR NITEYLNLQL RIAGTVFHTK SEMEIVKDIK ETACFVSSNI QKDENDENQM GNSTFDAVTS SGNRVASGYP YKLPDGQVIY LGKERFRAPE LLFQPSLIGY EYPGIHEVLT HTINKCDLEI RRSLASQIVL AGGSTMFPGL GDRLLFELRK ALPKDVKIRI NASSDRNLLP WIGGSILGSL ATFKTIWISK QEFMEEGPNI VHSKTI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaacatta cattatgatg atgtaatcac aagccagccg gctatcatag ataacggttc tggcacgatt aagtgtggat ttgcaggaga agaagctcca agagtgattg tgaatagtta tgtaggacgc ccaaagtaca agagatgcat ggcaggagca ttagaagggg atatttttgt gggaagtaag tgcacagaaa atagaggaat tttgtcaatt tctcatccaa ttacaaatgg aattattacc gactggtatg atatggaagc tctctggacc tattgtttct ctgaaatgaa aacttcatcg gaggatcatc cagtattact aacagaagca gctttaaacc cacgaaaaca tagagagaaa atggcagaag tattttttga ggtttataga gtgcctgcat tatttatctc gactcaggca gttttatcac tttactcttc aggaagaaca accggagttg tcttagacag tggggatgga gtgactcatg ctgtaccaat atatcaaggc tttgctctaa gtaatgcaat cactagaagt gaaattgctg gtcgaaatat tactgagtat cttaatttac agcttagaat tgcagggaca gtattccata ctaaatctga aatggaaatt gttaaagata ttaaagaaac agcttgtttt gtctcttcta atattcagaa agatgaaaat gatgagaacc aaatggggaa ttcaaccttt gatgctgtta caagttccgg taatagagta gcttcgggct atccttataa acttccagac ggtcaagtaa tttacttggg caaagaaaga tttagagcgc cggaacttct tttccagcct agcttaattg gatatgagta tccagggatt catgaagttc tgactcatac aattaacaag tgcgatttgg aaatacgaag atctcttgca tctcaaatag tattagctgg agggtcaact atgtttccag gacttggaga tcgacttctt tttgaactta gaaaagccct tcctaaagat gttaagatcc gaattaatgc atctagcgac agaaacctgt tgccttggat aggaggatct attttagggt ctttagctac atttaaaacc atttggattt ctaagcaaga atttatggag gaaggtccta atattgttca ctcaaaaacc attaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa