SSGCID: Target Page

CrpaA.00749.b

proteasome subunit alpha type 2, putative

CENTER ID:	CrpaA.00749.b
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.00749.b.B1.GE35730	full length		1	283

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd7_3660
RefSeq:	XP_628529.1
UniProt:	Q5CY51

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

VHRTNCKLSL FIGNNHYGII NEPPFYSPHN KWQEFEYKIP QLDLFIAMDA DYSFSLTTFS PSGKLVQIEY ALNAVNSQGR PALGIKARNG VVIATEKKVA SPLVEEHSIR KVELITSEIG CCFAGMPADF RVILKKSRKI AQVYYNTYRE QIPVCELVRE IATVMQEFTQ SGGVRPFGIS LLVAGFDSTR GPQLFQVDPS GAYFGWKASA IGKDMQNAKS FLEKRYNPDV EIEDALHTAL LTLKECFEGA MNEDNIEVGI IGEDKNFTIL SPQEIKDYLG EVE

NT Sequence

gttcatcgaa ccaattgcaa attgagttta tttattggta ataatcatta tggcataatc aatgaaccgc cattttactc ccctcacaac aaatggcagg aatttgaata taaaatccct caattagatt tatttattgc tatggacgct gactactcat tttcgctcac aacattcagc ccttctggaa aacttgttca gatagaatat gcgcttaatg cagttaactc tcaagggcgt cctgcattgg gaataaaagc aagaaatgga gttgtaattg ctactgaaaa gaaagttgcg agcccattgg ttgaggaaca ttcaattaga aaggtcgaat taattactag tgagataggc tgttgctttg caggtatgcc tgctgatttc agagtgattc tgaaaaagag tcgaaaaatt gcccaggttt actacaatac gtatcgtgag caaattccag tatgtgaact agttcgagaa atcgcaactg ttatgcaaga atttactcag tcaggcggcg taagaccatt cggaatttca ttattagtag caggtttcga ttcaactaga ggcccccagc tatttcaagt agatccctct ggtgcctatt tcggatggaa ggcatctgca atcggaaaag acatgcagaa tgcaaaatct ttcttagaga aaagatataa ccctgacgta gaaattgagg atgcattaca caccgctctc ctcacattga aagagtgctt tgaaggagca atgaatgaag acaacattga agtgggtata ataggggaag acaaaaactt cactattcta tcgccacagg agataaagga ctatttaggt gaggttgaat ag

Details for CrpaA.00749.b.B1.GE35730

HARVESTED ON:	7/31/2012
SEQUENCED ON:	10/23/2013
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV HRTNCKLSLF IGNNHYGIIN EPPFYSPHNK WQEFEYKIPQ LDLFIAMDAD YSFSLTTFSP SGKLVQIEYA LNAVNSQGRP ALGIKARNGV VIATEKKVAS PLVEEHSIRK VELITSEIGC CFAGMPADFR VILKKSRKIA QVYYNTYREQ IPVCELVREI ATVMQEFTQS GGVRPFGISL LVAGFDSTRG PQLFQVDPSG AYFGWKASAI GKDMQNAKSF LEKRYNPDVE IEDALHTALL TLKECFEGAM NEDNIEVGII GEDKNFTILS PQEIKDYLGE VE

Validated NT Sequence

atggctcacc accaccacca ccatatggtt catcgaacca attgcaaatt gagtttattt attggtaata atcattatgg cataatcaat gaaccgccat tttactcccc tcacaacaaa tggcaggaat ttgaatataa aatccctcaa ttagatttat ttattgctat ggacgctgac tactcatttt cgctcacaac attcagccct tctggaaaac ttgttcagat agaatatgcg cttaatgcag ttaactctca agggcgtcct gcattgggaa taaaagcaag aaatggagtt gtaattgcta ctgaaaagaa agttgcgagc ccattggttg aggaacattc aattagaaag gtcgaattaa ttactagtga gataggctgt tgctttgcag gtatgcctgc tgatttcaga gtgattctga aaaagagtcg aaaaattgcc caggtttact acaatacgta tcgtgagcaa attccagtat gtgaactagt tcgagaaatc gcaactgtta tgcaagaatt tactcagtca ggcggcgtaa gaccattcgg aatttcatta ttagtagcag gtttcgattc aactagaggc ccccagctat ttcaagtaga tccctctggt gcctatttcg gatggaaggc atctgcaatc ggaaaagaca tgcagaatgc aaaatctttc ttagagaaaa gatataaccc tgacgtagaa attgaggatg cattacacac cgctctcctc acattgaaag agtgctttga aggagcaatg aatgaagaca acattgaagt gggtataata ggggaagaca aaaacttcac tattctatcg ccncaggaga taaaggacta tttaggtgag gttgaataan tgagtaagat aggatccggc tg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggttc atcgaaccaa ttgcaaattg agtttattta ttggtaataa tcattatggc ataatcaatg aaccgccatt ttactcccct cacaacaaat ggcaggaatt tgaatataaa atccctcaat tagatttatt tattgctatg gacgctgact actcattttc gctcacaaca ttcagccctt ctggaaaact tgttcagata gaatatgcgc ttaatgcagt taactctcaa gggcgtcctg cattgggaat aaaagcaaga aatggagttg taattgctac tgaaaagaaa gttgcgagcc cattggttga ggaacattca attagaaagg tcgaattaat tactagtgag ataggctgtt gctttgcagg tatgcctgct gatttcagag tgattctgaa aaagagtcga aaaattgccc aggtttacta caatacgtat cgtgagcaaa ttccagtatg tgaactagtt cgagaaatcg caactgttat gcaagaattt actcagtcag gcggcgtaag accattcgga atttcattat tagtagcagg tttcgattca actagaggcc cccagctatt tcaagtagat ccctctggtg cctatttcgg atggaaggca tctgcaatcg gaaaagacat gcagaatgca aaatctttct tagagaaaag atataaccct gacgtagaaa ttgaggatgc attacacacc gctctcctca cattgaaaga gtgctttgaa ggagcaatga atgaagacaa cattgaagtg ggtataatag gggaagacaa aaacttcact attctatcgc cacaggagat aaaggactat ttaggtgagg ttgaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat