CrpaA.00749.a

Pre3p/proteasome regulatory subunit beta type 6, NTN hydrolase fold

CENTER ID:	CrpaA.00749.a
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.00749.a.A1.GE26450	Full length( CrpaA.00749.a )		1	248

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd2_860
OrthoMCL:	OG5_127762
RefSeq:	XP_626319.1
UniProt:	Q5CU00

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

NIFSHNLFCE PLWRNFELVC LINEFKISKK ARTKDQKIME LDFLNGEIET GTTIVALKYK DGLVLAADGR TSTGPIIAFR AARKITQITD KVFMCRSGSA ADTQIISRYV RRIVQDHELE TGEDTKVKSV ASVARLISYQ NKEHLLADMI IAGMDPNGEF KVFRIPLGGT LIEGSYAISG SGSGYIYSML DSKYHSEMDL DECKSFARDL VSHAMFRDSS SGGIIRIIVI NKSGVEEFVV PGEDVPNL

NT Sequence

AATATATTTT CCCATAATTT ATTCTGTGAA CCTCTTTGGC GCAATTTTGA ATTAGTGTGC TTAATAAACG AATTTAAAAT AAGTAAGAAA GCTAGAACAA AGGATCAGAA AATCATGGAA CTGGATTTTT TGAATGGAGA AATTGAGACA GGAACCACAA TTGTGGCATT GAAATACAAG GATGGGTTGG TTCTAGCAGC AGATGGAAGA ACCTCCACTG GACCAATTAT TGCTTTTAGA GCAGCAAGGA AGATAACTCA AATTACTGAT AAAGTATTTA TGTGCCGTTC TGGATCTGCA GCAGACACCC AAATTATTTC TAGATATGTA CGCCGAATTG TTCAGGATCA CGAGCTTGAG ACTGGCGAAG ATACAAAAGT GAAGTCTGTC GCTTCTGTTG CTAGATTGAT ATCATATCAG AATAAAGAAC ACTTATTAGC AGATATGATC ATTGCAGGAA TGGATCCAAA TGGTGAATTT AAAGTTTTCA GGATCCCATT GGGGGGAACT TTGATTGAAG GTTCATATGC AATATCAGGA TCAGGATCAG GCTATATTTA CTCTATGTTA GACTCTAAGT ACCATTCAGA AATGGACCTT GATGAATGTA AATCTTTTGC TAGGGATTTA GTTTCCCACG CTATGTTCAG AGACTCTTCT TCTGGTGGCA TTATCAGAAT TATAGTCATT AACAAGAGTG GAGTGGAGGA ATTTGTAGTT CCTGGAGAAG ACGTTCCTAA CCTA

Details for CrpaA.00749.a.A1.GE26450

HARVESTED ON:	4/20/2009
SEQUENCED ON:	5/6/2009
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNIFSHNLF CEPLWRNFEL VCLINEFKIS KKARTKDQKI MELDFLNGEI ETGTTIVALK YKDGLVLAAD GRTSTGPIIA FRAARKITQI TDKVFMCRSG SAADTQIISR YVRRIVQDHE LETGEDTKVK SVASVARLIS YQNKEHLLAD MIIAGMDPNG EFKVFRIPLG GTLIEGSYAI SGSGSGYIYS MLDSKYHSEM DLDECKSFAR DLVSHAMFRD SSSGGIIRII VINKSGVEEF VVPGEDVP

Validated NT Sequence

taggaacgtc ttctccagga actacaaatt cctccactcc actcttgtta atgactataa ttctgataat gccaccagaa gaagagtctc tgaacatagc gtgggaaact aaatccctag caaaagattt acattcatca aggtccattt ctgaatggta cttagagtct aacatagagt aaatatagcc tgatcctgat cctgatattg catatgaacc ttcaatcaaa gttcccccca atgggatcct gaaaacttta aattcaccat ttggatccat tcctgcaatg atcatatctg ctaataagtg ttctttattc tgatatgata tcaatctagc aacagaagcg acagacttca cttttgtatc ttcgccagtc tcaagctcgt gatcctgaac aattcggcgt acatatctag aaataatttg ggtgtctgct gcagatccag aacggcacat aaatacttta tcagtaattt gagttatctt ccttgctgct ctaaaagcaa taattggtcc agtggaggtt cttccatctg ctgctagaac caacccatcc ttgtatttca atgccacaat tgtggttcct gtctcaattt ctccattcaa aaaatccagt tccatgattt tctgatcctt tgttctagct ttcttactta ttttaaattc gtttattaag cacactaatt caaaattgcg ccaaagaggt tcacagaata aattatggga aaatatattc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatatattt tcccataatt tattctgtga acctctttgg cgcaattttg aattagtgtg cttaataaac gaatttaaaa taagtaagaa agctagaaca aaggatcaga aaatcatgga actggatttt ttgaatggag aaattgagac aggaaccaca attgtggcat tgaaatacaa ggatgggttg gttctagcag cagatggaag aacctccact ggaccaatta ttgcttttag agcagcaagg aagataactc aaattactga taaagtattt atgtgccgtt ctggatctgc agcagacacc caaattattt ctagatatgt acgccgaatt gttcaggatc acgagcttga gactggcgaa gatacaaaag tgaagtctgt cgcttctgtt gctagattga tatcatatca gaataaagaa cacttattag cagatatgat cattgcagga atggatccaa atggtgaatt taaagttttc aggatcccat tggggggaac tttgattgaa ggttcatatg caatatcagg atcaggatca ggctatattt actctatgtt agactctaag taccattcag aaatggacct tgatgaatgt aaatcttttg ctagggattt agtttcccac gctatgttca gagactcttc ttctggtggc attatcagaa ttatagtcat taacaagagt ggagtggagg aatttgtagt tcctggagaa gacgttccta acctaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa