CrpaA.00721.a

Inosine-5-monophosphate dehydrogenase

CENTER ID: CrpaA.00721.a
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.00721.a.A1.GE26449 Full length( CrpaA.00721.a ) 1 402
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrpaA.00721.a.A1.PS00363 Full length( CrpaA.00721.a ) 1 402
External Resources
RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd6_20
OrthoMCL: OG5_126887
RefSeq: XP_625342.1
UniProt: Q5CPK7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
SNMGTKNIGK GLTFEDILLV PNYSEVLPRE VSLETKLTKN VSLKIPLISS AMDTVTEHLM AVGMARLGGI GIIHKNMDME SQVNEVLKVK NWISNLEKNE STPDQNLDKE STDGKDTKSN NNIDAYSNEN LDNKGRLRVG AAIGVNEIER AKLLVEAGVD VIVLDSAHGH SLNIIRTLKE IKSKMNIDVI VGNVVTEEAT KELIENGADG IKVGIGPGSI CTTRIVAGVG VPQITAIEKC SSVASKFGIP IIADGGIRYS GDIGKALAVG ASSVMIGSIL AGTEESPGEK ELIGDTVYKY YRGMGSVGAM KSGSGDRYFQ EKRPENKMVP EGIEGRVKYK GEMEGVVYQL VGGLRSCMGY LGSASIEELW KKSSYVEITT SGLRESHVHD VEIVKEVMNY SK
NT Sequence
TCAAACATGG GTACAAAAAA CATAGGAAAA GGCTTAACAT TCGAAGACAT TTTGTTGGTT CCAAATTATT CAGAGGTACT ACCAAGAGAA GTTAGCTTAG AAACAAAATT AACAAAAAAT GTTTCTCTAA AAATCCCTCT GATAAGCTCT GCAATGGATA CAGTAACAGA ACATTTAATG GCAGTAGGAA TGGCGAGACT AGGAGGGATT GGAATTATTC ACAAAAATAT GGATATGGAA AGTCAGGTGA ATGAAGTATT GAAAGTCAAG AATTGGATAT CTAATTTAGA AAAGAATGAA AGTACTCCAG ATCAAAATTT AGACAAAGAA AGTACAGATG GAAAAGATAC AAAGTCTAAC AATAATATTG ATGCTTATAG CAATGAAAAT CTTGATAATA AAGGTAGATT AAGAGTTGGA GCAGCAATAG GTGTAAATGA AATAGAAAGA GCAAAATTAT TAGTAGAAGC AGGAGTAGAT GTGATTGTAT TAGACTCAGC GCATGGCCAT TCCCTTAACA TTATCAGAAC TTTGAAAGAA ATTAAGTCAA AAATGAATAT TGATGTAATT GTTGGGAATG TTGTAACAGA AGAAGCAACA AAAGAATTAA TTGAGAATGG TGCAGATGGG ATAAAGGTTG GAATTGGTCC AGGAAGTATT TGTACCACAA GAATTGTTGC AGGAGTTGGT GTTCCTCAAA TCACTGCAAT TGAGAAATGT TCATCAGTTG CCAGTAAATT TGGTATTCCG ATCATTGCAG ATGGAGGAAT AAGATATAGT GGTGATATTG GAAAAGCATT AGCTGTTGGA GCTTCAAGTG TAATGATTGG TTCAATCCTT GCTGGCACTG AAGAAAGTCC AGGTGAAAAA GAACTTATAG GCGATACTGT TTATAAGTAT TATAGAGGCA TGGGAAGTGT TGGAGCAATG AAGAGTGGTA GTGGGGATAG ATATTTCCAA GAGAAAAGAC CTGAAAACAA GATGGTTCCA GAAGGTATTG AAGGTAGAGT TAAATATAAA GGTGAAATGG AAGGTGTAGT ATATCAGCTT GTTGGAGGAC TAAGATCCTG CATGGGCTAT TTAGGTTCTG CCTCAATTGA AGAATTATGG AAGAAATCAA GCTATGTTGA AATAACAACA TCTGGATTAA GAGAAAGTCA TGTTCATGAT GTTGAAATCG TAAAAGAAGT AATGAATTAT AGTAAA
Details for CrpaA.00721.a.A1.GE26449
HARVESTED ON: 4/20/2009
SEQUENCED ON: 5/6/2009
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSNMGTKNI GKGLTFEDIL LVPNYSEVLP REVSLETKLT KNVSLKIPLI SSAMDTVTEH LMAVGMARLG GIGIIHKNMD MESQVNEVLK VKNWISNLEK NESTPDQNLD KESTDGKDTK SNNNIDAYSN ENLDNKGRLR VGAAIGVNEI ERAKLLVEAG VDVIVLDSAH GHSLNIIRTL KEIKSKMNID VIVGNVVTEE ATKELIENGA DGIKVGIGPG SICTTRIVAG VGVPQITAIE KCSSVASKFG IPIIADGGIR YSGDIGKALA VGASSVMIGS ILAGTEESPG EKELIGDTVY KYYRGMGSVG AMKSGSGDRY FQEKRPENKM VPEGIEGRVK YKGEMEGVVY QLVGGLRSCM GYLGSASIEE LWKKSSYVEI TTSGLRESHV HDVEIVKEVM NYSK
Validated NT Sequence
ttaagaagga gnnntaccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgtcaaac atgggtacaa aaaacatagg aaaaggctta acattcgaag acattttgtt ggttccaaat tattcagagg tactaccaag agaagttagc ttagaaacaa aattaacaaa aaatgtttct ctaaaaatcc ctctgataag ctctgcaatg gatacagtaa cagaacattt aatggcagta ggaatggcga gactaggagg gattggaatt attcacaaaa atatggatat ggaaagtcag gtgaatgaag tattgaaagt caagaattgg atatctaatt tagaaaagaa tgaaagtact ccagatcaaa atttagacaa agaaagtaca gatggaaaag atacaaagtc taacaataat attgatgctt atagcaatga aaatcttgat aataaaggta gattaagagt tggagcagca ataggtgtaa atgaaataga aagagcaaaa ttattagtag aagcaggagt agatgtgatt gtattagact cagcgcatgg ccattccctt aacattatca gaactttgaa agaaattaag tcaaaaatga atattgatgt aattgttggg aatgttgtaa cagaagaagc aacaaaagaa ttaattgaga atggtgcaga tgggataaag gttggaattg gtccaggaag tatttgtacc acaagaattg ttgcaggagt tggtgttcct caaatcactg caattgagaa atgttcatca gttgccagta aatttggtat tccgatcatt gcagatggag gaataagata tagtggtgat attggaaaag cattagctgt tggagcttca agtgtaatga ttggttcaat ccttgctggc actgaagaaa gtccaggtga aaaagaactt ataggcgata ctgtttataa gtattataga ggcatgggaa gtgttggagc aatgaagagt ggtagtgggg atagatattt ccaagagaaa agacctgaaa acaagatggt tccagaaggt attgaaggta gagttaaata taaaggtgaa atggaaggtg tagtatatca gcttgttgga ggactaagat cctgcatggg ctatttaggt tctgcctcaa ttgaagaatt atggaagaaa tcaagctatg ttgaaataac aacatctgga ttaagagaaa gtcatgttca tgatgttgaa atcgtaaaag aagtaatgaa ttatagtaaa taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNMGTKNI GKGLTFEDIL LVPNYSEVLP REVSLETKLT KNVSLKIPLI SSAMDTVTEH LMAVGMARLG GIGIIHKNMD MESQVNEVLK VKNWISNLEK NESTPDQNLD KESTDGKDTK SNNNIDAYSN ENLDNKGRLR VGAAIGVNEI ERAKLLVEAG VDVIVLDSAH GHSLNIIRTL KEIKSKMNID VIVGNVVTEE ATKELIENGA DGIKVGIGPG SICTTRIVAG VGVPQITAIE KCSSVASKFG IPIIADGGIR YSGDIGKALA VGASSVMIGS ILAGTEESPG EKELIGDTVY KYYRGMGSVG AMKSGSGDRY FQEKRPENKM VPEGIEGRVK YKGEMEGVVY QLVGGLRSCM GYLGSASIEE LWKKSSYVEI TTSGLRESHV HDVEIVKEVM NYSK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcaaacatg ggtacaaaaa acataggaaa aggcttaaca ttcgaagaca ttttgttggt tccaaattat tcagaggtac taccaagaga agttagctta gaaacaaaat taacaaaaaa tgtttctcta aaaatccctc tgataagctc tgcaatggat acagtaacag aacatttaat ggcagtagga atggcgagac taggagggat tggaattatt cacaaaaata tggatatgga aagtcaggtg aatgaagtat tgaaagtcaa gaattggata tctaatttag aaaagaatga aagtactcca gatcaaaatt tagacaaaga aagtacagat ggaaaagata caaagtctaa caataatatt gatgcttata gcaatgaaaa tcttgataat aaaggtagat taagagttgg agcagcaata ggtgtaaatg aaatagaaag agcaaaatta ttagtagaag caggagtaga tgtgattgta ttagactcag cgcatggcca ttcccttaac attatcagaa ctttgaaaga aattaagtca aaaatgaata ttgatgtaat tgttgggaat gttgtaacag aagaagcaac aaaagaatta attgagaatg gtgcagatgg gataaaggtt ggaattggtc caggaagtat ttgtaccaca agaattgttg caggagttgg tgttcctcaa atcactgcaa ttgagaaatg ttcatcagtt gccagtaaat ttggtattcc gatcattgca gatggaggaa taagatatag tggtgatatt ggaaaagcat tagctgttgg agcttcaagt gtaatgattg gttcaatcct tgctggcact gaagaaagtc caggtgaaaa agaacttata ggcgatactg tttataagta ttatagaggc atgggaagtg ttggagcaat gaagagtggt agtggggata gatatttcca agagaaaaga cctgaaaaca agatggttcc agaaggtatt gaaggtagag ttaaatataa aggtgaaatg gaaggtgtag tatatcagct tgttggagga ctaagatcct gcatgggcta tttaggttct gcctcaattg aagaattatg gaagaaatca agctatgttg aaataacaac atctggatta agagaaagtc atgttcatga tgttgaaatc gtaaaagaag taatgaatta tagtaaaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for CrpaA.00721.a.A1.PS00363
PURIFICATION DATe: 9/23/2009
CONCENTRATION: 32mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSNMGTKNI GKGLTFEDIL LVPNYSEVLP REVSLETKLT KNVSLKIPLI SSAMDTVTEH LMAVGMARLG GIGIIHKNMD MESQVNEVLK VKNWISNLEK NESTPDQNLD KESTDGKDTK SNNNIDAYSN ENLDNKGRLR VGAAIGVNEI ERAKLLVEAG VDVIVLDSAH GHSLNIIRTL KEIKSKMNID VIVGNVVTEE ATKELIENGA DGIKVGIGPG SICTTRIVAG VGVPQITAIE KCSSVASKFG IPIIADGGIR YSGDIGKALA VGASSVMIGS ILAGTEESPG EKELIGDTVY KYYRGMGSVG AMKSGSGDRY FQEKRPENKM VPEGIEGRVK YKGEMEGVVY QLVGGLRSCM GYLGSASIEE LWKKSSYVEI TTSGLRESHV HDVEIVKEVM NYSK
Validated NT Sequence
ttaagaagga gnnntaccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgtcaaac atgggtacaa aaaacatagg aaaaggctta acattcgaag acattttgtt ggttccaaat tattcagagg tactaccaag agaagttagc ttagaaacaa aattaacaaa aaatgtttct ctaaaaatcc ctctgataag ctctgcaatg gatacagtaa cagaacattt aatggcagta ggaatggcga gactaggagg gattggaatt attcacaaaa atatggatat ggaaagtcag gtgaatgaag tattgaaagt caagaattgg atatctaatt tagaaaagaa tgaaagtact ccagatcaaa atttagacaa agaaagtaca gatggaaaag atacaaagtc taacaataat attgatgctt atagcaatga aaatcttgat aataaaggta gattaagagt tggagcagca ataggtgtaa atgaaataga aagagcaaaa ttattagtag aagcaggagt agatgtgatt gtattagact cagcgcatgg ccattccctt aacattatca gaactttgaa agaaattaag tcaaaaatga atattgatgt aattgttggg aatgttgtaa cagaagaagc aacaaaagaa ttaattgaga atggtgcaga tgggataaag gttggaattg gtccaggaag tatttgtacc acaagaattg ttgcaggagt tggtgttcct caaatcactg caattgagaa atgttcatca gttgccagta aatttggtat tccgatcatt gcagatggag gaataagata tagtggtgat attggaaaag cattagctgt tggagcttca agtgtaatga ttggttcaat ccttgctggc actgaagaaa gtccaggtga aaaagaactt ataggcgata ctgtttataa gtattataga ggcatgggaa gtgttggagc aatgaagagt ggtagtgggg atagatattt ccaagagaaa agacctgaaa acaagatggt tccagaaggt attgaaggta gagttaaata taaaggtgaa atggaaggtg tagtatatca gcttgttgga ggactaagat cctgcatggg ctatttaggt tctgcctcaa ttgaagaatt atggaagaaa tcaagctatg ttgaaataac aacatctgga ttaagagaaa gtcatgttca tgatgttgaa atcgtaaaag aagtaatgaa ttatagtaaa taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNMGTKNI GKGLTFEDIL LVPNYSEVLP REVSLETKLT KNVSLKIPLI SSAMDTVTEH LMAVGMARLG GIGIIHKNMD MESQVNEVLK VKNWISNLEK NESTPDQNLD KESTDGKDTK SNNNIDAYSN ENLDNKGRLR VGAAIGVNEI ERAKLLVEAG VDVIVLDSAH GHSLNIIRTL KEIKSKMNID VIVGNVVTEE ATKELIENGA DGIKVGIGPG SICTTRIVAG VGVPQITAIE KCSSVASKFG IPIIADGGIR YSGDIGKALA VGASSVMIGS ILAGTEESPG EKELIGDTVY KYYRGMGSVG AMKSGSGDRY FQEKRPENKM VPEGIEGRVK YKGEMEGVVY QLVGGLRSCM GYLGSASIEE LWKKSSYVEI TTSGLRESHV HDVEIVKEVM NYSK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCAAACATG GGTACAAAAA ACATAGGAAA AGGCTTAACA TTCGAAGACA TTTTGTTGGT TCCAAATTAT TCAGAGGTAC TACCAAGAGA AGTTAGCTTA GAAACAAAAT TAACAAAAAA TGTTTCTCTA AAAATCCCTC TGATAAGCTC TGCAATGGAT ACAGTAACAG AACATTTAAT GGCAGTAGGA ATGGCGAGAC TAGGAGGGAT TGGAATTATT CACAAAAATA TGGATATGGA AAGTCAGGTG AATGAAGTAT TGAAAGTCAA GAATTGGATA TCTAATTTAG AAAAGAATGA AAGTACTCCA GATCAAAATT TAGACAAAGA AAGTACAGAT GGAAAAGATA CAAAGTCTAA CAATAATATT GATGCTTATA GCAATGAAAA TCTTGATAAT AAAGGTAGAT TAAGAGTTGG AGCAGCAATA GGTGTAAATG AAATAGAAAG AGCAAAATTA TTAGTAGAAG CAGGAGTAGA TGTGATTGTA TTAGACTCAG CGCATGGCCA TTCCCTTAAC ATTATCAGAA CTTTGAAAGA AATTAAGTCA AAAATGAATA TTGATGTAAT TGTTGGGAAT GTTGTAACAG AAGAAGCAAC AAAAGAATTA ATTGAGAATG GTGCAGATGG GATAAAGGTT GGAATTGGTC CAGGAAGTAT TTGTACCACA AGAATTGTTG CAGGAGTTGG TGTTCCTCAA ATCACTGCAA TTGAGAAATG TTCATCAGTT GCCAGTAAAT TTGGTATTCC GATCATTGCA GATGGAGGAA TAAGATATAG TGGTGATATT GGAAAAGCAT TAGCTGTTGG AGCTTCAAGT GTAATGATTG GTTCAATCCT TGCTGGCACT GAAGAAAGTC CAGGTGAAAA AGAACTTATA GGCGATACTG TTTATAAGTA TTATAGAGGC ATGGGAAGTG TTGGAGCAAT GAAGAGTGGT AGTGGGGATA GATATTTCCA AGAGAAAAGA CCTGAAAACA AGATGGTTCC AGAAGGTATT GAAGGTAGAG TTAAATATAA AGGTGAAATG GAAGGTGTAG TATATCAGCT TGTTGGAGGA CTAAGATCCT GCATGGGCTA TTTAGGTTCT GCCTCAATTG AAGAATTATG GAAGAAATCA AGCTATGTTG AAATAACAAC ATCTGGATTA AGAGAAAGTC ATGTTCATGA TGTTGAAATC GTAAAAGAAG TAATGAATTA TAGTAAAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA