SSGCID: Target Page

CrpaA.00697.a

NifS-like protein; cysteine desulfurase

CENTER ID:	CrpaA.00697.a
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.00697.a.A1.GE26445	Full length( CrpaA.00697.a )		1	438

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd4_3040
RefSeq:	XP_625888.1
UniProt:	Q5CQW4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIVHRYCRQW APSVVRGISK LAFFSSMSSI AKKRPAYFDY QATTPVDPRV LDKMMPFFTE KFGNSHSRTH GYGWEAEEAV ENARTNIANL IKCLPKEIIF TSGATESNNT IIRGVCDIYG DIENKKNHII TTQIEHKCVL STLRELELKG FRVTYLKVNN KGLISLEELE KSIIPGETIL ASIMHVNNEI GVIQPMNLIG EICKKYNVLF HSDVAQGLGK INIDVDKWNA DFLSLSAHKV YGPKGIGAFY IRSKPRRRIK PLIFGGGQER GMRSGTMPVP LAVGFGEACK IASSEMNSDS IHVKSLYDKL YKGITTQLPD VELNGCGVNR MFGNLNLSFT GVEGESLMMK LYSLALSSGS ACTSASLEPS YVLRAIGVGE DVAHTSIRFG LGRFTKHEDV DKAVKEIVES VTLLRKMSPL WDSITDTKVK DEESLKWT

NT Sequence

ATGATCGTTC ACAGATATTG CAGGCAATGG GCACCAAGCG TTGTAAGAGG TATCTCGAAG CTTGCATTTT TTTCATCGAT GAGTTCCATT GCTAAAAAGA GACCAGCATA CTTTGATTAC CAAGCTACGA CGCCTGTTGA CCCAAGAGTT TTAGATAAAA TGATGCCTTT TTTTACGGAA AAGTTTGGAA ACTCTCATTC TAGAACTCAT GGGTATGGAT GGGAAGCAGA AGAAGCTGTG GAGAATGCAA GAACTAATAT TGCGAACCTT ATTAAATGCT TGCCCAAAGA AATAATCTTT ACTTCAGGAG CAACTGAATC TAATAATACG ATAATTAGGG GTGTATGCGA CATTTATGGC GATATAGAAA ATAAAAAGAA TCACATTATT ACTACTCAAA TTGAACACAA ATGTGTTCTG TCAACATTAA GAGAACTTGA ACTAAAAGGT TTCAGGGTGA CTTATCTTAA AGTCAACAAT AAAGGCCTTA TTTCTTTAGA GGAGCTGGAA AAGTCCATAA TCCCTGGAGA GACTATTCTG GCTTCTATTA TGCATGTCAA CAATGAGATT GGAGTAATTC AGCCTATGAA TTTAATAGGA GAAATCTGTA AAAAATATAA TGTTCTTTTC CATAGTGATG TAGCCCAGGG ACTAGGAAAA ATCAATATTG ATGTTGATAA GTGGAATGCA GACTTTTTAT CTCTTTCCGC ACATAAGGTG TACGGTCCGA AAGGAATAGG AGCATTTTAT ATTCGTAGTA AGCCTAGAAG ACGTATAAAA CCACTAATTT TTGGTGGCGG CCAGGAGCGA GGAATGAGAT CTGGCACGAT GCCAGTACCA TTAGCTGTTG GATTTGGTGA AGCATGTAAG ATTGCTTCTT CTGAAATGAA TTCAGATTCT ATACACGTCA AGTCTCTATA TGATAAACTT TACAAAGGAA TTACCACTCA ACTACCAGAT GTAGAACTCA ACGGGTGTGG CGTAAACCGT ATGTTTGGGA ACCTAAACTT AAGTTTTACT GGAGTTGAAG GGGAATCACT AATGATGAAG CTATATTCTT TGGCTTTATC ATCCGGAAGT GCATGCACAT CTGCATCATT GGAACCATCG TACGTTTTAA GAGCAATCGG TGTCGGAGAA GATGTTGCCC ATACATCCAT TCGCTTTGGA CTGGGGAGGT TCACTAAACA CGAAGATGTA GATAAGGCTG TTAAAGAAAT TGTCGAGTCT GTAACTCTAT TGAGAAAGAT GTCCCCTCTT TGGGATTCTA TTACAGACAC AAAGGTTAAA GATGAGGAGT CACTCAAATG GACG

Details for CrpaA.00697.a.A1.GE26445

HARVESTED ON:	4/20/2009
SEQUENCED ON:	5/6/2009
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

Validated NT Sequence

acgtccattt gagtgactcc tcatctttaa cctttgtgtc tgtaatagaa tcccaaagag gggacatctt tctcaataga gttacagact cgacaatttc tttaacagcc ttatctacat cttcgtgttt agtgaacctc cccagtccaa agcgaatgga tgtatgggca acatcttctc cgacaccgat tgctcttaaa acgtacgatg gttccaatga tgcagatgtg catgcacttc cggatgataa agccaaagaa tatagcttca tcattagtga ttccccttca actccagtaa aacttaagtt taggttccca aacatacggt ttacgccaca cccgttgagt tctacatctg gtagttgagt ggtaattcct ttgtaaagtt tatcatatag agacttgacg tgtatagaat ctgaattcat ttcagaagaa gcaatcttac atgcttcacc aaatccaaca gctaatggta ctggcatcgt gccagatctc attcctcgct cctggccgcc accaaaaatt agtggtttta tacgtcttct aggcttacta cgaatataaa atgctcctat tcctttcgga ccgtacacct tatgtgcgga aagagataaa aagtctgcat tccacttatc aacatcaata ttgatttttc ctagtccctg ggctacatca ctatggaaaa gaacattata ttttttacag atttctccta ttaaattcat aggctgaatt actccaatct cattgttgac atgcataata gaagccagaa tagtctctcc agggattatg gacttttcca gctcctctaa agaaataagg cctttattgt tgactttaag ataagtcacc ctgaaacctt ttagttcaag ttctcttaat gttgacagaa cacatttgtg ttcaatttga gtagtaataa tgtgattctt tttattttct atatcgccat aaatgtcgca tacaccccta attatcgtat tattagattc agttgctcct gaagtaaaga ttatttcttt gggcaagcat ttaataaggt tcgcaatatt agttcttgca ttctccacag cttcttctgc ttcccatcca tacccatgag ttctagaatg agagtttcca aacttttccg taaaaaaagg catcatttta tctaaaactc ttgggtcaac aggcgtcgta gcttggtaat caaagtatgc tggtctcttt ttagcaatgg aactcatcga tgaaaaaaat gcaagcttcg agatacctct tacaacgctt ggtgcccatt gcctgcaata tctgtgaacg atcatcgaac caggaccctg ggtctgagct tccaggg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIVHRYCRQ WAPSVVRGIS KLAFFSSMSS IAKKRPAYFD YQATTPVDPR VLDKMMPFFT EKFGNSHSRT HGYGWEAEEA VENARTNIAN LIKCLPKEII FTSGATESNN TIIRGVCDIY GDIENKKNHI ITTQIEHKCV LSTLRELELK GFRVTYLKVN NKGLISLEEL EKSIIPGETI LASIMHVNNE IGVIQPMNLI GEICKKYNVL FHSDVAQGLG KINIDVDKWN ADFLSLSAHK VYGPKGIGAF YIRSKPRRRI KPLIFGGGQE RGMRSGTMPV PLAVGFGEAC KIASSEMNSD SIHVKSLYDK LYKGITTQLP DVELNGCGVN RMFGNLNLSF TGVEGESLMM KLYSLALSSG SACTSASLEP SYVLRAIGVG EDVAHTSIRF GLGRFTKHED VDKAVKEIVE SVTLLRKMSP LWDSITDTKV KDEESLKWT

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcgttcac agatattgca ggcaatgggc accaagcgtt gtaagaggta tctcgaagct tgcatttttt tcatcgatga gttccattgc taaaaagaga ccagcatact ttgattacca agctacgacg cctgttgacc caagagtttt agataaaatg atgccttttt ttacggaaaa gtttggaaac tctcattcta gaactcatgg gtatggatgg gaagcagaag aagctgtgga gaatgcaaga actaatattg cgaaccttat taaatgcttg cccaaagaaa taatctttac ttcaggagca actgaatcta ataatacgat aattaggggt gtatgcgaca tttatggcga tatagaaaat aaaaagaatc acattattac tactcaaatt gaacacaaat gtgttctgtc aacattaaga gaacttgaac taaaaggttt cagggtgact tatcttaaag tcaacaataa aggccttatt tctttagagg agctggaaaa gtccataatc cctggagaga ctattctggc ttctattatg catgtcaaca atgagattgg agtaattcag cctatgaatt taataggaga aatctgtaaa aaatataatg ttcttttcca tagtgatgta gcccagggac taggaaaaat caatattgat gttgataagt ggaatgcaga ctttttatct ctttccgcac ataaggtgta cggtccgaaa ggaataggag cattttatat tcgtagtaag cctagaagac gtataaaacc actaattttt ggtggcggcc aggagcgagg aatgagatct ggcacgatgc cagtaccatt agctgttgga tttggtgaag catgtaagat tgcttcttct gaaatgaatt cagattctat acacgtcaag tctctatatg ataaacttta caaaggaatt accactcaac taccagatgt agaactcaac gggtgtggcg taaaccgtat gtttgggaac ctaaacttaa gttttactgg agttgaaggg gaatcactaa tgatgaagct atattctttg gctttatcat ccggaagtgc atgcacatct gcatcattgg aaccatcgta cgttttaaga gcaatcggtg tcggagaaga tgttgcccat acatccattc gctttggact ggggaggttc actaaacacg aagatgtaga taaggctgtt aaagaaattg tcgagtctgt aactctattg agaaagatgt cccctctttg ggattctatt acagacacaa aggttaaaga tgaggagtca ctcaaatgga cgaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa