CrpaA.00593.a

Choline-phosphate cytidylyltransferase

CENTER ID: CrpaA.00593.a
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
CrpaA.00593.a.A1.GE26441 Full length( CrpaA.00593.a ) 1 341
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
CrpaA.00593.a.A1.PS00341 Full length( CrpaA.00593.a ) 1 341

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd8_1150
RefSeq: XP_627022.1
UniProt: Q5CW97

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
LKQSCKSKPK VIKGICKKNL CKKNSEILTE DSSENGSFID DEKSDIFNKN TLADGQENNK ECDTNRKIRI YADGVYDLLH LGHMRQLEQA KKMYPNTHLI VGVASDEETH RLKGRTVQTL QERTETLRHV KWVDEIISPC PWVIDEKFVE KHKIDFVAHD DVPYVAKQKK RDEEDSSNTD CNSSEEKPVI SYDQDDIYGW LKRCGKFKAT QRTKGVSTTD LVVRILQNYE EYIDRSLQRG VTPDELNIGY MKANQIQMKK GIQRWGEKVT NELTKVTLTD RPLGITFDES VDNIRNQIHK SFDAWRGVSK KYLEGFARTF DPMRSLFREN RISEGESPSF K
NT Sequence
CTGAAACAGA GTTGTAAATC TAAACCAAAA GTAATAAAGG GAATATGTAA AAAAAATTTA TGCAAGAAGA ATAGTGAAAT ACTTACCGAG GATAGTTCTG AGAATGGAAG CTTTATTGAT GATGAGAAAA GTGATATATT TAATAAAAAT ACTTTAGCTG ATGGACAGGA AAATAATAAA GAATGCGATA CGAATAGAAA AATTCGAATT TACGCTGATG GAGTGTATGA TTTACTTCAC CTAGGACACA TGAGACAACT AGAACAGGCT AAAAAGATGT ATCCAAATAC TCATTTAATA GTAGGTGTAG CAAGTGATGA AGAAACACAC AGATTAAAAG GAAGAACGGT ACAAACTTTG CAAGAAAGAA CAGAAACATT AAGACATGTA AAATGGGTGG ATGAGATCAT ATCTCCATGT CCATGGGTAA TTGATGAGAA ATTTGTTGAA AAACATAAGA TAGATTTTGT TGCGCATGAC GATGTTCCAT ATGTTGCAAA GCAGAAAAAA AGAGATGAAG AAGATTCATC AAACACAGAT TGTAATAGCA GTGAAGAAAA GCCAGTAATT TCGTACGACC AAGATGATAT TTACGGATGG CTTAAACGCT GTGGTAAATT TAAAGCTACT CAAAGAACAA AAGGTGTTTC TACAACAGAT TTAGTCGTAA GAATTCTTCA GAATTATGAG GAATATATTG ATCGATCTTT ACAGAGAGGT GTGACTCCAG ATGAATTGAA TATTGGTTAT ATGAAGGCAA ATCAAATCCA AATGAAGAAA GGTATTCAAA GATGGGGTGA GAAAGTTACT AATGAATTAA CAAAAGTGAC TCTCACTGAC AGGCCACTAG GTATTACATT CGACGAGAGT GTTGATAATA TTAGAAATCA AATTCACAAA TCTTTTGATG CTTGGAGAGG AGTTTCCAAA AAGTATCTTG AAGGCTTTGC CAGAACATTT GACCCAATGA GATCGTTATT CAGAGAAAAT CGTATTTCCG AGGGTGAATC TCCTTCCTTC AAG
Details for CrpaA.00593.a.A1.GE26441
HARVESTED ON: data unavailable
SEQUENCED ON: 5/6/2009
EXPECTED MW: 41kDa
OBSERVED MW: 14kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMLKQSCK SKPKVIKGIC KKNLCKKNSE ILTEDSSENG SFIDDEKSDI FNKNTLADGQ ENNKECDTNR KIRIYADGVY DLLHLGHMRQ LEQAKKMYPN THLIVGVASD EETHRLKGRT VQTLQERTET LRHVKWVDEI ISPCPWVIDE KFVEKHKIDF VAHDDVPYVA KQKKRDEEDS SNTDCNSSEE KPVISYDQDD IYGWLKRCGK FKATQRTKGV STTDLVVRIL QNYEEYIDRS LQRGVTPDEL NIGYMKANQI QMKKGIQRWG EKVTNELTKV TLTDRPLGIT FDESVDNIRN QIHKSFDAWR GVSKKYLEGF ARTFDPMRSL FRENRISEGE SPSFK
Validated NT Sequence
tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatgct gaaacagagt tgtaaatcta aaccaaaagt aataaaggga atatgtaaaa aaaatttatg caagaagaat agtgaaatac ttaccgagga tagttctgag aatggaagct ttattgatga tgagaaaagt gatatattta ataaaaatac tttagctgat ggacaggaaa ataataaaga atgcgatacg aatagaaaaa ttcgaattta cgctgatgga gtgtatgatt tacttcacct aggacacatg agacaactag aacaggctaa aaagatgtat ccaaatactc atttaatagt aggtgtagca agtgatgaag aaacacacag attaaaagga agaacggtac aaactttgca agaaagaaca gaaacattaa gacatgtaaa atgggtggat gagatcatat ctccatgtcc atgggtaatt gatgagaaat ttgttgaaaa acataagata gattttgttg cgcatgacga tgttccatat gttgcaaagc agaaaaaaag agatgaagaa gattcatcaa acacagattg taatagcagt gaagaaaagc cagtaatttc gtacgaccaa gatgatattt acggatggct taaacgctgt ggtaaattta aagctactca aagaacaaaa ggtgtttcta caacagattt agtcgtaaga attcttcaga attatgagga atatattgat cgatctttac agagaggtgt gactccagat gaattgaata ttggttatat gaaggcaaat caaatccaaa tgaagaaagg tattcaaaga tggggtgaga aagttactaa tgaattaaca aaagtgactc tcactgacag gccactaggt attacattcg acgagagtgt tgataatatt agaaatcaaa ttcacaaatc ttttgatgct tggagaggag tttccaaaaa gtatcttgaa ggctttgcca gaacatttga cccaatgaga tcgttattca gagaaaatcg tatttccgag ggtgaatctc cttccttcaa gtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLKQSCKSK PKVIKGICKK NLCKKNSEIL TEDSSENGSF IDDEKSDIFN KNTLADGQEN NKECDTNRKI RIYADGVYDL LHLGHMRQLE QAKKMYPNTH LIVGVASDEE THRLKGRTVQ TLQERTETLR HVKWVDEIIS PCPWVIDEKF VEKHKIDFVA HDDVPYVAKQ KKRDEEDSSN TDCNSSEEKP VISYDQDDIY GWLKRCGKFK ATQRTKGVST TDLVVRILQN YEEYIDRSLQ RGVTPDELNI GYMKANQIQM KKGIQRWGEK VTNELTKVTL TDRPLGITFD ESVDNIRNQI HKSFDAWRGV SKKYLEGFAR TFDPMRSLFR ENRISEGESP SFK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgaaacag agttgtaaat ctaaaccaaa agtaataaag ggaatatgta aaaaaaattt atgcaagaag aatagtgaaa tacttaccga ggatagttct gagaatggaa gctttattga tgatgagaaa agtgatatat ttaataaaaa tactttagct gatggacagg aaaataataa agaatgcgat acgaatagaa aaattcgaat ttacgctgat ggagtgtatg atttacttca cctaggacac atgagacaac tagaacaggc taaaaagatg tatccaaata ctcatttaat agtaggtgta gcaagtgatg aagaaacaca cagattaaaa ggaagaacgg tacaaacttt gcaagaaaga acagaaacat taagacatgt aaaatgggtg gatgagatca tatctccatg tccatgggta attgatgaga aatttgttga aaaacataag atagattttg ttgcgcatga cgatgttcca tatgttgcaa agcagaaaaa aagagatgaa gaagattcat caaacacaga ttgtaatagc agtgaagaaa agccagtaat ttcgtacgac caagatgata tttacggatg gcttaaacgc tgtggtaaat ttaaagctac tcaaagaaca aaaggtgttt ctacaacaga tttagtcgta agaattcttc agaattatga ggaatatatt gatcgatctt tacagagagg tgtgactcca gatgaattga atattggtta tatgaaggca aatcaaatcc aaatgaagaa aggtattcaa agatggggtg agaaagttac taatgaatta acaaaagtga ctctcactga caggccacta ggtattacat tcgacgagag tgttgataat attagaaatc aaattcacaa atcttttgat gcttggagag gagtttccaa aaagtatctt gaaggctttg ccagaacatt tgacccaatg agatcgttat tcagagaaaa tcgtatttcc gagggtgaat ctccttcctt caagaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for CrpaA.00593.a.A1.PS00341
PURIFICATION DATe: 9/2/2009
CONCENTRATION: 23.99mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMLKQSCK SKPKVIKGIC KKNLCKKNSE ILTEDSSENG SFIDDEKSDI FNKNTLADGQ ENNKECDTNR KIRIYADGVY DLLHLGHMRQ LEQAKKMYPN THLIVGVASD EETHRLKGRT VQTLQERTET LRHVKWVDEI ISPCPWVIDE KFVEKHKIDF VAHDDVPYVA KQKKRDEEDS SNTDCNSSEE KPVISYDQDD IYGWLKRCGK FKATQRTKGV STTDLVVRIL QNYEEYIDRS LQRGVTPDEL NIGYMKANQI QMKKGIQRWG EKVTNELTKV TLTDRPLGIT FDESVDNIRN QIHKSFDAWR GVSKKYLEGF ARTFDPMRSL FRENRISEGE SPSFK
Validated NT Sequence
tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatgct gaaacagagt tgtaaatcta aaccaaaagt aataaaggga atatgtaaaa aaaatttatg caagaagaat agtgaaatac ttaccgagga tagttctgag aatggaagct ttattgatga tgagaaaagt gatatattta ataaaaatac tttagctgat ggacaggaaa ataataaaga atgcgatacg aatagaaaaa ttcgaattta cgctgatgga gtgtatgatt tacttcacct aggacacatg agacaactag aacaggctaa aaagatgtat ccaaatactc atttaatagt aggtgtagca agtgatgaag aaacacacag attaaaagga agaacggtac aaactttgca agaaagaaca gaaacattaa gacatgtaaa atgggtggat gagatcatat ctccatgtcc atgggtaatt gatgagaaat ttgttgaaaa acataagata gattttgttg cgcatgacga tgttccatat gttgcaaagc agaaaaaaag agatgaagaa gattcatcaa acacagattg taatagcagt gaagaaaagc cagtaatttc gtacgaccaa gatgatattt acggatggct taaacgctgt ggtaaattta aagctactca aagaacaaaa ggtgtttcta caacagattt agtcgtaaga attcttcaga attatgagga atatattgat cgatctttac agagaggtgt gactccagat gaattgaata ttggttatat gaaggcaaat caaatccaaa tgaagaaagg tattcaaaga tggggtgaga aagttactaa tgaattaaca aaagtgactc tcactgacag gccactaggt attacattcg acgagagtgt tgataatatt agaaatcaaa ttcacaaatc ttttgatgct tggagaggag tttccaaaaa gtatcttgaa ggctttgcca gaacatttga cccaatgaga tcgttattca gagaaaatcg tatttccgag ggtgaatctc cttccttcaa gtaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLKQSCKSK PKVIKGICKK NLCKKNSEIL TEDSSENGSF IDDEKSDIFN KNTLADGQEN NKECDTNRKI RIYADGVYDL LHLGHMRQLE QAKKMYPNTH LIVGVASDEE THRLKGRTVQ TLQERTETLR HVKWVDEIIS PCPWVIDEKF VEKHKIDFVA HDDVPYVAKQ KKRDEEDSSN TDCNSSEEKP VISYDQDDIY GWLKRCGKFK ATQRTKGVST TDLVVRILQN YEEYIDRSLQ RGVTPDELNI GYMKANQIQM KKGIQRWGEK VTNELTKVTL TDRPLGITFD ESVDNIRNQI HKSFDAWRGV SKKYLEGFAR TFDPMRSLFR ENRISEGESP SFK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCTGAAACAG AGTTGTAAAT CTAAACCAAA AGTAATAAAG GGAATATGTA AAAAAAATTT ATGCAAGAAG AATAGTGAAA TACTTACCGA GGATAGTTCT GAGAATGGAA GCTTTATTGA TGATGAGAAA AGTGATATAT TTAATAAAAA TACTTTAGCT GATGGACAGG AAAATAATAA AGAATGCGAT ACGAATAGAA AAATTCGAAT TTACGCTGAT GGAGTGTATG ATTTACTTCA CCTAGGACAC ATGAGACAAC TAGAACAGGC TAAAAAGATG TATCCAAATA CTCATTTAAT AGTAGGTGTA GCAAGTGATG AAGAAACACA CAGATTAAAA GGAAGAACGG TACAAACTTT GCAAGAAAGA ACAGAAACAT TAAGACATGT AAAATGGGTG GATGAGATCA TATCTCCATG TCCATGGGTA ATTGATGAGA AATTTGTTGA AAAACATAAG ATAGATTTTG TTGCGCATGA CGATGTTCCA TATGTTGCAA AGCAGAAAAA AAGAGATGAA GAAGATTCAT CAAACACAGA TTGTAATAGC AGTGAAGAAA AGCCAGTAAT TTCGTACGAC CAAGATGATA TTTACGGATG GCTTAAACGC TGTGGTAAAT TTAAAGCTAC TCAAAGAACA AAAGGTGTTT CTACAACAGA TTTAGTCGTA AGAATTCTTC AGAATTATGA GGAATATATT GATCGATCTT TACAGAGAGG TGTGACTCCA GATGAATTGA ATATTGGTTA TATGAAGGCA AATCAAATCC AAATGAAGAA AGGTATTCAA AGATGGGGTG AGAAAGTTAC TAATGAATTA ACAAAAGTGA CTCTCACTGA CAGGCCACTA GGTATTACAT TCGACGAGAG TGTTGATAAT ATTAGAAATC AAATTCACAA ATCTTTTGAT GCTTGGAGAG GAGTTTCCAA AAAGTATCTT GAAGGCTTTG CCAGAACATT TGACCCAATG AGATCGTTAT TCAGAGAAAA TCGTATTTCC GAGGGTGAAT CTCCTTCCTT CAAGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA