CrpaA.00439.a

Casein kinase II, alpha subunit, putative

CENTER ID:	CrpaA.00439.a
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.00439.a.A1.GE39370	Full length( CrpaA.00439.a )		1	341

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd6_620
OrthoMCL:	OG5_126901
RefSeq:	XP_001388302.1
UniProt:	A3FPS3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSNNSNSANK WPLPRLYSDV NIYKPREYWD YESFQIEYSN PNNYEIIKKV GRGKYSEVFE GINVITNEKV IIKVLRPVKR KKIKREIKIL YNLHGGPNIV KLLDIIKDPS SGTISLVFEC LDNVDFKSLF PTFNDNDIRF YIYQILKALD FCHSQGIMHR DVKPHNVIID PKKKELRLID WGLAEFYHPR QDYNVRVASR YYKGPELLID FQYYDYSLDI WSLGCVLAGL IFKRDPFFCG VDNNDQLVKI ARVLGTDGLF EYLAKYKKQL EPYFDDLLGR HTRKPWSKFI NAENQHLVNP VVLDLLDKML VYDHAERILP REALAHPYFE KIHQEEQKGS S

NT Sequence

ATGAGTAACA ATAGTAATTC AGCTAATAAA TGGCCACTTC CAAGATTATA TTCAGATGTT AATATATATA AACCTCGTGA ATATTGGGAT TATGAAAGCT TTCAAATTGA ATATAGTAAC CCAAATAACT ATGAAATAAT TAAAAAAGTA GGTAGAGGAA AATACAGTGA AGTATTTGAA GGAATTAATG TGATTACAAA TGAGAAGGTA ATAATTAAAG TACTTAGACC GGTTAAAAGA AAGAAAATTA AGCGTGAAAT AAAAATATTA TATAATTTAC ATGGTGGTCC AAATATTGTA AAATTGTTGG ACATAATAAA AGATCCAAGC AGTGGTACAA TAAGTCTTGT ATTTGAATGC CTTGATAATG TTGATTTTAA AAGCTTATTT CCAACATTTA ATGATAATGA TATAAGATTT TATATATATC AAATTCTAAA AGCATTAGAT TTTTGTCATT CACAAGGTAT TATGCACCGT GATGTTAAAC CACATAATGT AATTATTGAT CCAAAAAAGA AAGAATTAAG ATTAATTGAT TGGGGTTTAG CAGAATTTTA TCATCCTAGA CAAGATTATA ACGTAAGAGT TGCTAGCAGA TACTATAAAG GACCAGAATT ACTTATTGAT TTTCAATATT ATGATTACTC TTTGGATATC TGGAGTTTAG GATGCGTTTT AGCTGGATTA ATTTTTAAGA GGGACCCATT CTTTTGTGGA GTTGATAACA ATGATCAACT TGTTAAAATT GCTAGGGTTT TAGGTACTGA TGGTTTATTT GAATATCTTG CTAAATATAA AAAACAATTG GAACCTTATT TTGATGATTT ACTTGGCAGA CATACTAGAA AACCATGGAG CAAATTTATC AATGCTGAAA ATCAACATCT TGTTAATCCT GTTGTTTTAG ATTTACTTGA TAAAATGCTA GTTTATGATC ATGCTGAGAG AATTCTTCCA AGAGAAGCTT TGGCTCATCC TTACTTTGAA AAAATTCATC AGGAAGAGCA GAAAGGATCA TCT

Details for CrpaA.00439.a.A1.GE39370

HARVESTED ON:	8/5/2015
SEQUENCED ON:	6/26/2015
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSNNSNSAN KWPLPRLYSD VNIYKPREYW DYESFQIEYS NPNNYEIIKK VGRGKYSEVF EGINVITNEK VIIKVLRPVK RKKIKREIKI LYNLHGGPNI VKLLDIIKDP SSGTISLVFE CLDNVDFKSL FPTFNDNDIR FYIYQILKAL DFCHSQGIMH RDVKPHNVII DPKKKELRLI DWGLAEFYHP RQDYNVRVAS RYYKGPELLI DFQYYDYSLD IWSLGCVLAG LIFKRDPFFC GVDNNDQLVK IARVLGTDGL FEYLAKYKKQ LEPYFDDLLG RHTRKPWSKF INAENQHLVN PVVLDLLDKM LVYDHAERIL PREALAHPYF EKIHQEEQKG SS

Validated NT Sequence

attaagatga tcctttctgc tcttcctgat gaattttttc aaagtaagga tgagccaaag cttctcttgg aagaattctc tcagcatgat cataaactag cattttatca agtaaatcta aaacaacagg attaacaaga tgttgatttt cagcattgat aaatttgctc catggttttc tagtatgtct gccaagtaaa tcatcaaaat aaggttccaa ttgtttttta tatttagcaa gatattcaaa taaaccatca gtacctaaaa ccctagcaat tttaacaagt tgatcattgt tatcaactcc acaaaagaat gggtccctct taaaaattaa tccagctaaa acgcatccta aactccagat atccaaagag taatcataat attgaaaatc aataagtaat tctggtcctt tatagtatct gctagcaact cttacgttat aatcttgtct aggatgataa aattctgcta aaccccaatc aattaatctt aattctttct tttttggatc aataattaca ttatgtggtt taacatcacg gtgcataata ccttgtgaat gacaaaaatc taatgctttt agaatttgat atatataaaa tcttatatca ttatcattaa atgttggaaa taagctttta aaatcaacat tatcaaggca ttcaaataca agacttattg taccactgct tggatctttt attatgtcca acaattttac aatatttgga ccaccatgta aattatataa tatttttatt tcacgcttaa ttttctttct tttaaccggt ctaagtactt taattattac cttctcattt gtaatcacat taattccttc aaatacttca ctgtattttc ctctacctac ttttttaatt atttcatagt tatttgggtt actatattca atttgaaagc tttcataatc ccaatattca cgaggtttat atatattaac atctgaatat aatcttggaa gtggccattt attagctgaa ttactattgt tactcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtaacaat agtaattcag ctaataaatg gccacttcca agattatatt cagatgttaa tatatataaa cctcgtgaat attgggatta tgaaagcttt caaattgaat atagtaaccc aaataactat gaaataatta aaaaagtagg tagaggaaaa tacagtgaag tatttgaagg aattaatgtg attacaaatg agaaggtaat aattaaagta cttagaccgg ttaaaagaaa gaaaattaag cgtgaaataa aaatattata taatttacat ggtggtccaa atattgtaaa attgttggac ataataaaag atccaagcag tggtacaata agtcttgtat ttgaatgcct tgataatgtt gattttaaaa gcttatttcc aacatttaat gataatgata taagatttta tatatatcaa attctaaaag cattagattt ttgtcattca caaggtatta tgcaccgtga tgttaaacca cataatgtaa ttattgatcc aaaaaagaaa gaattaagat taattgattg gggtttagca gaattttatc atcctagaca agattataac gtaagagttg ctagcagata ctataaagga ccagaattac ttattgattt tcaatattat gattactctt tggatatctg gagtttagga tgcgttttag ctggattaat ttttaagagg gacccattct tttgtggagt tgataacaat gatcaacttg ttaaaattgc tagggtttta ggtactgatg gtttatttga atatcttgct aaatataaaa aacaattgga accttatttt gatgatttac ttggcagaca tactagaaaa ccatggagca aatttatcaa tgctgaaaat caacatcttg ttaatcctgt tgttttagat ttacttgata aaatgctagt ttatgatcat gctgagagaa ttcttccaag agaagctttg gctcatcctt actttgaaaa aattcatcag gaagagcaga aaggatcatc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa