SSGCID: Target Page

CrpaA.00409.a

proteasome subunit beta type 7, putative

CENTER ID:	CrpaA.00409.a
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.00409.a.B1.GE35485	full length		1	279

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd3_2530
RefSeq:	XP_626814.1
UniProt:	Q5CUK0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNAYLNAYFS EKSTNGFLFE NNIATAPCLS DSTELKTVKT GTTIVGVACN DCVVLGADTR ATNGPIVADK DCEKIHRLSD NIFAAGAGTA ADLDHVTSLI EGNLELQKLQ MNRKPRVAHA VSMLSDHLYK YQGYIGAHLI VAGSDSTGNF VFQVSANGCI MQLPFTSMGS GSLCARSILE ARYRDGLTES ECVELVSDAI RAGIYNDLYS GSNVNILIIK NNYVKHFRHF DTKASERIYR QPKPISFPVG TTPIIAEKTE DLSSFVVEVS EVQIDENMD

NT Sequence

atgaatgcct acttaaatgc ttatttttct gaaaaatcaa caaatgggtt cttattcgaa aataatattg caactgctcc ctgcctatct gactcaactg agcttaaaac agttaaaact ggtactacga ttgtaggagt tgcttgcaac gattgtgttg ttcttggtgc agatactaga gcaacgaatg gtcctattgt tgcagataaa gattgcgaaa agatacaccg actttcagat aacatttttg ctgctggggc aggaactgct gctgaccttg atcatgtaac ttctcttatt gaggggaatt tagaattgca gaaacttcaa atgaatcgaa aacccagagt agcgcatgcg gtttctatgc ttagcgacca cttatataaa taccaaggct atattggtgc ccatttgata gttgcaggct ccgattctac aggaaatttt gtattccaag tttcagcaaa tggttgtatc atgcagttgc cgtttacatc tatggggagt ggttctctat gcgcaaggtc gatattagaa gcaaggtata gggatggtct aacagagagt gaatgtgtag aattagtttc agatgcgatt agggcaggga tttataatga tctctatagt ggaagtaacg taaatattct tattatcaaa aacaattatg ttaaacattt cagacatttt gatactaagg ccagtgaaag aatctaccga cagccaaagc ccatttcatt tcccgttggt acaactccaa ttattgcaga aaagacggaa gatttgagct catttgttgt ggaggtatct gaagtacaaa tcgatgaaaa tatggattaa

Details for CrpaA.00409.a.B1.GE35485

HARVESTED ON:	6/26/2012
SEQUENCED ON:	6/29/2012
EXPECTED MW:	31kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN AYLNAYFSEK STNGFLFENN IATAPCLSDS TELKTVKTGT TIVGVACNDC VVLGADTRAT NGPIVADKDC EKIHRLSDNI FAAGAGTAAD LDHVTSLIEG NLELQKLQMN RKPRVAHAVS MLSDHLYKYQ GYIGAHLIVA GSDSTGNFVF QVSANGCIMQ LPFTSMGSGS LCARSILEAR YRDGLTESEC VELVSDAIRA GIYNDLYSGS NVNILIIKNN YVKHFRHFDT KASERIYRQP KPISFPVGTT PIIAEKTEDL SSFVVEVSEX QIDENMD

Validated NT Sequence

ttnnnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatgaatgcc tacttaaatg cttatttttc tgaaaaatca acaaatgggt tcttattcga aaataatatt gcaactgctc cctgcctatc tgactcaact gagcttaaaa cagttaaaac tggtactacg attgtaggag ttgcttgcaa cgattgtgtt gttcttggtg cagatactag agcaacgaat ggtcctattg ttgcagataa agattgcgaa aagatacacc gactttcaga taacattttt gctgctgggg caggaactgc tgctgacctt gatcatgtaa cttctcttat tgaggggaat ttagaattgc agaaacttca aatgaatcga aaacccagag tagcgcatgc ggtttctatg cttagcgacc acttatataa ataccaaggc tatattggtg cccatttgat agttgcaggc tccgattcta caggaaattt tgtattccaa gtttcagcaa atggttgtat catgcagttg ccgtttacat ctatggggag tggttctcta tgcgcaaggt cgatattaga agcaaggtat agggatggtc taacagagag tgaatgtgta gaattagttt cagatgcgat tagggcaggg atttataatg atctctatag tggaagtaac gtaaatattc ttattatcaa aaacaattat gttaaacatt tcagacattt tgatactaag gccagtgaaa gaatctaccg acagccaaag cccatttcat ttcccgttgg tacaactccn attattgcag aaaagacgga agatttgagc tcatttgttg tggaggtatc tgaantacaa atcgatgaaa atatggatta agtggagtaa nanaggatcc ggctgcnann nnncccnaaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg cctacttaaa tgcttatttt tctgaaaaat caacaaatgg gttcttattc gaaaataata ttgcaactgc tccctgccta tctgactcaa ctgagcttaa aacagttaaa actggtacta cgattgtagg agttgcttgc aacgattgtg ttgttcttgg tgcagatact agagcaacga atggtcctat tgttgcagat aaagattgcg aaaagataca ccgactttca gataacattt ttgctgctgg ggcaggaact gctgctgacc ttgatcatgt aacttctctt attgagggga atttagaatt gcagaaactt caaatgaatc gaaaacccag agtagcgcat gcggtttcta tgcttagcga ccacttatat aaataccaag gctatattgg tgcccatttg atagttgcag gctccgattc tacaggaaat tttgtattcc aagtttcagc aaatggttgt atcatgcagt tgccgtttac atctatgggg agtggttctc tatgcgcaag gtcgatatta gaagcaaggt atagggatgg tctaacagag agtgaatgtg tagaattagt ttcagatgcg attagggcag ggatttataa tgatctctat agtggaagta acgtaaatat tcttattatc aaaaacaatt atgttaaaca tttcagacat tttgatacta aggccagtga aagaatctac cgacagccaa agcccatttc atttcccgtt ggtacaactc caattattgc agaaaagacg gaagatttga gctcatttgt tgtggaggta tctgaagtac aaatcgatga aaatatggat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat