SSGCID: Target Page

CrpaA.00346.a

Putative uncharacterized protein

CENTER ID:	CrpaA.00346.a
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.00346.a.B1.GE26959	Full length( CrpaA.00346.a )		1	383

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd4_1400
RefSeq:	XP_625736.1
UniProt:	Q5CQB2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTEYRVGFA SKEDIIEKST YGECEDRTKS LSFSSDVNSI TYDEASSSSE EYPEYSLLRT QSSLAPPIEA SAIVDVEEAW KALSKELEES HSKPTIRTLG TVFVTREEVG TKDSIEYRLF YKNEDGYKIS PWHDVPLWFS ETPLLYNMII EIPKLTNKKF EINTKEEYTP LYQDRKLERL RTYPGPIPWN YGAFPQTWED PNKKGDENVD FSHGDNDPLD AVEIGVGPLP RGTIIQVKIL GCLALIDDDE LDWKVVCIRV CDPHASQLND ITDVEKYFPG TIDRIRRWFG LYKAVENKDV AKVNMYGHFG EPQSAEFAHS VILETHHSYL RLIRGEAMNS SSLWIPKSLS NNGETELCHT VCSTNSTATV SSNDIGGLSV NDD

NT Sequence

ATGAGTACTG AATACAGAGT GGGTTTTGCC TCGAAAGAGG ATATTATAGA AAAAAGTACT TATGGAGAAT GTGAGGATAG GACAAAAAGC TTGAGCTTCA GTAGTGATGT GAATAGTATT ACTTATGATG AAGCATCTTC ATCAAGTGAG GAATATCCAG AATATAGTTT ATTACGCACT CAAAGTTCTT TAGCTCCACC AATTGAGGCT TCTGCAATAG TAGATGTAGA AGAAGCATGG AAAGCCTTAT CTAAAGAATT AGAAGAAAGT CATTCTAAAC CAACAATTCG TACTCTTGGG ACAGTTTTTG TAACAAGAGA AGAGGTTGGA ACAAAAGATT CAATTGAATA TAGATTATTT TATAAGAATG AAGATGGCTA TAAGATTTCT CCATGGCATG ATGTACCATT ATGGTTTTCA GAAACACCTC TTTTATATAA TATGATAATT GAGATTCCAA AATTAACAAA TAAAAAATTT GAGATAAATA CAAAAGAGGA ATATACACCA TTATATCAGG ATCGAAAGCT GGAAAGACTT AGAACCTATC CAGGTCCAAT TCCTTGGAAC TATGGTGCTT TTCCACAAAC ATGGGAAGAT CCAAATAAGA AGGGAGATGA GAATGTTGAT TTTAGTCATG GTGATAACGA TCCACTGGAC GCTGTTGAAA TTGGAGTTGG ACCTCTTCCA CGTGGAACAA TTATACAGGT AAAAATTTTG GGTTGCCTTG CTTTAATTGA TGATGATGAA TTGGATTGGA AAGTTGTATG TATTCGTGTT TGTGACCCAC ATGCATCACA GCTAAATGAT ATTACTGATG TTGAGAAATA TTTCCCTGGT ACTATAGATA GAATTAGGAG ATGGTTTGGG CTTTATAAAG CAGTTGAGAA TAAAGATGTT GCAAAGGTTA ATATGTATGG ACATTTTGGA GAGCCTCAAT CAGCAGAGTT TGCTCATTCA GTAATTTTAG AAACTCATCA TTCGTACTTA CGTCTTATTA GAGGAGAGGC AATGAATTCA TCATCATTAT GGATACCAAA AAGTCTTTCG AATAATGGAG AAACAGAATT GTGTCATACA GTATGTTCCA CAAATAGCAC TGCTACAGTA TCTAGCAATG ATATTGGAGG GCTTTCTGTA AATGATGAT

Details for CrpaA.00346.a.B1.GE26959

HARVESTED ON:	6/18/2009
SEQUENCED ON:	7/15/2009
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

Validated NT Sequence

ccaatatcat tgctagatac tgtagcagtg ctatttgtgg aacatactgt atgacacaat tctgtttctc cattattcga aagacttttt ggtatccata atgatgatga attcattgcc tctcctctaa taagacgtaa gtacgaatga tgagtttcta aaattactga atgagcaaac tctgctgatt gaggctctcc aaaatgtcca tacatattaa cctttgcaac atctttattc tcaactgctt tataaagccc aaaccatctc ctaattctat ctatagtacc agggaaatat ttctcaacat cagtaatatc atttagctgt gatgcatgtg ggtcacaaac acgaatacat acaactttcc aatccaattc atcatcatca attaaagcaa ggcaacccaa aatttttacc tgtataattg ttccacgtgg aagaggtcca actccaattt caacagcgtc cagtggatcg ttatcaccat gactaaaatc aacattctca tctcccttct tatttggatc ttcccatgtt tgtggaaaag caccatagtt ccaaggaatt ggacctggat aggttctaag tctttccagc tttcgatcct gatataatgg tgtatattcc tcttttgtat ttatctcaaa ttttttattt gttaattttg gaatctcaat tatcatatta tataaaagag gtgtttctga aaaccataat ggtacatcat gccatggaga aatcttatag ccatcttcat tcttataaaa taatctatat tcaattgaat cttttgttcc aacctcttct cttgttacaa aaactgtccc aagagtacga attgttggtt tagaatgact ttcttctaat tctttagata aggctttcca tgcttcttct acatctacta ttgcagaagc ctcaattggt ggagctaaag aactttgagt gcgtaataaa ctatattctg gatattcctc acttgatgaa gatgcttcat cataagtaat actattcaca tcactactga agctcaagct ttttgtccta tcctcacatt ctccataagt acttttttct ataatatcct ctttcgaggc aaaacccact ctgtattcag tactcatatg gtggtggtgg tggtgagcca

Expected Protein Sequence

MAHHHHHHMS TEYRVGFASK EDIIEKSTYG ECEDRTKSLS FSSDVNSITY DEASSSSEEY PEYSLLRTQS SLAPPIEASA IVDVEEAWKA LSKELEESHS KPTIRTLGTV FVTREEVGTK DSIEYRLFYK NEDGYKISPW HDVPLWFSET PLLYNMIIEI PKLTNKKFEI NTKEEYTPLY QDRKLERLRT YPGPIPWNYG AFPQTWEDPN KKGDENVDFS HGDNDPLDAV EIGVGPLPRG TIIQVKILGC LALIDDDELD WKVVCIRVCD PHASQLNDIT DVEKYFPGTI DRIRRWFGLY KAVENKDVAK VNMYGHFGEP QSAEFAHSVI LETHHSYLRL IRGEAMNSSS LWIPKSLSNN GETELCHTVC STNSTATVSS NDIGGLSVND D

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta ctgaatacag agtgggtttt gcctcgaaag aggatattat agaaaaaagt acttatggag aatgtgagga taggacaaaa agcttgagct tcagtagtga tgtgaatagt attacttatg atgaagcatc ttcatcaagt gaggaatatc cagaatatag tttattacgc actcaaagtt ctttagctcc accaattgag gcttctgcaa tagtagatgt agaagaagca tggaaagcct tatctaaaga attagaagaa agtcattcta aaccaacaat tcgtactctt gggacagttt ttgtaacaag agaagaggtt ggaacaaaag attcaattga atatagatta ttttataaga atgaagatgg ctataagatt tctccatggc atgatgtacc attatggttt tcagaaacac ctcttttata taatatgata attgagattc caaaattaac aaataaaaaa tttgagataa atacaaaaga ggaatataca ccattatatc aggatcgaaa gctggaaaga cttagaacct atccaggtcc aattccttgg aactatggtg cttttccaca aacatgggaa gatccaaata agaagggaga tgagaatgtt gattttagtc atggtgataa cgatccactg gacgctgttg aaattggagt tggacctctt ccacgtggaa caattataca ggtaaaaatt ttgggttgcc ttgctttaat tgatgatgat gaattggatt ggaaagttgt atgtattcgt gtttgtgacc cacatgcatc acagctaaat gatattactg atgttgagaa atatttccct ggtactatag atagaattag gagatggttt gggctttata aagcagttga gaataaagat gttgcaaagg ttaatatgta tggacatttt ggagagcctc aatcagcaga gtttgctcat tcagtaattt tagaaactca tcattcgtac ttacgtctta ttagaggaga ggcaatgaat tcatcatcat tatggatacc aaaaagtctt tcgaataatg gagaaacaga attgtgtcat acagtatgtt ccacaaatag cactgctaca gtatctagca atgatattgg agggctttct gtaaatgatg attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat