SSGCID: Target Page

CrpaA.00284.a

Ankyrin-related protein, putative

CENTER ID:	CrpaA.00284.a
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.00284.a.B1.GE26958	Full length( CrpaA.00284.a )		1	324

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd6_4590
OrthoMCL:	OG5_142636
RefSeq:	XP_001388349.1
UniProt:	A3FPX0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDNQQSELES TKYSESSSNT KALASLKGSL PEDCYYSADE DFQLENEEAV NTNLIAPSGR FSNSNRSNGL KTLGNSNQVY KQDNIRSQKL PSFGFENRFQ DNIKGKGRTS GSIYRLDKTE NEKDTCQVCE LDTNSSLSVL SNSEDNISEF SILSDTDVFS LPAERQNSLI LKASKIGNLQ LVRQLIASPK FNANATNEKG FSSLHWASLK GHSQVVEELL NANAEPNTKN VMLCTPLHFS ASNGFDEIVN KLLNAGANPN AVSALGDTPI FSAAFMGHEN TVKTLLKNGA DPRYKNKQGL TPKDAAESQG HSEIVKILEG ACMQ

NT Sequence

ATGGATAATC AACAGTCGGA GCTAGAAAGC ACTAAATACA GTGAAAGCTC ATCCAATACA AAAGCTTTAG CTAGCCTCAA AGGAAGCTTA CCAGAGGACT GTTATTATAG TGCAGATGAG GATTTCCAAC TTGAAAATGA GGAGGCGGTG AATACAAATC TGATAGCTCC AAGTGGAAGA TTTTCAAACT CGAACAGGAG CAATGGATTA AAGACATTAG GAAATAGCAA CCAAGTTTAC AAACAAGATA ATATTCGAAG CCAAAAACTG CCTTCATTTG GGTTTGAGAA CAGATTTCAG GATAATATTA AAGGAAAGGG AAGGACTTCT GGTAGTATTT ATCGGTTAGA TAAGACTGAG AATGAAAAGG ATACTTGCCA AGTTTGCGAG CTAGATACAA ACTCAAGCTT GTCAGTATTG TCAAATTCTG AAGATAATAT CTCAGAATTT AGTATATTAA GCGATACTGA TGTATTTTCA CTTCCTGCTG AAAGACAAAA TTCATTAATA CTTAAAGCTT CTAAGATTGG GAATCTTCAA TTAGTTCGTC AACTAATTGC ATCTCCCAAA TTCAATGCAA ATGCTACAAA TGAAAAGGGG TTTTCTTCGC TTCACTGGGC TTCTCTTAAA GGGCATAGCC AAGTAGTTGA AGAATTGTTA AATGCCAATG CTGAACCCAA TACTAAGAAT GTTATGCTCT GTACTCCTTT GCACTTTTCA GCAAGCAATG GATTTGATGA GATAGTAAAC AAACTATTAA ATGCAGGAGC AAATCCAAAT GCTGTGAGCG CTCTTGGAGA TACACCTATA TTTTCTGCCG CTTTTATGGG TCATGAAAAT ACTGTAAAAA CATTACTTAA GAATGGTGCA GACCCAAGAT ACAAAAATAA GCAAGGCTTA ACTCCTAAGG ACGCAGCTGA ATCACAAGGT CACTCAGAAA TCGTAAAAAT CCTGGAAGGC GCGTGCATGC AA

Details for CrpaA.00284.a.B1.GE26958

HARVESTED ON:	6/18/2009
SEQUENCED ON:	7/15/2009
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	28

Validated AA Sequence

MLCTPLHFSA SNGFDEIVNK LLNAGANPNA VSALGDTPIF SAAFMGHENT VKTLLKNGAD PRYKNKQGLT PKDAAESQGH SEIVKILEGA CMQ

Validated NT Sequence

tattgcatgc acgcgccttc caggattttt acgatttctg agtgaccttg tgattcagct gcgtccttag gagttaagcc ttgcttattt ttgtatcttg ggtctgcacc attcttaagt aatgttttta cagtattttc atgacccata aaagcggcag aaaatatagg tgtatctcca agagcgctca cagcatttgg atttgctcct gcatttaata gtttgtttac tatctcatca aatccattgc ttgctgaaaa gtgcaaagga gtacagagca taacattctt agtattgggt tcagcattgg catttaacaa ttcttcaact acttggctat gccctttaag agaagcccag tgaagcgaag aaaacccctt ttcatttgta gcatttgcat tgaatttggg agatgcaatt agttgacgaa ctaattgaag attcccaatc ttagaagctt taagtattaa tgaattttgt ctttcagcag gaagtgaaaa tacatcagta tcgcttaata tactaaattc tgagatatta tcttcagaat ttgacaatac tgacaagctt gagtttgtat ctagctcgca aacttggcaa gtatcctttt cattctcagt cttatctaac cgataaatac taccagaagt ccttcccttt cctttaatat tatcctgaaa tctgttctca aacccaaatg aaggcagttt ttggcttcga atattatctt gtttgtaaac ttggttgcta tttcctaatg tctttaatcc attgctcctg ttcgagtttg aaaatcttcc acttggagct atcagatttg tattcaccgc ctcctcattt tcaagttgga aatcctcatc tgcactataa taacagtcct ctggtaagct tcctttgagg ctagctaaag cttttgtatt ggatgagctt tcactgtatt tagtgctttc tagctccgac tgttgattat cccatatggt ggtggnggtg gtnagccang gtannnctcc ttct

Expected Protein Sequence

MAHHHHHHMD NQQSELESTK YSESSSNTKA LASLKGSLPE DCYYSADEDF QLENEEAVNT NLIAPSGRFS NSNRSNGLKT LGNSNQVYKQ DNIRSQKLPS FGFENRFQDN IKGKGRTSGS IYRLDKTENE KDTCQVCELD TNSSLSVLSN SEDNISEFSI LSDTDVFSLP AERQNSLILK ASKIGNLQLV RQLIASPKFN ANATNEKGFS SLHWASLKGH SQVVEELLNA NAEPNTKNVM LCTPLHFSAS NGFDEIVNKL LNAGANPNAV SALGDTPIFS AAFMGHENTV KTLLKNGADP RYKNKQGLTP KDAAESQGHS EIVKILEGAC MQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggata atcaacagtc ggagctagaa agcactaaat acagtgaaag ctcatccaat acaaaagctt tagctagcct caaaggaagc ttaccagagg actgttatta tagtgcagat gaggatttcc aacttgaaaa tgaggaggcg gtgaatacaa atctgatagc tccaagtgga agattttcaa actcgaacag gagcaatgga ttaaagacat taggaaatag caaccaagtt tacaaacaag ataatattcg aagccaaaaa ctgccttcat ttgggtttga gaacagattt caggataata ttaaaggaaa gggaaggact tctggtagta tttatcggtt agataagact gagaatgaaa aggatacttg ccaagtttgc gagctagata caaactcaag cttgtcagta ttgtcaaatt ctgaagataa tatctcagaa tttagtatat taagcgatac tgatgtattt tcacttcctg ctgaaagaca aaattcatta atacttaaag cttctaagat tgggaatctt caattagttc gtcaactaat tgcatctccc aaattcaatg caaatgctac aaatgaaaag gggttttctt cgcttcactg ggcttctctt aaagggcata gccaagtagt tgaagaattg ttaaatgcca atgctgaacc caatactaag aatgttatgc tctgtactcc tttgcacttt tcagcaagca atggatttga tgagatagta aacaaactat taaatgcagg agcaaatcca aatgctgtga gcgctcttgg agatacacct atattttctg ccgcttttat gggtcatgaa aatactgtaa aaacattact taagaatggt gcagacccaa gatacaaaaa taagcaaggc ttaactccta aggacgcagc tgaatcacaa ggtcactcag aaatcgtaaa aatcctggaa ggcgcgtgca tgcaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat