CrpaA.00218.a

ubiquitin-conjugating enzyme, putative

CENTER ID: CrpaA.00218.a
ORGANISM: Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE: cryptosporidiosis - (diarrheal disease)
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
CrpaA.00218.a.B1.GE35484 full length 1 197
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: CryptoDB:cgd4_210
RefSeq: XP_625630.1
UniProt: Q5CQL8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
LIFYYKYLKE LRGLQVFTDE LEEDKQVGIK LIGNRNDNFL GIIRGPIGTP YEGGIFQLDI IVPKEYPYEP PKVKFITRIW HPNISSQTGA ICLDILKDAW SPALTLRTVM LSIQALLSSP EPNDPQDALV ASLYKSDYQE YIETAKSWTQ MYAKPTSKEE RIKRFLDMGF NRESIITALE RNNWDENLAL NELLFEN
NT Sequence
ttaatttttt attataaata tttaaaggaa ctcagaggcc tccaagtatt tacagacgag cttgaagaag ataagcaggt tggaataaag ctgattggta atagaaacga caactttttg ggtattatta gagggcctat aggaacacca tatgaaggtg gtatattcca acttgatatt attgtaccaa aagaatatcc atacgagcct ccaaaagtta aattcatcac aagaatttgg catccgaata tttctagtca aacaggagct atttgcttgg atattttaaa agatgcctgg agccctgctt taactcttag aacagtaatg ctttctatac aagcattact ctcttctcct gaaccaaatg atcctcaaga tgcattggtc gcatctctat acaaaagtga ttaccaagaa tatattgaaa cggccaaaag ttggacacaa atgtatgcaa agccaacatc aaaggaagaa aggattaaga gatttcttga tatgggattc aaccgtgaat ctataataac agcactagaa agaaataatt gggatgaaaa tctcgctcta aatgagctat tatttgaaaa ctaa
Details for CrpaA.00218.a.B1.GE35484
HARVESTED ON: 6/26/2012
SEQUENCED ON: 6/29/2012
EXPECTED MW: 23kDa
OBSERVED MW: 23kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MLIFYYKYLK ELRGLQVFTD ELEEDKQVGI KLIGNRNDNF LGIIRGPIGT PYEGGIFQLD IIVPKEYPYE PPKVKFITRI WHPNISSQTG AICLDILKDA WSPALTLRTV MLSIQALLSS PEPNDPQDAL VASLYKSDYQ EYIETAKSWT QMYAKPTSKE ERIKRFLDMG FNRESIITAL ERNNWDENLA LNELLFEN
Validated NT Sequence
gntagcagcc ggatcctatc ttactcactt agttttcaaa taatagctca tttagagcga gattttcatc ccaattattt ctttctagtg ctgttattat agattcacgg ttgaatccca tatcaagaaa tctcttaatc ctttcttcct ttgatgttgg ctttgcatac atttgtgtcc aacttttggc cgtttcaata tattcttggt aatcactttt gtatagagat gcgaccaatg catcttgagg atcatttggt tcaggagaag agagtaatgc ttgtatagaa agcattactg ttctaagagt taaagcaggg ctccaggcat cttttaaaat atccaagcaa atagctcctg tttgactaga aatattcgga tgccaaattc ttgtgatgaa tttaactttt ggaggctcgt atggatattc ttttggtaca ataatatcaa gttggaatat accaccttca tatggtgttc ctataggccc tctaataata cccaaaaagt tgtcgtttct attaccaatc agctttattc caacctgctt atcttcttca agctcgtctg taaatacttg gaggcctctg agttccttta aatatttata ataaaaaatt aacatatggt ggtggtggtg gtgagcc
Expected Protein Sequence
MAHHHHHHML IFYYKYLKEL RGLQVFTDEL EEDKQVGIKL IGNRNDNFLG IIRGPIGTPY EGGIFQLDII VPKEYPYEPP KVKFITRIWH PNISSQTGAI CLDILKDAWS PALTLRTVML SIQALLSSPE PNDPQDALVA SLYKSDYQEY IETAKSWTQM YAKPTSKEER IKRFLDMGFN RESIITALER NNWDENLALN ELLFEN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa ttttttatta taaatattta aaggaactca gaggcctcca agtatttaca gacgagcttg aagaagataa gcaggttgga ataaagctga ttggtaatag aaacgacaac tttttgggta ttattagagg gcctatagga acaccatatg aaggtggtat attccaactt gatattattg taccaaaaga atatccatac gagcctccaa aagttaaatt catcacaaga atttggcatc cgaatatttc tagtcaaaca ggagctattt gcttggatat tttaaaagat gcctggagcc ctgctttaac tcttagaaca gtaatgcttt ctatacaagc attactctct tctcctgaac caaatgatcc tcaagatgca ttggtcgcat ctctatacaa aagtgattac caagaatata ttgaaacggc caaaagttgg acacaaatgt atgcaaagcc aacatcaaag gaagaaagga ttaagagatt tcttgatatg ggattcaacc gtgaatctat aataacagca ctagaaagaa ataattggga tgaaaatctc gctctaaatg agctattatt tgaaaactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at