SSGCID: Target Page

CrpaA.00164.a

Likely arginyl-tRNA synthetase family protein (ArgRS); uncharacterized protein

CENTER ID:	CrpaA.00164.a
ORGANISM:	Cryptosporidium parvum Iowa II
ASSOCIATED DISEASE:	cryptosporidiosis - (diarrheal disease)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrpaA.00164.a.B1.GE41423	full length		1	616
CrpaA.00164.a.B11.GE42117	full length		1	616

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	CryptoDB:cgd5_4400
UniProt:	A3FQP6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MENPQNIVKH AFNEAIKNLF PEFDKEAIIA ISNPNFGDYQ CNSCMTIFSQ FKGKLEGIKS PRDIGMKIKD YIEKNGDLSI NFKSIEVAPQ GFITIKLSDE FIQNNLSKLQ EMDEVKVTPT MKFHCKSGRK TPKVIVDFSS PNIAKEMHVG HLRSTIIGDT TCRILEYLGF EVLRVNHVGD WGTQFGMLIE YLKEEYPTFV ENLPEISDLQ EFYKNSKKRF DTDPQFKLRS QQNVVMLQGG DQDALTAWKK ICEVSRVEFQ KIYDRLGIRL EEFGESYYND MIPGVVKMLD EKGILKLDNG AQCVYTKINE IPLMAVKSDG GYGYDSTDLA AIHHRLITLD ADWVIYTTDM GQEEHFMKLF DVAETMGWHD PSNTRLFFIG FGVIQGEDGK KFKTRSGDVV KLTELIDEGV ARAKKELVDR RNQRRSDQTN KSNTDVEVDH PNIDDSDLDL MANTIGHAAI KYFDLKQNRL TNYRFSFDRM LDPKGNTAVY LLYAYARICA IFRKVEFIQS DNSMVIKQKV SNFKLKISNN VERKLSIQLL RFPEVFDGIL NDFFPNRLAD YCYELSECFA QFYTECRIIG LEEQQLEQSR LLLCFLTKQI LLKGLDLLGV ETLEKI

NT Sequence

atggaaaatc cacaaaatat tgtcaaacat gcattcaatg aagctattaa gaacttgttt ccagagtttg ataaagaagc aataattgca atatcaaatc caaactttgg agattatcag tgtaatagtt gtatgaccat tttctcgcaa tttaaaggta aattagaagg aataaaaagc ccacgagaca ttggaatgaa gattaaagat tatattgaga aaaatggtga tttgtcaata aattttaaat ccatagaagt tgcaccacaa ggatttatta caataaagtt atcagatgaa tttattcaaa ataatttatc taaattacaa gaaatggatg aggtaaaagt aactccaaca atgaaattcc actgcaaaag tggaagaaaa actccaaaag taattgtaga tttctcatca ccaaacattg ccaaagaaat gcatgttgga cacttaagat ctacaattat tggtgataca acatgtagaa ttcttgaata tttaggattt gaagtattaa gagttaatca cgttggagat tggggtactc aatttggcat gcttattgaa tatttaaagg aagaatatcc cacttttgtt gagaatttac cagaaatatc agatttacaa gaattctata agaactcaaa gaaaaggttt gatactgatc cacaatttaa gttaaggagc caacagaatg ttgttatgtt acaagggggt gatcaagatg ctttgactgc ttggaaaaag atttgtgagg ttagccgagt tgaatttcag aaaatttacg atcgacttgg aattcgtctt gaggaatttg gtgaaagtta ttataatgat atgatccctg gggttgttaa aatgttggat gaaaagggta ttcttaagct tgataatgga gctcagtgtg tttatactaa aattaatgaa atacctttaa tggctgttaa aagtgatgga ggatatggat atgattcaac agatttagct gcaattcacc atagattaat cactttagat gctgattggg ttatctacac tactgatatg ggccaagaag aacattttat gaaacttttc gacgttgctg aaactatggg atggcatgat ccttctaata caagactatt tttcattgga tttggagtta ttcaaggaga agatggaaaa aagtttaaaa caagaagtgg tgatgttgtc aaattaactg aacttattga cgaaggtgtt gctagagcaa agaaagaatt agttgacaga agaaatcaaa gaagatctga tcaaacaaat aaatcaaata cagatgttga agttgatcat ccaaatattg atgattctga cttggattta atggcaaata ctattggtca tgcagctatt aagtactttg atttgaagca aaatagactt actaattaca gattttcatt tgatcgaatg ttggatccaa aaggtaatac tgcagtttat ttactatatg catatgcaag aatttgtgca atctttagaa aagttgaatt cattcaatct gataatagta tggtaattaa acagaaagtt tcaaacttta agcttaagat ttctaataat gttgaacgaa aattgtctat tcagttactc agattccctg aagtttttga tggaattttg aatgatttct tccccaatag acttgctgat tattgttatg aattatccga gtgttttgca caattttata ctgaatgcag aattattgga ttagaagaac agcaacttga acaaagtagg cttttacttt gtttcttaac aaaacaaatt cttttaaaag gacttgattt attgggagtt gaaactttgg agaagattta a

Details for CrpaA.00164.a.B1.GE41423

HARVESTED ON:	data unavailable
SEQUENCED ON:	4/27/2017
EXPECTED MW:	72kDa
OBSERVED MW:	15kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHME NPQNIVKHAF NEAIKNLFPE FDKEAIIAIS NPNFGDYQCN SCMTIFSQFK GKLEGIKSPR DIGMKIKDYI EKNGDLSINF KSIEVAPQGF ITIKLSDEFI QNNLSKLQEM DEVKVTPTMK FHCKSGRKTP KVIVDFSSPN IAKEMHVGHL RSTIIGDTTC RILEYLGFEV LRVNHVGDWG TQFGMLIEYL KEEYPTFVEN LPEISDLQEF YKNSKKRFDT DPQFKLRSQQ NVVMLQGGDQ DALTAWKKIC EVSRVEFQKI YDRLGIRLEE FGESYYNDMI PGVVKMLDEK GILKLDNGAQ CVYTKINEIP LMAVKSDGGY GYDSTDLAAI HHRLITLDAD WVIYTTDMGQ EEHFMKLFDV AETMGWHDPS NTRLFFIGFG VIQGEDGKKF KTRSGDVVKL TELIDEGVAR AKKELVDRRN QRRSDQTNKS NTDVEVDHPN IDDSDLDLMA NTIGHAAIKY FDLKQNRLTN YRFSFDRMLD PKGNTAVYLL YAYARICAIF RKVEFIQSDN SMVIKQKVSN FKLKISNNVE RKLSIQLLRF PEVFDGILND FFPNRLADYC YELSECFAQF YTECRIIGLE EQQLEQSRLL LCFLTKQILL KGLDLLGVET LEKI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa atccacaaaa tattgtcaaa catgcattca atgaagctat taagaacttg tttccagagt ttgataaaga agcaataatt gcaatatcaa atccaaactt tggagattat cagtgtaata gttgtatgac cattttctcg caatttaaag gtaaattaga aggaataaaa agcccacgag acattggaat gaagattaaa gattatattg agaaaaatgg tgatttgtca ataaatttta aatccataga agttgcacca caaggattta ttacaataaa gttatcagat gaatttattc aaaataattt atctaaatta caagaaatgg atgaggtaaa agtaactcca acaatgaaat tccactgcaa aagtggaaga aaaactccaa aagtaattgt agatttctca tcaccaaaca ttgccaaaga aatgcatgtt ggacacttaa gatctacaat tattggtgat acaacatgta gaattcttga atatttagga tttgaagtat taagagttaa tcacgttgga gattggggta ctcaatttgg catgcttatt gaatatttaa aggaagaata tcccactttt gttgagaatt taccagaaat atcagattta caagaattct ataagaactc aaagaaaagg tttgatactg atccacaatt taagttaagg agccaacaga atgttgttat gttacaaggg ggtgatcaag atgctttgac tgcttggaaa aagatttgtg aggttagccg agttgaattt cagaaaattt acgatcgact tggaattcgt cttgaggaat ttggtgaaag ttattataat gatatgatcc ctggggttgt taaaatgttg gatgaaaagg gtattcttaa gcttgataat ggagctcagt gtgtttatac taaaattaat gaaatacctt taatggctgt taaaagtgat ggaggatatg gatatgattc aacagattta gctgcaattc accatagatt aatcacttta gatgctgatt gggttatcta cactactgat atgggccaag aagaacattt tatgaaactt ttcgacgttg ctgaaactat gggatggcat gatccttcta atacaagact atttttcatt ggatttggag ttattcaagg agaagatgga aaaaagttta aaacaagaag tggtgatgtt gtcaaattaa ctgaacttat tgacgaaggt gttgctagag caaagaaaga attagttgac agaagaaatc aaagaagatc tgatcaaaca aataaatcaa atacagatgt tgaagttgat catccaaata ttgatgattc tgacttggat ttaatggcaa atactattgg tcatgcagct attaagtact ttgatttgaa gcaaaataga cttactaatt acagattttc atttgatcga atgttggatc caaaaggtaa tactgcagtt tatttactat atgcatatgc aagaatttgt gcaatcttta gaaaagttga attcattcaa tctgataata gtatggtaat taaacagaaa gtttcaaact ttaagcttaa gatttctaat aatgttgaac gaaaattgtc tattcagtta ctcagattcc ctgaagtttt tgatggaatt ttgaatgatt tcttccccaa tagacttgct gattattgtt atgaattatc cgagtgtttt gcacaatttt atactgaatg cagaattatt ggattagaag aacagcaact tgaacaaagt aggcttttac tttgtttctt aacaaaacaa attcttttaa aaggacttga tttattggga gttgaaactt tggagaagat ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for CrpaA.00164.a.B11.GE42117

HARVESTED ON:	1/16/2018
SEQUENCED ON:	1/17/2018
EXPECTED MW:	72kDa
OBSERVED MW:	72kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	81
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHME NPQNIVKHAF NEAIKNLFPE FDKEAIIAIS NPNFGDYQCN SCMTIFSQFK GKLEGIKSPR DIGMKIKDYI EKNGDLSINF KSIEVAPQGF ITIKLSDEFI QNNLSKLQEM DEVKVTPTMK FHCKSGRKTP KVIVDFSSPN IAKEMHVGHL RSTIIGDTTC RILEYLGFEV LRVNHVGDWG TQFGMLIEYL KEEYPTFVEN LPEISDLQEF YKNSKKRFDT DPQFKLXSQX XVVMLQGGXQ DALTAXXXIC EVSRVEXXXX XXRLXXXXEX XXESXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXNXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXLXX XXXXXTXXXG XXXXXXKLXX XXXXMGWHDX XNTXXFFIGX XVIQGEDGKK FKTRSGDVVK LTELIDEGVA RAKKELVDRR NQRRSDQTNK SNTDVEVDHP NIDDSXXDLM ANTIGHAAIK YFDLKQNRLT NYRFSFDRML DPKGNTAVYL LYAYARICAI FRKVEFIQSD NSMVIKQKVS NFKLKISNNV ERKLSIQLLR FPEVFDGILN DFFPNRLXDY CYELSECFAQ FYTECRIIGL EEQQLEQSRL LLCFLTKQIL LKGLD

Validated NT Sequence

atggctcacc accaccacca ccatatggaa aatccacaaa atattgtcaa acatgcattc aatgaagcta ttaagaactt gtttccagag tttgataaag aagcaataat tgcaatatca aatccaaact ttggagatta tcagtgtaat agttgtatga ccattttctc gcaatttaaa ggtaaattag aaggaataaa aagcccacga gacattggaa tgaagattaa agattatatt gagaaaaatg gtgatttgtc aataaatttt aaatccatag aagttgcacc acaaggattt attacaataa agttatcaga tgaatttatt caaaataatt tatctaaatt acaagaaatg gatgaggtaa aagtaactcc aacaatgaaa ttccactgca aaagtggaag aaaaactcca aaagtaattg tagatttctc atcaccaaac attgccaaag aaatgcatgt tggacactta agatctacaa ttattggtga tacaacatgt agaattcttg aatatttagg atttgaagta ttaagagtta atcacgttgg agattggggt actcaatttg gcatgcttat tgaatattta aaggaagaat atcccacttt tgttgagaat ttaccagaaa tatcagattt acaagaattc tataagaact caaagaaaag gtttgatact gatccacaat ttaagttann nagccaacan nntgttgtta tgttacaagg gggnnatcaa gatgctttga ctgctnnnnn nnagatttgt gaggttagcc gagttgaatt nnnnnnaann nnnnntcgac ttnnnnnnnn nnntgaggnn nnnnnngaaa gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaan nnnnnnnnnn nnnnnngnnn nnnnnnnnnn nnnnnnnnnn nnnnnaatnn nnnnnnnnnn nnnnnngnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttagntnnn gnnnnnntnn nnnanactan nnnnnnnggc nnnnnnnnnc annnnntgaa actttnnnnn nnnnnnnnnn ntatgggatg gcatgatcnn nnnaatacan nnnnnttttt cattggatnn nnagttattc aaggagaaga tggaaaaaag tttaaaacaa gaagtggtga tgttgtcaaa ttaactgaac ttattgacga aggtgttgct agagcaaaga aagaattagt tgacagaaga aatcaaagaa gatctgatca aacaaataaa tcaaatacag atgttgaagt tgatcatcca aatattgatg attctgnnnt ggatttaatg gcaaatacta ttggtcatgc agctattaag tactttgatt tgaagcaaaa tagacttact aattacagat tttcatttga tcgaatgttg gatccaaaag gtaatactgc agtttattta ctatatgcat atgcaagaat ttgtgcaatc tttagaaaag ttgaattcat tcaatctgat aatagtatgg taattaaaca gaaagtttca aactttaagc ttaagatttc taataatgtt gaacgaaaat tgtctattca gttactcaga ttccctgaag tttttgatgg aattttgaat gatttcttcc ccaatagact tnnngattat tgttatgaat tatccgagtg ttttgcacaa ttttatactg aatgcagaat tattggatta gaagaacagc aacttgaaca aagtaggctt ttactttgtt tcttaacaaa acaaattctt ttaaaaggac ttgattta

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)