CrneC.20222.a

mRNA-capping enzyme subunit beta (CET1) (CNAG_06549)

CENTER ID: CrneC.20222.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.20222.a.B1.GE42743 full length 1 397
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_06549
UniProt: J9VT84

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTYIPIHDR PPHYANLPSP PSNSGQLRPL RYSSPDIDPD TPLPRMSSTH ESDPPEQINT THSGNHAPRQ RVGSQGAEQE GQSRKRARIS PSHLGGNGSY VPRQRTEKSH HPHHDDHSGH ATSDQHGNGQ VYRPRHGPAP LTGSIFNLSP RNPFTSVVGD FIMNAAMGHS NVEIELKLGT FMTPSMPGQQ PRRINMPTLS EMIIPHDYPN GPFVSTINHL HHRALNELLN RAVESQSTHP TGRLYFSRSK LADSFYDHSE HGRGKVRVSR DMDNGHVVQA VEKRRIADMN VYCPGMAYDF RISVNTETPC EVPTGNAKSV RYKDRACYRH QVCRVDLTSV FSSNPRNADV PPSRSFELEI EVLDVPALLA EGAAQSERFD EILQNVLDSA RMLVKNI
NT Sequence
atgaccacat acatccctat tcacgatcgt ccgcctcatt acgccaatct tccatccccg ccttccaaca gcggacagct cagaccactg cgttactcca gtccagatat cgatcccgac actccactcc caagaatgtc atcgactcat gaatcagatc ctccggagca aatcaatact acacattcag ggaatcatgc cccaagacag agagtaggga gccaaggagc cgagcaagaa ggacagtctc gaaaaagggc taggatttct ccgtcacact taggagggaa tggcagttac gttccgagac agcggacaga gaagagtcat cacccgcatc acgatgatca ttctggacat gcaacatccg atcaacatgg taatggacag gtatatcgac caaggcatgg tccggctccc ttgactggtt ccatcttcaa cctttcaccg agaaatccgt ttacgtctgt cgtcggtgac tttatcatga atgctgccat gggccatagc aatgttgaaa ttgagctcaa attggggacg ttcatgactc catcaatgcc cggtcagcaa ccacggcgga tcaacatgcc cacactcagt gagatgatca taccacacga ttatccgaat ggaccattcg tttcaactat caatcatttg caccatcgtg cgcttaatga actcctcaat cgcgccgtcg aatctcagag tacccaccct accggacgcc tttacttttc ccgctccaag ctcgccgact cgttctacga tcatagcgag catgggcgcg gtaaagtgcg ggtttcgagg gatatggata atggacacgt cgttcaggct gtagagaaga ggagaattgc ggatatgaat gtgtactgtc ctggtatggc gtacgatttt agaatcagtg tgaatactga aacgccttgt gaagtaccaa cgggaaacgc aaaatctgtg cgatacaagg atagagcgtg ttacagacat caagtctgtc gggttgatct cactagcgta ttctcttcta atccaagaaa cgccgatgtt ccaccttcgc gatcattcga gctcgaaatt gaagttcttg acgtcccagc attacttgca gaaggcgctg ctcaaagcga acggtttgac gagatcttac agaatgtcct tgatagcgct cggatgttgg tgaagaatat ataa
Details for CrneC.20222.a.B1.GE42743
HARVESTED ON: 11/5/2018
SEQUENCED ON: 11/7/2018
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 86
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMT TYIPIHDRPP HYANLPSPPS NSGQLRPLRY SSPDIDPDTP LPRMSSTHES DPPEQINTTH SGNHAPRQRV GSQGAEQEGQ SRKRARISPS HLGGNGSYVP RQRTEKSHHP HHDDHSGHAT SDQHGNGQVY RPRHGPAPLT GSIFNLSPRN PFTSVVGDFI MNAAMGHSNV EIELKLGTFM TPSMPGQQPR RINMPTLSEM IIPHDYPNGP FVSTINHLHH RALNELLNRA VESQSTHPTG RLYFSRSKLA DSFYDHSEHG RGKVRVSRDM DNGHVVQAVE KRRIADMNVY CPGMAYDFRI SVNTETPCEV PTGNANLCDT RIERVTDIKS VGLISLAYSL LIQETPMFHL RDHSSSKLKF LTSQHYLQKA LLKANGLTRS YRMSL
Validated NT Sequence
tatcaaggac attctgtaag atctcgtcaa accgttcgct ttgagcagcg ccttctgcaa gtaatgctgg gacgtcaaga acttcaattt cgagctcgaa tgatcgcgaa ggtggaacat cggcgtttct tggattagaa gagaatacgc tagtgagatc aacccgacag acttgatgtc tgtaacacgc tctatccttg tatcgcacag atttgcgttt cccgttggta cttcacaagg cgtttcagta ttcacactga ttctaaaatc gtacgccata ccaggacagt acacattcat atccgcaatt ctcctcttct ctacagcctg aacgacgtgt ccattatcca tatccctcga aacccgcact ttaccgcgcc catgctcgct atgatcgtag aacgagtcgg cgagcttgga gcgggaaaag taaaggcgtc cggtagggtg ggtactctga gattcgacgg cgcgattgag gagttcatta agcgcacgat ggtgcaaatg attgatagtt gaaacgaatg gtccattcgg ataatcgtgt ggtatgatca tctcactgag tgtgggcatg ttgatccgcc gtggttgctg accgggcatt gatggagtca tgaacgtccc caatttgagc tcaatttcaa cattgctatg gcccatggca gcattcatga taaagtcacc gacgacagac gtaaacggat ttctcggtga aaggttgaag atggaaccag tcaagggagc cggaccatgc cttggtcgat atacctgtcc attaccatgt tgatcggatg ttgcatgtcc agaatgatca tcgtgatgcg ggtgatgact cttctctgtc cgctgtctcg gaacgtaact gccattccct cctaagtgtg acggagaaat cctagccctt tttcgagact gtccttcttg ctcggctcct tggctcccta ctctctgtct tggggcatga ttccctgaat gtgtagtatt gatttgctcc ggaggatctg attcatgagt cgatgacatt cttgggagtg gagtgtcggg atcgatatct ggactggagt aacgcagtgg tctgagctgt ccgctgttgg aaggcgggga tggaagattg gcgtaatgag gcggacgatc gtgaataggg atgtatgtgg tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMT TYIPIHDRPP HYANLPSPPS NSGQLRPLRY SSPDIDPDTP LPRMSSTHES DPPEQINTTH SGNHAPRQRV GSQGAEQEGQ SRKRARISPS HLGGNGSYVP RQRTEKSHHP HHDDHSGHAT SDQHGNGQVY RPRHGPAPLT GSIFNLSPRN PFTSVVGDFI MNAAMGHSNV EIELKLGTFM TPSMPGQQPR RINMPTLSEM IIPHDYPNGP FVSTINHLHH RALNELLNRA VESQSTHPTG RLYFSRSKLA DSFYDHSEHG RGKVRVSRDM DNGHVVQAVE KRRIADMNVY CPGMAYDFRI SVNTETPCEV PTGNAKSVRY KDRACYRHQV CRVDLTSVFS SNPRNADVPP SRSFELEIEV LDVPALLAEG AAQSERFDEI LQNVLDSARM LVKNI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacca catacatccc tattcacgat cgtccgcctc attacgccaa tcttccatcc ccgccttcca acagcggaca gctcagacca ctgcgttact ccagtccaga tatcgatccc gacactccac tcccaagaat gtcatcgact catgaatcag atcctccgga gcaaatcaat actacacatt cagggaatca tgccccaaga cagagagtag ggagccaagg agccgagcaa gaaggacagt ctcgaaaaag ggctaggatt tctccgtcac acttaggagg gaatggcagt tacgttccga gacagcggac agagaagagt catcacccgc atcacgatga tcattctgga catgcaacat ccgatcaaca tggtaatgga caggtatatc gaccaaggca tggtccggct cccttgactg gttccatctt caacctttca ccgagaaatc cgtttacgtc tgtcgtcggt gactttatca tgaatgctgc catgggccat agcaatgttg aaattgagct caaattgggg acgttcatga ctccatcaat gcccggtcag caaccacggc ggatcaacat gcccacactc agtgagatga tcataccaca cgattatccg aatggaccat tcgtttcaac tatcaatcat ttgcaccatc gtgcgcttaa tgaactcctc aatcgcgccg tcgaatctca gagtacccac cctaccggac gcctttactt ttcccgctcc aagctcgccg actcgttcta cgatcatagc gagcatgggc gcggtaaagt gcgggtttcg agggatatgg ataatggaca cgtcgttcag gctgtagaga agaggagaat tgcggatatg aatgtgtact gtcctggtat ggcgtacgat tttagaatca gtgtgaatac tgaaacgcct tgtgaagtac caacgggaaa cgcaaaatct gtgcgataca aggatagagc gtgttacaga catcaagtct gtcgggttga tctcactagc gtattctctt ctaatccaag aaacgccgat gttccacctt cgcgatcatt cgagctcgaa attgaagttc ttgacgtccc agcattactt gcagaaggcg ctgctcaaag cgaacggttt gacgagatct tacagaatgt ccttgatagc gctcggatgt tggtgaagaa tatatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat