CrneC.20217.a

tRNA-splicing endonuclease subunit Sen54 (SEN54) (CNAG_02564)

CENTER ID: CrneC.20217.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.20217.a.B1.GE42740 full length 1 552
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_02564
UniProt: J9VNE5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEHITDIQAG PSKLKASQTP QKKPRKSTPS TPVINDDGDD SGPANAKAIS GPATGGQEDE EEEGEERMDF KLIQSFADKI QLLPTADNVD GLTSRPQIII PRRGEKDFEP LQETANLQEM MLEKGRQALF NALTGVRGGH NNSISHALLT PKKPFPQVLI VHGHLLDTMG ITVRTPPPAG SKGKGKTSLE LLPEEALYLL ERGSLQIWIG KDPETDEEVE AGVGEWCDEE YGVKGAIEMS VMEGFGAFIG REGLTWERYQ AYSYLKRLGY NVQRSRQFIP EYFLAAPSVQ LDEQDPRLPL FNTWWLNIPK WIVGFFKVLA RGVQYVAGKM ARVGLGLRLT RDPFKGTLLN GWKGSSYNSL FSYLRVVPAG HNQPLPPRPS APPSDDIYAP LIHNPYIPFW HIWKPMTLWS KRNWDKGSEE GLKTQRPDYF AAVIQARITP LPTIHQLEEI YAMLPDEPKG PIKRYGPQYQ RPPRPQVSKP QTQTPAQIPS RLQSLLEHMG WKQQAKTKPQ APSVNVGALR NGDRGFIVGV NDSGNNAWIR FGRTDFSQTP AI
NT Sequence
atggaacaca tcacagatat tcaggcaggt ccgagcaagc tgaaggcttc tcagactccc cagaagaaac caagaaaatc caccccttca actcctgtaa tcaacgacga tggtgacgac tctggacccg ccaatgcaaa agctatttcg ggccctgcaa caggtggaca ggaggacgaa gaggaggagg gtgaagagag gatggatttt aaacttattc agagctttgc cgataaaatt caactcctac ctacggccga caacgtggat ggtttgacgt ctcgaccgca aatcatcatt cctagacgtg gagaaaagga ctttgagcca ctacaggaga ctgccaatct acaagagatg atgttggaaa agggccgaca agcattgttc aacgctctca ccggtgtgcg tggaggtcac aataactcta tatcccatgc cctcttgaca cccaagaagc cgtttcctca agtgttaatt gttcatggac atctattgga cactatgggt atcactgttc gaactcctcc tccggctggt tccaaaggca aaggcaaaac gagccttgaa cttttacctg aagaggctct ctacctgcta gaacgtggat cgctgcaaat atggatagga aaggaccccg agacggatga agaagttgaa gcaggcgttg gggaatggtg cgatgaagag tacggggtca agggcgctat tgaaatgagc gttatggaag gtttcggggc gttcatcggc cgagaaggat tgacttggga gcgttaccag gcatattcgt atcttaaacg tctcggctac aatgtccaaa ggtctcgtca gttcattcca gaatacttct tagcggcacc ctcagtgcag cttgatgaac aagatccccg gttgccgctc ttcaacactt ggtggttgaa tattcctaag tggattgtag gattctttaa agtgttggct agaggtgtgc aatatgtggc tggaaagatg gcgcgcgttg gtttggggtt acgcttgaca agggatccgt ttaagggtac tctgttgaat ggctggaaag gttcaagtta caactcatta ttctcctatc tgcgggttgt tcctgctggt cacaaccagc ccctcccgcc tcgcccatcc gctcctcctt ctgacgacat ctacgctcct cttatccaca acccttacat tccattctgg catatctgga agcctatgac cctttggtca aagaggaatt gggataaggg cagcgaggaa gggctcaaaa cccagagacc tgattatttt gcagctgtca tccaagcaag gattacgcct ctgcctacta ttcaccaact tgaagaaatt tacgctatgc tgcccgatga acccaaaggc cctatcaagc gatatggtcc ccaataccag cggcctcctc gtccccaggt gtcaaaaccc caaacacaga cccccgctca gattccttct cgtttacaat ctctcttaga gcatatgggc tggaagcaac aggcgaaaac aaagccccag gcccctagtg tcaacgttgg ggcgctgcgt aatggcgata gaggattcat tgtgggtgtc aatgattcag gtaataatgc ttggattcgt ttcggaagaa cagacttttc gcagacccca gcaatctag
Details for CrneC.20217.a.B1.GE42740
HARVESTED ON: data unavailable
SEQUENCED ON: 11/7/2018
EXPECTED MW: 63kDa
OBSERVED MW: 15kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 35
Validated AA Sequence
MAHHHHHHME HITDIQAGPS KLKASQTPQK KPRKSTPSTP VINDDGDDSG PANAKAISGP ATGGQEDEEE EGEERMDFKL IQSFADKIQL LPTADNVDGL TSRPQIIIPR RGEKDFEPLQ ETANLQEMML EKGRQALFNA LTGVRGGHNN SISHALLTPK KPFPQVLIVH GHLLDTMGIT VRTPPPAGSX XXXXXXXELL P
Validated NT Sequence
agtctgttct tccgaaacga atccaagcat tattacctga atcattgaca cccacaatga atcctctatc gccattacgc agcgccccaa cgttgacact aggggcctgg ggctttgttt tcgcctgttg cttccagccc atatgctcta agagagattg taaacgagaa ggaatctgag cgggggtctg tgtttggggt tttgacacct ggggacgagg aggccgctgg tattggggac catatcgctt gatagggcct ttgggttcat cgggcagcat agcgtaaatt tcttcaagtt gatgaatagt aggcagaggc gtaatccttg cttggatgac agctgcaaaa taatcaggtc tctgggtttt gagcccttcc tcgctgccct tatcccaatt cctctttgac caaagggtca taggcttcca gatatgccag aatggaatgt aagggttgtg gataagagga gcgtagatgt cgtcagaagg aggagcggat gggcgaggtg ggaggggctg gttgtgacca gcaggaacaa cccgcagata ggagaataat gagttgtaac ttgaaccttt ccagccattc aacagagtac nnntaaacgg atcccttgtc aagcgtaacc ccaaaccaac gcgcgccatc tttnnagcca catattgcac acctctannn nacactnnna nnnnnnctac aatccacnta nnnnnnttnn nnnnnnnnnn nnngannnnn nnnnnnngng nnnnnnnnnn nnnnnnnnnn ncnnnnnnnn cnnnnannnn nnnnnnnnga atnnnnnnnn nnnnnnnnnn nnnnntgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncccc nnnnnnnnnn tnnnnnnctt catcnnnnnn nnnnnnnnnn nnnatncnnn nnnncagcga nnnnnnnnnn nncnggtaga gnnnnncttc agggtaaaag ttcannnctc gnnnnnnnnn nnnnnttgga accagccgga ggaggagttc gaacagtgat acccatagtg tccaatagat gtccatgaac aattaacact tgaggaaacg gcttcttggg tgtcaagagg gcatgggata tagagttatt gtgacctcca cgcacaccgg tgagagcgtt gaacaatgct tgtcggccct tttccaacat catctcttgt agattggcag tctcctgtag tggctcaaag tccttttctc cacgtctagg aatgatgatt tgcggtcgag acgtcaaacc atccacgttg tcggccgtag gtaggagttg aattttatcg gcaaagctct gaataagttt aaaatccatc ctctcttcac cctcctcctc ttcgtcctcc tgtccacctg ttgcagggcc cgaaatagct tttgcattgg cgggtccaga gtcgtcacca tcgtcgttga ttacaggagt tgaaggggtg gattttcttg gtttcttctg gggagtctga gaagccttca gcttgctcgg acctgcctga atatctgtga tgtgttccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHME HITDIQAGPS KLKASQTPQK KPRKSTPSTP VINDDGDDSG PANAKAISGP ATGGQEDEEE EGEERMDFKL IQSFADKIQL LPTADNVDGL TSRPQIIIPR RGEKDFEPLQ ETANLQEMML EKGRQALFNA LTGVRGGHNN SISHALLTPK KPFPQVLIVH GHLLDTMGIT VRTPPPAGSK GKGKTSLELL PEEALYLLER GSLQIWIGKD PETDEEVEAG VGEWCDEEYG VKGAIEMSVM EGFGAFIGRE GLTWERYQAY SYLKRLGYNV QRSRQFIPEY FLAAPSVQLD EQDPRLPLFN TWWLNIPKWI VGFFKVLARG VQYVAGKMAR VGLGLRLTRD PFKGTLLNGW KGSSYNSLFS YLRVVPAGHN QPLPPRPSAP PSDDIYAPLI HNPYIPFWHI WKPMTLWSKR NWDKGSEEGL KTQRPDYFAA VIQARITPLP TIHQLEEIYA MLPDEPKGPI KRYGPQYQRP PRPQVSKPQT QTPAQIPSRL QSLLEHMGWK QQAKTKPQAP SVNVGALRNG DRGFIVGVND SGNNAWIRFG RTDFSQTPAI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaac acatcacaga tattcaggca ggtccgagca agctgaaggc ttctcagact ccccagaaga aaccaagaaa atccacccct tcaactcctg taatcaacga cgatggtgac gactctggac ccgccaatgc aaaagctatt tcgggccctg caacaggtgg acaggaggac gaagaggagg agggtgaaga gaggatggat tttaaactta ttcagagctt tgccgataaa attcaactcc tacctacggc cgacaacgtg gatggtttga cgtctcgacc gcaaatcatc attcctagac gtggagaaaa ggactttgag ccactacagg agactgccaa tctacaagag atgatgttgg aaaagggccg acaagcattg ttcaacgctc tcaccggtgt gcgtggaggt cacaataact ctatatccca tgccctcttg acacccaaga agccgtttcc tcaagtgtta attgttcatg gacatctatt ggacactatg ggtatcactg ttcgaactcc tcctccggct ggttccaaag gcaaaggcaa aacgagcctt gaacttttac ctgaagaggc tctctacctg ctagaacgtg gatcgctgca aatatggata ggaaaggacc ccgagacgga tgaagaagtt gaagcaggcg ttggggaatg gtgcgatgaa gagtacgggg tcaagggcgc tattgaaatg agcgttatgg aaggtttcgg ggcgttcatc ggccgagaag gattgacttg ggagcgttac caggcatatt cgtatcttaa acgtctcggc tacaatgtcc aaaggtctcg tcagttcatt ccagaatact tcttagcggc accctcagtg cagcttgatg aacaagatcc ccggttgccg ctcttcaaca cttggtggtt gaatattcct aagtggattg taggattctt taaagtgttg gctagaggtg tgcaatatgt ggctggaaag atggcgcgcg ttggtttggg gttacgcttg acaagggatc cgtttaaggg tactctgttg aatggctgga aaggttcaag ttacaactca ttattctcct atctgcgggt tgttcctgct ggtcacaacc agcccctccc gcctcgccca tccgctcctc cttctgacga catctacgct cctcttatcc acaaccctta cattccattc tggcatatct ggaagcctat gaccctttgg tcaaagagga attgggataa gggcagcgag gaagggctca aaacccagag acctgattat tttgcagctg tcatccaagc aaggattacg cctctgccta ctattcacca acttgaagaa atttacgcta tgctgcccga tgaacccaaa ggccctatca agcgatatgg tccccaatac cagcggcctc ctcgtcccca ggtgtcaaaa ccccaaacac agacccccgc tcagattcct tctcgtttac aatctctctt agagcatatg ggctggaagc aacaggcgaa aacaaagccc caggccccta gtgtcaacgt tggggcgctg cgtaatggcg atagaggatt cattgtgggt gtcaatgatt caggtaataa tgcttggatt cgtttcggaa gaacagactt ttcgcagacc ccagcaatct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat