CrneC.19525.a

3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) (EC 1.1.1.34)

CENTER ID: CrneC.19525.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19525.a.B2.GE41077 soluble domain 722 1224
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_06534
UniProt: J9VT67
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRQTLVSLS SLAASAPIEV ITTTFIFATL AYFQLLHAIK GSEFFNVPSV SPPSRPAHFV RLSHQPTSED ASYILPSSHG VAGSSWVGDV ESKREWTPIS PSDFRKILEQ NALGGYTFPI EAGGNTSGEK ASVAIVKQVV VTREGAEEPI DEFEQWLLNV LSVEVDGEKY TYRDLCFNTS DHPSFVSHPL YPSQSTLTMF LQPPSPSDPT LTYLNRLNRL PAFTLSNSTT AFQLLPPTGA SWGFLPSIDG VGLFSKLGEG MISGQGESYP MQNVRWFAYA ARALVGRFWT LAKNADSADI FVVLLGYIVM HGVFVNLFIG MRKLGSSFWI PVATLCSSTF AFLVALLSAY LLDLPVDPIC LSEAIPFLVI TVGFDKPYHL AKAVLQNPDI DPVPSSPEPS TAGYSGELSK DPNSGSTLGL DLGTLHKELM PLERLQRLAE GQVKWVAPVA AKKIVVDAVR TSGVRIVRDY VLEIIALCVG AASGIGGLRE FCYLAALIMA VDCVFLFTFY VAILSVMVEV HRIKLIRGNR RVKSVKRVSS SASLNSHGNG NGNGSTTNTS SSTSSPSTKR SFWSRSPSSE REDQPQNPLV RLKLLLIISF LTLHVLNLCT TLTEQTALKR HSTHSGAEIT SRAMLNPRGI SLSPVLQALY ENQPPETDLA VQVMPPTQIL MTSETFIPSR LSSFDQFMSE WTLLVGDPVL SKWIVVFLAI SVLLNGYLLK GIALNSMGGK GPVAAAAQAL AGIFDAERGQ RALRESTPRG RKTELPAHMV GTNASTSTRD GDSTPRVEEV GKVTATHTVN VPIIKEPRIP SPSFPSASDD SALTFGRRSF EECVEIYAGG VGANNLSDEE IILLVQKGKI APYGLEKALK NLERAVRIRR AVISRSSLTK SLETSLLPMA DYDYKQVIGA CCENVIGYLP LPVGIAGPLN IDGQLLHIPM ATTEGTLVAS TSRGCKALNA GGGVTTVLTH DAMTRGPAID FPNIVQAAQA RLWIDSPDGY ATLKAAFEST SRFAKLQTLE CALAGRTLYV RFATQTGDAM GMNMISKGVE KALEVLRNHF PDMHVLALSG NYCTDKKPAA INWIEGRGKS VVAEAVVPGH VVKTVLKTTV KDLCNLNIKK NLIGSAMAGS IGGFNAHAAN ILTAMYLACG QDPAQNVESS NCMTLMEPIN DGEDLLISCS MPSVEVGTVG GGTILSPQRA MLDMLGVAGA HATSPGANAQ RLARIIVAAV MAGELSLMSA LAAGHLIQAH MKHNRSTPAT PGAVTPFGGI TPLREAVLIN GPGAK
NT Sequence
atgcttcgcc aaacactcgt ctctctctcg tccttggccg cctccgcgcc catcgaggtc atcaccacca ccttcatctt cgccaccctc gcatacttcc agctcctcca cgcgatcaag ggctccgagt ttttcaacgt cccttccgtc tccccgcctt cccgccccgc ccatttcgtc cgtctctccc accagcccac ctctgaagac gcgtcctaca tccttcccag ctcgcacggt gtcgccggga gcagctgggt gggcgatgtc gagtccaaac gcgaatggac acccatctct ccatctgatt tccgaaaaat cctcgaacag aatgctcttg gtggatacac ctttcccatc gaggcaggcg gtaacacttc tggtgaaaag gccagcgtcg cgatcgtcaa gcaggtcgtc gtcacgcgtg aaggcgcaga ggagccaatt gatgagtttg aacaatggtt attaaatgtg ctgagtgtag aagtcgacgg ggaaaagtac acttatcgcg acttgtgctt caacacctct gaccaccctt cattcgtctc tcaccctctc tacccatccc aatccaccct caccatgttc ctccaacctc cctcaccttc agaccctact ctcacctacc tcaacaggct taaccgcctt ccggcgttta ccctctcaaa ctcgaccacc gctttccaac tcttaccccc aacgggcgcg agctgggggt tcttacccag tatcgatggt gtcggtttgt tctccaagtt gggcgaggga atgatctctg gtcaagggga gagttacccc atgcagaacg ttaggtggtt tgcctatgct gctagagcgt tggtcggcag attttggact ttggcaaaga atgccgactc tgctgatatt tttgtggtgc ttctcggcta catcgtcatg cacggtgtgt ttgtcaactt gttcatcggt atgcgcaaac tcggaagctc tttctggatc cctgttgcaa ccctctgctc ttcaacattc gcattcctcg tcgccctcct ctctgcttat ctcctcgacc tccccgtcga ccccatctgt ctttctgaag cgatcccctt cctcgtcatc acagtcggct ttgacaaacc ctaccacctc gccaaagccg tcttgcaaaa ccccgatatc gatcccgtcc cttcatctcc cgaaccttct actgctggtt acagcggcga attgtcaaaa gatcccaact ctggctccac tttaggactt gatcttggga ctttgcacaa ggaacttatg cctcttgagc gtcttcagcg attggcggaa ggacaggtca agtgggtcgc ccctgtggct gccaagaaga ttgtagtcga cgctgtgagg acatcgggtg tgaggattgt tagggattac gttttggaga tcattgcgct ttgcgtcggt gcagctagtg ggatcggtgg tctgagagaa ttttgctatc ttgccgcatt aatcatggcc gtcgattgtg tcttcctctt cacgttttac gttgccatcc tcagcgtcat ggtcgaagtt caccgtatca agcttattcg aggtaaccgc cgagtcaaat ctgtcaaacg cgtctctagc tctgcctctc ttaacagcca cggcaacggc aacggcaacg gcagcactac caacaccagc agcagcactt ccagtccctc cactaagcga agcttttggt ctcgctcccc ctccagcgag agagaagacc aacctcaaaa ccctcttgtt cgactaaaac tcctcttgat catctctttc ctaactctcc acgttctcaa cctctgtacg accctcaccg agcaaaccgc cttaaaacgt cactccaccc attctggcgc tgaaatcacc tctcgcgcga tgctcaaccc ccgcggtatc tcactctccc ccgtcctcca ggcgctctac gagaatcaac cccccgagac tgacttggcc gtccaagtca tgccccctac tcaaattctc atgacaagcg aaacctttat ccccagccgc ttgtcctctt tcgaccagtt tatgagcgaa tggacgttac tcgtgggtga tcccgtgctg agcaagtgga ttgtcgtctt cctcgcgatc agtgtattat tgaatggcta tctcttaaag ggaattgcgt tgaattcaat gggcggcaaa gggccagtgg cggcggcggc tcaagcgtta gctgggatct ttgatgctga gcgaggacag agagctcttc gagagtcgac gccacgaggg aggaagaccg agttgcctgc gcacatggtt ggtacaaatg caagtactag tactcgggat ggggattcga cacctcgagt tgaagaagtt ggcaaggtta ctgctaccca cactgtcaac gtacctatca tcaaggaacc tcgtatccct tccccttcat tcccctctgc ttccgatgac tctgctctta cctttggccg acgatcgttt gaggaatgtg tcgagattta cgcaggcggc gttggcgcca acaatctttc cgacgaggaa attatcttgc tcgtccaaaa gggcaagatt gcgccgtacg gccttgaaaa agctttaaag aacctcgaac gagctgtccg aattcgacga gccgtcatct cccgatccag tctcaccaaa tctcttgaga cgagtttgct ccctatggca gactatgatt acaagcaagt catcggtgca tgctgcgaga atgtgattgg ttaccttcct cttccggtcg gcatcgccgg tcctctcaac attgatggtc aactgttgca tatccccatg gctaccaccg aaggtactct tgtcgcttct acatctcgag gatgtaaagc cctcaacgcc ggtggcggcg tcacaaccgt cctcactcac gacgccatga cccgcggccc agctatcgat ttccccaaca ttgtccaagc tgcccaagca cgtctatgga tcgattcgcc cgacgggtac gccaccctca aagctgcctt tgagtccacc tctcgattcg ccaaactcca gactctggaa tgcgctttgg caggtcggac gttgtatgtg aggtttgcga cccagaccgg tgacgcgatg ggtatgaaca tgatctccaa gggggtcgaa aaggctctcg aagtcctgcg caatcatttc cccgatatgc acgtcctcgc tctttccgga aactattgta ccgacaagaa accggcggcg atcaattgga ttgaagggag aggtaagagc gtggtggcgg aagcggtggt gcccggccat gtggtgaaga cggtgttgaa gacgacagtg aaggatttgt gtaatttgaa catcaagaag aatttgattg gaagtgcgat ggcgggaagt ataggaggat ttaacgctca cgcggcgaat atcttgactg ccatgtacct cgcgtgcggt caggatccgg ctcagaacgt tgaaagttcc aactgtatga ctcttatgga accgatcaac gacggcgaag accttctcat ctcatgttcc atgccttccg ttgaagtcgg caccgtcggc ggcggtacca tactttcccc ccagcgcgcc atgctcgaca tgctcggcgt cgctggtgcc catgccactt cgccaggtgc aaacgcccaa cgtctcgctc gaatcattgt cgcagccgtc atggctggtg aactgagttt gatgagtgcg ctcgctgcgg gtcatttgat tcaggcgcat atgaagcata ataggagtac gcctgcgacg ccgggggcag tgacgccgtt tggagggata acgccgttga gggaggctgt gttgattaat ggcccgggag cgaagtga
Details for CrneC.19525.a.B2.GE41077
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHHM IALNSMGGKG PVAAAAQALA GIFDAERGQR ALRESTPRGR KTELPAHMVG TNASTSTRDG DSTPRVEEVG KVTATHTVNV PIIKEPRIPS PSFPSASDDS ALTFGRRSFE ECVEIYAGGV GANNLSDEEI ILLVQKGKIA PYGLEKALKN LERAVRIRRA VISRSSLTKS LETSLLPMAD YDYKQVIGAC CENVIGYLPL PVGIAGPLNI DGQLLHIPMA TTEGTLVAST SRGCKALNAG GGVTTVLTHD AMTRGPAIDF PNIVQAAQAR LWIDSPDGYA TLKAAFESTS RFAKLQTLEC ALAGRTLYVR FATQTGDAMG MNMISKGVEK ALEVLRNHFP DMHVLALSGN YCTDKKPAAI NWIEGRGKSV VAEAVVPGHV VKTVLKTTVK DLCNLNIKKN LIGSAMAGSI GGFNAHAANI LTAMYLACGQ DPAQNVESSN CMTLMEPIND GEDLLISCSM PSVEVGTVGG GTILSPQRAM LDMLGVAGAH ATSPG
Validated NT Sequence
tgcacctggc gaagtggcat gggcaccagc gacgccgagc atgtcgagca tggcgcgctg gggggaaagt atggtaccgc cgccgacggt gccgacttca acggaaggca tggaacatga gatgagaagg tcttcgccgt cgttgatcgg ttccataaga gtcatacagt tggaactttc aacgttctga gccggatcct gaccgcacgc gaggtacatg gcagtcaaga tattcgccgc gtgagcgtta aatcctccta tacttcccgc catcgcactt ccaatcaaat tcttcttgat gttcaaatta cacaaatcct tcactgtcgt cttcaacacc gtcttcacca catggccggg caccaccgct tccgccacca cgctcttacc tctcccttca atccaattga tcgccgccgg tttcttgtcg gtacaatagt ttccggaaag agcgaggacg tgcatatcgg ggaaatgatt gcgcaggact tcgagagcct tttcgacccc cttggagatc atgttcatac ccatcgcgtc accggtctgg gtcgcaaacc tcacatacaa cgtccgacct gccaaagcgc attccagagt ctggagtttg gcgaatcgag aggtggactc aaaggcagct ttgagggtgg cgtacccgtc gggcgaatcg atccatagac gtgcttgggc agcttggaca atgttgggga aatcgatagc tgggccgcgg gtcatggcgt cgtgagtgag gacggttgtg acgccgccac cggcgttgag agctttacat cctcgagatg tagaagcgac aagagtacct tcggtggtag ccatggggat atgcaacagt tgaccatcaa tgttgagagg accggcgatg ccgaccggaa gaggaaggta accaatcaca ttctcgcagc atgcaccgat gacttgcttg taatcatagt ctgccatagg gagcaaactc gtctcaagag atttggtgag actggatcgg gagatgacgg ctcgtcgaat tcggacagct cgttcgaggt tctttaaagc tttttcaagg ccgtacggcg caatcttgcc cttttggacg agcaagataa tttcctcgtc ggaaagattg ttggcgccaa cgccgcctgc gtaaatctcg acacattcct caaacgatcg tcggccaaag gtaagagcag agtcatcgga agcagagggg aatgaagggg aagggatacg aggttccttg atgataggta cgttgacagt gtgggtagca gtaaccttgc caacttcttc aactcgaggt gtcgaatccc catcccgagt actagtactt gcatttgtac caaccatgtg cgcaggcaac tcggtcttcc tccctcgtgg cgtcgactct cgaagagctc tctgtcctcg ctcagcatca aagatcccag ctaacgcttg agccgccgcc gccactggcc ctttgccgcc cattgaattc aacgcaatca tatggtggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHIA LNSMGGKGPV AAAAQALAGI FDAERGQRAL RESTPRGRKT ELPAHMVGTN ASTSTRDGDS TPRVEEVGKV TATHTVNVPI IKEPRIPSPS FPSASDDSAL TFGRRSFEEC VEIYAGGVGA NNLSDEEIIL LVQKGKIAPY GLEKALKNLE RAVRIRRAVI SRSSLTKSLE TSLLPMADYD YKQVIGACCE NVIGYLPLPV GIAGPLNIDG QLLHIPMATT EGTLVASTSR GCKALNAGGG VTTVLTHDAM TRGPAIDFPN IVQAAQARLW IDSPDGYATL KAAFESTSRF AKLQTLECAL AGRTLYVRFA TQTGDAMGMN MISKGVEKAL EVLRNHFPDM HVLALSGNYC TDKKPAAINW IEGRGKSVVA EAVVPGHVVK TVLKTTVKDL CNLNIKKNLI GSAMAGSIGG FNAHAANILT AMYLACGQDP AQNVESSNCM TLMEPINDGE DLLISCSMPS VEVGTVGGGT ILSPQRAMLD MLGVAGAHAT SPGANAQRLA R
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattgcgt tgaattcaat gggcggcaaa gggccagtgg cggcggcggc tcaagcgtta gctgggatct ttgatgctga gcgaggacag agagctcttc gagagtcgac gccacgaggg aggaagaccg agttgcctgc gcacatggtt ggtacaaatg caagtactag tactcgggat ggggattcga cacctcgagt tgaagaagtt ggcaaggtta ctgctaccca cactgtcaac gtacctatca tcaaggaacc tcgtatccct tccccttcat tcccctctgc ttccgatgac tctgctctta cctttggccg acgatcgttt gaggaatgtg tcgagattta cgcaggcggc gttggcgcca acaatctttc cgacgaggaa attatcttgc tcgtccaaaa gggcaagatt gcgccgtacg gccttgaaaa agctttaaag aacctcgaac gagctgtccg aattcgacga gccgtcatct cccgatccag tctcaccaaa tctcttgaga cgagtttgct ccctatggca gactatgatt acaagcaagt catcggtgca tgctgcgaga atgtgattgg ttaccttcct cttccggtcg gcatcgccgg tcctctcaac attgatggtc aactgttgca tatccccatg gctaccaccg aaggtactct tgtcgcttct acatctcgag gatgtaaagc cctcaacgcc ggtggcggcg tcacaaccgt cctcactcac gacgccatga cccgcggccc agctatcgat ttccccaaca ttgtccaagc tgcccaagca cgtctatgga tcgattcgcc cgacgggtac gccaccctca aagctgcctt tgagtccacc tctcgattcg ccaaactcca gactctggaa tgcgctttgg caggtcggac gttgtatgtg aggtttgcga cccagaccgg tgacgcgatg ggtatgaaca tgatctccaa gggggtcgaa aaggctctcg aagtcctgcg caatcatttc cccgatatgc acgtcctcgc tctttccgga aactattgta ccgacaagaa accggcggcg atcaattgga ttgaagggag aggtaagagc gtggtggcgg aagcggtggt gcccggccat gtggtgaaga cggtgttgaa gacgacagtg aaggatttgt gtaatttgaa catcaagaag aatttgattg gaagtgcgat ggcgggaagt ataggaggat ttaacgctca cgcggcgaat atcttgactg ccatgtacct cgcgtgcggt caggatccgg ctcagaacgt tgaaagttcc aactgtatga ctcttatgga accgatcaac gacggcgaag accttctcat ctcatgttcc atgccttccg ttgaagtcgg caccgtcggc ggcggtacca tactttcccc ccagcgcgcc atgctcgaca tgctcggcgt cgctggtgcc catgccactt cgccaggtgc aaacgcccaa cgtctcgctc gatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat