CrneC.19443.a

TTK protein kinase, variant

CENTER ID: CrneC.19443.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19443.a.B2.GE41422 ordered domain 481 784
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_06697
UniProt: T2BP42

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSPENGRVL DNLWTTEDES FEVQMPDFHF DWGLEKGKTA EQGIKAPTAS LRSLSIKEIT PPLPSGSSST STPSHSTLSA RSSISSAQIL PKRGASNASS SPHLPTPPGS VANPGHRSVS VQNIANSGGS SGGESSGERG KAYGGRKFQR VTSAPISPED ERETERVEFQ VSGNGCSKSE SATNPHTTTI RPPHTQLPSA PLDDTSTVPN HSNFITPALP ERTLPIARST VRRLGGLSKF GGPARRVILP QGSEDVQVQE SVESSNVNEL QGRHNSAPIR PPDVYCSYDQ ASTSPTATAG CDNRLMNISH PDDRTVKSAR KPVNQSVHEI NESIHEPLAL ATQPQGEHGV SRISAALSSE GEDRLFRPFG KLEEKLRPVA GPSKASGLRY MDSLPETGPE ARDMQGNSPR SSDVGKSASP PYRNLRAARP PPVTESRRNA PMVSTATAVS APASHPMMSS EILQPPPTQS VPQGKTLFHV NGVPYERLQR LGKGGSSTVY SVLYSGPKKR IIYALKVVQL DRADSETYQS YTNEIELLKR LRGHDRVIQL IDHQITFNQH NRPHRLLMVM ECGEIDFAAL LDEQRGKAIN MNFVGLYWEQ MLEAVQAVHR ENVVHTDLKP ANFVLVKGRL KIIDFGIAKA IANDTVNIQR DQQIGTVNYM SPEAIQRMNN QKVLKLSYPS DVWSLGCILY QMIYGSPPFQ HVSGGPLAKM GVIADPNHVV TYPEVAVPKA AVGFSLDGHP TDPASLSVPV SPSAIDSMKR CLAYRKEHRL TIPELLQHEF LKPRIRAPAV PPGSTPITQY QMERLVNFIL SENGLPELSD GNHTAEDLFS QLEAQNALSR
NT Sequence
atgagctcac ccgagaacgg tcgcgtgctc gacaatctgt ggacaaccga ggacgagtcg ttcgaagtgc agatgccgga cttccatttt gattggggac tggagaaagg aaaaaccgca gagcaaggaa ttaaggcccc cactgcctcc ttgcgctctc tatctatcaa agaaatcact ccacccctcc ctagtgggtc ttcttctacc tccactcctt ctcattccac cttatcggca agatcgtcaa tatcttctgc ccagatatta cccaaaaggg gtgcctctaa tgcgtcctca tcacctcacc tacccactcc tcccggaagc gtcgccaacc ctggccatcg atcagtatct gttcaaaaca tcgccaattc tggtggaagc tccggtgggg agtctagcgg tgaaaggggc aaggcttatg gtggccgcaa gttccagcgc gtaacgtctg ccccaatatc gcccgaagat gaaagagaaa cggagagagt agaatttcaa gtttctggaa atggttgctc aaaatctgaa agtgcaacca atccacatac taccactata cgccctccgc atacacagct accgtctgca ccacttgatg atacgtcgac agtcccgaat cattcaaatt tcattacccc cgctctccct gagcgcactt taccaattgc taggtcaacg gtgagacgtt tgggcggact gtctaaattt ggcgggcctg caaggcgtgt tatattacct caaggaagtg aggatgtcca ggttcaagaa agtgtggaaa gtagcaatgt caatgagctg caaggaaggc acaactctgc gccaatcagg cctccagacg tttattgttc ttatgaccaa gcatccactt caccgactgc aacagccgga tgcgacaatc gattgatgaa catatcacat ccagacgatc gaacagtaaa atcagcgcgc aagccggtaa accaaagcgt acatgaaata aatgaatcaa ttcatgagcc gttggcttta gctactcaac cgcaagggga acatggggta tcacgtatat cggcggcttt atcatcagaa ggagaggaca gactattccg gcctttcggt aaactggaag aaaaattgcg acctgtcgct ggtccgtcga aggcgtcagg ccttaggtat atggattctt tgccggaaac aggaccggaa gctcgggaca tgcagggtaa cagccctcgc tcgtctgatg tcggaaaatc ggccagccct ccctatcgca accttcgtgc tgcccgcccg ccaccggtaa ctgaatcacg cagaaatgcc ccgatggtat caactgctac cgctgttagc gcacctgcgt ctcatcctat gatgtcgtca gagatattgc agcccccgcc cactcaaagc gtgccgcaag gcaagacttt atttcatgtg aacggagtcc catacgaaag attgcaacgc ctgggaaagg gcggttcgtc gacggtttat tctgtccttt actcgggccc taaaaaaaga atcatctatg ctctcaaggt agtgcaactc gaccgtgcag actccgaaac atatcaaagc tacacaaacg aaatcgagct tttgaagcgt ctccgtggac acgatcgtgt tattcagctc attgatcatc aaataacttt caaccaacat aatcgcccac atcgtttgct gatggtcatg gaatgcggcg agattgattt tgcggcgttg ctagatgagc aacgagggaa agcgatcaat atgaattttg tcggtttata ttgggagcag atgcttgaag ctgtccaagc cgtccaccgc gagaacgtcg ttcatacaga tttgaagcca gcgaactttg ttttggtgaa agggcggttg aaaatcattg actttggtat tgcaaaagca atagcaaatg acactgtcaa tattcagcgc gatcagcaaa ttggcaccgt aaactacatg tcacccgagg ctattcaaag aatgaacaat cagaaggtat tgaagttatc ctatcccagc gatgtttggt cacttggttg tatcctttac caaatgattt atggtagtcc cccctttcaa catgttagtg gaggaccatt ggcaaaaatg ggtgtgatcg cagatccgaa ccacgtcgtc acatatccag aagtggctgt acccaaggca gcagttggat tcagtcttga tggtcatcct acagaccccg caagtctgtc tgtccctgtc agcccgtctg ccattgattc aatgaagcga tgcctagcat atcgcaaaga gcatcggctg acaatacctg aattattgca acatgagttt ttgaagccaa gaataagagc tccagctgtt cctccaggat ctactccaat aacacagtat cagatggagc gattggtcaa ctttatacta tcggaaaatg gcctaccgga actgtcagat ggtaatcaca cagctgagga tctattctct cagttggaag ctcaaaacgc attgtctaga tga
Details for CrneC.19443.a.B2.GE41422
HARVESTED ON: 4/5/2017
SEQUENCED ON: 4/27/2017
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHMN GVPYERLQRL GKGGSSTVYS VLYSGPKKRI IYALKVVQLD RADSETYQSY TNEIEPLKRL RGHDRVIQLI DHQITFNQHN RPHRLLMVME CGEIDFAALL DEQRGKAINM NFVGLYWEQM LEAVQAVHRE NVVHTDLKPA NFVLVKGRLK IIDFGIAKAI ANDTVNIQRD QQIGTVNYMS PEAIQRMNNQ KVLKLSYPSD VWSLGCILYQ MIYGSPPFQH VSGGPLAKMG VIADPNHVVT YPEVAVPKAA VGFSLDGHPT DPASLSVPVS PSAIDSMKRC LAYRKEHRLT IPELSQXXFX XAKISE
Validated NT Sequence
atggctcacc accaccacca ccatatgaac ggagtcccat acgaaagatt gcaacgcctg ggaaagggcg gttcgtcgac ggtttattct gtcctttact cgggccctaa aaaaagaatc atctatgctc tcaaggtagt gcaactcgac cgtgcagact ccgaaacata tcaaagctac acaaacgaaa tcgagccttt gaagcgtctc cgtggacacg atcgtgttat tcagctcatt gatcatcaaa taactttcaa ccaacataat cgcccacatc gtttgctgat ggtcatggaa tgcggcgaga ttgattttgc ggcgttgcta gatgagcaac gagggaaagc gatcaatatg aattttgtcg gtttatattg ggagcagatg cttgaagctg tccaagccgt ccaccgcgag aacgtcgttc atacagattt gaagccagcg aactttgttt tggtgaaagg gcggttgaaa atcattgact ttggtattgc aaaagcaata gcaaatgaca ctgtcaatat tcagcgcgat cagcaaattg gcaccgtaaa ctacatgtca cccgaggcta ttcaaagaat gaacaatcag aaggtattga agttatccta tcccagcgat gtttggtcac ttggttgtat cctttaccaa atgatttatg gtagtccccc ctttcaacat gttagtggag gaccattggc aaaaatgggt gtgatcgcag atccgaacca cgtcgtcaca tatccagaag tggctgtacc caaggcagca gttggattca gtcttgatgg tcatcctaca gaccccgcaa gtctgtctgt ccctgtcagc ccgtctgcca ttgattcaat gaagcgatgc ctagcatatc gcaaagagca tcggctgaca atacctgaat tatcgcaaca ngnnttttnn naagccaaga taagtgagta a
Expected Protein Sequence
MAHHHHHHNG VPYERLQRLG KGGSSTVYSV LYSGPKKRII YALKVVQLDR ADSETYQSYT NEIELLKRLR GHDRVIQLID HQITFNQHNR PHRLLMVMEC GEIDFAALLD EQRGKAINMN FVGLYWEQML EAVQAVHREN VVHTDLKPAN FVLVKGRLKI IDFGIAKAIA NDTVNIQRDQ QIGTVNYMSP EAIQRMNNQK VLKLSYPSDV WSLGCILYQM IYGSPPFQHV SGGPLAKMGV IADPNHVVTY PEVAVPKAAV GFSLDGHPTD PASLSVPVSP SAIDSMKRCL AYRKEHRLTI PELLQHEFLK PR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataacggag tcccatacga aagattgcaa cgcctgggaa agggcggttc gtcgacggtt tattctgtcc tttactcggg ccctaaaaaa agaatcatct atgctctcaa ggtagtgcaa ctcgaccgtg cagactccga aacatatcaa agctacacaa acgaaatcga gcttttgaag cgtctccgtg gacacgatcg tgttattcag ctcattgatc atcaaataac tttcaaccaa cataatcgcc cacatcgttt gctgatggtc atggaatgcg gcgagattga ttttgcggcg ttgctagatg agcaacgagg gaaagcgatc aatatgaatt ttgtcggttt atattgggag cagatgcttg aagctgtcca agccgtccac cgcgagaacg tcgttcatac agatttgaag ccagcgaact ttgttttggt gaaagggcgg ttgaaaatca ttgactttgg tattgcaaaa gcaatagcaa atgacactgt caatattcag cgcgatcagc aaattggcac cgtaaactac atgtcacccg aggctattca aagaatgaac aatcagaagg tattgaagtt atcctatccc agcgatgttt ggtcacttgg ttgtatcctt taccaaatga tttatggtag tccccccttt caacatgtta gtggaggacc attggcaaaa atgggtgtga tcgcagatcc gaaccacgtc gtcacatatc cagaagtggc tgtacccaag gcagcagttg gattcagtct tgatggtcat cctacagacc ccgcaagtct gtctgtccct gtcagcccgt ctgccattga ttcaatgaag cgatgcctag catatcgcaa agagcatcgg ctgacaatac ctgaattatt gcaacatgag tttttgaagc caagatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat