CrneC.19441.a

STE/STE11/BCK1 protein kinase

CENTER ID: CrneC.19441.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19441.a.B2.GE41421 ordered domain 1499 1793
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_04755
UniProt: J9VSQ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSHHPQLSVS TGSAGAGPST PTLPRRPQGA RRRGVMPGLF IANPDNSDEE NSPPKAGSQK SPSAVSPISV SSSSGRGGPS SVSIPQPASP SHSIKERGGL PYPSIPAPQS SPLPSISAFP SPQPLVPTPS PAPTAYGHTP EISSGPPQPP PQPQRHLQRA FTTPTPEHTP RLFPEPHPSS TGATSFHQQV PNSPSRALPP LPSPITRQST MPSPVSQSPS TAHGQPFSAP PAYHPHNPTR VASPDDVISP AIGSEHGSIM SVGIGHRNRS GSVQGQQVRL QVTTDNEAFH LVDITGINTA EGIREKVFSK LRIRDEDYPT LSMYRTEIGE PADETPILPA ALLNICRTLG DSRASLKFLV KQTDVPPSSA ASVIPPVSQT DYHPFRAAAD PRRAGIPPIT TDLSNPVLYR VSSRHSKEGS VSSASGEMLN RGELSASDWS DLGHEAEDWS APGLSRSTRK SVLQNAGVRL STSTGGSRSP VTEHSASLGT SSPALPTPPI PPHSTSLQAG EPSIRSSPSS RPSSRHYEFN ELSLPPQFDH FKPSNATSVF PRSGTPINDS YQNENAFSGP SHPAMSQEQG SQGLGLTLDD DIDPETRALI EQFQREEQEA QKKEEERRRQ LEADEELAKR EQQSERELWE MMQQMQKEQR QREQAQIAED EARARQVEEE QRHEEEQRQA LEAARTAWEA EQREQREQRE QREQREQQES RFHTFEKDRR QRQQFFQNRA RMGAPLDDSA TWESTWMVSP STEGSLSSRR PSEGRVPMVQ TSAPRTNQTQ SPALPPRQSS QPIYQPSQSQ YLPDFAYSHR GPSGFPNTSY AETSHAQERL HDPRLQQATG RAPASGQRGR TSNTPRPESQ RIRHPFSYAA HEPSNERLQA PALSTARSVD NLRMTSGPLG VTSRQSQIYG PPRTSITPAP GFAEHRNIQP AATSTSYRSP SSDRARDGRY PEPLHSSSRG ATPMAPAIID TGLVPFPSPH PSSATSSNSW RGYPQQFNPV PRSARGPTFD ITRPNTVHYD RSPPPPTSPE TTIPPRRPST YYDEVHPPDN ARTMFEDGAA VRRPSSPFPW ADRAGSGSAS TPYRTASPVP VEQPRISETN AFDDTDSQLP YAHNASPQSS WLSVSRAVAN RSDSDTLSVA GTVSSEATVR PGKGVGEDND TNSGDTAKAG QWDRHLKEMI AKMGPGSGEG TVRPRKEEEE DEATLFITPP VRAPETSPSR TSAIRPSPSK PNLIVNTVSL SQQAYHQVNP VLSASTPPDS ATESEGTGEG DGEVEGSNIK RGKSFARPRD PNQWNFRPEP EQLYENLDRV FPQIDLDRPI VQGAESIPST PGIESPSRVE IIGGLPLPAG AAPGRQGGPQ AGSGPLGRAP QAPGPSSGPP GTFNRSKFNK AENRRSIRVV ADHKRRTLQR QISQHEDLPP EYDIGAEEVN SAMDDQVVDP RSEEQRKKDN RRSSSMWDHK LVEVTRFARA QANREADIPE SPASDGKPGT VNWVKGELIG KGSYGRVYIA LNVTTGDMMA VKQVELPATE IERHDSRQQG MVKALRDEIE LLKGLEHKNI VAYLGYETSP EYLSIFLEYV PGGTIASIYR APNQARFEPQ LVRFFTEQIL EGLAYLHSKN IWHRDLKGDN ILVDAQGICK ISDFGISKQT ADAYDSFGQA TNMKGSVFWM APEVIHSYSE RSYSGKVDIW SLGCVVLEMW SGKRPWGDME QFAAMFELFN KRSRPPLPPD INLSSVALDF LNEKCLATDP RSRPMARDLL QHEFIKDRDL TWTFKDSKIG KAVAKRGAKR PKA
NT Sequence
atgtctcatc acccgcagct ctcagtttcg actgggtctg ccggtgctgg gccatccact ccgacgctcc ctcgtcgtcc tcaaggtgcg cgtcggcgcg gtgttatgcc tggactattc atcgcaaacc cagacaactc tgatgaggaa aactctccgc ccaaagctgg gagtcaaaaa tcaccgtcag cagtatcacc tatcagtgtc tcatcgagct ccgggagagg tggtcccagt tcagtatcca tccctcagcc agcatcacca tcccatagta tcaaagaacg agggggcttg ccttatcctt caattcctgc acctcaatct tcccctttac cctctatatc ggcgttccca agtccacagc cgttggtacc aacgccatcg cctgcgccaa cagcatatgg tcacactcca gagatatcgt caggaccacc acaaccgccc cctcaaccgc agcggcatct tcaacgggcg tttacaacac caactccgga acatacacct cgactcttcc cagaacctca tccttcaagc acaggtgcaa catcattcca ccaacaagtt cccaactctc catcgcgtgc tcttccccca ttaccatcac ccatcactcg gcagtctaca atgccttccc ccgtttccca atccccgtca accgcgcatg ggcaaccatt ttctgctcct cctgcgtatc atccccataa tccaacaaga gtggcaagtc ccgacgatgt gataagccct gccattggca gcgaacatgg gagcatcatg agtgtgggaa tagggcacag aaatcggtcg ggttcagtgc aaggacaaca ggtcagactg caagtgacga ctgataacga agcgtttcat ctggtagata ttacagggat aaatactgct gaaggtatac gtgagaaagt attctcaaaa ctgcgtatta gagatgaaga ttacccaact ctgtcgatgt atcgtaccga aattggagaa ccggcagatg aaacacctat attacctgct gcacttctca acatttgccg caccctggga gactctcgag ctagcctcaa attcctagtc aagcaaaccg atgttccacc gtcgtccgcc gcatctgtca ttccacccgt ctctcaaacc gattatcatc cttttcgcgc ggcggcggac ccccgacgtg cagggattcc cccaataacg acggatttgt ctaatccagt actttaccgt gtctcaagtc gtcattcaaa agaaggtagt gtaagtagtg ccagcggaga aatgctcaat cggggggagc ttagtgccag tgactggagt gatttaggac atgaagcaga ggactggtca gcgcccggtt tgtcaaggag cacaaggaaa tctgtgttac agaatgcggg tgtgcgacta tcgacttcaa cgggtggatc gagatcacct gttacagaac actcggcttc tttgggtacg agctctccag ctttgcccac accacctata cctcctcatt ccacctcatt acaggctggc gaaccttcca ttcgttcttc cccgtctagt cgaccgtcta gcagacatta cgaatttaac gaactctctt tacctccgca attcgatcac ttcaagcctt ccaatgctac ctctgtcttt cctcgttctg gaacaccaat aaatgactca tatcaaaacg aaaacgcatt ttccggtcct agccatccag caatgagtca agagcaaggc agccaaggac tcgggcttac tttggatgat gacattgacc cagagactcg cgcattgata gagcaattcc aaagggagga acaggaggcg caaaagaagg aggaagagag aagaaggcaa ctcgaagcag atgaagagtt ggcaaagaga gagcagcaga gcgaaagaga actgtgggag atgatgcagc aaatgcaaaa ggagcagagg caaagagaac aagcgcaaat cgcggaagat gaagccagag ctaggcaggt agaggaggag cagagacacg aagaagagca aaggcaagca ctagaagcag cccgtacggc atgggaggcg gagcaacggg aacaaaggga acaacgagag cagcgagagc agcgagagca acaggaaagt cgatttcata catttgagaa agataggcgt cagaggcagc agttctttca gaacagagct aggatggggg ctcctttgga tgactcggcc acatgggagt ctacttggat ggtcagccct tcaacagaag ggtcgctttc ttcaagaagg ccctcagagg gacgagtgcc catggtgcag acatcggcgc cgagaaccaa tcagactcag tctccagcgc taccgccacg gcaaagcagt cagcctattt atcagccatc gcagtcacag tatctgccgg atttcgccta ctctcaccgc ggaccatctg ggtttcccaa tacttcttac gctgaaacct cacatgctca agagcgatta catgaccctc gattgcaaca ggcgacggga cgtgcaccgg cttcggggca gcgcggacgg acatccaata cccctcgccc ggaatctcag cgaattcgac atcctttcag ctacgctgct catgaaccaa gtaatgagcg tcttcaagct cctgcactgt ccactgctag atctgtagat aatttgagga tgaccagcgg gccacttggt gtgacttcac gtcaaagtca gatttacggg cctcccagaa catcaatcac ccctgctcca ggattcgcag agcatcgcaa tattcaacct gcagccacaa gcacgtcgta tcgcagtcca tcatcggaca gagccagaga tgggagatat ccggaacccc tgcattcctc gtctaggggg gcgacaccca tggcaccagc tataattgac acaggtcttg tcccattccc gtcaccgcac ccgagcagcg cgacatcttc gaattcctgg cgaggctacc cgcagcagtt taatcctgta ccaagatcgg ctcgcggtcc aacattcgat atcacacgac ccaacactgt ccattacgac cgctctccgc ctcctcccac ctcacctgaa accacaattc cgcctcgccg accttctacg tattacgatg aagttcaccc tccagacaat gcccgcacta tgttcgaaga tggtgcagct gttcgtcggc catcttcacc attcccttgg gccgatcgag ctggaagcgg tagcgcatcg actccatacc gtactgcatc acctgtcccg gtcgaacaac ctaggatcag tgagacaaat gcatttgacg acaccgactc acagttgccc tatgctcata acgcaagccc acagtcctcg tggttatcag tttccagggc tgttgctaat cggagcgact ctgatacttt gtcagtagct ggcacagtca gcagcgaggc gacagttcgg ccaggaaagg gcgtgggtga agacaacgac acaaactcgg gagacacggc taaggctggg caatgggaca gacatttgaa ggagatgatt gcaaaaatgg gaccaggaag cggagaaggg accgtgagac caaggaagga ggaagaagaa gatgaagcaa cactgtttat cacgccgcct gtccgggcac cagaaacgtc tccatctcgc acaagcgcga tacgcccgtc accttcgaaa cctaatctca tcgtcaacac tgtttctttg agccagcagg cgtatcatca agtcaaccct gtactttcgg cgagcacgcc accagatagc gcaactgagt ctgaaggcac aggtgaaggt gatggtgagg ttgaggggtc caacatcaag cgcggcaaat cctttgctcg ccccagagac cccaaccaat ggaacttccg tccggagccc gagcaactgt atgaaaatct cgacagggtt ttcccgcaga ttgatttgga tagacccatt gtgcaaggag ctgagtcgat accgtcaaca cccgggattg agtcgccgag tcgagtggag atcatcggtg ggctaccatt gcccgcaggt gcagcacctg gtagacaagg tggcccccag gccggatcag gacctttggg tagggcgcct caagcaccag ggccttcttc gggacctcct ggaacgttca atagatcaaa gttcaataaa gcggagaatc gccggtcgat tcgagttgtt gccgatcaca aacgccgcac ccttcagcgt cagatatccc aacacgaaga ccttccacca gaatacgaca tcggtgctga agaggtcaac tctgccatgg atgatcaagt tgtcgacccc agaagtgagg agcagagaaa gaaggacaac agacgaagct caagcatgtg ggatcataag ctggtggaag ttaccaggtt cgcgcgtgct caggccaacc gcgaagccga tatccctgaa tcacctgcct ctgatggcaa gcctggcact gtgaactggg tcaaaggcga gcttatcggg aaaggatctt atggtagggt gtatatcgcg ctcaatgtta caacgggaga tatgatggcg gtcaaacagg tcgaattgcc ggcgacagaa attgaaaggc atgactcaag gcagcaaggg atggtcaaag cgttgaggga tgaaatcgaa ttgctgaagg gtttagagca taagaacatt gtggcgtatt tgggatacga gacaagcccc gagtatttgt ccattttcct tgaatatgtc cccggtggta ccattgcctc aatttaccgc gcccctaacc aagcccgttt cgagccgcag ctcgtcagat tctttactga acagattcta gaagggttag cgtacttgca ttcgaagaat atttggcatc gagatctcaa aggtgataac atcttggtgg atgcgcaggg catatgtaag atctcagact tcggtatttc taagcagaca gccgatgcct atgattcctt tggccaagct accaatatga aggggtccgt cttttggatg gcgcctgagg ttattcattc ttattccgaa cgctcatact ccggaaaggt tgatatctgg agtttgggat gtgttgtgct tgagatgtgg tctggaaaaa ggccttgggg cgacatggag caatttgctg caatgtttga gctcttcaac aaacgatcta gaccgcctct tccgcctgat attaatcttt cttctgttgc tcttgatttc ctcaacgaaa agtgtctcgc tacagaccct cgcagtcggc ccatggcgcg cgaccttctt caacacgagt tcataaaaga tagggacttg acttggactt ttaaagatag caaaattggg aaagctgttg caaagagggg agcgaaaaga cccaaagcat ga
Details for CrneC.19441.a.B2.GE41421
HARVESTED ON: 4/5/2017
SEQUENCED ON: 4/27/2017
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMI GKGSYGRVYI ALNVTTGDMM AVKQVELPAT EIERHDSRQQ GMVKALRDEI ELLKGLEHKN IVAYLGYETS PEYLSIFLEY VPGGTIASIY RAPNQARFEP QLVRFFTEQI LEGLAYLHSK NIWHRDLKGD NILVDAQGIC KISDFGISKQ TADAYDSFGQ ATNMKGSVFW MAPEVIHSYS ERSYSGKVDI WSLGCVVLEM WSGKRPWGDM EQFAAMFELF NKRSRPPLPP DINLSSVALD FLNEKCLATD PRSRPMARDL LQHEFIKDRD LTWTFKDSKI GKAVAKRXXK RPKA
Validated NT Sequence
atggctcacc accaccacca ccatatgatc gggaaaggat cttatggtag ggtgtatatc gcgctcaatg ttacaacggg agatatgatg gcggtcaaac aggtcgaatt gccggcgaca gaaattgaaa ggcatgactc aaggcagcaa gggatggtca aagcgttgag ggatgaaatt gaattgctga agggtttaga gcataagaac attgtggcgt atttgggata cgagacaagc cccgagtatt tgtccatttt ccttgaatat gtccccggtg gtaccattgc ctcaatttac cgcgccccta accaagcccg tttcgagccg cagctcgtca gattctttac tgaacagatt ctagaagggt tagcgtactt gcattcgaag aatatttggc atcgagatct caaaggtgat aacatcttgg tggatgcgca gggcatatgt aagatctcag acttcggtat ttctaagcag acagccgatg cctatgattc ctttggccaa gctaccaata tgaaggggtc cgtcttttgg atggcgcctg aggttattca ttcttattcc gaacgctcat actccggaaa ggttgatatc tggagtttgg gatgtgttgt gcttgagatg tggtctggaa aaaggccttg gggcgacatg gagcaatttg ctgcaatgtt tgagctcttc aacaaacgat ctagaccgcc tcttccgcct gatattaatc tttcttctgt tgctcttgat ttcctcaacg aaaagtgtct cgctacagac cctcgcagtc ggcccatggc gcgcgacctt cttcaacacg agttcataaa agatagggac ttgacttgga cttttaaaga tagcaaaatt gggaaagctg ttgcaaagag gnnnnnnaaa agacccaaag cataagtgag taagat
Expected Protein Sequence
MAHHHHHHIG KGSYGRVYIA LNVTTGDMMA VKQVELPATE IERHDSRQQG MVKALRDEIE LLKGLEHKNI VAYLGYETSP EYLSIFLEYV PGGTIASIYR APNQARFEPQ LVRFFTEQIL EGLAYLHSKN IWHRDLKGDN ILVDAQGICK ISDFGISKQT ADAYDSFGQA TNMKGSVFWM APEVIHSYSE RSYSGKVDIW SLGCVVLEMW SGKRPWGDME QFAAMFELFN KRSRPPLPPD INLSSVALDF LNEKCLATDP RSRPMARDLL QHEFIKDRDL TWTFKDSKIG KAVAKRGAKR PKA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatcggga aaggatctta tggtagggtg tatatcgcgc tcaatgttac aacgggagat atgatggcgg tcaaacaggt cgaattgccg gcgacagaaa ttgaaaggca tgactcaagg cagcaaggga tggtcaaagc gttgagggat gaaatcgaat tgctgaaggg tttagagcat aagaacattg tggcgtattt gggatacgag acaagccccg agtatttgtc cattttcctt gaatatgtcc ccggtggtac cattgcctca atttaccgcg cccctaacca agcccgtttc gagccgcagc tcgtcagatt ctttactgaa cagattctag aagggttagc gtacttgcat tcgaagaata tttggcatcg agatctcaaa ggtgataaca tcttggtgga tgcgcagggc atatgtaaga tctcagactt cggtatttct aagcagacag ccgatgccta tgattccttt ggccaagcta ccaatatgaa ggggtccgtc ttttggatgg cgcctgaggt tattcattct tattccgaac gctcatactc cggaaaggtt gatatctgga gtttgggatg tgttgtgctt gagatgtggt ctggaaaaag gccttggggc gacatggagc aatttgctgc aatgtttgag ctcttcaaca aacgatctag accgcctctt ccgcctgata ttaatctttc ttctgttgct cttgatttcc tcaacgaaaa gtgtctcgct acagaccctc gcagtcggcc catggcgcgc gaccttcttc aacacgagtt cataaaagat agggacttga cttggacttt taaagatagc aaaattggga aagctgttgc aaagagggga gcgaaaagac ccaaagcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat