SSGCID: Target Page

CrneC.19426.a

IRE protein kinase

CENTER ID:	CrneC.19426.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19426.a.B2.GE41414	ordered domain		642	947
CrneC.19426.a.B4.GE41416	N-term domain		45	397

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19426.a.B4.PW38299	N-term domain		45	397

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_03670
UniProt:	J9VN93

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLYFLFLLPL LIAYAAPEPA ALIPFPHHTL RALAPSSTAT LPVQIDHDLH PLVLVSTIDG ALHALERSTG KEKWVLEGDP LVGGKMKGGV EEYIVEPLSG SLYVHEDKDG EMRMRKLPLS VDQLIELSPF TFPESPTQIF TGSKHTSLMS VDLRTGEQVD CFSPTANLSQ YDGSSVCDDL DDLERRGSSQ RDTLFIGRTD YRLTIHSPSS SQGLSTYTSA AYPAEKKSAP AVQEISYSTY TPNAYNRPLA ESWVKAGVAH QSWGPDNEEP RIRIELGFNG KALGVKPGGG LIWNRELEHI GVGVYEILIP RENPLGAPIL VPQPPAHLPA LFPGQNDPRA FNIHDAPPST YIATLPECLT LPSSNTTSGN ATAGNKDTKP LHFALSSSSY PLINFARPPP PGHLSDGLFY NADDGDSSDL DHSRGRGLLS GLIDPPREHE TIDPPFIERQ AGPAKNGWWR WVVALGMLLI LLGGVMVRFG RGKGSVPGSV ESKVDEKMPL LAVPVHEVRL EEKEEEEEED VLPVVKSAPV VRIQEPSSTP EDSPPRSEGT PETHQTATPP PKKKSTRRRV RGKKKKPDAT TTATAAGLTA EERDGENEDE DHEKDKEDFS PRATPKGGNK PLPELPRELS STDLLDYDQD KERLAISDTI IGFGSHGTVV LKGTWGGRPV AVKRLLSDFT RLASQEVKLL QASDDHPNVI RYYCQEKRDN FLYIALDLCQ ASLADLIESP EKHRELADQL DRKRALMEVT KGLKHLHGMK IIHRDIKPQN VLVSQTPSGL RILVSDFGLA RRLGQDQSSF APTANNLAGS LGWRAPECIR GVVRLNEGFD ASSSVGSSGG IANAEDGVAR SRLTKAVDLF ALGCLYFWVL LSGEHPFGET YNRESNIVKG EAVNMGMLSL LGEEREEVED LVKMLLSTEP DARPSTSECL THPIFWPAAK RLGFLCDASD RFEIMQTEPA EPTLVLLEQG AQSVVGKDWY SRLDKTFTGS LGKYRKYKGG SVRDMLRAMR NKKHHYQDLE PAVQKHLGAL PAGFLLYFSS RYPKLLMHVY RTVKESELRE ESMFEGCFQE AV

NT Sequence

atgctctact tcttgttcct cctccccctc ctcatcgcct acgccgctcc agagccagca gccctcatcc ctttcccaca tcacaccctt cgagcccttg ccccctcctc cactgccaca ctccccgtcc agatagacca tgacctgcac ccgcttgtcc tcgtgtccac catagacggt gctttgcatg ccctggagag aagcacgggt aaagagaaat gggtactcga aggtgacccg ctggtcggtg ggaagatgaa aggcggtgtg gaagagtaca ttgtggaacc cctgagtggg agtctgtatg tgcacgagga caaggatggg gagatgagga tgagaaagct gcctctttct gtagatcaat taattgagct atccccgttt acattccccg aatctccgac ccaaatcttt accggtagca aacacacttc tctaatgagt gttgatctgc gaactgggga acaggttgat tgcttttctc ctactgccaa tctatcgcaa tatgacggct caagcgtgtg cgacgacctg gacgatctcg agcgacgcgg ctcttcacag cgagacacat tgttcatcgg tcgaacagat tatcgactta cgatccattc gccatcttct tcccaaggtt tatctacgta tacatccgcg gcttatccgg cggaaaaaaa atcggctccc gccgtccaag agatctctta ctccacctac acccctaatg cttataaccg cccacttgca gaatcatggg tcaaggcggg tgtggcacac caatcatggg gtcccgacaa tgaagagcct cggatacgta tcgagcttgg attcaatggc aaggcacttg gtgtaaagcc tggtggtgga ctcatttgga atcgagagct ggaacatata ggcgtcggcg tgtacgagat cctcattcct cgagaaaatc ccttgggcgc acccattctt gtcccgcaac ctcctgccca ccttcctgct ctcttccccg gacaaaatga cccgagagca tttaatattc acgacgcacc tccttccacc tatatcgcta cactccccga atgtctcact ttgccgtcat ccaatacaac ttccggcaac gcgacggcag gcaacaaaga caccaagccg ttacatttcg cgctttcatc ttcttcatat cctcttatca actttgcccg tccccctcct cctggacatc tctccgatgg attattctac aatgccgacg atggcgattc ttctgatctc gaccatagtc gtggtagggg gcttttatca gggttgattg acccacccag ggagcacgag acgattgatc cgccattcat tgaaagacag gctggacctg caaagaatgg gtggtggaga tgggtggtgg cattgggaat gttattgatc ctgttgggtg gtgtgatggt ccgcttcgga aggggaaaag gctcggtgcc aggatcagtg gaaagcaagg tagatgagaa aatgccttta ctcgctgtac ctgtccacga ggtgagactt gaagaaaaag aagaagagga agaagaagat gtactgccag tagtcaagtc tgcccctgtg gtgcgcatcc aagaaccatc gtctacgcct gaagactccc ccccacgctc ggaaggcaca cccgaaacac atcagaccgc cacgcctcca ccaaagaaaa agtctactcg tcgtcgtgtg cggggtaaaa agaagaagcc tgatgctact actacagcta ccgcggccgg tctgacggcc gaagagcgcg atggtgagaa tgaagatgag gatcatgaaa aggacaagga agacttttct ccccgggcga cgcccaaggg aggtaataaa cccttgccag aattgccgag agaactttct tcgacagatt tgttggatta tgatcaggat aaggagcggc tggcgatctc agatactatc attggttttg gatcgcatgg tactgtagtg ttaaagggaa catggggagg acgcccagtt gcagtaaaac gactgttgag cgactttacc agattggcga gtcaagaagt caagttgttg caagcaagcg acgatcatcc caatgtcatc cgctactact gccaagaaaa gcgcgacaat ttcctctaca tcgccctcga cctgtgtcaa gcatccttgg cagatttgat cgaatcacct gaaaagcata gagagttggc agatcagctg gataggaagc gggcgttgat ggaggttaca aagggtttga aacatttgca tgggatgaag attattcatc gggacattaa accgcaaaac gtgctcgtat cccaaacacc ttctgggctg agaatcctcg tttccgactt tggacttgcc cgccgactag gccaagatca atcatccttt gcgccgacgg cgaacaactt ggccggtagc ctaggctggc gagcacccga atgtatccga ggtgtggtcc gtctcaacga agggtttgac gcttcgtcct ctgtcggatc ttctggcggt atcgccaatg cagaagatgg cgttgcgcga agtcgattga ccaaggctgt ggatctgttt gcgcttggat gcttatactt ttgggtcctg ctttccgggg aacatccctt tggcgagacg tataatcggg agagtaatat agtcaagggt gaagctgtga atatgggtat gttgagttta ttaggggagg aaagagaaga agtggaagat ttggtgaaga tgttgttgag taccgagccc gacgcaagac catccacatc agaatgtctc acccatccca tcttctggcc cgccgccaaa cgtctcggct ttctgtgtga cgcttccgac cgattcgaaa tcatgcaaac cgagccggcc gaacctacac ttgtacttct cgaacaaggc gcacaaagtg tcgtcggcaa ggattggtat tcccggttgg ataagacgtt tacaggcagt ttgggcaagt atagaaagta taaaggcggg agtgtgaggg atatgttgag ggctatgcgg aataagaaac atcattatca agatctggaa ccggcagtgc aaaagcatct gggcgccctt cctgccggat tcttacttta tttttcctcg cgatacccaa agctgttaat gcacgtgtat cgtacggtaa aggagagtga gttgagggag gaaagtatgt ttgaagggtg tttccaagag gcggtgtaa

Details for CrneC.19426.a.B2.GE41414

HARVESTED ON:	4/5/2017
SEQUENCED ON:	4/27/2017
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHER LAISDTIIGF GSHGTVVLKG TWGGRPVAVK RLLSDFTRLA SQEVKLLQAS DDHPNVIRYY CQEKRDNFLY IALDLCQASL ADLIESPEKH RELADQLDRK RALMEVTKGL KHLHGMKIIH RDIKPQNVLV SQTPSGLRIL VSDFGLARRL GQDQSSFAPT ANNLAGSLGW RAPECIRGVV RLNEGFDASS SVGSSGGIAN AEDGVARSRL TKAVDLFALG CLYFWVLLSG EHPFGETYNR ESNIVKGEAV NMGMLSLLGE EREEVEDLVK MLLSTEPDAR PSTSECLTHP IFWPAAKRLG FLCD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgagcggc tggcgatctc agatactatc attggttttg gatcgcatgg tactgtagtg ttaaagggaa catggggagg acgcccagtt gcagtaaaac gactgttgag cgactttacc agattggcga gtcaagaagt caagttgttg caagcaagcg acgatcatcc caatgtcatc cgctactact gccaagaaaa gcgcgacaat ttcctctaca tcgccctcga cctgtgtcaa gcatccttgg cagatttgat cgaatcacct gaaaagcata gagagttggc agatcagctg gataggaagc gggcgttgat ggaggttaca aagggtttga aacatttgca tgggatgaag attattcatc gggacattaa accgcaaaac gtgctcgtat cccaaacacc ttctgggctg agaatcctcg tttccgactt tggacttgcc cgccgactag gccaagatca atcatccttt gcgccgacgg cgaacaactt ggccggtagc ctaggctggc gagcacccga atgtatccga ggtgtggtcc gtctcaacga agggtttgac gcttcgtcct ctgtcggatc ttctggcggt atcgccaatg cagaagatgg cgttgcgcga agtcgattga ccaaggctgt ggatctgttt gcgcttggat gcttatactt ttgggtcctg ctttccgggg aacatccctt tggcgagacg tataatcggg agagtaatat agtcaagggt gaagctgtga atatgggtat gttgagttta ttaggggagg aaagagaaga agtggaagat ttggtgaaga tgttgttgag taccgagccc gacgcaagac catccacatc agaatgtctc acccatccca tcttctggcc cgccgccaaa cgtctcggct ttctgtgtga ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for CrneC.19426.a.B4.GE41416

HARVESTED ON:	4/5/2017
SEQUENCED ON:	4/27/2017
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMI DHDLHPLVLV STIDGALHAL ERSTGKEKWV LEGDPLVGGK MKGGVEEYIV EPLSGSLYVH EDKDGEMRMR KLPLSVDQLI ELSPFTFPES PTQIFTGSKH TSLMSVDLRT GEQVDCFSPT ANLSQYDGSS VCDDLDDLER RGSSQRDTLF IGRTDYRLTI HSPSSSQGLS TYTSAAYPAE KKSAPAVQEI SYSTYTPNAY NRPLAESWVK AGVAHQSWGP DNEEPRIRIE LGFNGKALGV KPGGGLIWNR ELEHIGVGVY EILIPRENPL GAPILVPQPP AHLPALFPGQ NDPRAFNIHD APPSTYIATL PECLTLPSSN TTSGNATAGN KXXKPLHFAL SS

Validated NT Sequence

atggctcacc accaccacca ccatatgata gaccatgacc tgcacccgct tgtcctcgtg tccaccatag acggtgcttt gcatgccctg gagagaagca cgggtaaaga gaaatgggta ctcgaaggtg acccgctggt cggtgggaag atgaaaggcg gtgtggaaga gtacattgtg gaacccctga gtgggagtct gtatgtgcac gaggacaagg atggggagat gaggatgaga aagctgcctc tttctgtaga tcaattaatt gagctatccc cgtttacatt ccccgaatct ccgacccaaa tctttaccgg tagcaaacac acttctctaa tgagtgttga tctgcgaact ggggaacagg ttgattgctt ttctcctact gccaatctat cgcaatatga cggctcaagc gtgtgcgacg acctggacga tctcgagcga cgcggctctt cacagcgaga cacattgttc atcggtcgaa cagattatcg acttacgatc cattcgccat cttcttccca aggtttatct acgtatacat ccgcggctta tccggcggaa aaaaaatcgg ctcccgccgt ccaagagatc tcttactcca cctacacccc taatgcttat aaccgcccac ttgcagaatc atgggtcaag gcgggtgtgg cacaccaatc atggggtccc gacaatgaag agcctcggat acgtatcgag cttggattca atggcaaggc acttggtgta aagcctggtg gtggactcat ttggaatcga gagctggaac atataggcgt cggcgtgtac gagatcctca ttcctcgaga aaatcccttg ggcgcaccca ttcttgtccc gcaacctcct gcccaccttc ctgctctctt ccccggacaa aatgacccga gagcatttaa tattcacgac gcacctcctt ccacctatat cgctacactc cccgaatgtc tcactttgcc gtcatccaat acaacttccg gcaacgcgac ggcaggcaac aaagnnnnna agccgttaca tttcgcgctt tcatcttc

Expected Protein Sequence

MAHHHHHHID HDLHPLVLVS TIDGALHALE RSTGKEKWVL EGDPLVGGKM KGGVEEYIVE PLSGSLYVHE DKDGEMRMRK LPLSVDQLIE LSPFTFPESP TQIFTGSKHT SLMSVDLRTG EQVDCFSPTA NLSQYDGSSV CDDLDDLERR GSSQRDTLFI GRTDYRLTIH SPSSSQGLST YTSAAYPAEK KSAPAVQEIS YSTYTPNAYN RPLAESWVKA GVAHQSWGPD NEEPRIRIEL GFNGKALGVK PGGGLIWNRE LEHIGVGVYE ILIPRENPLG APILVPQPPA HLPALFPGQN DPRAFNIHDA PPSTYIATLP ECLTLPSSNT TSGNATAGNK DTKPLHFALS SSSYPLINFA R

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatagacc atgacctgca cccgcttgtc ctcgtgtcca ccatagacgg tgctttgcat gccctggaga gaagcacggg taaagagaaa tgggtactcg aaggtgaccc gctggtcggt gggaagatga aaggcggtgt ggaagagtac attgtggaac ccctgagtgg gagtctgtat gtgcacgagg acaaggatgg ggagatgagg atgagaaagc tgcctctttc tgtagatcaa ttaattgagc tatccccgtt tacattcccc gaatctccga cccaaatctt taccggtagc aaacacactt ctctaatgag tgttgatctg cgaactgggg aacaggttga ttgcttttct cctactgcca atctatcgca atatgacggc tcaagcgtgt gcgacgacct ggacgatctc gagcgacgcg gctcttcaca gcgagacaca ttgttcatcg gtcgaacaga ttatcgactt acgatccatt cgccatcttc ttcccaaggt ttatctacgt atacatccgc ggcttatccg gcggaaaaaa aatcggctcc cgccgtccaa gagatctctt actccaccta cacccctaat gcttataacc gcccacttgc agaatcatgg gtcaaggcgg gtgtggcaca ccaatcatgg ggtcccgaca atgaagagcc tcggatacgt atcgagcttg gattcaatgg caaggcactt ggtgtaaagc ctggtggtgg actcatttgg aatcgagagc tggaacatat aggcgtcggc gtgtacgaga tcctcattcc tcgagaaaat cccttgggcg cacccattct tgtcccgcaa cctcctgccc accttcctgc tctcttcccc ggacaaaatg acccgagagc atttaatatt cacgacgcac ctccttccac ctatatcgct acactccccg aatgtctcac tttgccgtca tccaatacaa cttccggcaa cgcgacggca ggcaacaaag acaccaagcc gttacatttc gcgctttcat cttcttcata tcctcttatc aactttgccc gttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for CrneC.19426.a.B4.PW38299

PURIFICATION DATe:	6/12/2017
CONCENTRATION:	24.5mg/ml
OBSERVED MW:	43kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATAGACC ATGACCTGCA CCCGCTTGTC CTCGTGTCCA CCATAGACGG TGCTTTGCAT GCCCTGGAGA GAAGCACGGG TAAAGAGAAA TGGGTACTCG AAGGTGACCC GCTGGTCGGT GGGAAGATGA AAGGCGGTGT GGAAGAGTAC ATTGTGGAAC CCCTGAGTGG GAGTCTGTAT GTGCACGAGG ACAAGGATGG GGAGATGAGG ATGAGAAAGC TGCCTCTTTC TGTAGATCAA TTAATTGAGC TATCCCCGTT TACATTCCCC GAATCTCCGA CCCAAATCTT TACCGGTAGC AAACACACTT CTCTAATGAG TGTTGATCTG CGAACTGGGG AACAGGTTGA TTGCTTTTCT CCTACTGCCA ATCTATCGCA ATATGACGGC TCAAGCGTGT GCGACGACCT GGACGATCTC GAGCGACGCG GCTCTTCACA GCGAGACACA TTGTTCATCG GTCGAACAGA TTATCGACTT ACGATCCATT CGCCATCTTC TTCCCAAGGT TTATCTACGT ATACATCCGC GGCTTATCCG GCGGAAAAAA AATCGGCTCC CGCCGTCCAA GAGATCTCTT ACTCCACCTA CACCCCTAAT GCTTATAACC GCCCACTTGC AGAATCATGG GTCAAGGCGG GTGTGGCACA CCAATCATGG GGTCCCGACA ATGAAGAGCC TCGGATACGT ATCGAGCTTG GATTCAATGG CAAGGCACTT GGTGTAAAGC CTGGTGGTGG ACTCATTTGG AATCGAGAGC TGGAACATAT AGGCGTCGGC GTGTACGAGA TCCTCATTCC TCGAGAAAAT CCCTTGGGCG CACCCATTCT TGTCCCGCAA CCTCCTGCCC ACCTTCCTGC TCTCTTCCCC GGACAAAATG ACCCGAGAGC ATTTAATATT CACGACGCAC CTCCTTCCAC CTATATCGCT ACACTCCCCG AATGTCTCAC TTTGCCGTCA TCCAATACAA CTTCCGGCAA CGCGACGGCA GGCAACAAAG ACACCAAGCC GTTACATTTC GCGCTTTCAT CTTCTTCATA TCCTCTTATC AACTTTGCCC GTTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT