CrneC.19405.a

PfkB family carbohydrate kinase superfamily

CENTER ID: CrneC.19405.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19405.a.B2.GE42595 PfkB_kinase domain 54 425
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_06033
UniProt: J9VUW8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSPYIRPSEA RTLGESARCA TRLPPILASI GTVLIDAFDS LPQPTVDDSP ATSSRPASPV INEQAPQPQG HLQALVVPSR RLRLSPPLSS PAASGTLGPS TPQMTLDDIP VPNAEEIYEM LGGGALYALV GARFWLPPCQ LRTLVDRAPA ENDDCPKDVE QKLAKLGNEI WVWNRGEGTR MTRARIRYEG DVRYFQPVVK APYRTIQELF TSPLLHAEYL HISPPYSPEN VAVIISDLKA LPKDSWRPKI VFEPTPPSCH PGQKDWLEKI LPDIEVFSPN HEELFSFYSI PTMSTSSISL HPSVERLVTH ILHNVGIGAN GQGIVVVRCG RLGACVGTKE GGLKWCPAYW EGGDVRKVKD VTGAGNSFLG GYVAGLSLTN DPYEALLYGT VSSSFVVEQF GLPLLMDCTD PLTGEEIWNA DIPSRRLKEL KQRLGLI
NT Sequence
atgagcccct acatccgtcc atccgaagca aggaccctcg gggagtctgc caggtgcgcc acacgactac cccccattct ggcgtccatc ggaaccgtcc tgattgatgc attcgacagt ttacctcagc ctactgtaga tgatagcccg gctacttctt ctcgaccagc gtccccagtc atcaacgagc aggcgcctca accgcagggg catcttcaag ccttagttgt tccttcgcgt cgccttcgcc tttcccctcc cctgtcgtcc ccagcggcgt ccggaacctt agggccttcg actccccaga tgacccttga tgatatcccc gttccaaatg cggaagagat ttatgaaatg ctgggcggtg gtgctctata tgcactcgtc ggggcaagat tttggcttcc tccttgccag ttacgaactc tcgtcgatcg agccccggct gaaaatgatg actgcccgaa agatgtggaa caaaagctgg cgaaattggg taatgaaata tgggtttgga atagaggtga gggaacgaga atgacaagag caagaatacg gtatgagggc gatgtccggt acttccaacc cgttgtgaag gctccatatc ggacaataca ggagcttttt acttcacctc ttctccatgc cgaatatctt cacatttccc ctccttattc tccagaaaat gtggccgtta tcatctctga tctgaaagct ttgccaaaag atagctggcg acctaagatt gtctttgagc ctactccccc ctcatgccac cctggccaga aggactggct cgaaaagatt cttccggata tcgaagtatt ctcccccaat cacgaagagc ttttttcttt ctattctatc cctaccatgt caacctcttc tatctcgttg catccctcag ttgaacgcct ggtgacccat attctgcaca atgtcggtat tggcgcgaat gggcaaggta tagtggtcgt taggtgtggt cggctaggag catgtgtggg caccaaggaa ggtggattaa aatggtgtcc ggcttattgg gaaggtggtg atgtgaggaa agtaaaagat gtgactggag ctggcaactc tttcttggga ggttatgtag caggcctttc cctaactaat gacccttatg aagctctttt atacggcacc gtttcatcct ccttcgttgt agagcagttc ggactgcctc ttttaatgga ttgcaccgat cctctgacgg gtgaagaaat ttggaatgcc gacataccct ctcgccgatt gaaggaactg aaacagcgct tgggtctaat ataa
Details for CrneC.19405.a.B2.GE42595
HARVESTED ON: 8/5/2018
SEQUENCED ON: 8/8/2018
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS RPASPVINEQ APQPQGHLQA LVVPSRRLRL SPPLSSPAAS GTLGPSTPQM TLDDIPVPNA EEIYEMLGGG ALYALVGARF WLPPCQLRTL VDRAPAENDD CPKDVEQKLA KLGNEIWVWN RGEGTRMTRA RIRYEGDVRY FQPVVKAPYR TIQELFTSPL LHAEYLHISP PYSPENVAVI ISDLKALPKD SWRPKIVFEP TPPSCHPGQK DWLEKILPDI EVFSPNHEKL FSFYSIPTMS TSSISLHPSV ERLVTHILHN VGIGANGQGI VVVRCGRLGA CVGTKEGGLK WCPAYWEGGD VRKVKDVTGA GNSFLGGYVA GLSLTNDPYE ALLYGTVSSS FVVEQFGLPL LMDCTDPLTG EE
Validated NT Sequence
tttcttcacc cgtcagagga tcggtgcaat ccattaaaag aggcagtccg aactgctcta caacgaagga ggatgaaacg gtgccgtata aaagagcttc ataagggtca ttagttaggg aaaggcctgc tacataacct cccaagaaag agttgccagc tccagtcaca tcttttactt tcctcacatc accaccttcc caataagccg gacaccattt taatccacct tccttggtgc ccacacatgc tcctagccga ccacacctaa cgaccactat accttgccca ttcgcgccaa taccgacatt gtgcagaata tgggtcacca ggcgttcaac tgagggatgc aacgagatag aagaggttga catggtaggg atagaataga aagaaaaaag cttttcgtga ttgggggaga atacttcgat atccggaaga atcttttcga gccagtcctt ctggccaggg tggcatgagg ggggagtagg ctcaaagaca atcttaggtc gccagctatc ttttggcaaa gctttcagat cagagatgat aacggccaca ttttctggag aataaggagg ggaaatgtga agatattcgg catggagaag aggtgaagta aaaagctcct gtattgtccg atatggagcc ttcacaacgg gttggaagta ccggacatcg ccctcatacc gtattcttgc tcttgtcatt ctcgttccct cacctctatt ccaaacccat atttcattac ccaatttcgc cagcttttgt tccacatctt tcgggcagtc atcattttca gccggggctc gatcgacgag agttcgtaac tggcaaggag gaagccaaaa tcttgccccg acgagtgcat atagagcacc accgcccagc atttcataaa tctcttccgc atttggaacg gggatatcat caagggtcat ctggggagtc gaaggcccta aggttccgga cgccgctggg gacgacaggg gaggggaaag gcgaaggcga cgcgaaggaa caactaaggc ttgaagatgc ccctgcggtt gaggcgcctg ctcgttgatg actggggacg ctggtcgaga catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHSR PASPVINEQA PQPQGHLQAL VVPSRRLRLS PPLSSPAASG TLGPSTPQMT LDDIPVPNAE EIYEMLGGGA LYALVGARFW LPPCQLRTLV DRAPAENDDC PKDVEQKLAK LGNEIWVWNR GEGTRMTRAR IRYEGDVRYF QPVVKAPYRT IQELFTSPLL HAEYLHISPP YSPENVAVII SDLKALPKDS WRPKIVFEPT PPSCHPGQKD WLEKILPDIE VFSPNHEELF SFYSIPTMST SSISLHPSVE RLVTHILHNV GIGANGQGIV VVRCGRLGAC VGTKEGGLKW CPAYWEGGDV RKVKDVTGAG NSFLGGYVAG LSLTNDPYEA LLYGTVSSSF VVEQFGLPLL MDCTDPLTGE EIWNADIPSR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctcgac cagcgtcccc agtcatcaac gagcaggcgc ctcaaccgca ggggcatctt caagccttag ttgttccttc gcgtcgcctt cgcctttccc ctcccctgtc gtccccagcg gcgtccggaa ccttagggcc ttcgactccc cagatgaccc ttgatgatat ccccgttcca aatgcggaag agatttatga aatgctgggc ggtggtgctc tatatgcact cgtcggggca agattttggc ttcctccttg ccagttacga actctcgtcg atcgagcccc ggctgaaaat gatgactgcc cgaaagatgt ggaacaaaag ctggcgaaat tgggtaatga aatatgggtt tggaatagag gtgagggaac gagaatgaca agagcaagaa tacggtatga gggcgatgtc cggtacttcc aacccgttgt gaaggctcca tatcggacaa tacaggagct ttttacttca cctcttctcc atgccgaata tcttcacatt tcccctcctt attctccaga aaatgtggcc gttatcatct ctgatctgaa agctttgcca aaagatagct ggcgacctaa gattgtcttt gagcctactc ccccctcatg ccaccctggc cagaaggact ggctcgaaaa gattcttccg gatatcgaag tattctcccc caatcacgaa gagctttttt ctttctattc tatccctacc atgtcaacct cttctatctc gttgcatccc tcagttgaac gcctggtgac ccatattctg cacaatgtcg gtattggcgc gaatgggcaa ggtatagtgg tcgttaggtg tggtcggcta ggagcatgtg tgggcaccaa ggaaggtgga ttaaaatggt gtccggctta ttgggaaggt ggtgatgtga ggaaagtaaa agatgtgact ggagctggca actctttctt gggaggttat gtagcaggcc tttccctaac taatgaccct tatgaagctc ttttatacgg caccgtttca tcctccttcg ttgtagagca gttcggactg cctcttttaa tggattgcac cgatcctctg acgggtgaag aaatttggaa tgccgacata ccctctcgct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat