SSGCID: Target Page

CrneC.19400.a

Hydroxyethylthiazole kinase

CENTER ID:	CrneC.19400.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19400.a.B2.GE42590	TMP/TenI domain		7	223
CrneC.19400.a.B3.GE42591	Hyethyz_kinase domain		261	537

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_04230
UniProt:	J9VR92

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPKPTLDYSL YLVTGRELLP PGKDYYESLE ESLQGGVTLV QVREKYADTG EFIEIARRTK TICDKYNVPV LINDRIDVHL AVGTAGIHVG QTDCPIGLAR SLVGPDAIIG LSVGNVNEAK RAIQQGADYV GIGAVWPTNS KDVTKKKMLG PDGVGEILDL LHGTGVQSVA IGGIHLPNVA QLLHASIAPQ SRNTLAGIAI ISDIVASPTP REAAKNLREV VQSFKRARSQ LANLEAVYGA NLFSSPRSVD GFIKEAVHLI DVIKRETPLV NQMTNNVVIN DSANVTLAIG ASPIMATHPR DVHDLSPAIG ALLINFGTIT DKAGMLVAGR QANINRKPII FDPVAIGATP YRQETSVELL AHWQPTIIKG NAGEIGFMAK STEVASRGVD SVGSGFSDPG AVVKALARKQ AAIIVLTGEH DYISDGSTTL KISNGHHYLE RITGSGCQLG SVIASFAATA RLEHLAKHGE WENASQLVQG DMLAAAVTGV LVYTIAAEVA AAREDVKGPG TFRAALIDEL YNLNPEVLRQ RAKIEIL

NT Sequence

atgcccaaac ccactctcga ctactccctc taccttgtca ccgggaggga gcttttgcct cctggaaagg actactacga aagtcttgaa gaatcccttc aaggcggcgt tactctggta caggtccgtg agaagtacgc cgacactgga gaatttattg aaatagctcg ccgcacaaaa accatctgtg ataagtacaa cgtcccagtc cttatcaacg accgtatcga cgtccatctc gctgtcggta ctgcgggcat tcacgttggt caaaccgact gccctatcgg tttggctcgt agccttgtcg gacccgatgc cattattggt ttgtctgtcg gcaacgtcaa cgaagccaaa cgtgccatcc agcaaggtgc agactatgtc ggcatcggtg ctgtgtggcc taccaacagc aaggatgtga ccaagaagaa gatgctgggc cccgatggag tgggcgagat tcttgatttg ctccatggaa ctggcgtaca gagcgtcgct atcggcggca ttcaccttcc caacgtcgct cagctcctcc atgcttccat tgcaccccaa tcacgcaata ctcttgctgg cattgccatc atctccgaca tcgtcgcctc tcccactcct cgcgaggctg ccaagaacct acgagaggtt gttcaatctt ttaagcgcgc aaggagtcag ctcgccaatc ttgaagctgt atacggcgcc aacctgttca gcagcccaag gagcgtagat ggttttatta aggaggctgt ccacttgatt gatgtgatta agagggagac tccattggtc aaccaaatga ctaacaacgt cgtcattaac gattctgcca atgttacctt ggccattggt gcctctccta tcatggcgac ccatcctcgt gacgtccatg atctcagtcc cgccatcgga gctctcttga tcaattttgg caccatcaca gacaaagcag gcatgcttgt ggctggccga caggccaaca ttaacaggaa acccatcatt tttgaccctg tggccatcgg cgcaactcca taccggcaag aaacgtctgt cgagcttctt gctcactggc agccgacaat tatcaagggt aacgctggtg aaatcggatt catggcgaaa tcgacagaag tcgccagtcg aggtgttgat tccgttggtt ctgggttctc tgatccaggt gctgtcgtca aagcactcgc tcgaaagcag gccgcaatta tcgtcctgac aggcgaacat gactatatct cagatggttc aaccactctc aaaatctcca atggtcacca ctacctagaa cgtatcactg gttctgggtg ccagcttgga tcggttattg catcatttgc agctaccgca agactggagc atctggcaaa acatggtgaa tgggagaatg cgtcgcagct cgttcaaggt gacatgttgg ctgcggctgt gaccggtgtg ttggtgtata ccattgccgc ggaggtggcg gccgctcgtg aggatgtaaa agggcctggg acgttcagag ccgccttgat tgatgaattg tacaacctca accccgaggt tttgcgacag cgagccaaga ttgagatctt gtag

Details for CrneC.19400.a.B2.GE42590

HARVESTED ON:	data unavailable
SEQUENCED ON:	8/8/2018
EXPECTED MW:	24kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD YSLYLVTGRE LLPPGKDYYE SLEESLQGGV TLVQVREKYA DTGEFIEIAR RTKTICDKYN VPVLINDRID VHLAVGTAGI HVGQTDCPIG LARSLVGPDA IIGLSVGNVN EAKRAIQQGA DYVGIGAVWP TNSKDVTKKK MLGPDGVGEI LDLLHGTGVQ SVAIGGIHLP NVAQLLHASI APQSRNTLAG IAIISDIVAS PTPREAAKNL REVVQS

Validated NT Sequence

atcttactca cttaagattg aacaacctct cgtaggttct tggcagcctc gcgaggagtg ggagaggcga cgatgtcgga gatgatggca atgccagcaa gagtattgcg tgattggggt gcaatggaag catggaggag ctgagcgacg ttgggaaggt gaatgccgcc gatagcgacg ctctgtacgc cagttccatg gagcaaatca agaatctcgc ccactccatc ggggcccagc atcttcttct tggtcacatc cttgctgttg gtaggccaca cagcaccgat gccgacatag tctgcacctt gctggatggc acgtttggct tcgttgacgt tgccgacaga caaaccaata atggcatcgg gtccgacaag gctacgagcc aaaccgatag ggcagtcggt ttgaccaacg tgaatgcccg cagtaccgac agcgagatgg acgtcgatac ggtcgttgat aaggactggg acgttgtact tatcacagat ggtttttgtg cggcgagcta tttcaataaa ttctccagtg tcggcgtact tctcacggac ctgtaccaga gtaacgccgc cttgaaggga ttcttcaaga ctttcgtagt agtcctttcc aggaggcaaa agctccctcc cggtgacaag gtagagggag tagtccatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHDY SLYLVTGREL LPPGKDYYES LEESLQGGVT LVQVREKYAD TGEFIEIARR TKTICDKYNV PVLINDRIDV HLAVGTAGIH VGQTDCPIGL ARSLVGPDAI IGLSVGNVNE AKRAIQQGAD YVGIGAVWPT NSKDVTKKKM LGPDGVGEIL DLLHGTGVQS VAIGGIHLPN VAQLLHASIA PQSRNTLAGI AIISDIVASP TPREAAKNLR EVVQS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgactact ccctctacct tgtcaccggg agggagcttt tgcctcctgg aaaggactac tacgaaagtc ttgaagaatc ccttcaaggc ggcgttactc tggtacaggt ccgtgagaag tacgccgaca ctggagaatt tattgaaata gctcgccgca caaaaaccat ctgtgataag tacaacgtcc cagtccttat caacgaccgt atcgacgtcc atctcgctgt cggtactgcg ggcattcacg ttggtcaaac cgactgccct atcggtttgg ctcgtagcct tgtcggaccc gatgccatta ttggtttgtc tgtcggcaac gtcaacgaag ccaaacgtgc catccagcaa ggtgcagact atgtcggcat cggtgctgtg tggcctacca acagcaagga tgtgaccaag aagaagatgc tgggccccga tggagtgggc gagattcttg atttgctcca tggaactggc gtacagagcg tcgctatcgg cggcattcac cttcccaacg tcgctcagct cctccatgct tccattgcac cccaatcacg caatactctt gctggcattg ccatcatctc cgacatcgtc gcctctccca ctcctcgcga ggctgccaag aacctacgag aggttgttca atcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for CrneC.19400.a.B3.GE42591

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	81
PERCENT COVERAGE:	8

Validated AA Sequence

MAHHHHHHHM DVIKRETPIG QPND

Validated NT Sequence

ttactcactt acaagatctc aatnnnnnnn nnnnnnnnnn nnaacctcgg ggttgaggtt gtacaattca tcaatcaagg cggctctgaa cgtcccaggc ccttttacat cctcacgagc ggccgccacc tccgcggcaa tggtatacac caacacaccg gtcacagccg cagccaacat gtcaccttga acgagctgcg acgcattctc ccattcacca tgttttgcca gatgctccag tcttgcggta gctgcaaatg atgcaataac cgatccaagc tggcacccag aaccagtgat acgttctagg tagtggtgac cattggagat tttgagagtg gttgaaccat ctgagatata gtcatgttcg cctgtcagga cgataattgc ggcctgcttt cgagcgagtg ctttgacgac agcacctgga tcagagaacc cagaaccaac ggaatcaaca cctcgactgg cgacttctgt cgatttcgcc atgaatccga tttcaccagc gttacccttg ataattgtcg gctgccagtg agcaagaagc tcgacagacg tttcttgccg gtatggagtt gcgccgatgg ccacagggtc aaaaatgatg ggtttcctgt taatgttggc ctgtcggcca gccacaagca tgcctgcttt gtctgtgatg gtgccaaaat tgatcaagag agctccgatg gcgggactga gatcatggac gtcacgagga tgggtcgcca tgataggaga ggcaccaatg gccaaggtaa cattggcaga atcgttaatg acgacgttgt tagtcatttg gttgaccaat gggagtctcc ctcttaatca catccatatg gtggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHDV IKRETPLVNQ MTNNVVINDS ANVTLAIGAS PIMATHPRDV HDLSPAIGAL LINFGTITDK AGMLVAGRQA NINRKPIIFD PVAIGATPYR QETSVELLAH WQPTIIKGNA GEIGFMAKST EVASRGVDSV GSGFSDPGAV VKALARKQAA IIVLTGEHDY ISDGSTTLKI SNGHHYLERI TGSGCQLGSV IASFAATARL EHLAKHGEWE NASQLVQGDM LAAAVTGVLV YTIAAEVAAA REDVKGPGTF RAALIDELYN LNPEVLRQRA KIEIL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgatgtga ttaagaggga gactccattg gtcaaccaaa tgactaacaa cgtcgtcatt aacgattctg ccaatgttac cttggccatt ggtgcctctc ctatcatggc gacccatcct cgtgacgtcc atgatctcag tcccgccatc ggagctctct tgatcaattt tggcaccatc acagacaaag caggcatgct tgtggctggc cgacaggcca acattaacag gaaacccatc atttttgacc ctgtggccat cggcgcaact ccataccggc aagaaacgtc tgtcgagctt cttgctcact ggcagccgac aattatcaag ggtaacgctg gtgaaatcgg attcatggcg aaatcgacag aagtcgccag tcgaggtgtt gattccgttg gttctgggtt ctctgatcca ggtgctgtcg tcaaagcact cgctcgaaag caggccgcaa ttatcgtcct gacaggcgaa catgactata tctcagatgg ttcaaccact ctcaaaatct ccaatggtca ccactaccta gaacgtatca ctggttctgg gtgccagctt ggatcggtta ttgcatcatt tgcagctacc gcaagactgg agcatctggc aaaacatggt gaatgggaga atgcgtcgca gctcgttcaa ggtgacatgt tggctgcggc tgtgaccggt gtgttggtgt ataccattgc cgcggaggtg gcggccgctc gtgaggatgt aaaagggcct gggacgttca gagccgcctt gattgatgaa ttgtacaacc tcaaccccga ggttttgcga cagcgagcca agattgagat cttgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat