CrneC.19399.a

NAD kinase

CENTER ID: CrneC.19399.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19399.a.B2.GE42589 NAD_kinase domain 222 653
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_04316
UniProt: J9VRG6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPDTRPSTPP PSSSVRGSRA PQPSPYLNVP TTARMRHPAA PSPLSKCDPT LENPRSISAM SSPDHTSLAA PSTSALAVPE ASPCFIHSHL DRHGSLQDWL KNKSANNRTH FVPDSPTSSK ARSNHGQHHH TNTNEPVLQS DVPFPSHNNQ GNISSPPSAS QPSSPRDNAS HVSLSRKGPP FNGYESDQSS VNEYGTRTPN LLNGLIGDGL LEGDDEGNGS LTKQLAETAQ GVREMSKELG RTKVRSRIQN VLIVTKARDN RLIKLTRELA LYLMQKRPTA SPDGSSSRHG NGSRDRGLVV YVDAQLQTSK RFDAAGLQRE YPHLFVPMAR QRSSSSASVN TLSHISAFSS TTSMGEAAKQ ATNGEGQLRY WTSELCSATP HLFDFVITLG GDGTVLFTSW LFQRIVPPVL PFALGSLGFL TNFDFYKYKE TIDKVVDEGI RVNLRMRFTC TVYRAVASGD IAVSKSKKRK AIKKPGGEIL MSRVDKGGWE SLEGPTPAVS PSEFEGEDKE IMCYSTRPVE QFEVLNDLVV DRGPSPYVSL LELFGDEHHL TTVQADGLTV STPTGSTAYS LSAGGSLAHP QIPAILITPI CPHTLSFRPM LLPDSMELRV CVPYNSRSTA WASFDGRGRV ELKQGDHIKV TASKYPFPTV CADKASTDWF SSISRTLRWN EREKQKSFVV VEEASDPPER SGDRKEDAKK DLFEEGRAKQ KFEKDAKASG DLVESEDEED DEEEIFDIDD KSGPSSPPVQ QQQQPTKNEM FPHQLHYLLS SHQNGLETPN RFVTPYSAPP ALSQRHLVEA IHKAEIHEKE KEKGQRDSIG ENPAPLRVDK SRHPSQLGRT VSQSTEEAEA ERVEREEKLR KEETPKHGQQ QRHHHHHHHH HHHHGHKPKR TPSNLAEKVA DCKAKAFAFF GQDDDSASDC SGSEE
NT Sequence
atgcccgata cgcgtccgtc tactcctcct ccttcgtctt ctgtccgagg ttcacgcgcc cctcaaccgt ctccatatct aaatgtacca acgacggctc gaatgcgcca tcccgccgca ccttctcctc tatctaagtg cgacccgacg ctcgaaaacc cgagatccat tagtgcgatg tcgtctcccg accacacgtc acttgcagca ccttccactt ctgccctggc tgtccctgag gcatcacctt gctttattca ttcacatctg gacagacatg gctcccttca ggattggctg aagaataaga gcgcgaataa taggacacat tttgtgcccg attcacccac ttcttcgaaa gctcgctcga accacggaca gcaccatcat acaaatacta atgaaccagt tctccagtcc gacgtcccat ttccatccca taataaccaa ggaaatatat cttcgccccc ttccgcgtcg caaccatcca gtcctagaga caatgcgtcc catgttagtc tttcccgtaa gggaccacca ttcaatggct acgaatctga tcaatcgagc gtgaatgaat acgggacaag gacgcctaac ttgctcaatg gtctaatagg tgatgggtta ctggaaggcg acgatgaagg gaatggaagt ctcacgaagc aattggcgga aactgcccag ggtgtcagag aaatgagcaa agaacttggc cgcaccaagg ttcgctcgcg aatccaaaac gtccttatag tgacgaaagc ccgtgacaat cggctaatta aactcactcg ggagctggcc ctgtatctca tgcaaaaacg cccgacggcg tcaccagacg ggagcagtag tcgtcacggt aatggatctc gtgaccgcgg cttagtcgtt tatgtcgatg cccaattgca gacatcgaag cgattcgatg ctgcagggct tcagcgcgaa tatcctcacc tttttgtgcc gatggcgaga caacgatctt cttcatccgc gtctgtcaac acattatcac atatctcagc attctcatca acaactagta tgggtgaggc cgccaaacag gcaactaacg gcgaaggaca attgagatac tggactagtg aactttgttc cgccacacca catttgtttg actttgtcat cactcttggt ggtgatggta ccgtgctttt tacatcttgg ctattccaac gaatcgtccc tccagtcctt ccctttgccc ttggatcttt gggtttcctc acaaactttg atttctataa atacaaggaa accattgaca aagtggtaga cgagggcatt cgcgtgaatc tgcgtatgcg attcacttgc actgtctatc gagctgtagc atcaggagat attgctgttt ccaaaagcaa aaaacggaag gcgatcaaga agcctggggg ggagattctg atgagtcgtg tagataaagg aggatgggaa agcttagagg gaccgactcc agctgtatcg ccctcggagt ttgagggtga agataaggaa atcatgtgtt attcaacccg ccctgtggag caatttgagg tgttaaatga cttagtggtt gaccgaggac ccagccccta cgtcagcttg ttagaactat tcggtgatga gcatcatctc acaactgtac aggccgatgg ccttactgtt tctaccccca cagggtctac ggcttactca ttatctgctg gaggctcgct tgctcatccg cagatccctg ctattctcat cacacccatc tgtccgcata ctctttcttt ccggcccatg ctgctaccag acagcatgga gttgagagtg tgtgttccat acaactctag aagcactgct tgggctagtt ttgatggtag agggcgggtg gagctcaaac agggcgatca cattaaggtt actgcatcaa aatatccctt tcccactgtc tgtgccgaca aagcctctac tgactggttt tcgtccatct cccgtacttt acgatggaac gaacgtgaga aacaaaaatc cttcgtggtt gtggaggaag ctagcgatcc tccggagaga tcaggggaca ggaaggaaga tgcgaaaaag gacctgtttg aagagggccg agctaaacaa aaatttgaga aagatgcaaa ggctagcggg gatctggtgg agagcgagga cgaagaggat gacgaagagg agatattcga tattgacgat aaatccggtc cgtcgtcgcc tcctgtgcaa caacagcagc agccgaccaa aaatgagatg ttcccacatc aactccatta tcttctctca tcccatcaaa acgggctaga aacgccgaac cgattcgtta cgccgtattc agctcctcct gcactatcgc agagacatct cgtggaggct atccacaaag ctgagattca cgaaaaggag aaagaaaagg gacagcgtga tagtataggg gaaaatccgg ctccattacg tgtagacaaa tctagacatc ccagccagtt ggggagaacg gtttcgcaga gcacagaaga agctgaggcc gaaagagtcg agcgggaaga gaagttgaga aaggaggaga cacccaaaca tggacaacaa caacgccatc atcatcacca ccaccaccat catcatcatc atggacataa gccgaaacgt acaccctcga acttggccga aaaggtggct gattgtaaag ccaaggcgtt tgcatttttt ggacaggatg atgactcagc ttccgattgc agtggttcag aggaataa
Details for CrneC.19399.a.B2.GE42589
HARVESTED ON: 8/5/2018
SEQUENCED ON: 8/8/2018
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMT KQLAETAQGV REMSKELGRT KVRSRIQNVL IVTKARDNRL IKLTRELALY LMQKRPTASP DGSSSRHGNG SRDRGLVVYV DAQLQTSKRF DAAGLQREYP HLFVPMARQR SSSSASVNTL SHISAFSSTT SMGEAAKQAT NGEGQLRYWT SELCSATPHL FDFVITLGGD GTVLFTSWLF QRIVPPVLPF ALGSLGFLTN FDFYKYKETI DKVVDEGIRV NLRMRFTCTV YRAVASGDIA VSKSKKRKAI KKPGGEILMS RVDKGGWESL EGPTPAVSPS EFEGEDKEIM CYSTRPVEQF EVLNDLVVDR GPSPYVSLLE LFGDEHHLTT VQADGLTVST PTGSTAYSLS AGGSLAHPQI PAILITPICP HTLSFRPMLL PDSMELRVCV PYNSRSTAWA SFDGRGRVEL KQGDHIKVTA SK
Validated NT Sequence
attttgatgc agtaacctta atgtgatcgc cctgtttgag ctccacccgc cctctaccat caaaactagc ccaagcagtg cttctagagt tgtatggaac acacactctc aactccatgc tgtctggtag cagcatgggc cggaaagaaa gagtatgcgg acagatgggt gtgatgagaa tagcagggat ctgcggatga gcaagcgagc ctccagcaga taatgagtaa gccgtagacc ctgtgggggt agaaacagta aggccatcgg cctgtacagt tgtgagatga tgctcatcac cgaatagttc taacaagctg acgtaggggc tgggtcctcg gtcaaccact aagtcattta acacctcaaa ttgctccaca gggcgggttg aataacacat gatttcctta tcttcaccct caaactccga gggcgataca gctggagtcg gtccctctaa gctttcccat cctcctttat ctacacgact catcagaatc tcccccccag gcttcttgat cgccttccgt tttttgcttt tggaaacagc aatatctcct gatgctacag ctcgatagac agtgcaagtg aatcgcatac gcagattcac gcgaatgccc tcgtctacca ctttgtcaat ggtttccttg tatttataga aatcaaagtt tgtgaggaaa cccaaagatc caagggcaaa gggaaggact ggagggacga ttcgttggaa tagccaagat gtaaaaagca cggtaccatc accaccaaga gtgatgacaa agtcaaacaa atgtggtgtg gcggaacaaa gttcactagt ccagtatctc aattgtcctt cgccgttagt tgcctgtttg gcggcctcac ccatactagt tgttgatgag aatgctgaga tatgtgataa tgtgttgaca gacgcggatg aagaagatcg ttgtctcgcc atcggcacaa aaaggtgagg atattcgcgc tgaagccctg cagcatcgaa tcgcttcgat gtctgcaatt gggcatcgac ataaacgact aagccgcggt cacgagatcc attaccgtga cgactactgc tcccgtctgg tgacgccgtc gggcgttttt gcatgagata cagggccagc tcccgagtga gtttaattag ccgattgtca cgggctttcg tcactataag gacgttttgg attcgcgagc gaaccttggt gcggccaagt tctttgctca tttctctgac accctgggca gtttccgcca attgcttcgt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHTK QLAETAQGVR EMSKELGRTK VRSRIQNVLI VTKARDNRLI KLTRELALYL MQKRPTASPD GSSSRHGNGS RDRGLVVYVD AQLQTSKRFD AAGLQREYPH LFVPMARQRS SSSASVNTLS HISAFSSTTS MGEAAKQATN GEGQLRYWTS ELCSATPHLF DFVITLGGDG TVLFTSWLFQ RIVPPVLPFA LGSLGFLTNF DFYKYKETID KVVDEGIRVN LRMRFTCTVY RAVASGDIAV SKSKKRKAIK KPGGEILMSR VDKGGWESLE GPTPAVSPSE FEGEDKEIMC YSTRPVEQFE VLNDLVVDRG PSPYVSLLEL FGDEHHLTTV QADGLTVSTP TGSTAYSLSA GGSLAHPQIP AILITPICPH TLSFRPMLLP DSMELRVCVP YNSRSTAWAS FDGRGRVELK QGDHIKVTAS KYPFPTVCAD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacgaagc aattggcgga aactgcccag ggtgtcagag aaatgagcaa agaacttggc cgcaccaagg ttcgctcgcg aatccaaaac gtccttatag tgacgaaagc ccgtgacaat cggctaatta aactcactcg ggagctggcc ctgtatctca tgcaaaaacg cccgacggcg tcaccagacg ggagcagtag tcgtcacggt aatggatctc gtgaccgcgg cttagtcgtt tatgtcgatg cccaattgca gacatcgaag cgattcgatg ctgcagggct tcagcgcgaa tatcctcacc tttttgtgcc gatggcgaga caacgatctt cttcatccgc gtctgtcaac acattatcac atatctcagc attctcatca acaactagta tgggtgaggc cgccaaacag gcaactaacg gcgaaggaca attgagatac tggactagtg aactttgttc cgccacacca catttgtttg actttgtcat cactcttggt ggtgatggta ccgtgctttt tacatcttgg ctattccaac gaatcgtccc tccagtcctt ccctttgccc ttggatcttt gggtttcctc acaaactttg atttctataa atacaaggaa accattgaca aagtggtaga cgagggcatt cgcgtgaatc tgcgtatgcg attcacttgc actgtctatc gagctgtagc atcaggagat attgctgttt ccaaaagcaa aaaacggaag gcgatcaaga agcctggggg ggagattctg atgagtcgtg tagataaagg aggatgggaa agcttagagg gaccgactcc agctgtatcg ccctcggagt ttgagggtga agataaggaa atcatgtgtt attcaacccg ccctgtggag caatttgagg tgttaaatga cttagtggtt gaccgaggac ccagccccta cgtcagcttg ttagaactat tcggtgatga gcatcatctc acaactgtac aggccgatgg ccttactgtt tctaccccca cagggtctac ggcttactca ttatctgctg gaggctcgct tgctcatccg cagatccctg ctattctcat cacacccatc tgtccgcata ctctttcttt ccggcccatg ctgctaccag acagcatgga gttgagagtg tgtgttccat acaactctag aagcactgct tgggctagtt ttgatggtag agggcgggtg gagctcaaac agggcgatca cattaaggtt actgcatcaa aatatccctt tcccactgtc tgtgccgact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat