SSGCID: Target Page

CrneC.19396.a

Sulfate adenylyltransferase (EC 2.7.7.4) (ATP-sulfurylase) (Sulfate adenylate transferase) (SAT)

CENTER ID:	CrneC.19396.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19396.a.B3.GE42588	APS_kinase domain		395	560

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_04215
UniProt:	Q8TG24

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MANAPHGGVL KDLLVRDAAL HDSLLEEARS LNDIFLTERQ LCDLELILNG GFSPLEGFMD EQDYTSVVET LRLAPFNGHK YGHVFPIPIT LDVSQEDINT LGLKQGARVA LRDPRDDAAL AILTVSDIYR PNKATEAEKV MGADDIAHPS VAYLRNNVKE FYVGGKVQAI QAPTHFDYVP LRYTPAELRA HFHKLAWRKV VAFQTRNPMH RAHRELTVRA ARQRRANVLI HPVVGLTKPG DVDHYTRVRA YQALMPSYPE GMAHLALLPL AMRMAGPREA VWHAVIRKNF GATHFIVGRD HAGPGKNSQG KDFYGPYDAQ ELVTQFKDEL QIEMVPFQAM TYLPGSDEYQ PVDEVPKGTP TADISGTELR KRLRTGASIP DWFSYTGVVK VLRESYPPRP QQGFTILLTG LHNSGKDTIA RALQVTLQQQ GSRSVSLLLG EELRSDLDPQ IGRAITPEQK HINLERIGFV AAELTKAGAA VIAAPTAPYE RSRQAFKKQV VGSGGGNYFL VHVATPLEWC EKVDRRGLYK AARAGEIKNL TGVDDVYEAP ENADLVCDLR IDTVPEIVHS IIMILESQNL V

NT Sequence

atggccaacg ctcctcacgg tggtgtcctc aaggacctcc ttgtccgcga tgctgctctt cacgattctc ttctcgaaga ggctcgtagc ttgaatgaca ttttccttac cgagcgtcaa ttgtgtgacc tcgagcttat cttgaacggt ggtttctctc ctcttgaagg tttcatggac gagcaggact acacttccgt cgttgagact ctccgacttg cccctttcaa cggtcataag tacggtcacg ttttccccat tcccattact ctcgatgtct ctcaggagga catcaacact cttggcctca agcagggtgc ccgagtcgct ctccgagacc cgcgtgacga tgctgccctt gctatcctta ctgtctctga catctaccga cctaacaagg ccaccgaggc tgaaaaggtc atgggtgctg acgacatcgc ccacccttcc gtcgcatacc tccgcaacaa cgttaaggag ttttacgtcg gcggtaaggt ccaggctatc caagctccta ctcactttga ctacgttccc cttcgataca ctcccgccga gctccgagct cacttccaca agctcgcctg gcgaaaggtt gtcgctttcc agacccgaaa ccctatgcac cgtgcccacc gagagctcac cgtccgagct gcccgtcaac gacgagcaaa cgtcctcatc caccccgttg tcggtcttac caagcccgga gatgttgatc actacactcg tgtccgagct taccaggctc tcatgccctc ttatcccgag ggtatggccc accttgctct cttgcccctc gctatgagga tggctggtcc cagggaggct gtctggcacg ccgttatccg aaagaacttt ggtgcgaccc acttcattgt cggtcgagac cacgccggtc ccggtaagaa ctctcagggt aaggacttct atggtcctta cgacgcccag gagctcgtta cccagttcaa ggatgagctt cagatcgaga tggttccctt ccaggccatg acctacctcc ccggttctga cgagtaccag cccgtcgatg aggtccccaa gggcacccct accgccgata tctctggtac cgagctccga aagcgcctcc gaactggcgc ctccatcccc gattggttct cttacacagg tgtcgtcaag gttctccgag aatcttatcc ccctcgaccc caacagggtt tcaccatcct cttgaccggt cttcacaact ctggtaagga cactattgcc cgagctcttc aagttactct ccaacaacag ggctctcgat ctgtctccct cctcctcggt gaggaactcc gatcagacct cgacccccag attggccgtg ctatcactcc cgagcaaaag cacatcaacc tcgaacgaat cggtttcgtc gcggccgagc ttacaaaggc cggtgccgcc gtcatcgctg ctcccaccgc tccttacgag aggtcccgac aggctttcaa gaagcaggtc gtcggctccg gtggcggtaa ctacttcttg gttcacgttg ctactccttt ggaatggtgt gagaaggtcg acaggagagg attgtacaag gctgccaggg ctggtgagat caagaacttg actggtgtcg atgatgtgta cgaggccccc gagaacgccg atttggtctg cgacttgagg attgacactg ttcccgagat cgtccactcc atcattatga tcctcgagag ccagaatctt gtttaa

Details for CrneC.19396.a.B3.GE42588

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	19kDa
OBSERVED MW:	19kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS YPPRPQQGFT ILLTGLHNSG KDTIARALQV TLQQQGSRSV SLLLGEELRS DLDPQIGRAI TPEQKHINLE RIGFVAAELT KAGAAVIAAP TAPYERSRQA FKKQVVGSGG GNYFLVHVAT PLEWCEKVDR RGLYKAARAG EIKNLTGVDD VYEAPENADL VCDLR

Validated NT Sequence

atcttactca cttacctcaa gtcgcagacc aaatcggcgt tctcgggggc ctcgtacaca tcatcgacac cagtcaagtt cttgatctca ccagccctgg cagccttgta caatcctctc ctgtcgacct tctcacacca ttccaaagga gtagcaacgt gaaccaagaa gtagttaccg ccaccggagc cgacgacctg cttcttgaaa gcctgtcggg acctctcgta aggagcggtg ggagcagcga tgacggcggc accggccttt gtaagctcgg ccgcgacgaa accgattcgt tcgaggttga tgtgcttttg ctcgggagtg atagcacggc caatctgggg gtcgaggtct gatcggagtt cctcaccgag gaggagggag acagatcgag agccctgttg ttggagagta acttgaagag ctcgggcaat agtgtcctta ccagagttgt gaagaccggt caagaggatg gtgaaaccct gttggggtcg agggggataa gacatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHSY PPRPQQGFTI LLTGLHNSGK DTIARALQVT LQQQGSRSVS LLLGEELRSD LDPQIGRAIT PEQKHINLER IGFVAAELTK AGAAVIAAPT APYERSRQAF KKQVVGSGGG NYFLVHVATP LEWCEKVDRR GLYKAARAGE IKNLTGVDDV YEAPENADLV CDLR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcttatc cccctcgacc ccaacagggt ttcaccatcc tcttgaccgg tcttcacaac tctggtaagg acactattgc ccgagctctt caagttactc tccaacaaca gggctctcga tctgtctccc tcctcctcgg tgaggaactc cgatcagacc tcgaccccca gattggccgt gctatcactc ccgagcaaaa gcacatcaac ctcgaacgaa tcggtttcgt cgcggccgag cttacaaagg ccggtgccgc cgtcatcgct gctcccaccg ctccttacga gaggtcccga caggctttca agaagcaggt cgtcggctcc ggtggcggta actacttctt ggttcacgtt gctactcctt tggaatggtg tgagaaggtc gacaggagag gattgtacaa ggctgccagg gctggtgaga tcaagaactt gactggtgtc gatgatgtgt acgaggcccc cgagaacgcc gatttggtct gcgacttgag gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat