SSGCID: Target Page

CrneC.19394.a

Pentafunctional AROM polypeptide

CENTER ID:	CrneC.19394.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
CrneC.19394.a.B4.GE42583	shikimate kinase domain	900	1048
CrneC.19394.a.B5.GE42584	DHQuinase/Shikimate_DH multi-domains	1085	1607
CrneC.19394.a.B6.GE42585	DHP/EPSP_synthase multi-domains	79	856

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_03701
UniProt:	J9VIG8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSSSADVLK ISILGNESIH VGFHLLPYIF KTVTTTLPSS TYVLITDTNL AAIYLNDLKA SFEEATAEAD NKARFLVYEV APGEGAKSRK VKGEIEDWML DNKCTRDTVI LAFGGGVIGD LTGFVAATFM RGVKFVQIPT TLLAMVDSSV GGKTAIDTPH GKNLIGAFWQ PSYIFADLAF LTTLPTREVS NGMAEVVKTA AIWKDDDFAL LESRSAEISL AASSRPTGAP TAGRFISDRS HAQSLLLQVV SGSIYVKAHI VTIDERETGL RNLVNFGHTI GHAIEAVLTP AMLHGECVSV GIVLEAEVAR QLGVLSQVAV GRLTRCLQAY GLPVSLSDRR IAALPASSQL SVDRLLDIMK IDKKNSGPAK KIVLLSRIGK TYEEKASVVA DNVIRKVLCE AVTVKAATPS KSPITMATPG SKSISNRALV LAALGKGTCR VRNLLHSDDT AVMMNALVEL KGAVFSWEDG GDTIVVEGGG GILSTPAKGK ELYLGNAGTA SRFLTTVCAM VSGSASSERS TIITGNARMK QRPIGPLVDA LTANGAKVKY LESTGCLPLD ISTDGFRGGH IQLAASVSSQ YVSSILLCAP YAAEQVTLEL TGGQVISQPY IDMTIAMMEQ FGATVERQKD EQGNLLDVYV IPKCTYVNPS EYSVESDASS ATYPLAIAAI TGTTCTISNI GSSSLQGDAR FAKEVLEPMG CIVEQTLTST KVTGPPVGTL RALGNVDMEP MTDAFLTASV LAAVAVKPCL PERKVEGLPE TASRIYGIAN QRVKECNRIQ AMRDQLAKFG VETDEFDDGI IIFGKPEASL FRGASIHCYD DHRVAMAFAV LSCIIDETII EEKRCVEKTW PNFWDDLQNK IGVAVEGVEL ETHNQASTSA KPVSPIDQSQ SDRPIFLIGM RGAGKTYVGR MAAEILSGQF TDADDVFAQE TRQSVSEFVA ANGWDEFRKK ETEILSKFVE EHRGNHVIAL GGGIVETEAA RETLKAHVAK GGHVVHVTRA LEDIEAYLDS IGNTAVRPNW GETFADVFKR REPWYQACSS HEFYNVLEAV GGQTHEEHTK AMRAECGRFF KFITGRESNR PRLSAGNPTS FLSLTFPDIT PALVHLDELT EGADAVEFRI DLLNTTGHAP TSPASPPISF VAKQLASLRL ATTLPIVFSV RSKDQGGMVP SDNAEAYGAL VRLGLRCACE YVDLEVCWPE QLLDSIVQLK RETHIIASWH DWTGDMAWDG EEMKAKHVLC EKYGDVAKIV GTAKSGLDNA KLAIFVGEVQ SHPGAKPLLA INMGAAGQLS RVLNPILTPV THDALPSRAA PGQLTAREIL QARALTGSLP AKKFVLFGSP IAHSVSPLLH NTGFATLGLP HNYGLHESEK VDQGVLEMIR SPEFGGASVT IPLKLDIIPH LDSVSEDAKI IGAVNTVIPR GGKLYGENTD WQAIRQAAAQ NLDDDVLSDG SSTALVIGAG GTCRAAIYAM HKLRFKTIYL FNRTPENAAK VKASFPESYN IAIVTSLSSL PEAPVVVVST VPGNSLTLDT FSQGIYLPSE VFSRPKGVAI DLAYKPHMTA LLQAAEKKEG WKALPGVEIL CLQGFKQFEE WTGKRAPEKK MRKAVLDKYF A

NT Sequence

atgtcttcgt cctctgccga tgtcctcaaa atatccatcc tggggaatga gtccatccac gttggattcc atctccttcc ctatatcttc aaaaccgtca caaccactct cccttcttcc acctacgtcc ttatcaccga cacgaatctt gccgcaatct atctcaatga cctcaaggcc tctttcgaag aggctacagc ggaggccgac aacaaggcaa ggttcctcgt ctatgaagtc gcccctggag agggcgcgaa gagccgaaaa gtcaagggcg aaatcgagga ctggatgctt gataacaagt gcaccagaga tactgtgatc cttgcattcg gtggcggtgt cattggcgat cttactggat ttgtcgctgc tactttcatg cgaggcgtca agtttgtcca aatcccgacg acccttcttg cgatggttga ttcttctgtt ggtggaaaga ccgctatcga taccccacat ggcaagaacc tcattggtgc attctggcag ccaagctaca tcttcgctga cctcgctttc ctaaccacac ttcctaccag ggaggtctcc aacggcatgg ccgaggtggt caagaccgct gctatctgga aagatgacga tttcgctctt ctcgagtcac gctctgccga gatttctcta gcagcttctt ctcgtcccac cggcgcgcct accgctggtc gttttatctc tgaccgttct cacgcgcagt ctctccttct tcaggtcgtt tctggatcaa tctatgttaa ggctcatatt gtgactattg atgagcgaga gactggtctt cggaaccttg tcaactttgg acataccatc ggacatgcca tcgaggctgt ccttactccc gctatgcttc acggcgagtg tgtctcagtc ggtattgttc ttgaggccga agtcgctagg caactaggtg ttctgagtca agttgctgtt ggacgtctta ccagatgtct tcaagcttat ggcttaccag tctccctctc tgacaggcga attgccgctc tccctgcctc gagccagctt tcggttgacc gtcttctcga cattatgaag attgacaaga agaactcggg tcccgccaaa aaaatcgttc tcctctctcg gattggcaaa acctacgagg agaaggcctc tgttgttgcc gacaacgtca tccgcaaagt cttgtgcgaa gcagttaccg taaaggctgc cactcccagc aaatccccaa ttaccatggc tacacctgga agtaagagta tttctaaccg tgctttggtg cttgctgccc ttggtaaggg cacatgtagg gtaaggaacc tcttgcactc tgacgatact gccgtcatga tgaacgctct cgtagagctt aagggtgctg tgttttcctg ggaagatggt ggcgatacca ttgtcgttga aggcggaggc ggcattctct ccactccagc aaagggcaag gagctttacc tcggtaatgc cggcactgcc agtcgatttc tcaccactgt ttgcgccatg gtctcaggct cagcttcaag cgagagatcg acgatcatta ctggcaatgc ccgtatgaag cagcgtccca tcgggccttt ggtcgacgct ctcaccgcca atggcgccaa ggtcaagtat cttgagtcta cgggatgcct ccctcttgac attagcacag acggattccg tggagggcac atccagcttg ctgcgtctgt gtcttctcaa tacgtttcct caatccttct ttgtgctcct tacgctgctg agcaggtgac ccttgagctt actggtgggc aggtcatttc ccagccctac atcgatatga ccatcgccat gatggaacaa tttggtgcca ctgtcgagcg ccaaaaggat gagcaaggta acttgcttga cgtctacgtg attcccaagt gtacatatgt caatccttcc gagtacagcg tcgaatccga tgcgtctagt gctacctacc cactcgccat cgcggccatc actggtacga cctgtactat atccaacatc ggctcttctt ctcttcaggg tgacgcgcgc tttgccaaag aggtcctcga acccatgggc tgtattgttg aacagacact taccagcacc aaggtcaccg gccctcctgt gggcactttg agggcactcg gtaacgttga catggagccc atgacagatg ctttcttgac tgcatcagtg ttggctgctg tggctgtcaa gccttgtctg cctgagagaa aggtcgaagg tttgcccgaa accgcttcaa gaatatacgg tatcgccaac caaagggtta aggagtgtaa cagaatccaa gccatgaggg atcaacttgc taaattcggt gttgagactg atgaatttga cgacggtatc atcatttttg gcaagcccga agcctcactc ttccgaggtg cttctattca ctgctatgac gatcatcgcg tggccatggc atttgcggtg ttgtcttgta tcattgacga aaccatcatc gaggaaaagc gctgcgttga gaagacttgg cccaatttct gggatgactt acaaaacaag attggtgttg ctgtggaagg cgtagagctt gaaacacaca accaggcctc cacttctgcc aaacccgttt ctcccattga ccaatcccag tccgatcgac ccatcttcct tatcggtatg cgtggtgctg gcaagaccta cgttggtcga atggccgccg aaatcctttc tggccaattc actgacgccg acgatgtttt cgctcaggag acccgccagt ctgtatccga attcgtcgcg gccaacggat gggacgaatt taggaaaaag gagaccgaga tcctaagcaa gttcgttgag gagcacaggg gtaatcacgt tatcgctctt ggcggtggta tagtggaaac tgaagcggcg agagagacct tgaaggcaca cgtggctaag ggtggccatg tcgttcacgt taccagggca ttggaggaca ttgaggccta tctcgactcc atcggaaaca ccgctgttag gcccaactgg ggtgagacct ttgcagacgt tttcaagagg agggagcctt ggtaccaggc ttgctccagc cacgaattct acaacgtcct tgaagcagtc ggcggccaga cgcatgagga acacaccaag gcgatgcgag cggaatgcgg aaggttcttc aagtttatca ctggtcgaga gtctaatcgc ccacgtctga gcgccggaaa ccctacatct ttcttgtcat tgacttttcc cgacatcact cctgctttgg tccacctcga cgagcttaca gagggggccg atgcagtgga gttccgaatc gatcttctta acacaaccgg tcacgcacct acaagtccag cttctcctcc tatctctttc gtggcgaagc agcttgccag ccttcgtctt gctaccacat tgcccatcgt tttctctgtg aggtccaagg accaaggagg tatggttcct tccgacaatg ccgaagctta tggggctttg gttcgtctcg gtttgcgttg cgcttgcgag tatgttgatc tcgaagtttg ctggccggag cagcttcttg atagtatcgt tcagctgaag cgtgaaacac acatcattgc gtcttggcac gattggacag gcgacatggc atgggatggt gaggagatga aggccaaaca cgttttgtgt gagaagtacg gtgatgtcgc gaagatcgtc ggtacggcta agtcaggatt ggataacgcc aagcttgcaa tctttgttgg tgaagttcaa agccatcctg gagccaagcc tttgttggct atcaacatgg gtgccgcggg acaactttcc agagttttga acccaatcct cactcctgtg acccatgatg ctcttccttc tcgagccgcc cctggtcaac tcactgcccg tgaaattctc caagctcgtg ccctcactgg ctcactcccg gccaagaagt tcgtgctttt cggtagtccc attgcccact ctgtctcacc gttattgcac aacaccggct tcgctacctt gggtttgccc cataactatg ggttgcacga gtctgagaaa gttgaccagg gcgtcttgga aatgattcga tccccagaat ttggtggtgc gagtgtcact ataccactca agctcgatat cattccccat cttgattcgg tttcggaaga tgccaagatc attggagctg tcaatacagt catccctcgg ggtggcaagc tctatggcga gaacactgac tggcaggcta ttcgtcaggc tgctgcgcag aaccttgatg atgatgttct cagcgatgga tcttccaccg ctctcgtgat cggtgctggt ggtacttgtc gtgcggcgat ttacgcaatg cacaagctcc gattcaagac catctacttg ttcaaccgga cacctgagaa cgctgcgaag gtcaaagctt ctttccccga gtcttacaac atcgcaatcg tcacatctct ctcttctttg cctgaggctc ctgttgtagt tgtctctact gtccctggta actctttgac actcgacacc ttttctcaag ggatctattt accatctgaa gtgttttccc gtcccaaggg tgttgccatt gacttggctt acaagcctca tatgactgct ttgttgcagg ctgcggaaaa gaaggaaggg tggaaggcct tacctggtgt agagatcttg tgtttgcagg gattcaaaca gtttgaagaa tggactggca agagggcgcc tgagaagaag atgaggaagg cagttttgga caagtacttc gcgtaa

Details for CrneC.19394.a.B4.GE42583

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	18kDa
OBSERVED MW:	18kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR GAGKTYVGRM AAEILSGQFT DADDVFAQET RQSVSEFVAA NGWDEFRKKE TEILSKFVEE HRGNHVIALG GGIVETEAAR ETLKAHVAKG GHVVHVTRAL EDIEAYLDSI GNTAVRPNWG ETFADVFKRR EPWYQACSSH EFYNVLE

Validated NT Sequence

atcttactca cttattcaag gacgttgtag aattcgtggc tggagcaagc ctggtaccaa ggctccctcc tcttgaaaac gtctgcaaag gtctcacccc agttgggcct aacagcggtg tttccgatgg agtcgagata ggcctcaatg tcctccaatg ccctggtaac gtgaacgaca tggccaccct tagccacgtg tgccttcaag gtctctctcg ccgcttcagt ttccactata ccaccgccaa gagcgataac gtgattaccc ctgtgctcct caacgaactt gcttaggatc tcggtctcct ttttcctaaa ttcgtcccat ccgttggccg cgacgaattc ggatacagac tggcgggtct cctgagcgaa aacatcgtcg gcgtcagtga attggccaga aaggatttcg gcggccattc gaccaacgta ggtcttgcca gcaccacgca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHMR GAGKTYVGRM AAEILSGQFT DADDVFAQET RQSVSEFVAA NGWDEFRKKE TEILSKFVEE HRGNHVIALG GGIVETEAAR ETLKAHVAKG GHVVHVTRAL EDIEAYLDSI GNTAVRPNWG ETFADVFKRR EPWYQACSSH EFYNVLE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg gtgctggcaa gacctacgtt ggtcgaatgg ccgccgaaat cctttctggc caattcactg acgccgacga tgttttcgct caggagaccc gccagtctgt atccgaattc gtcgcggcca acggatggga cgaatttagg aaaaaggaga ccgagatcct aagcaagttc gttgaggagc acaggggtaa tcacgttatc gctcttggcg gtggtatagt ggaaactgaa gcggcgagag agaccttgaa ggcacacgtg gctaagggtg gccatgtcgt tcacgttacc agggcattgg aggacattga ggcctatctc gactccatcg gaaacaccgc tgttaggccc aactggggtg agacctttgc agacgttttc aagaggaggg agccttggta ccaggcttgc tccagccacg aattctacaa cgtccttgaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for CrneC.19394.a.B5.GE42584

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	57kDa
OBSERVED MW:	57kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	89
PERCENT COVERAGE:	49

Validated AA Sequence

MAHHHHHHMA GNPTSFLSLT FPDITPALVH LDELTEGADA VEFRIDLLNT TGHAPTSPAS PPISFVAKQL ASLRLATTLP IVFSVRSKDQ GGMVPSDNAE AYGALVRLGL RCACEYVDLE VCWPEQLLDS IVQLKRETHI IASWHDWTGD MAWDGEEMKA KHVLCEKYGD VAKIVGTAKS GLDNAKLAIF VGEVQSHPGA KPLLAINMGA XXXLSRVLNP XLTPVTHDAL PSRAAPGQXX XXXXXXXSXX XXXTPXXXXX XXX

Validated NT Sequence

tcttctcagg cgccctcttg ccagtccatt cttcaaactg tttgaatccc tgcaaacaca agatctctac accaggtaag gccttccacc cttccttctt ttccgcagcc tgcaacaaag cagtcatatg aggcttgtaa gccaagtcaa tggcaacacc cttgggacgg gaaaacactt cagatggtaa atagatccct tgagaaaagg tgtcgagtgt caaagagtta ccagggacag tagagacaac tacaacagga gcctcaggca aagaagagag agatgtgacg attgcgatgt tgtaagactc ggggaaagaa gctttgacct tcgcagcgtt ctcaggtgtc cggttgaaca agtagatggt cttgaatcgg agcttgtgca ttgcgtaaat cgccgcacga caagtaccac cagcaccgat cacgagagcg gtggaagatc catcgctgag aacatcatca tcaaggttct gcgcagcagc ctgacgaata gcctgccagt cagtgttctc gccatagagc ttgccacccc gagggatgac tgtattgaca gctccaatga tcttggcatc ttccgaaacc gaatcaagat ggggaatgat atcgagcttg agtggtatag tgacactcgc accaccaaat tctggggatc gaatcatttc caagacgccc tggtcaactt tctcagactc gtgcaaccca tngttatggg gcaaacccaa ggtngcgaag ccggtgttgt gcaataacgg tgagacagag tgggcaatgg gactannnnn nnnnnnnnnn nnnnnnnncg ggagtnnnnn nnnnnnnnnn cgagnnnnnn nnannnnnnn nnnnnnnnng ttgaccaggg gcggctcgag aaggaagagc atcatgggtc acaggagtga gnnttgggtt caaaactctg gaaagttntn nnncggcacc catgttgata gccaacaaag gcttggctcc aggatggctt tgaacttcac caacaaagat tgcaagcttg gcgttatcca atcctgactt agccgtaccg acgatcttcg cgacatcacc gtacttctca cacaaaacgt gtttggcctt catctcctca ccatcccatg ccatgtcgcc tgtccaatcg tgccaagacg caatgatgtg tgtttcacgc ttcagctgaa cgatactatc aagaagctgc tccggccagc aaacttcgag atcaacatac tcgcaagcgc aacgcaaacc gagacgaacc aaagccccat aagcttcggc attgtcggaa ggaaccatac ctccttggtc cttggacctc acagagaaaa cgatgggcaa tgtggtagca agacgaaggc tggcaagctg cttcgccacg aaagagatag gaggagaagc tggacttgta ggtgcgtgac cggttgtgtt aagaagatcg attcggaact ccactgcatc ggccccctct gtaagctcgt cgaggtggac caaagcagga gtgatgtcgg gaaaagtcaa tgacaagaaa gatgtagggt ttccggccat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHAG NPTSFLSLTF PDITPALVHL DELTEGADAV EFRIDLLNTT GHAPTSPASP PISFVAKQLA SLRLATTLPI VFSVRSKDQG GMVPSDNAEA YGALVRLGLR CACEYVDLEV CWPEQLLDSI VQLKRETHII ASWHDWTGDM AWDGEEMKAK HVLCEKYGDV AKIVGTAKSG LDNAKLAIFV GEVQSHPGAK PLLAINMGAA GQLSRVLNPI LTPVTHDALP SRAAPGQLTA REILQARALT GSLPAKKFVL FGSPIAHSVS PLLHNTGFAT LGLPHNYGLH ESEKVDQGVL EMIRSPEFGG ASVTIPLKLD IIPHLDSVSE DAKIIGAVNT VIPRGGKLYG ENTDWQAIRQ AAAQNLDDDV LSDGSSTALV IGAGGTCRAA IYAMHKLRFK TIYLFNRTPE NAAKVKASFP ESYNIAIVTS LSSLPEAPVV VVSTVPGNSL TLDTFSQGIY LPSEVFSRPK GVAIDLAYKP HMTALLQAAE KKEGWKALPG VEILCLQGFK QFEEWTGKRA PEKKMRKAVL D

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccggaa accctacatc tttcttgtca ttgacttttc ccgacatcac tcctgctttg gtccacctcg acgagcttac agagggggcc gatgcagtgg agttccgaat cgatcttctt aacacaaccg gtcacgcacc tacaagtcca gcttctcctc ctatctcttt cgtggcgaag cagcttgcca gccttcgtct tgctaccaca ttgcccatcg ttttctctgt gaggtccaag gaccaaggag gtatggttcc ttccgacaat gccgaagctt atggggcttt ggttcgtctc ggtttgcgtt gcgcttgcga gtatgttgat ctcgaagttt gctggccgga gcagcttctt gatagtatcg ttcagctgaa gcgtgaaaca cacatcattg cgtcttggca cgattggaca ggcgacatgg catgggatgg tgaggagatg aaggccaaac acgttttgtg tgagaagtac ggtgatgtcg cgaagatcgt cggtacggct aagtcaggat tggataacgc caagcttgca atctttgttg gtgaagttca aagccatcct ggagccaagc ctttgttggc tatcaacatg ggtgccgcgg gacaactttc cagagttttg aacccaatcc tcactcctgt gacccatgat gctcttcctt ctcgagccgc ccctggtcaa ctcactgccc gtgaaattct ccaagctcgt gccctcactg gctcactccc ggccaagaag ttcgtgcttt tcggtagtcc cattgcccac tctgtctcac cgttattgca caacaccggc ttcgctacct tgggtttgcc ccataactat gggttgcacg agtctgagaa agttgaccag ggcgtcttgg aaatgattcg atccccagaa tttggtggtg cgagtgtcac tataccactc aagctcgata tcattcccca tcttgattcg gtttcggaag atgccaagat cattggagct gtcaatacag tcatccctcg gggtggcaag ctctatggcg agaacactga ctggcaggct attcgtcagg ctgctgcgca gaaccttgat gatgatgttc tcagcgatgg atcttccacc gctctcgtga tcggtgctgg tggtacttgt cgtgcggcga tttacgcaat gcacaagctc cgattcaaga ccatctactt gttcaaccgg acacctgaga acgctgcgaa ggtcaaagct tctttccccg agtcttacaa catcgcaatc gtcacatctc tctcttcttt gcctgaggct cctgttgtag ttgtctctac tgtccctggt aactctttga cactcgacac cttttctcaa gggatctatt taccatctga agtgttttcc cgtcccaagg gtgttgccat tgacttggct tacaagcctc atatgactgc tttgttgcag gctgcggaaa agaaggaagg gtggaaggcc ttacctggtg tagagatctt gtgtttgcag ggattcaaac agtttgaaga atggactggc aagagggcgc ctgagaagaa gatgaggaag gcagttttgg actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for CrneC.19394.a.B6.GE42585

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	84kDa
OBSERVED MW:	84kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHEV APGEGAKSRK VKGEIEDWML DNKCTRDTVI LAFGGGVIGD LTGFVAATFM RGVKFVQIPT TLLAMVDSSV GGKTAIDTPH GKNLIGAFWQ PSYIFADLAF LTTLPTREVS NGMAEVVKTA AIWKDDDFAL LESRSAEISL AASSRPTGAP TAGRFISDRS HAQSLLLQVV SGSIYVKAHI VTIDERETGL RNLVNFGHTI GHAIEAVLTP AMLHGECVSV GIVLEAEVAR QLGVLSQVAV GRLTRCLQAY GLPVSLSDRR IAALPASSQL SVDRLLDIMK IDKKNSGPAK KIVLLSRIGK TYEEKASVVA DNVIRKVLCE AVTVKAATPS KSPITMATPG SKSISNRALV LAALGKGTCR VRNLLHSDDT AVMMNALVEL KGAVFSWEDG GDTIVVEGGG GILSTPAKGK ELYLGNAGTA SRFLTTVCAM VSGSASSERS TIITGNARMK QRPIGPLVDA LTANGAKVKY LESTGCLPLD ISTDGFRGGH IQLAASVSSQ YVSSILLCAP YAAEQVTLEL TGGQVISQPY IDMTIAMMEQ FGATVERQKD EQGNLLDVYV IPKCTYVNPS EYSVESDASS ATYPLAIAAI TGTTCTISNI GSSSLQGDAR FAKEVLEPMG CIVEQTLTST KVTGPPVGTL RALGNVDMEP MTDAFLTASV LAAVAVKPCL PERKVEGLPE TASRIYGIAN QRVKECNRIQ AMRDQLAKFG VETDEFDDGI IIFGKPEASL FRGASIHCYD DHRVAMAFAV LSCIIDETII EEKRCVEKTW PNFWDD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaagtcg cccctggaga gggcgcgaag agccgaaaag tcaagggcga aatcgaggac tggatgcttg ataacaagtg caccagagat actgtgatcc ttgcattcgg tggcggtgtc attggcgatc ttactggatt tgtcgctgct actttcatgc gaggcgtcaa gtttgtccaa atcccgacga cccttcttgc gatggttgat tcttctgttg gtggaaagac cgctatcgat accccacatg gcaagaacct cattggtgca ttctggcagc caagctacat cttcgctgac ctcgctttcc taaccacact tcctaccagg gaggtctcca acggcatggc cgaggtggtc aagaccgctg ctatctggaa agatgacgat ttcgctcttc tcgagtcacg ctctgccgag atttctctag cagcttcttc tcgtcccacc ggcgcgccta ccgctggtcg ttttatctct gaccgttctc acgcgcagtc tctccttctt caggtcgttt ctggatcaat ctatgttaag gctcatattg tgactattga tgagcgagag actggtcttc ggaaccttgt caactttgga cataccatcg gacatgccat cgaggctgtc cttactcccg ctatgcttca cggcgagtgt gtctcagtcg gtattgttct tgaggccgaa gtcgctaggc aactaggtgt tctgagtcaa gttgctgttg gacgtcttac cagatgtctt caagcttatg gcttaccagt ctccctctct gacaggcgaa ttgccgctct ccctgcctcg agccagcttt cggttgaccg tcttctcgac attatgaaga ttgacaagaa gaactcgggt cccgccaaaa aaatcgttct cctctctcgg attggcaaaa cctacgagga gaaggcctct gttgttgccg acaacgtcat ccgcaaagtc ttgtgcgaag cagttaccgt aaaggctgcc actcccagca aatccccaat taccatggct acacctggaa gtaagagtat ttctaaccgt gctttggtgc ttgctgccct tggtaagggc acatgtaggg taaggaacct cttgcactct gacgatactg ccgtcatgat gaacgctctc gtagagctta agggtgctgt gttttcctgg gaagatggtg gcgataccat tgtcgttgaa ggcggaggcg gcattctctc cactccagca aagggcaagg agctttacct cggtaatgcc ggcactgcca gtcgatttct caccactgtt tgcgccatgg tctcaggctc agcttcaagc gagagatcga cgatcattac tggcaatgcc cgtatgaagc agcgtcccat cgggcctttg gtcgacgctc tcaccgccaa tggcgccaag gtcaagtatc ttgagtctac gggatgcctc cctcttgaca ttagcacaga cggattccgt ggagggcaca tccagcttgc tgcgtctgtg tcttctcaat acgtttcctc aatccttctt tgtgctcctt acgctgctga gcaggtgacc cttgagctta ctggtgggca ggtcatttcc cagccctaca tcgatatgac catcgccatg atggaacaat ttggtgccac tgtcgagcgc caaaaggatg agcaaggtaa cttgcttgac gtctacgtga ttcccaagtg tacatatgtc aatccttccg agtacagcgt cgaatccgat gcgtctagtg ctacctaccc actcgccatc gcggccatca ctggtacgac ctgtactata tccaacatcg gctcttcttc tcttcagggt gacgcgcgct ttgccaaaga ggtcctcgaa cccatgggct gtattgttga acagacactt accagcacca aggtcaccgg ccctcctgtg ggcactttga gggcactcgg taacgttgac atggagccca tgacagatgc tttcttgact gcatcagtgt tggctgctgt ggctgtcaag ccttgtctgc ctgagagaaa ggtcgaaggt ttgcccgaaa ccgcttcaag aatatacggt atcgccaacc aaagggttaa ggagtgtaac agaatccaag ccatgaggga tcaacttgct aaattcggtg ttgagactga tgaatttgac gacggtatca tcatttttgg caagcccgaa gcctcactct tccgaggtgc ttctattcac tgctatgacg atcatcgcgt ggccatggca tttgcggtgt tgtcttgtat cattgacgaa accatcatcg aggaaaagcg ctgcgttgag aagacttggc ccaatttctg ggatgactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at