CrneC.19392.a

Ribitol kinase

CENTER ID: CrneC.19392.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19392.a.B3.GE42577 FGGY_C 316 541
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_04631
UniProt: J9VY77

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDMEYFIGF DVGTGSGRAC LVDRNGNLIA EHAEPTLTHR SPTDARIFEQ STGNIWKSLS ICSKKILAES GVKPEQVKGL GFDATCSLAV VDKQGKPVSI SRAGQTEENE KDANLGLEGE WNVILWADHR AEEEAEKINS TGEGVLGFVG KTMSLEMEIP KTLWLSKHMA QEKFKQCMFF DLPDFLTYNA TSDLARSTCS LACKCSFVPI GATMTHDCDG GKEEVSTQGW SARFFEKIGL HDLVKEDFAS LGGIPGKNGL VLTAGQPVGK GLSKRAAEEL GLLEGTAVGS GVIDAYAGWI GTVAAAAGED QAQPTLEDAS TRLAAIAGTS TCHIAQSKDG ILVPGVWGPY RDAVFPNLWM NEGGQSSTGQ LIDFMMQSHP AYPKLLELSK SSGKSTFELL AERLETLQAE KAAKTLTHLT KDLHFYPDLH GNRSPLADPR MKGMITGLVL DDTLNDLAAK FNVTLEAIAL QTRHIVDEMN AKGHKIDSIY MSGSQAKNGP LMRLLATVLQ MPVIIPPQPS AAVVLGAAML GRYAYDLTTE RQGKPISSQQ EAEVAKEKDG WRLWDVMVQM TRPGKRIEPR NDKFGISEKR LLDVKYKIFR EAVEVQRRWR QMIADEVKEE E
NT Sequence
atgagcgaca tggagtactt catcggcttt gacgttggca ctggctctgg cagagcatgt ctcgtcgacc gcaacggcaa tcttatagct gagcacgctg aacctactct tacccaccgt tcccccaccg acgctcgtat cttcgagcaa tccacaggca acatctggaa atccctctcc atctgctcta agaagatcct cgctgagtct ggtgtcaagc ctgaacaagt taaaggtctc ggctttgacg ctacttgttc gcttgctgtc gtcgacaagc agggcaagcc tgtgagcatc tccagggcag gccagactga ggagaacgaa aaggatgcca acctcgggtt ggaaggggag tggaatgtta tcctttgggc cgaccaccga gctgaggagg aggctgaaaa gatcaactcg actggcgagg gcgtgcttgg tttcgtcggc aagactatga gtcttgagat ggagatcccc aagactctct ggttgagcaa gcacatggcc caggagaagt tcaagcagtg catgttcttt gaccttcctg actttcttac ctacaatgct acctctgacc ttgctcgttc cacttgctct ctcgcctgca agtgctcttt cgttcccatt ggagcaacta tgacccatga ctgtgatggc ggcaaggagg aggtcagcac ccagggttgg tctgctaggt ttttcgagaa gattggcctt cacgacttgg tcaaagaaga ttttgcttct ctcggtggta ttcccggcaa gaacggcctc gtcctaactg ctgggcaacc agtcggtaaa ggtctcagca agcgtgctgc cgaagagctc ggtcttttgg aagggacagc tgtcggatct ggtgttatcg atgcttatgc cggatggatc ggtaccgtcg ctgcagctgc tggggaagac caggctcagc ctactcttga ggatgctagt actaggcttg ctgctattgc tggtaccagt acatgccaca ttgcacaaag caaggatggt attttggtcc ctggtgtttg gggtccctac cgagatgccg tgttccctaa cctctggatg aacgaaggtg gccagtcttc taccggccag cttatcgact tcatgatgca aagccacccc gcctatccca agcttttgga gctttccaaa agttcgggca agagcacttt tgagttgctc gctgaacgat tagaaacttt gcaggcggag aaagctgcca agactttgac tcacttgaca aaggatttgc acttctaccc cgacttgcat ggtaaccgat ctcccctcgc cgatccccgt atgaagggta tgatcaccgg tctcgtcctt gacgacactc tcaacgacct cgcagccaaa ttcaatgtca cccttgaagc cattgccttg caaacccgcc acattgtcga cgagatgaac gccaaaggcc acaagattga ctctatctac atgagcggtt ctcaagctaa gaacggtcct ctcatgcgtc ttctcgcgac cgtgctccaa atgccggtca tcatcccccc tcaaccttca gctgccgtcg tactcggcgc agcgatgctt ggtcgatatg catatgacct cacgaccgaa cgacagggta aacccatttc gtcccagcaa gaagcggagg tagcgaagga aaaggatgga tggaggcttt gggatgtgat ggtgcagatg actcgtccgg ggaagaggat tgaacccagg aatgataagt ttgggattag tgagaagagg ttgttggatg tcaagtacaa gatctttaga gaggctgtag aggtgcagag aagatggcga cagatgattg ctgatgaggt caaggaagag gaataa
Details for CrneC.19392.a.B3.GE42577
HARVESTED ON: 8/5/2018
SEQUENCED ON: 8/8/2018
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML EDASTRLAAI AGTSTCHIAQ SKDGILVPGV WGPYRDAVFP NLWMNEGGQS STGQLIDFMM QSHPAYPKLL ELSKSSGKST FELLAERLET LQAEKAAKTL THLTKDLHFY PDLHGNRSPL ADPRMKGMIT GLVLDDTLND LAAKFNVTLE AIALQTRHIV DEMNAKGHKI DSIYMSGSQA KNGPLMRLLA TVLQMPVIIP PQPSAAVVLG AAMLGRYAYD LTTER
Validated NT Sequence
atcttactca cttatcgttc ggtcgtgagg tcatatgcat atcgaccaag catcgctgcg ccgagtacga cggcagctga aggttgaggg gggatgatga ccggcatttg gagcacggtc gcgagaagac gcatgagagg accgttctta gcttgagaac cgctcatgta gatagagtca atcttgtggc ctttggcgtt catctcgtcg acaatgtggc gggtttgcaa ggcaatggct tcaagggtga cattgaattt ggctgcgagg tcgttgagag tgtcgtcaag gacgagaccg gtgatcatac ccttcatacg gggatcggcg aggggagatc ggttaccatg caagtcgggg tagaagtgca aatcctttgt caagtgagtc aaagtcttgg cagctttctc cgcctgcaaa gtttctaatc gttcagcgag caactcaaaa gtgctcttgc ccgaactttt ggaaagctcc aaaagcttgg gataggcggg gtggctttgc atcatgaagt cgataagctg gccggtagaa gactggccac cttcgttcat ccagaggtta gggaacacgg catctcggta gggaccccaa acaccaggga ccaaaatacc atccttgctt tgtgcaatgt ggcatgtact ggtaccagca atagcagcaa gcctagtact agcatcctca agcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHLE DASTRLAAIA GTSTCHIAQS KDGILVPGVW GPYRDAVFPN LWMNEGGQSS TGQLIDFMMQ SHPAYPKLLE LSKSSGKSTF ELLAERLETL QAEKAAKTLT HLTKDLHFYP DLHGNRSPLA DPRMKGMITG LVLDDTLNDL AAKFNVTLEA IALQTRHIVD EMNAKGHKID SIYMSGSQAK NGPLMRLLAT VLQMPVIIPP QPSAAVVLGA AMLGRYAYDL TTER
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcttgagg atgctagtac taggcttgct gctattgctg gtaccagtac atgccacatt gcacaaagca aggatggtat tttggtccct ggtgtttggg gtccctaccg agatgccgtg ttccctaacc tctggatgaa cgaaggtggc cagtcttcta ccggccagct tatcgacttc atgatgcaaa gccaccccgc ctatcccaag cttttggagc tttccaaaag ttcgggcaag agcacttttg agttgctcgc tgaacgatta gaaactttgc aggcggagaa agctgccaag actttgactc acttgacaaa ggatttgcac ttctaccccg acttgcatgg taaccgatct cccctcgccg atccccgtat gaagggtatg atcaccggtc tcgtccttga cgacactctc aacgacctcg cagccaaatt caatgtcacc cttgaagcca ttgccttgca aacccgccac attgtcgacg agatgaacgc caaaggccac aagattgact ctatctacat gagcggttct caagctaaga acggtcctct catgcgtctt ctcgcgaccg tgctccaaat gccggtcatc atcccccctc aaccttcagc tgccgtcgta ctcggcgcag cgatgcttgg tcgatatgca tatgacctca cgaccgaacg atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat