CrneC.19390.a

Phosphatidylinositol 3-kinase (EC 2.7.1.137)

CENTER ID: CrneC.19390.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
CrneC.19390.a.B2.GE42506 PI3K_C2 40 189
CrneC.19390.a.B3.GE42507 PI3K_C2 extended C-term 40 223
CrneC.19390.a.B6.GE42510 PI3Ka 301 508
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_03821
UniProt: J9VG22

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQPGPSKDRD YSFARLCHLT PHLALKITSL QGSLPKHPFS RALHSPALAH QGLQQQTPSD LYVTCQLWAD GKQYSLPFRT AHKDFPRGYT WNSVVIFPIT YPSLLLSSQI AFTIWDVQGS GKAIPVGGTT MSLFNSKRTL KRGQQRLYVH RNVQADPSLN TTTPSEFPDE KEDEMGRLER LVKDFERGDI LKIDWLDRLT FRQIEKAHSA EAEKSDNLYL YVDLPKFDFP VVFSEQESLI TLPPQPVIHP PSQTSQPTSA LPPNLLSNDP HLWKTYDPEA WRENPVEIKH RKLLRSQRLG DEGRDLKPGP ADRDRLNEIF RLPPTASLSA VDKDLLWKFR FSLFRSPRSL TKFLKCITWS DPVEAKQAVE KLLPLWGQEV GMDDALELLG PNFTHKKVRA FAVKRLERAE DDELLLYLLQ LVQALKFEHK SSLDVQRGHR SHRKRERELA SQDEQSSGLS QFLINRSVAN PILGTSFHWY LMIECDNRSS VGKMYAQVAF NFMKKLSETP KGQAQRDILR RQGELVQILS TRAKDIRASK DSRSKKIEKL KAYLSDSKHG LASLPEPLPL PLNANIFVTS VVAEKSSIFK SNLLPLLIWF ETIDSTRPTD DDSPDAVVSI TPDYPIIFKN GDDLRQDQLV IQLFTLMDRL LRKENLDLRL SPYSVLATST TEGMIQFVPS KSVASIMAEH GSLQNYLRIE HADDGALGSY GIEASVMDTF VRSCAGYSVL TYVLGVGDRH LDNLMLAPDG HFFHVDFGYI LGRDPKPYPP PVKVCKEMVD AMGGPGSAHY GRFQSLCYTA FIGLRKNANL ILNLVALMVD AGIQDIQLEP DKAVWKVQEK FMLDLSEEDA IKQFEVLLND TSYLTAVFDR IHDWAQYLRD
NT Sequence
atgcagcccg ggccttccaa agacagggac tattcctttg cgaggctgtg ccatcttacc ccacacctcg ccctcaaaat cacctccctc caaggcagcc tgcccaagca ccctttctcc cgcgcactcc atagccccgc tctagcacac caaggtcttc aacagcaaac cccgtccgac ctctatgtca cctgccagct atgggccgac ggcaaacaat actcgctccc gtttagaacg gcacacaaag atttcccccg cggatacacg tggaactctg tcgtgatttt tcccataaca tatccatctt tactattgtc ttcacagata gcattcacta tatgggatgt acaggggtct ggaaaggcta tacctgtggg cggtaccact atgagtctgt tcaactctaa gcgcactcta aagcggggac agcaacggct atatgtccac aggaatgtcc aagcagatcc aagcttgaac accactacac caagcgagtt tcccgatgaa aaagaggatg agatgggaag gcttgaaaga ttagtcaagg atttcgaaag aggggatatc ctcaaaatag attggcttga tcgacttacc tttcgtcaga ttgagaaggc ccactcggct gaagctgaaa aatcggacaa tttgtatcta tatgtcgatc tacccaagtt tgacttccct gtagtcttct cggaacaaga atctctcata acccttccac cccaacctgt aattcacccg ccgtcacaaa cttctcagcc tacctcggcc ctaccgccca atctattatc aaatgatcca catctgtgga aaacatatga tcccgaggcc tggagggaaa atcctgtgga aatcaaacac agaaagctgc taagaagtca gcggctaggc gatgaaggca gagatctcaa accaggacca gctgatcgtg atcggttaaa cgaaatattc cgcttgccgc ctactgcatc actctccgca gtggataagg acctactatg gaagtttcgt ttctctctct ttcgctctcc ccgatctctt accaagttcc tcaagtgcat cacttggtcg gacccggtgg aagcaaaaca agcggtggag aagctgctac cgttgtgggg gcaagaagtt ggtatggatg atgccctgga gcttttgggg cctaatttca cacataagaa agttcgggca tttgcagtaa aacggttgga acgagctgaa gatgatgaac tattactgta tctgcttcaa ctagtgcagg ctctaaaatt tgagcacaaa tcatctttag acgttcagcg gggccatcgt agccatcgca aacgggaaag agaactggcg tctcaggacg aacagagtag cgggctatca caattcctga tcaatcgaag cgtagcaaat cccatattag ggacgagttt tcattggtat ttgatgatcg aatgtgataa cagatcatct gtagggaaaa tgtacgcaca agtggccttc aattttatga aaaagctatc agaaacacct aaaggccaag ctcagcgaga catccttcgg cgacaaggtg aactggttca aatactatcc acccgagcca aagatattcg tgcctcaaaa gattctagat ccaaaaaaat tgaaaaactc aaggcatacc tgtctgattc aaaacacggg cttgcttctc ttcctgaacc gcttccgctt cccctcaacg ccaacatttt cgtcacgtct gttgttgctg agaaatcatc catcttcaaa tctaacctct taccacttct catatggttt gagaccatag actcaaccag acccacagat gatgattccc ctgacgctgt tgtcagcatt acaccagatt atcccatcat tttcaaaaat ggagatgatc tgcgccaaga tcagcttgta atccagctgt ttactttgat ggatcgcctt ctacgcaaag aaaaccttga tctccgtttg agtccataca gtgtattggc aacttcaacc acagaaggca tgatacagtt tgtgcccagc aagagtgtag cttcgatcat ggcagagcat ggaagtttac aaaattattt gagaatagag cacgcggatg atggcgctct aggatcctat ggaatcgaag ccagtgtaat ggacacgttt gttagaagtt gcgctgggta ctctgtcctc acttatgtgt taggtgtcgg agatagacac ttagataatc tgatgctagc gccagatggt catttcttcc atgtggattt cgggtacatc ctcggtcgtg accccaagcc atacccaccc ccggtcaaag tctgtaaaga gatggtggac gcgatgggcg gcccaggatc tgcccattat ggaagattcc agagcctatg ctacaccgca tttatcggat tgaggaaaaa tgcaaacctg atattgaatc ttgtcgcgtt aatggtagat gctggcatcc aagatataca gctggagcca gataaagctg tatggaaggt gcaggagaaa ttcatgctgg atttgtcgga ggaagacgca atcaaacaat tcgaagtatt attgaatgat acttcatatc tgactgccgt gtttgatcgt attcatgatt gggcgcagta tcttcgggat tag
Details for CrneC.19390.a.B2.GE42506
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 18kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS RALHSPALAH QGLQQQTPSD LYVTCQLWAD GKQYSLPFRT AHKDFPRGYT WNSVVIFPIT YPSLLLSSQI AFTIWDVQGS GKAIPVGGTT MSLFNSKRTL KRGQQRLYVH RNVQADPSLN TTTPSEFPDE KEDEMGRLER LVKDFERGN
Validated NT Sequence
atggctcacc accaccacca ccatatgtcc cgcgcactcc atagccccgc tctagcacac caaggtcttc aacagcaaac cccgtccgac ctctatgtca cctgccagct atgggccgac ggcaaacaat actcgctccc gtttagaacg gcacacaaag atttcccccg cggatacacg tggaactctg tcgtgatttt tcccataaca tatccatctt tactattgtc ttcacagata gcattcacta tatgggatgt acaggggtct ggaaaggcta tacctgtggg cggtaccact atgagtctgt tcaactctaa gcgcactcta aagcggggac agcaacggct atatgtccac aggaatgtcc aagcagatcc aagcttgaac accactacac caagcgagtt tcccgatgaa aaagaggatg agatgggaag gcttgaaaga ttagtcaagg atttcgaaag aggaaattaa gtgagtaaga t
Expected Protein Sequence
MAHHHHHHSR ALHSPALAHQ GLQQQTPSDL YVTCQLWADG KQYSLPFRTA HKDFPRGYTW NSVVIFPITY PSLLLSSQIA FTIWDVQGSG KAIPVGGTTM SLFNSKRTLK RGQQRLYVHR NVQADPSLNT TTPSEFPDEK EDEMGRLERL VKDFERGD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcccgcg cactccatag ccccgctcta gcacaccaag gtcttcaaca gcaaaccccg tccgacctct atgtcacctg ccagctatgg gccgacggca aacaatactc gctcccgttt agaacggcac acaaagattt cccccgcgga tacacgtgga actctgtcgt gatttttccc ataacatatc catctttact attgtcttca cagatagcat tcactatatg ggatgtacag gggtctggaa aggctatacc tgtgggcggt accactatga gtctgttcaa ctctaagcgc actctaaagc ggggacagca acggctatat gtccacagga atgtccaagc agatccaagc ttgaacacca ctacaccaag cgagtttccc gatgaaaaag aggatgagat gggaaggctt gaaagattag tcaaggattt cgaaagaggg gattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for CrneC.19390.a.B3.GE42507
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 22kDa
OBSERVED MW: 22kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS RALHSPALAH QGLQQQTPSD LYVTCQLWAD GKQYSLPFRT AHKDFPRGYT WNSVVIFPIT YPSLLLSSQI AFTIWDVQGS GKAIPVGGTT MSLFNSKRTL KRGQQRLYVH RNVQADPSLN TTTPSEFPDE KEDEMGRLER LVKDFERGDI LKIDWLDRLT FRQIEKAHSA EAEKSDNLYL YVD
Validated NT Sequence
atggctcacc accaccacca ccatatgtcc cgcgcactcc atagccccgc tctagcacac caaggtcttc aacagcaaac cccgtccgac ctctatgtca cctgccagct atgggccgac ggcaaacaat actcgctccc gtttagaacg gcacacaaag atttcccccg cggatacacg tggaactctg tcgtgatttt tcccataaca tatccatctt tactattgtc ttcacagata gcattcacta tatgggatgt acaggggtct ggaaaggcta tacctgtggg cggtaccact atgagtctgt tcaactctaa gcgcactcta aagcggggac agcaacggct atatgtccac aggaatgtcc aagcagatcc aagcttgaac accactacac caagcgagtt tcccgatgaa aaagaggatg agatgggaag gcttgaaaga ttagtcaagg atttcgaaag aggggatatc ctcaaaatag attggcttga tcgacttacc tttcgtcaga ttgagaaggc ccactcggct gaagctgaaa aatcggacaa tttgtatcta tatgtcgatt aagtgagtaa gat
Expected Protein Sequence
MAHHHHHHSR ALHSPALAHQ GLQQQTPSDL YVTCQLWADG KQYSLPFRTA HKDFPRGYTW NSVVIFPITY PSLLLSSQIA FTIWDVQGSG KAIPVGGTTM SLFNSKRTLK RGQQRLYVHR NVQADPSLNT TTPSEFPDEK EDEMGRLERL VKDFERGDIL KIDWLDRLTF RQIEKAHSAE AEKSDNLYLY VD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcccgcg cactccatag ccccgctcta gcacaccaag gtcttcaaca gcaaaccccg tccgacctct atgtcacctg ccagctatgg gccgacggca aacaatactc gctcccgttt agaacggcac acaaagattt cccccgcgga tacacgtgga actctgtcgt gatttttccc ataacatatc catctttact attgtcttca cagatagcat tcactatatg ggatgtacag gggtctggaa aggctatacc tgtgggcggt accactatga gtctgttcaa ctctaagcgc actctaaagc ggggacagca acggctatat gtccacagga atgtccaagc agatccaagc ttgaacacca ctacaccaag cgagtttccc gatgaaaaag aggatgagat gggaaggctt gaaagattag tcaaggattt cgaaagaggg gatatcctca aaatagattg gcttgatcga cttacctttc gtcagattga gaaggcccac tcggctgaag ctgaaaaatc ggacaatttg tatctatatg tcgattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for CrneC.19390.a.B6.GE42510
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHDE GRDLKPGPAD RDRLNEIFRL PPTASLSAVD KDLLWKFRFS LFRSPRSLTK FLKCITWSDP VEAKQAVEKL LPLWGQEVGM DDALELLGPN FTHKKVRAFA VKRLERAEDD ELLLYLLQLV QALKFEHKSS LDVQRGHRSH RKRERELASQ DEQSSGLSQF LINRSVANPI LGTSFHWYLM IECDNRSSVG KMYAQVAFNF MKKLSE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgatgaag gcagagatct caaaccagga ccagctgatc gtgatcggtt aaacgaaata ttccgcttgc cgcctactgc atcactctcc gcagtggata aggacctact atggaagttt cgtttctctc tctttcgctc tccccgatct cttaccaagt tcctcaagtg catcacttgg tcggacccgg tggaagcaaa acaagcggtg gagaagctgc taccgttgtg ggggcaagaa gttggtatgg atgatgccct ggagcttttg gggcctaatt tcacacataa gaaagttcgg gcatttgcag taaaacggtt ggaacgagct gaagatgatg aactattact gtatctgctt caactagtgc aggctctaaa atttgagcac aaatcatctt tagacgttca gcggggccat cgtagccatc gcaaacggga aagagaactg gcgtctcagg acgaacagag tagcgggcta tcacaattcc tgatcaatcg aagcgtagca aatcccatat tagggacgag ttttcattgg tatttgatga tcgaatgtga taacagatca tctgtaggga aaatgtacgc acaagtggcc ttcaatttta tgaaaaagct atcagaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at