CrneC.19389.a

6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase

CENTER ID:	CrneC.19389.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19389.a.B4.GE42505	His_phos1 domain		233	432

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_07372
UniProt:	J9VH91

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAAPLYVTQS GRLWHAGLIL IVTVGLPARG KTHISRALER YLRWLGVKTR VYSIGDYRRK VLGGAKDVPH DYFQTKTPRS EATNALRRRI KAELEDQIMD FFTVQGGQVV IYDANNGSVA ERKSTLEKFG NKGVHVIYLE SLCDQEDIIT ANIRSVKLSS PDYAGWDAEK AVADYWERIR DQTEVYDTVT ADEGPFIKVM NVGERIEVNR IEGYLQTRCC FFLMNIHTRP RNIYFARSGQ SLIEHSYKAD SDLSPAGWEY AERLKAAVIA RRKAAREERK AKGEVLGEEN PLLIWTSARR RAYHTAWPFV HSGYKVVQKP IMSEINPGVW DGLSTQEAME LYPDEWTRFL ADPYAHRAPR AESYHDLSVR LESVIFELER CQDDLLIIGH ASVIRCLLAY LVGLPPNEVP AVEIARGDLV EITPASYGVI SRAWHFWSGE GRGDAYGENL YENFAEATSG KGSVLPDSGV NFAADALNME KEAEEEEGRE KEERGEKVME AAKAVGKIAE QAAANTAAGA GVANSSGDGA GAGGVGQGAR GPGKALKRWG SERGKGRGLP GLSELNETDE GEENKDKDDG GVVSEGEGEG RESGKTRSRA MSLLEI

NT Sequence

atggccgccc cgctctacgt cacgcagtcc ggccgtctgt ggcacgccgg cctcatcctc atcgtcaccg tcggcctccc agcccgcggc aagacacaca tctcccgcgc cctcgaacgc tacctccgct ggctcggcgt caagacccgc gtctactcca tcggcgacta caggcgcaaa gtcctcggcg gcgcaaagga cgtcccccac gactacttcc agacaaagac gcccagatca gaggccacca acgctctccg ccgccgcatc aaggcagaac tcgaagacca gatcatggac tttttcaccg tgcagggcgg ccaggtcgtc atctatgatg ccaacaatgg cagtgtcgca gagagaaaaa gcacgctgga aaagtttggc aacaagggcg tccatgtcat ttatcttgag agtctatgtg accaagaaga tatcatcacc gccaatatcc gcagcgtcaa gctctcgtct cccgactacg ccggatggga cgcagaaaag gcagtcgcag actactggga acgtatccga gaccagactg aagtgtacga caccgtcacg gccgatgaag ggccttttat caaggtgatg aatgtcggcg agaggatcga agtgaaccgt atcgaaggat accttcaaac gcgatgctgc ttcttcttga tgaatatcca cacccgccct cgaaacattt actttgcgcg ttctggacaa tccctcattg aacactcgta taaagccgat tctgacctct ccccggcagg ctgggaatat gctgaacgac tcaaagctgc ggtgatcgcg cggcgcaaag ctgcaagaga agagagaaag gcaaaaggcg aagtcctcgg agaggaaaac cctctgctca tatggacatc cgcgcgccgt cgtgcatacc acaccgcctg gccctttgtc cattccggct acaaggtcgt ccagaaaccc atcatgtcgg aaatcaaccc cggcgtctgg gacggcctct cgacacaaga agccatggaa ctctaccccg acgaatggac ccgtttcctc gccgacccgt atgcgcatcg agcaccccga gcggaaagtt atcatgattt gagtgtgcgg ctcgaaagcg ttattttcga gttggagagg tgtcaggatg atttgttgat tatcggtcat gcttccgtta tccgttgtct cctcgcgtac cttgtcggtc tgcccccgaa cgaagtcccc gcggtcgaaa tcgcgcgggg cgacctcgtc gaaatcacac ccgcctcata cggcgtcatc tcccgcgcat ggcacttttg gtccggtgaa ggccgggggg acgcgtatgg cgaaaacttg tatgaaaact ttgcagaagc gacctctggc aagggttcag tcctgcccga ttcgggggtc aattttgcgg cggatgcgct gaatatggag aaggaggcgg aagaggagga agggagggag aaggaggaga gaggggagaa ggtgatggaa gcggcaaagg cggtggggaa gattgcagag caggctgcgg cgaatacggc agctggtgcc ggtgtggcta acagtagtgg agatggcgca ggcgcaggcg gggtcggtca aggtgcaagg ggaccaggaa aggcgttgaa gagatggggt tcagagaggg ggaaagggag gggtttgccc gggctgagcg agttgaacga gacggatgaa ggggaggaaa acaaggacaa ggatgatggt ggggtagttt cggaaggcga gggtgagggg agagaaagtg gcaagacgag aagtagagct atgagcctct tggaaatcta g

Details for CrneC.19389.a.B4.GE42505

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/10/2018
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI YFARSGQSLI EHSYKADSDL SPAGWEYAER LKAAVIARRK AAREERKAKG EVLGEENPLL IWTSARRRAY HTAWPFVHSG YKVVQKPIMS EINPGVWDGL STQEAMELYP DEWTRFLADP YAHRAPRAES YHDLSVRLES VIFELERCQD DLLIIGHASV IRCLLAYLVG LPPNEVPAVE IARGDLVEIT PASYGVISR

Validated NT Sequence

atggctcacc accaccacca ccatatgatt tactttgcgc gttctggaca atccctcatt gaacactcgt ataaagccga ttctgacctc tccccggcag gctgggaata tgctgaacga ctcaaagctg cggtgatcgc gcggcgcaaa gctgcaagag aagagagaaa ggcaaaaggc gaagtcctcg gagaggaaaa ccctctgctc atatggacat ccgcgcgccg tcgtgcatac cacaccgcct ggccctttgt ccattccggc tacaaggtcg tccagaaacc catcatgtcg gaaatcaacc ccggcgtctg ggacggcctc tcgacacaag aagccatgga actctacccc gacgaatgga cccgtttcct cgccgacccg tatgcgcatc gagcaccccg agcggaaagt tatcatgatt tgagtgtgcg gctcgaaagc gttattttcg agttggagag gtgtcaggat gatttgttga ttatcggtca tgcttccgtt atccgttgtc tcctcgcgta ccttgtcggt ctgcccccga acgaagtccc cgcggtcgaa atcgcgcggg gcgacctcgt cgaaatcaca cccgcctcat acggcgtcat ctcccgctaa gtgagtaaga t

Expected Protein Sequence

MAHHHHHHIY FARSGQSLIE HSYKADSDLS PAGWEYAERL KAAVIARRKA AREERKAKGE VLGEENPLLI WTSARRRAYH TAWPFVHSGY KVVQKPIMSE INPGVWDGLS TQEAMELYPD EWTRFLADPY AHRAPRAESY HDLSVRLESV IFELERCQDD LLIIGHASVI RCLLAYLVGL PPNEVPAVEI ARGDLVEITP ASYGVISR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatttact ttgcgcgttc tggacaatcc ctcattgaac actcgtataa agccgattct gacctctccc cggcaggctg ggaatatgct gaacgactca aagctgcggt gatcgcgcgg cgcaaagctg caagagaaga gagaaaggca aaaggcgaag tcctcggaga ggaaaaccct ctgctcatat ggacatccgc gcgccgtcgt gcataccaca ccgcctggcc ctttgtccat tccggctaca aggtcgtcca gaaacccatc atgtcggaaa tcaaccccgg cgtctgggac ggcctctcga cacaagaagc catggaactc taccccgacg aatggacccg tttcctcgcc gacccgtatg cgcatcgagc accccgagcg gaaagttatc atgatttgag tgtgcggctc gaaagcgtta ttttcgagtt ggagaggtgt caggatgatt tgttgattat cggtcatgct tccgttatcc gttgtctcct cgcgtacctt gtcggtctgc ccccgaacga agtccccgcg gtcgaaatcg cgcggggcga cctcgtcgaa atcacacccg cctcatacgg cgtcatctcc cgctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat