CrneC.19388.a

Pyruvate dehydrogenase kinase

CENTER ID: CrneC.19388.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19388.a.B2.GE42502 BCDHK/PDK_N 26 275
CrneC.19388.a.B3.GE42503 His kinase/HATPase domain 322 446
CrneC.19388.a.B4.GE42504 PDK, multi-domain 23 456
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_00047
UniProt: J9VKF9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRFKISGAL WDSIHHYSSF PQTGVSLQQM IHFGHNPTPG TLLKASQFLS EELPIRLSHR VVELNALPDG LAKMPSINKV KEWYAQSFEE LVTFPKPRFK PELEGILNVP NSDGTKLAFP SSTPNPSLDP LMDEGPVGSG LLVERHNHGN GSGNGNGNGN GNGNGQRLRV PMERRYYSPP PATIVYPPEV HEYNERFTHL LENIKKRHDP TVTTVAQGVL EWKRKRKAGR IGAPIQEWLD RFHMSRIGIR FLIGQHVALN TLQPHPDYVG IICTRANVHD ICHEAIENAR YVCEEHYGLF RGPPIQLLCP KDLTFPYVPG HLSHICFELL KNSLRAVVER FGVENEEAFP PIKVVVVEGR EDITIKISDE GGGIPRSAIP MIWTYLYTTM SDEGLEATIE QSDFKAPMAG FGYGLPLARL YARFFGGDLR LISMDGYGTD VYISLNKLSS SCEPLQ
NT Sequence
atgtctcgat tcaagatttc aggtgccctc tgggacagca tccaccacta ttcatctttt ccacagactg gagtttccct ccaacaaatg attcatttcg gccataaccc cacacctggt acactcctca aagcttctca gtttctttct gaagagctgc ctatcaggtt atcacatagg gtagtagaac tcaacgcctt acctgatgga ctcgccaaga tgcctagtat caacaaggta aaggagtggt atgctcagag ctttgaggaa cttgtgacat ttccaaaacc tagattcaaa ccagaacttg aaggtatact caatgtccca aattcggacg gaacaaaact agctttcccc agctccactc ctaacccttc tctggaccca cttatggacg aaggccccgt cggttctggc cttctggtag agagacataa tcacggcaac gggagcggga acggtaatgg gaatggtaac ggaaacggca acgggcagag gttacgggta cccatggaac gtcggtacta ttcgccgcca cctgcaacga tcgtctaccc accggaagta catgagtaca acgaaaggtt tacccattta cttgagaata tcaagaaaag acacgatccg actgtcacta ccgttgccca aggtgtattg gagtggaaga ggaagcgaaa ggccggaagg ataggagcac caatacagga gtggttagat aggttccaca tgagccgaat tggtattcga ttcttgatag gacaacacgt tgcgctcaac actctacaac ctcatcctga ttatgtaggg ataatttgca cgcgagccaa cgtgcatgat atatgtcatg aagcgattga aaacgcccga tatgtctgtg aagagcacta cggtctcttc agagggccgc ctatccaact cctttgtcct aaagacctca cattcccata tgtacccggc cacctctccc acatctgctt tgaactcttg aaaaattcac ttcgtgcagt ggtagaacgt tttggagtgg agaacgagga agctttcccc ccgatcaagg tggttgtagt tgaaggtagg gaggatatca ccatcaagat ttcggatgaa ggtgggggga ttcccaggag tgcgattcca atgatctgga cgtaccttta tactacaatg agtgacgaag gtttagaagc gaccattgaa cagtcagact tcaaagcccc catggctggt ttcggctacg gccttcccct cgcaagactg tatgctagat tctttggtgg tgatctcaga ttaatctcta tggacgggta tgggacggac gtgtatatct ctctcaacaa gttgtcgtct agttgtgaac ctttgcaata a
Details for CrneC.19388.a.B2.GE42502
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 93
PERCENT COVERAGE: 107
Validated AA Sequence
MAHHHHHHMS LQQMIHFGHN PTPGTLLKAS QFLSEELPIR LSHRVVELNA LPDGLAKMPS INKVKEWYAQ SFEELVTFPK PRFKPELEGI LNVPNSDGTK LAFPSSTPNP SLDPLMDEGP VGSGLLVERH NHGNGSGNGN GNGNGNGNGQ RLRVPMERRY YSPPPATIVY PPEVHEYNER FTHLLENIKK RHDPTVTTVA QGVLEWKRKR KAGRIGAPIQ EWLDRFHMSR IGIRFLIGQR ESMMIARGKY GRSWKHQKDG ADLRAYRHCA QHSTTSS
Validated NT Sequence
atggctcacc accaccacca ccatatgtcc ctccaacaaa tgattcattt cggccataac cccacacctg gtacactcct caaagcttct cagtttcttt ctgaagagct gcctatcagg ttatcacata gggtagtaga actcaacgcc ttacctgatg gactcgccaa gatgcctagt atcaacaagg taaaggagtg gtatgctcag agctttgagg aacttgtgac atttccaaaa cctagattca aaccagaact tgaaggtata ctcaatgtcc caaattcgga cggaacaaaa ctagctttcc ccagctccac tcctaaccct tctctggacc cacttatgga cgaaggcccc gtcggttctg gccttctggt agagagacat aatcacggca acgggagcgg gaacggtaat gggaatggta acggaaacgg caacgggcag aggttacggg tacccatgga acgtcggtac tattcgccgc cacctgcaac gatcgtctac ccaccggaag tacatgagta caacgaaagg tttacccatt tacttgagaa tatcaagaaa agacacgatc cgactgtcac taccgttgcc caaggtgtat tggagtggaa gaggaagcga aaggccggaa ggataggagc accaatacag gagtggttag ataggttcca catgagccga attggtattc gattcttgat aggacaacgt gagtctatga tgattgctcg aggaaaatat ggtcgaagtt ggaagcacca aaaagatggc gctgacctta gagcctacag acattgcgct caacactcta caacctcatc ctgattntgt agggataatt tgcacgcgat aagtgagtaa gat
Expected Protein Sequence
MAHHHHHHSL QQMIHFGHNP TPGTLLKASQ FLSEELPIRL SHRVVELNAL PDGLAKMPSI NKVKEWYAQS FEELVTFPKP RFKPELEGIL NVPNSDGTKL AFPSSTPNPS LDPLMDEGPV GSGLLVERHN HGNGSGNGNG NGNGNGNGQR LRVPMERRYY SPPPATIVYP PEVHEYNERF THLLENIKKR HDPTVTTVAQ GVLEWKRKRK AGRIGAPIQE WLDRFHMSRI GIRFLIGQHV ALNTLQPHPD YVGIICTR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccctcc aacaaatgat tcatttcggc cataacccca cacctggtac actcctcaaa gcttctcagt ttctttctga agagctgcct atcaggttat cacatagggt agtagaactc aacgccttac ctgatggact cgccaagatg cctagtatca acaaggtaaa ggagtggtat gctcagagct ttgaggaact tgtgacattt ccaaaaccta gattcaaacc agaacttgaa ggtatactca atgtcccaaa ttcggacgga acaaaactag ctttccccag ctccactcct aacccttctc tggacccact tatggacgaa ggccccgtcg gttctggcct tctggtagag agacataatc acggcaacgg gagcgggaac ggtaatggga atggtaacgg aaacggcaac gggcagaggt tacgggtacc catggaacgt cggtactatt cgccgccacc tgcaacgatc gtctacccac cggaagtaca tgagtacaac gaaaggttta cccatttact tgagaatatc aagaaaagac acgatccgac tgtcactacc gttgcccaag gtgtattgga gtggaagagg aagcgaaagg ccggaaggat aggagcacca atacaggagt ggttagatag gttccacatg agccgaattg gtattcgatt cttgatagga caacacgttg cgctcaacac tctacaacct catcctgatt atgtagggat aatttgcacg cgatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for CrneC.19388.a.B3.GE42503
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 15kDa
OBSERVED MW: 15kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML SHICFELLKN SLRAVVERFG VENEEAFPPI KVVVVEGRED ITIKISDEGG GIPRSAIPMI WTYLYTTMSD EGLEATIEQS DFKAPMAGFG YGLPLARLYA RFFGGDLRLI SMDGYGTDVY ISLN
Validated NT Sequence
atggctcacc accaccacca ccatatgctc tcccacatct gctttgaact cttgaaaaat tcacttcgtg cagtggtaga acgttttgga gtggagaacg aggaagcttt ccccccgatc aaggtggttg tagttgaagg tagggaggat atcaccatca agatttcgga tgaaggtggg gggattccca ggagtgcgat tccaatgatc tggacgtacc tttatactac aatgagtgac gaaggtttag aagcgaccat tgaacagtca gacttcaaag cccccatggc tggtttcggc tacggccttc ccctcgcaag actgtatgct agattctttg gtggtgatct cagattaatc tctatggacg ggtatgggac ggacgtgtat atctctctca actaagtgag taagat
Expected Protein Sequence
MAHHHHHHLS HICFELLKNS LRAVVERFGV ENEEAFPPIK VVVVEGREDI TIKISDEGGG IPRSAIPMIW TYLYTTMSDE GLEATIEQSD FKAPMAGFGY GLPLARLYAR FFGGDLRLIS MDGYGTDVYI SLN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctctccc acatctgctt tgaactcttg aaaaattcac ttcgtgcagt ggtagaacgt tttggagtgg agaacgagga agctttcccc ccgatcaagg tggttgtagt tgaaggtagg gaggatatca ccatcaagat ttcggatgaa ggtgggggga ttcccaggag tgcgattcca atgatctgga cgtaccttta tactacaatg agtgacgaag gtttagaagc gaccattgaa cagtcagact tcaaagcccc catggctggt ttcggctacg gccttcccct cgcaagactg tatgctagat tctttggtgg tgatctcaga ttaatctcta tggacgggta tgggacggac gtgtatatct ctctcaactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for CrneC.19388.a.B4.GE42504
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 49kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMT GVSLQQMIHF GHNPTPGTLL KASQFLSEEL PIRLSHRVVE LNALPDGLAK MPSINKVKEW YAQSFEELVT FPKPRFKPEL EGILNVPNSD GTKLAFPSST PNPSLDPLMD EGPVGSGLLV ERHNHGNGSG NGNGNGNGNG NGQRLRVPME RRYYSPPPAT IVYPPEVHEY NERFTHLLEN IKKRHDPTVT TVAQGVLEWK RKRKAGRIGA PIQEWLDRFH MSRIGIRFLI GQHVALNTLQ PHPDYVGIIC TRANVHDICH EAIENARYVC EEHYGLFRGP PIQLLCPKDL TFPYVPGHLS HICFELLKNS LRAVVERFGV ENEEAFPPIK VVVVEGREDI TIKISDEGGG IPRSAIPMIW TYLYTTMSDE GLEATIEQSD FKAPMAGFGY GLPLARLYAR FFGGDLRLIS MDGYGTDVYI SLNKL
Validated NT Sequence
atggctcacc accaccacca ccatatgact ggagtttccc tccaacaaat gattcatttc ggccataacc ccacacctgg tacactcctc aaagcttctc agtttctttc tgaagagctg cctatcaggt tatcacatag ggtagtagaa ctcaacgcct tacctgatgg actcgccaag atgcctagta tcaacaaggt aaaggagtgg tatgctcaga gctttgagga acttgtgaca tttccaaaac ctagattcaa accagaactt gaaggtatac tcaatgtccc aaattcggac ggaacaaaac tagctttccc cagctccact cctaaccctt ctctggaccc acttatggac gaaggccccg tcggttctgg ccttctggta gagagacata atcacggcaa cgggagcggg aacggtaatg ggaatggtaa cggaaacggc aacgggcaga ggttacgggt acccatggaa cgtcggtact attcgccgcc acctgcaacg atcgtctacc caccggaagt acatgagtac aacgaaaggt ttacccattt acttgagaat atcaagaaaa gacacgatcc gactgtcact accgttgccc aaggtgtatt ggagtggaag aggaagcgaa aggccggaag gataggagca ccaatacagg agtggttaga taggttccac atgagccgaa ttggtattcg attcttgata ggacaacacg ttgcgctcaa cactctacaa cctcatcctg attatgtagg gataatttgc acgcgagcca acgtgcatga tatatgtcat gaagcgattg aaaacgcccg atatgtctgt gaagagcact acggtctctt cagagggccg cctatccaac tcctttgtcc taaagacctc acattcccat atgtacccgg ccacctctcc cacatctgct ttgaactctt gaaaaattca cttcgtgcag tggtagaacg ttttggagtg gagaacgagg aagctttccc cccgatcaag gtggttgtag ttgaaggtag ggaggatatc accatcaaga tttcggatga aggtgggggg attcccagga gtgcgattcc aatgatctgg acgtaccttt atactacaat gagtgacgaa ggtttagaag cgaccattga acagtcagac ttcaaagccc ccatggctgg tttcggctac ggccttcccc tcgcaagact gtatgctaga ttctttggtg gtgatctcag attaatctct atggacgggt atgggacgga cgtgtatatc tctctcaaca agttgt
Expected Protein Sequence
MAHHHHHHTG VSLQQMIHFG HNPTPGTLLK ASQFLSEELP IRLSHRVVEL NALPDGLAKM PSINKVKEWY AQSFEELVTF PKPRFKPELE GILNVPNSDG TKLAFPSSTP NPSLDPLMDE GPVGSGLLVE RHNHGNGSGN GNGNGNGNGN GQRLRVPMER RYYSPPPATI VYPPEVHEYN ERFTHLLENI KKRHDPTVTT VAQGVLEWKR KRKAGRIGAP IQEWLDRFHM SRIGIRFLIG QHVALNTLQP HPDYVGIICT RANVHDICHE AIENARYVCE EHYGLFRGPP IQLLCPKDLT FPYVPGHLSH ICFELLKNSL RAVVERFGVE NEEAFPPIKV VVVEGREDIT IKISDEGGGI PRSAIPMIWT YLYTTMSDEG LEATIEQSDF KAPMAGFGYG LPLARLYARF FGGDLRLISM DGYGTDVYIS LNKLSSSCEP LQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catactggag tttccctcca acaaatgatt catttcggcc ataaccccac acctggtaca ctcctcaaag cttctcagtt tctttctgaa gagctgccta tcaggttatc acatagggta gtagaactca acgccttacc tgatggactc gccaagatgc ctagtatcaa caaggtaaag gagtggtatg ctcagagctt tgaggaactt gtgacatttc caaaacctag attcaaacca gaacttgaag gtatactcaa tgtcccaaat tcggacggaa caaaactagc tttccccagc tccactccta acccttctct ggacccactt atggacgaag gccccgtcgg ttctggcctt ctggtagaga gacataatca cggcaacggg agcgggaacg gtaatgggaa tggtaacgga aacggcaacg ggcagaggtt acgggtaccc atggaacgtc ggtactattc gccgccacct gcaacgatcg tctacccacc ggaagtacat gagtacaacg aaaggtttac ccatttactt gagaatatca agaaaagaca cgatccgact gtcactaccg ttgcccaagg tgtattggag tggaagagga agcgaaaggc cggaaggata ggagcaccaa tacaggagtg gttagatagg ttccacatga gccgaattgg tattcgattc ttgataggac aacacgttgc gctcaacact ctacaacctc atcctgatta tgtagggata atttgcacgc gagccaacgt gcatgatata tgtcatgaag cgattgaaaa cgcccgatat gtctgtgaag agcactacgg tctcttcaga gggccgccta tccaactcct ttgtcctaaa gacctcacat tcccatatgt acccggccac ctctcccaca tctgctttga actcttgaaa aattcacttc gtgcagtggt agaacgtttt ggagtggaga acgaggaagc tttccccccg atcaaggtgg ttgtagttga aggtagggag gatatcacca tcaagatttc ggatgaaggt ggggggattc ccaggagtgc gattccaatg atctggacgt acctttatac tacaatgagt gacgaaggtt tagaagcgac cattgaacag tcagacttca aagcccccat ggctggtttc ggctacggcc ttcccctcgc aagactgtat gctagattct ttggtggtga tctcagatta atctctatgg acgggtatgg gacggacgtg tatatctctc tcaacaagtt gtcgtctagt tgtgaacctt tgcaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat