CrneC.19384.a

Dihydroxyacetone kinase

CENTER ID: CrneC.19384.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
CrneC.19384.a.B1.GE41076 full length 1 600
CrneC.19384.a.B2.GE41182 DhaK domain 1 354
CrneC.19384.a.B3.GE41183 DhaL domain 388 600
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_00826
UniProt: J9VF57

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDRHIFPDH KTLVFRSIRG LVASHPYLSL IPSQKVVYRA DHDPSKVSLI CGGGSGHEPG TVGFVGAGLL TASVAGDVFA SPSARQVMEA IKRVHSDKGT ILIITNYTGD NLHFGLAKLM AQSAGLKNVE LVVVGDDVSV PKSRGKYVGR RCLAGITLVC KILGAASEVD VEFQDLVTLG RSLSANTASV AMALDHCHVP GRSGEADWHL PEGKVEIGLG LHNETGVFSI AQPPPDVLIN KLLDLLLKQD DPERSFVKFK DGDELVLLVN NMGGMSVLEM GSVVDEVLIQ LESRGIIPTR ILNGPFMGSM NMPGISLSLL NLTNVAEECS FVDTSKLIGF LDAPHNSVAW PATSQVYPLP EKLANRKRED KFVDAEEEKK EEITGGPQLF GDKQFILKAM KQAAEDVLAS EPKLTKWDTI VGDGDCGETC ALGAQATLKA LEQGLGSDGE LVHFFRVLTE VIDGSMGGTL GAIFSIFLAG LTTALIDAAS SSNGTPKLTP AFFGQVTSSA LETLKARTAA RVGHRTVMDA LIPFGETLAE SGDLKKAVEA CRKGGESTVD LQAKLGRATY VGQLEGEMPP DPGAMAFVTV AEGILKAYSS
NT Sequence
atgacagacc gacacatctt cccagaccac aagactctgg tctttcgatc catcagaggt ctcgttgcct ctcaccctta tctttccttg atcccctccc agaaggtcgt ctaccgagca gaccacgacc cttccaaggt ttcccttatt tgcggtggtg gttccggcca tgagcctggt actgtcggat ttgttggagc tggtttgctc acagcgagtg tcgctggcga tgtgtttgcc agtcctagtg cgaggcaggt catggaggct atcaagcgcg tccacagcga caagggtacc attcttatta ttacaaacta tactggcgac aaccttcatt tcgggctagc caaactcatg gctcaatcag ctggtttgaa aaacgtcgag ctcgtggttg tgggcgatga cgtctcagtg ccgaaatccc gaggaaagta tgttggccga aggtgccttg cgggcataac tcttgtctgc aagattctcg gtgccgcctc cgaagttgat gtggaattcc aagacctcgt gacgctcggt cgctcccttt cggccaacac tgcctccgtc gccatggcct tggaccactg ccacgtccca ggcaggtcgg gggaagccga ctggcacttg cccgaaggta aagtcgagat tggtctcggt ttgcacaacg aaactggtgt tttcagcatt gctcagcctc ctcctgatgt tctcatcaat aagcttttgg atcttttgct caagcaagac gaccccgaga ggtcttttgt caagttcaag gacggtgatg aacttgtttt gctcgtgaac aacatgggcg gtatgagtgt cctcgagatg ggctctgtcg ttgatgaagt ccttattcag ctcgaatccc gaggtatcat ccccacacga atcctgaatg gccccttcat gggctccatg aacatgcccg gtatctctct ctccctcctc aacctcacca acgtcgccga agaatgttcc tttgtagaca cctcaaagct catcgggttc cttgatgctc cacacaattc tgtcgcttgg cctgccacga gccaggttta ccccttgcct gaaaagctgg caaacaggaa gagggaggat aagtttgtgg atgcggaaga ggagaagaag gaggagatta ctggcggacc tcagttgttc ggtgacaagc agtttattct caaggctatg aagcaagcag ctgaggatgt gctcgctagc gaacccaagc ttaccaagtg ggataccatt gtcggtgacg gtgactgtgg agaaacatgt gcccttggtg cccaagcgac ccttaaagct cttgaacaag gccttggctc tgacggcgaa ctggtccact ttttccgagt cttgactgaa gtcatcgacg gctcaatggg cgggaccctc ggcgccatct tctccatctt cctcgccggt ctcaccaccg ccctcatcga cgccgcttcc tcttctaacg gtacccccaa actcacccct gccttcttcg gccaagtcac ttcctctgcc ctcgaaactc tcaaagcccg tactgccgct cgagtcggtc accgaactgt catggacgcg cttattccct ttggagagac ccttgctgag agtggagatt tgaagaaggc cgtggaggcg tgtaggaaag gtggggagag cacggtggac ttgcaagcca agttgggtag agcgacgtat gtgggtcagt tggagggcga gatgcctccc gaccctggag cgatggcgtt tgtgactgtg gcggaaggta ttctcaaggc ttatagttct tag
Details for CrneC.19384.a.B1.GE41076
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 65kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMT DRHIFPDHKT LVFRSIRGLV ASHPYLSLIP SQKVVYRADH DPSKVSLICG GGSGHEPGTV GFVGAGLLTA SVAGDVFASP SARQVMEAIK RVHSDKGTIL IITNYTGDNL HFGLAKLMAQ SAGLKNVELV VVGDDVSVPK SRGKYVGRRC LAGITLVCKI LGAASEVDVE FQDLVTLGRS LSANTASVAM ALDHCHVPGR SGEADWHLPE GKVEIGLGLH NETGVFSIAQ PPPDVLINKL LDLLLKQDDP ERSFVKFKDG DELVLLVNNM GGMSVLEMGS VVDEVLIQLE SRGIIPTRIL NGPFMGSMNM PGISLSLLNL TNVAEECSFV DTSKLIGFLD APHNSVAWPA TSQVYPLPEK LANRKREDKF VDAEEEKKEE ITGGPQLFGD KQFILKAMKQ AAEDVLASEP KLTKWDTIVG DGDCGETCAL GAQATLKALE QGLGSDGELV HFFRVLTEVI DGSMGGTLGA IFSIFLAGLT TALIDAASSS NGTPKLTPAF FGQVTSSALE TLKARTAARV GHRTVMDALI PFGETLAESG DLKKAVEACR KGGESTVDLQ AKLGRATYVG QLEGEMPPDP GAMAFVTVAE GILKAYSS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacag accgacacat cttcccagac cacaagactc tggtctttcg atccatcaga ggtctcgttg cctctcaccc ttatctttcc ttgatcccct cccagaaggt cgtctaccga gcagaccacg acccttccaa ggtttccctt atttgcggtg gtggttccgg ccatgagcct ggtactgtcg gatttgttgg agctggtttg ctcacagcga gtgtcgctgg cgatgtgttt gccagtccta gtgcgaggca ggtcatggag gctatcaagc gcgtccacag cgacaagggt accattctta ttattacaaa ctatactggc gacaaccttc atttcgggct agccaaactc atggctcaat cagctggttt gaaaaacgtc gagctcgtgg ttgtgggcga tgacgtctca gtgccgaaat cccgaggaaa gtatgttggc cgaaggtgcc ttgcgggcat aactcttgtc tgcaagattc tcggtgccgc ctccgaagtt gatgtggaat tccaagacct cgtgacgctc ggtcgctccc tttcggccaa cactgcctcc gtcgccatgg ccttggacca ctgccacgtc ccaggcaggt cgggggaagc cgactggcac ttgcccgaag gtaaagtcga gattggtctc ggtttgcaca acgaaactgg tgttttcagc attgctcagc ctcctcctga tgttctcatc aataagcttt tggatctttt gctcaagcaa gacgaccccg agaggtcttt tgtcaagttc aaggacggtg atgaacttgt tttgctcgtg aacaacatgg gcggtatgag tgtcctcgag atgggctctg tcgttgatga agtccttatt cagctcgaat cccgaggtat catccccaca cgaatcctga atggcccctt catgggctcc atgaacatgc ccggtatctc tctctccctc ctcaacctca ccaacgtcgc cgaagaatgt tcctttgtag acacctcaaa gctcatcggg ttccttgatg ctccacacaa ttctgtcgct tggcctgcca cgagccaggt ttaccccttg cctgaaaagc tggcaaacag gaagagggag gataagtttg tggatgcgga agaggagaag aaggaggaga ttactggcgg acctcagttg ttcggtgaca agcagtttat tctcaaggct atgaagcaag cagctgagga tgtgctcgct agcgaaccca agcttaccaa gtgggatacc attgtcggtg acggtgactg tggagaaaca tgtgcccttg gtgcccaagc gacccttaaa gctcttgaac aaggccttgg ctctgacggc gaactggtcc actttttccg agtcttgact gaagtcatcg acggctcaat gggcgggacc ctcggcgcca tcttctccat cttcctcgcc ggtctcacca ccgccctcat cgacgccgct tcctcttcta acggtacccc caaactcacc cctgccttct tcggccaagt cacttcctct gccctcgaaa ctctcaaagc ccgtactgcc gctcgagtcg gtcaccgaac tgtcatggac gcgcttattc cctttggaga gacccttgct gagagtggag atttgaagaa ggccgtggag gcgtgtagga aaggtgggga gagcacggtg gacttgcaag ccaagttggg tagagcgacg tatgtgggtc agttggaggg cgagatgcct cccgaccctg gagcgatggc gtttgtgact gtggcggaag gtattctcaa ggcttatagt tcttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for CrneC.19384.a.B2.GE41182
HARVESTED ON: 3/1/2017
SEQUENCED ON: 3/2/2017
EXPECTED MW: 39kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMT DRHIFPDHKT LVFRSIRGLV ASHPYLSLIP SQKVVYRADH DPSKVSLICG GGSGHEPGTV GFVGAGLLTA SVAGDVFASP SARQVMEAIK RVHSDKGTIL IITNYTGDNL HFGLAKLMAQ SAGLKNVELV VVGDDVSVPK SRGKYVGRRC LAGITLVCKI LGAASEVDVE FQDLVTLGRS LSANTASVAM ALDHCHVPGR SGEADWHLPE GKVEIGLGLH NETGVFSIAQ PPPDVLINKL LDLLLKQDDP ERSFVKFKDG DELVLLVNNM GGMSVLEMGS VVDEVLIQLE SRGIIPTRIL NGPFMGSMNM PGISLSLLNL TNVAEECSFV DTSKLIGFLD APHNSVAWPA TS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacag accgacacat cttcccagac cacaagactc tggtctttcg atccatcaga ggtctcgttg cctctcaccc ttatctttcc ttgatcccct cccagaaggt cgtctaccga gcagaccacg acccttccaa ggtttccctt atttgcggtg gtggttccgg ccatgagcct ggtactgtcg gatttgttgg agctggtttg ctcacagcga gtgtcgctgg cgatgtgttt gccagtccta gtgcgaggca ggtcatggag gctatcaagc gcgtccacag cgacaagggt accattctta ttattacaaa ctatactggc gacaaccttc atttcgggct agccaaactc atggctcaat cagctggttt gaaaaacgtc gagctcgtgg ttgtgggcga tgacgtctca gtgccgaaat cccgaggaaa gtatgttggc cgaaggtgcc ttgcgggcat aactcttgtc tgcaagattc tcggtgccgc ctccgaagtt gatgtggaat tccaagacct cgtgacgctc ggtcgctccc tttcggccaa cactgcctcc gtcgccatgg ccttggacca ctgccacgtc ccaggcaggt cgggggaagc cgactggcac ttgcccgaag gtaaagtcga gattggtctc ggtttgcaca acgaaactgg tgttttcagc attgctcagc ctcctcctga tgttctcatc aataagcttt tggatctttt gctcaagcaa gacgaccccg agaggtcttt tgtcaagttc aaggacggtg atgaacttgt tttgctcgtg aacaacatgg gcggtatgag tgtcctcgag atgggctctg tcgttgatga agtccttatt cagctcgaat cccgaggtat catccccaca cgaatcctga atggcccctt catgggctcc atgaacatgc ccggtatctc tctctccctc ctcaacctca ccaacgtcgc cgaagaatgt tcctttgtag acacctcaaa gctcatcggg ttccttgatg ctccacacaa ttctgtcgct tggcctgcca cgagctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for CrneC.19384.a.B3.GE41183
HARVESTED ON: 3/1/2017
SEQUENCED ON: 3/2/2017
EXPECTED MW: 23kDa
OBSERVED MW: 23kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHQL FGDKQFILKA MKQAAEDVLA SEPKLTKWDT IVGDGDCGET CALGAQATLK ALEQGLGSDG ELVHFFRVLT EVIDGSMGGT LGAIFSIFLA GLTTALIDAA SSSNGTPKLT PAFFGQVTSS ALETLKARTA ARVGHRTVMD ALIPFGETLA ESGDLKKAVE ACRKGGESTV DLQAKLGRAT YVGQLEGEMP PDPGAMAFVT VAEGILKAYS S
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcagttgt tcggtgacaa gcagtttatt ctcaaggcta tgaagcaagc agctgaggat gtgctcgcta gcgaacccaa gcttaccaag tgggatacca ttgtcggtga cggtgactgt ggagaaacat gtgcccttgg tgcccaagcg acccttaaag ctcttgaaca aggccttggc tctgacggcg aactggtcca ctttttccga gtcttgactg aagtcatcga cggctcaatg ggcgggaccc tcggcgccat cttctccatc ttcctcgccg gtctcaccac cgccctcatc gacgccgctt cctcttctaa cggtaccccc aaactcaccc ctgccttctt cggccaagtc acttcctctg ccctcgaaac tctcaaagcc cgtactgccg ctcgagtcgg tcaccgaact gtcatggacg cgcttattcc ctttggagag acccttgctg agagtggaga tttgaagaag gccgtggagg cgtgtaggaa aggtggggag agcacggtgg acttgcaagc caagttgggt agagcgacgt atgtgggtca gttggagggc gagatgcctc ccgaccctgg agcgatggcg tttgtgactg tggcggaagg tattctcaag gcttatagtt cttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat