SSGCID: Target Page

CrneC.19380.a

Atypical/ABC1/ABC1-A protein kinase

CENTER ID:	CrneC.19380.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19380.a.B2.GE41075	kinase ADCK3 domain		222	619

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19380.a.B2.PW38238	kinase ADCK3 domain		222	619

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_02459
UniProt:	J9VUJ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHTTMLSAAL RVLARSAAIQ RGEAGTAAAT ATTAAPPPLS RAAALDRLIN AAPHLPPPPA PGTHPADKGH GIPLPLSLKA GHRGPVRYVQ PESRPTPNAG PSTSARPPEP ARPAKPVRPA NTMEPAKTVD ADKPPQPVPP IVQELDAGPG IKNHPVEPLI TLPEDEETPV ILRASRVPAS RLGRLFHYGS LAASLSWGAA SESIRRSTAG NGGEKSGGSV FMSDANIRRL VSTLGRMRGA ALKLGQFMSI QDNHMLPPEI EQVLHQVQAH ANYMPDWQMD KVLRDELGAD WQANLFTQFD RTPIASASIG QVHRAILKDG RQVAVKIQFP GVASSIESDL SNLSLLLRTS ALLPKGLYLQ NTIAVMRREL EDECDYIREA AAGKKFAHLL AGDTFFVVPQ VIDEATTGKV LTTEWMDGKP LSRVKNLSQE TRDLIGTNIL RLCLRELFQF RFMQTDPNWA NFLFSPSSPS PSPSSSPQIQ LIDFGASREY TKEFMDGWYR LLKSALEGDR ERMRVESLNL GYLTGEENDE MVQAHLDSMA LVASPFSHHG PYPFAKQTIT ESIRALIPIM LKHRLTPPPQ ETYSLNRKLS GAFLMCAKMR ANVDCKKLWE EEVGGYKEG

NT Sequence

atgcacacca ccatgctctc cgcagcactc agggtgctcg cacgctcggc agcgatccag cggggggagg cggggacggc agcggcgaca gcgacgacag cagcaccccc ccccctctcc cgcgcagcag ccctcgacag actcataaac gcagcgccgc acctcccccc cccgcccgca cccggcaccc atccagcaga caagggacac ggtattcccc tgcccctcag cctgaaagca ggccatcgcg gtcctgtcag gtatgtccag cctgaatcca ggcccactcc caatgctggc ccatccacct ctgctcgacc accggaacca gcgcgaccag ccaagccagt gcgaccagcg aacactatgg aaccagcgaa aactgtagac gccgataaac cgcctcagcc cgtcccgccc atcgtccaag agctcgacgc tggacctggc atcaaaaatc accctgttga acctctcatc acactcccag aagacgaaga gaccccggtg atcttgcgtg cgtccagagt acctgcttcc cgactcggtc gtcttttcca ctatggctca cttgccgcat cactctcatg gggcgccgca tcagaatcta tccggcggtc taccgcagga aacggcggcg aaaagagcgg tgggagtgtg tttatgagcg atgcgaatat caggcggctc gtctctaccc tcggccgcat gaggggtgct gcattaaagt tgggccaatt catgtctatc caagacaacc acatgctccc gcccgaaatc gagcaggtcc tgcaccaagt ccaagcccac gccaactaca tgcccgactg gcagatggac aaggtcctcc gcgacgaact cggtgccgac tggcaagcca acctgtttac ccaatttgac cgtacaccga tcgcgtccgc ctcgatcggt caagtgcatc gcgccatcct caaagatggg aggcaggtcg ccgtcaagat ccagttcccc ggcgtggcgt cctcgatcga atccgacttg tccaacctct ccctcctcct ccgcacatcg gcgctcttgc caaagggact gtatttgcaa aacacgatag cagtcatgcg gagagagttg gaagatgagt gtgattatat tagggaagca gcagccggca agaaattcgc acacctcttg gcgggcgata cgttttttgt agtgccccaa gtaatcgacg aagcgacgac cggtaaagtg ttgacgacag agtggatgga tgggaaaccg ttgagccggg tcaagaatct ctctcaagaa actcgtgatt tgattggcac caacatcctc cgtctctgtc ttcgcgaact ctttcaattc cgattcatgc aaaccgatcc caactgggca aactttttat tctccccttc ctccccttcc ccctctccat catcatcacc ccagatccag ctgatcgatt ttggcgcaag tagagaatac actaaagaat tcatggatgg gtggtatagg ttgttgaaaa gcgcgttgga aggggatagg gagaggatgc gcgtggagag tttgaatttg gggtatttga ccggggagga aaacgacgaa atggtccaag cgcatctcga ctccatggcc ctcgtcgcct cccccttctc ccaccacggc ccatacccat tcgccaaaca gacaatcacc gagtccatcc gcgccctcat ccccatcatg ctcaaacacc gtctcacccc gccaccgcag gaaacgtatt cgctcaatag gaaattgagt ggagcgttct tgatgtgtgc aaagatgcgg gcgaatgtgg attgtaagaa actttgggag gaggaagttg ggggttataa agagggttag

Details for CrneC.19380.a.B2.GE41075

HARVESTED ON:	2/8/2017
SEQUENCED ON:	2/13/2017
EXPECTED MW:	46kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMS DANIRRLVST LGRMRGAALK LGQFMSIQDN HMLPPEIEQV LHQVQAHANY MPDWQMDKVL RDELGADWQA NLFTQFDRTP IASASIGQVH RAILKDGRQV AVKIQFPGVA SSIESDLSNL SLLLRTSALL PKGLYLQNTI AVMRRELEDE CDYIREAAAG KKFAHLLAGD TFFVVPQVID EATTGKVLTT EWMDGKPLSR VKNLSQETRD LIGTNILRLC LRELFQFRFM QTDPNWANFL FSPSSPSPSP SSSPQIQLID FGASREYTKE FMDGWYRLLK SALEGDRERM RVESLNLGYL TGEENDEMVQ AHLDSMALVA SPFSHHGPYP FAKQTITESI RALIPIMLKH RLTPPPQETY SLNRKLSGAF LMCAKMRANV DCKKLWEE

Validated NT Sequence

cctcctccca aagtttctta caatccacat tcgcccgcat ctttgcacac atcaagaacg ctccactcaa tttcctattg agcgaatacg tttcctgcgg tggcggggtg agacggtgtt tgagcatgat ggggatgagg gcgcggatgg actcggtgat tgtctgtttg gcgaatgggt atgggccgtg gtgggagaag ggggaggcga cgagggccat ggagtcgaga tgcgcttgga ccatttcgtc gttttcctcc ccggtcaaat accccaaatt caaactctcc acgcgcatcc tctccctatc cccttccaac gcgcttttca acaacctata ccacccatcc atgaattctt tagtgtattc tctacttgcg ccaaaatcga tcagctggat ctggggtgat gatgatggag agggggaagg ggaggaaggg gagaataaaa agtttgccca gttgggatcg gtttgcatga atcggaattg aaagagttcg cgaagacaga gacggaggat gttggtgcca atcaaatcac gagtttcttg agagagattc ttgacccggc tcaacggttt cccatccatc cactctgtcg tcaacacttt accggtcgtc gcttcgtcga ttacttgggg cactacaaaa aacgtatcgc ccgccaagag gtgtgcgaat ttcttgccgg ctgctgcttc cctaatataa tcacactcat cttccaactc tctccgcatg actgctatcg tgttttgcaa atacagtccc tttggcaaga gcgccgatgt gcggaggagg agggagaggt tggacaagtc ggattcgatc gaggacgcca cgccggggaa ctggatcttg acggcgacct gcctcccatc tttgaggatg gcgcgatgca cttgaccgat cgaggcggac gcgatcggtg tacggtcaaa ttgggtaaac aggttggctt gccagtcggc accgagttcg tcgcggagga ccttgtccat ctgccagtcg ggcatgtagt tggcgtgggc ttggacttgg tgcaggacct gctcgatttc gggcgggagc atgtggttgt cttggataga catgaattgg cccaacttta atgcagcacc cctcatgcgg ccgagggtag agacgagccg cctgatattc gcatcgctca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg atgcgaatat caggcggctc gtctctaccc tcggccgcat gaggggtgct gcattaaagt tgggccaatt catgtctatc caagacaacc acatgctccc gcccgaaatc gagcaggtcc tgcaccaagt ccaagcccac gccaactaca tgcccgactg gcagatggac aaggtcctcc gcgacgaact cggtgccgac tggcaagcca acctgtttac ccaatttgac cgtacaccga tcgcgtccgc ctcgatcggt caagtgcatc gcgccatcct caaagatggg aggcaggtcg ccgtcaagat ccagttcccc ggcgtggcgt cctcgatcga atccgacttg tccaacctct ccctcctcct ccgcacatcg gcgctcttgc caaagggact gtatttgcaa aacacgatag cagtcatgcg gagagagttg gaagatgagt gtgattatat tagggaagca gcagccggca agaaattcgc acacctcttg gcgggcgata cgttttttgt agtgccccaa gtaatcgacg aagcgacgac cggtaaagtg ttgacgacag agtggatgga tgggaaaccg ttgagccggg tcaagaatct ctctcaagaa actcgtgatt tgattggcac caacatcctc cgtctctgtc ttcgcgaact ctttcaattc cgattcatgc aaaccgatcc caactgggca aactttttat tctccccttc ctccccttcc ccctctccat catcatcacc ccagatccag ctgatcgatt ttggcgcaag tagagaatac actaaagaat tcatggatgg gtggtatagg ttgttgaaaa gcgcgttgga aggggatagg gagaggatgc gcgtggagag tttgaatttg gggtatttga ccggggagga aaacgacgaa atggtccaag cgcatctcga ctccatggcc ctcgtcgcct cccccttctc ccaccacggc ccatacccat tcgccaaaca gacaatcacc gagtccatcc gcgccctcat ccccatcatg ctcaaacacc gtctcacccc gccaccgcag gaaacgtatt cgctcaatag gaaattgagt ggagcgttct tgatgtgtgc aaagatgcgg gcgaatgtgg attgtaagaa actttgggag gaggaagttg ggggttataa agagggttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for CrneC.19380.a.B2.PW38238

PURIFICATION DATe:	4/4/2017
CONCENTRATION:	33.5mg/ml
OBSERVED MW:	48kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	19
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG ATGCGAATAT CAGGCGGCTC GTCTCTACCC TCGGCCGCAT GAGGGGTGCT GCATTAAAGT TGGGCCAATT CATGTCTATC CAAGACAACC ACATGCTCCC GCCCGAAATC GAGCAGGTCC TGCACCAAGT CCAAGCCCAC GCCAACTACA TGCCCGACTG GCAGATGGAC AAGGTCCTCC GCGACGAACT CGGTGCCGAC TGGCAAGCCA ACCTGTTTAC CCAATTTGAC CGTACACCGA TCGCGTCCGC CTCGATCGGT CAAGTGCATC GCGCCATCCT CAAAGATGGG AGGCAGGTCG CCGTCAAGAT CCAGTTCCCC GGCGTGGCGT CCTCGATCGA ATCCGACTTG TCCAACCTCT CCCTCCTCCT CCGCACATCG GCGCTCTTGC CAAAGGGACT GTATTTGCAA AACACGATAG CAGTCATGCG GAGAGAGTTG GAAGATGAGT GTGATTATAT TAGGGAAGCA GCAGCCGGCA AGAAATTCGC ACACCTCTTG GCGGGCGATA CGTTTTTTGT AGTGCCCCAA GTAATCGACG AAGCGACGAC CGGTAAAGTG TTGACGACAG AGTGGATGGA TGGGAAACCG TTGAGCCGGG TCAAGAATCT CTCTCAAGAA ACTCGTGATT TGATTGGCAC CAACATCCTC CGTCTCTGTC TTCGCGAACT CTTTCAATTC CGATTCATGC AAACCGATCC CAACTGGGCA AACTTTTTAT TCTCCCCTTC CTCCCCTTCC CCCTCTCCAT CATCATCACC CCAGATCCAG CTGATCGATT TTGGCGCAAG TAGAGAATAC ACTAAAGAAT TCATGGATGG GTGGTATAGG TTGTTGAAAA GCGCGTTGGA AGGGGATAGG GAGAGGATGC GCGTGGAGAG TTTGAATTTG GGGTATTTGA CCGGGGAGGA AAACGACGAA ATGGTCCAAG CGCATCTCGA CTCCATGGCC CTCGTCGCCT CCCCCTTCTC CCACCACGGC CCATACCCAT TCGCCAAACA GACAATCACC GAGTCCATCC GCGCCCTCAT CCCCATCATG CTCAAACACC GTCTCACCCC GCCACCGCAG GAAACGTATT CGCTCAATAG GAAATTGAGT GGAGCGTTCT TGATGTGTGC AAAGATGCGG GCGAATGTGG ATTGTAAGAA ACTTTGGGAG GAGGAAGTTG GGGGTTATAA AGAGGGTTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT