CrneC.19379.a

PAB-dependent poly(A)-specific ribonuclease subunit PAN3 (PAB1P-dependent poly(A)-nuclease) (PAN deadenylation complex subunit 3)

CENTER ID: CrneC.19379.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19379.a.B2.GE41074 kinase-like domain 243 674
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_04821
UniProt: J9VXS7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLPPPKSAAV QIVRPPSPSS EKAKEKEKKH SPEKRETPQR ICRNVMIYGY CKYQDQGCIY YHPPAGADPS TPQESSPATH APTPLAGTPA REKPTLSIEH LAAPVFVPKG LDSSSPRVAT PSAPTPSAPT PPVWPSLPST GLLPRQDVHV SARPSHAQLS ATASPMAYDD PSHIALSAAH AHAQAQALTH SMLDPHAHAP PVDQSMYLPP RQPLDYNLYA APLPSIGGNP LYPAHPHAFF VNDDLRRSIQ AKQEAVYVGA NGASAPGLPQ ELGVYHSLVP LPLPAPTAQR LPTQSQPSKV YGLPSPVYRA TSEVDGNTYC LRRVEGFKLV NQLAFASMDT WRRMRHPNIV GLKEAFTTKT FGDNSLIMVY DYHPLSTTLY DEYLSPNPPE PSPASLLAGN QPPKRRSSPP ERILWSYVTQ IANALKAIHS SGLAVRNLDA SKILLTGKNR IRLNGCGVWD VLAFDNKTPV QAFQQEDLLS FGQLVISLTC DFFQPTLPLS LPLEHISRHY SSDLSNLILY LISKPAQGQV KSIDEVVKMM GPRILNELDA VQSYADVLEN ELGAEVENGR IVRLLTKLGF INERAEFELD PRWSDTGDRY ILKLFRDYVF HSVGVDGKPI LDLSHVLVCL NKLDAGLDER VMLVSRDDQS CLVVSYREIK HCIEAAFNEL KNAGNNHRVH R
NT Sequence
atgctgcctc cgcccaaatc agctgcggtg cagatcgtcc gtccgccgtc gccgtcctcg gagaaggcaa aggagaagga gaagaaacac tcgcccgaga agcgcgagac cccccagagg atatgccgca atgtcatgat ctacggctac tgcaagtacc aggaccaggg ttgcatctac taccatccac cggctggagc agacccatcc acgccccagg aaagcagccc agccacccac gcccccaccc cgctcgccgg cacaccagcc agagagaagc cgaccttgag catcgaacat ctcgccgcgc ccgtattcgt cccaaagggt ctcgactcct cctctccaag agtggccacc ccaagcgcac cgaccccgag tgctcccacg cctcccgtct ggccgtccct tccctcgacg ggacttttac ctcgccagga cgtccacgta tccgcccggc ccagccatgc ccagctgagc gctacggcgt ccccgatggc atacgatgac ccctcgcata tcgcgctctc cgccgcccat gctcacgccc aggcgcaagc actgacgcac agtatgcttg acccgcacgc ccatgcacct cccgtcgacc aaagcatgta cctcccaccc cgtcagccgc tcgactataa cctctacgca gcaccgctcc cgagcatcgg tgggaacccg ctttacccag cccatccgca cgcgttcttc gtgaacgatg atttgagaag atcaatccag gcgaagcagg aggctgttta tgtaggggcg aatggcgcct ccgcacccgg gctgccgcaa gagctgggcg tgtatcactc tctcgttcca cttcccctcc ccgctcctac cgcccaacgc ctccctaccc aatcccaacc ctccaaagta tacggcctgc cttcccccgt ctatcgcgcc acttctgaag tggatgggaa tacgtattgc ctgcgaaggg ttgaagggtt caagttggtc aaccagcttg catttgcatc gatggatacc tggagacgga tgaggcatcc aaacattgtc gggctaaagg aagcgtttac tacaaaaacg tttggagata actcgctaat catggtgtat gattaccacc ccctctctac aaccctctac gacgaatacc tctcacccaa ccccccggaa ccatctccag cttccctcct cgccggtaac caacctccca aacgacgttc atcgccaccc gagcggatcc tctggtccta tgtcacccaa atcgccaacg ccctcaaggc aatccacagt tccggcctcg ccgtccggaa cctcgatgcg agcaagattt tgttgaccgg gaagaacagg atcaggctga atggttgtgg agtgtgggac gtgttggcgt ttgataacaa gacccctgtg caggcattcc aacaggaaga tctgctctct tttggacaac tcgttatttc ccttacttgc gactttttcc aacctacact ccctctttcc cttccactcg aacacatctc cagacattac tcctccgatc tcagtaatct catattgtac ctgatcagca agccggcaca ggggcaagtc aagagtatcg atgaggtggt caagatgatg gggcctagga tattaaacga gctggatgct gttcagagtt atgcggatgt attggagaat gagttggggg ccgaagtgga gaatggacgt atcgtgagac tgctgaccaa gctgggcttt atcaatgagc gagcagagtt tgaattggac ccgcgttggt ctgatacggg cgataggtac atcctcaaac ttttcaggga ttatgtcttc catagcgttg gagtggatgg aaagccaatt ttggatctta gccatgtgtt ggtttgtttg aacaagttgg acgccggttt ggacgaaagg gtcatgctcg tctcacggga tgatcaaagt tgtcttgtcg ttagctatag agaaattaag cattgtattg aggctgcttt caacgagctg aagaatgcgg gcaataacca ccgagtccat cgatag
Details for CrneC.19379.a.B2.GE41074
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMD DLRRSIQAKQ EAVYVGANGA SAPGLPQELG VYHSLVPLPL PAPTAQRLPT QSQPSKVYGL PSPVYRATSE VDGNTYCLRR VEGFKLVNQL AFASMDTWRR MRHPNIVGLK EAFTTKTFGD NSLIMVYDYH PLSTTLYDEY LSPNPPEPSP ASLLAGNQPP KRRSSPPERI LWSYVTQIAN ALKAIHSSGL AVRNLDASKI LLTGKNRIRL NGCGVWDVLA FDNKTPVQAF QQEDLLSFGQ LVISLTCDFF QPTLPLSLPL EHISRHYSSD LSNLILYLIS KPAQGQVKSI DEVVKMMGPR ILNELDAVQS YADVLENELG AEVENGRIVR LLTKLGFINE RAEFELDPRW SDTGDRYILK LFRDYVFHSV GVDGKPILDL SHVLVCLNKL DAGLDERVML VSRDDQSCLV VSYREIKHCI EAA
Validated NT Sequence
gaaagcagcc tcaatacaat gcttaatttc tctatagcta acgacaagac aactttgatc atcccgtgag acgagcatga ccctttcgtc caaaccggcg tccaacttgt tcaaacaaac caacacatgg ctaagatcca aaattggctt tccatccact ccaacgctat ggaagacata atccctgaaa agtttgagga tgtacctatc gcccgtatca gaccaacgcg ggtccaattc aaactctgct cgctcattga taaagcccag cttggtcagc agtctcacga tacgtccatt ctccacttcg gcccccaact cattctccaa tacatccgca taactctgaa cagcatccag ctcgtttaat atcctaggcc ccatcatctt gaccacctca tcgatactct tgacttgccc ctgtgccggc ttgctgatca ggtacaatat gagattactg agatcggagg agtaatgtct ggagatgtgt tcgagtggaa gggaaagagg gagtgtaggt tggaaaaagt cgcaagtaag ggaaataacg agttgtccaa aagagagcag atcttcctgt tggaatgcct gcacaggggt cttgttatca aacgccaaca cgtcccacac tccacaacca ttcagcctga tcctgttctt cccggtcaac aaaatcttgc tcgcatcgag gttccggacg gcgaggccgg aactgtggat tgccttgagg gcgttggcga tttgggtgac ataggaccag aggatccgct cgggtggcga tgaacgtcgt ttgggaggtt ggttaccggc gaggagggaa gctggagatg gttccggggg gttgggtgag aggtattcgt cgtagagggt tgtagagagg gggtggtaat catacaccat gattagcgag ttatctccaa acgtttttgt agtaaacgct tcctttagcc cgacaatgtt tggatgcctc atccgtctcc aggtatccat cgatgcaaat gcaagctggt tgaccaactt gaacccttca acccttcgca ggcaatacgt attcccatcc acttcagaag tggcgcgata gacgggggaa ggcaggccgt atactttgga gggttgggat tgggtaggga ggcgttgggc ggtaggagcg gggaggggaa gtggaacgag agagtgatac acgcccagct cttgcggcag cccgggtgcg gaggcgccat tcgcccctac ataaacagcc tcctgcttcg cctggattga tcttctcaaa tcatccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHDD LRRSIQAKQE AVYVGANGAS APGLPQELGV YHSLVPLPLP APTAQRLPTQ SQPSKVYGLP SPVYRATSEV DGNTYCLRRV EGFKLVNQLA FASMDTWRRM RHPNIVGLKE AFTTKTFGDN SLIMVYDYHP LSTTLYDEYL SPNPPEPSPA SLLAGNQPPK RRSSPPERIL WSYVTQIANA LKAIHSSGLA VRNLDASKIL LTGKNRIRLN GCGVWDVLAF DNKTPVQAFQ QEDLLSFGQL VISLTCDFFQ PTLPLSLPLE HISRHYSSDL SNLILYLISK PAQGQVKSID EVVKMMGPRI LNELDAVQSY ADVLENELGA EVENGRIVRL LTKLGFINER AEFELDPRWS DTGDRYILKL FRDYVFHSVG VDGKPILDLS HVLVCLNKLD AGLDERVMLV SRDDQSCLVV SYREIKHCIE AAFNELKNAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgatgatt tgagaagatc aatccaggcg aagcaggagg ctgtttatgt aggggcgaat ggcgcctccg cacccgggct gccgcaagag ctgggcgtgt atcactctct cgttccactt cccctccccg ctcctaccgc ccaacgcctc cctacccaat cccaaccctc caaagtatac ggcctgcctt cccccgtcta tcgcgccact tctgaagtgg atgggaatac gtattgcctg cgaagggttg aagggttcaa gttggtcaac cagcttgcat ttgcatcgat ggatacctgg agacggatga ggcatccaaa cattgtcggg ctaaaggaag cgtttactac aaaaacgttt ggagataact cgctaatcat ggtgtatgat taccaccccc tctctacaac cctctacgac gaatacctct cacccaaccc cccggaacca tctccagctt ccctcctcgc cggtaaccaa cctcccaaac gacgttcatc gccacccgag cggatcctct ggtcctatgt cacccaaatc gccaacgccc tcaaggcaat ccacagttcc ggcctcgccg tccggaacct cgatgcgagc aagattttgt tgaccgggaa gaacaggatc aggctgaatg gttgtggagt gtgggacgtg ttggcgtttg ataacaagac ccctgtgcag gcattccaac aggaagatct gctctctttt ggacaactcg ttatttccct tacttgcgac tttttccaac ctacactccc tctttccctt ccactcgaac acatctccag acattactcc tccgatctca gtaatctcat attgtacctg atcagcaagc cggcacaggg gcaagtcaag agtatcgatg aggtggtcaa gatgatgggg cctaggatat taaacgagct ggatgctgtt cagagttatg cggatgtatt ggagaatgag ttgggggccg aagtggagaa tggacgtatc gtgagactgc tgaccaagct gggctttatc aatgagcgag cagagtttga attggacccg cgttggtctg atacgggcga taggtacatc ctcaaacttt tcagggatta tgtcttccat agcgttggag tggatggaaa gccaattttg gatcttagcc atgtgttggt ttgtttgaac aagttggacg ccggtttgga cgaaagggtc atgctcgtct cacgggatga tcaaagttgt cttgtcgtta gctatagaga aattaagcat tgtattgagg ctgctttcaa cgagctgaag aatgcgggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat