SSGCID: Target Page

CrneC.19376.a

Atypical/ABC1/ABC1-B protein kinase

CENTER ID:	CrneC.19376.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19376.a.B2.GE41181	kinase-like domain		254	516

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_04843
UniProt:	J9VSD9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLFLLTTGGQ PITTRAFQRS FHGALGGSVA ATHRPLLARP LTNRTTPNGV LSTLKTSNPA HQRGFETEKR RQNAIHMEVR LPVVKRSLYT SRISRNTLQP NTSPIPLTYL SSSPRRLRRL RNLLILLSLL SILYYTYPPF RHTAIAVVRC ARVMKAVSID VWDYKQVFAA EERLGASGKG LTDEEIEIKR KARRACHKRS AERLLEALKK NSGIYVKLGQ HVAAVQVLPK EWTETMRPLQ DQCFPTPVQR TDEMLRADLG MGIDDMFTDF EPNPIGVASL AQVHRGVDKR TGRAVAVKVQ HADLQEFAAV DMATVNFAIH FVKYVFPDFE FSWLGEEMNE MLPLEMDFRH EAANSARCMG DFLHLKGKTS LYLPEVFWAE RRCMVMEFIE GGRVDDLMYL KKHKIDRNQV SQELARIFSQ MVYINGYFHA DPHHGNLLIR PKASGSTSPF NFDVCLLDHG QYFDVPDDLR VNYAHFWLSL IKSTSKKTIA ERRHYARLVG NIDDDMYPIL ESAITGQINM ADESNTHDSA NDPRPTSLLD SKTFDKDQIR KLRAAMLERE GLIASIFELL RIVPRRMLMI LKLSDLQRSL DQSLATTHGQ SRVFVIVARY CAKAIWQADY ANFRKSLSTQ GFSVSLFKSF INSFFDYAYW NTTLGLVELG LDARARSIKI VLWFDGLVKG GLKAAEAEMA GLSSDGAIPA TA

NT Sequence

atgcttttcc tccttaccac aggaggacag cccatcacca cgagagcttt tcagcgctcc ttccatggag cattgggtgg ttcagtggcg gcgacccatc gaccactcct cgcgcgacca ctgacaaacc gaacgacgcc gaatggcgtg ttgtcgacat tgaaaacttc aaatccggcc catcaaagag gtttcgagac tgaaaagaga cgtcagaatg ctatccatat ggaagtacgg ttaccagtag tcaagaggag cttgtacact tctcgaatct cgcggaatac ccttcaaccc aacacttccc ccattccact cacttatcta tccagttctc ctcgtcgtct tcgcagactc cgaaatttgc tcatcctcct ctcccttctc tctatactct attacacata tccccccttt cgccatacag ctatcgctgt cgtccgttgc gcaagagtga tgaaagcagt ctctatcgat gtatgggatt acaagcaggt ttttgctgcc gaagagcgct taggagcgtc aggcaagggg ctcacggatg aggagattga aattaagaga aaggctagaa gagcctgtca caagcgcagt gcggagagat tgttggaggc gcttaagaag aattcgggta tttatgtaaa actggggcaa catgttgctg ctgtacaagt tttaccgaaa gaatggacag aaacgatgag gccattgcaa gaccagtgtt tcccgacacc tgtgcagaga acggatgaga tgttacgtgc ggatctgggg atggggatcg acgacatgtt tacagacttt gagcccaatc ctatcggagt tgcctccctt gctcaggttc atcgtggagt cgacaagcgc acaggtcggg ccgtagccgt caaagtccaa catgccgatt tacaagaatt tgccgctgtg gacatggcca ccgtcaattt tgcgatccat tttgtgaaat acgtcttccc cgattttgag ttcagttggt taggggagga gatgaacgag atgttgccgc tggagatgga ctttaggcat gaagctgcca actcggccag atgtatgggc gacttcttgc acctcaaggg gaagactagt ctctatcttc ccgaagtttt ctgggcagag aggaggtgta tggtcatgga gtttatcgag ggcggacgag tcgatgattt gatgtacctc aagaaacaca agattgaccg taatcaagta tcccaagaac tcgccaggat tttcagccag atggtgtata tcaatggcta tttccacgcc gaccctcatc atggtaatct tctgattcga ccaaaagctt caggttccac atcgcctttc aatttcgacg tctgtctcct cgatcatgga cagtactttg acgtgcctga tgacctccga gtgaattatg cccacttttg gttatccctt attaaatcca cgtccaaaaa gacaattgcc gaaagacggc attatgctag acttgttggg aacattgatg atgatatgta ccccatattg gaatccgcca ttaccggcca aatcaacatg gctgatgaaa gcaacaccca cgattcagct aacgatccta gacccacttc attgctcgat tccaagacat ttgacaagga tcagatcaga aagttgaggg ctgctatgtt ggaaagggag ggattgattg cttccatctt tgaactctta cgaatagttc ctaggcgtat gttgatgatt ctcaaattgt cagacttgca acggtcactg gaccagagtc ttgctaccac ccatggacaa agccgagtct ttgttatcgt cgctcgctac tgtgccaagg ctatctggca agccgactat gccaactttc gcaaatcgct ctctacccag ggcttttctg tgagcctgtt caaaagcttt atcaactcat tcttcgatta tgcgtactgg aatactacac tgggtttagt ggaacttggg ttggatgctc gagcgaggtc catcaagatt gtgctatggt tcgatggttt ggtcaagggc ggtctgaaag cggcggaagc tgaaatggcg ggtttatcgt cggatggtgc gatacctgcg acagcatag

Details for CrneC.19376.a.B2.GE41181

HARVESTED ON:	3/1/2017
SEQUENCED ON:	3/2/2017
EXPECTED MW:	31kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHML RADLGMGIDD MFTDFEPNPI GVASLAQVHR GVDKRTGRAV AVKVQHADLQ EFAAVDMATV NFAIHFVKYV FPDFEFSWLG EEMNEMLPLE MDFRHEAANS ARCMGDFLHL KGKTSLYLPE VFWAERRCMV MEFIEGGRVD DLMYLKKHKI DRNQVSQELA RIFSQMVYIN GYFHADPHHG NLLIRPKASG STSPFNFDVC LLDHGQYFDV PDDLRVNYAH FWLSLIKSTS KKTIAERRHY ARLVGNIDDD MYPILESAIT G

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttac gtgcggatct ggggatgggg atcgacgaca tgtttacaga ctttgagccc aatcctatcg gagttgcctc ccttgctcag gttcatcgtg gagtcgacaa gcgcacaggt cgggccgtag ccgtcaaagt ccaacatgcc gatttacaag aatttgccgc tgtggacatg gccaccgtca attttgcgat ccattttgtg aaatacgtct tccccgattt tgagttcagt tggttagggg aggagatgaa cgagatgttg ccgctggaga tggactttag gcatgaagct gccaactcgg ccagatgtat gggcgacttc ttgcacctca aggggaagac tagtctctat cttcccgaag ttttctgggc agagaggagg tgtatggtca tggagtttat cgagggcgga cgagtcgatg atttgatgta cctcaagaaa cacaagattg accgtaatca agtatcccaa gaactcgcca ggattttcag ccagatggtg tatatcaatg gctatttcca cgccgaccct catcatggta atcttctgat tcgaccaaaa gcttcaggtt ccacatcgcc tttcaatttc gacgtctgtc tcctcgatca tggacagtac tttgacgtgc ctgatgacct ccgagtgaat tatgcccact tttggttatc ccttattaaa tccacgtcca aaaagacaat tgccgaaaga cggcattatg ctagacttgt tgggaacatt gatgatgata tgtaccccat attggaatcc gccattaccg gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat