SSGCID: Target Page

CrneC.19366.a

SCYL protein kinase

CENTER ID:	CrneC.19366.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19366.a.B2.GE41073	kinase domain		22	301
CrneC.19366.a.B3.GE41412	kinase domain and ARM repeat		22	586

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_04191
UniProt:	J9VRR0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNYLKSITTS VLQSTGVTFP FSVGERIPGL DSHSSIWEIR EGVKRDDGTP LTLFIYDSTL PPFQPGSRDR KVLFQLAKNA LKKLRTIRHP DIIRYIDSVE TETHIYIATE RVRPLEGVLR DWETGGALAS GAGRAKGKEN WIGWGVRSVS SALAFLNSPP LSQHHSYLIP TSIFVTPSLE WRLAGFELLT GKDDAGGILW GLGGVAPGDI GERCAPEVRK GGWSALRETD PAQHDTYLFA VLLFTLYNPH SPLPPLSSQP TPSSSGTLPK VLFPLWKRML NPNPRTRLTT ISLTDEAEKA GFWASNPFVS LVKGLDNFEL QSESDKLSLL RIIKEATTQA GTLPGPFTTY KILPSLLHSL SLPTAPSSAM LPLVLELSKL VPSSDYPKMV LDPVVRLYTS PDRGTRMALL EGLNEYVDKL DNKTVTEKIW PNLITGFADT VPVIREATIK AVYPLASKLS DRILNNDLLR VLAKMQMDTE PSIRTNTCIL LGRLAPILGH NTKKKVLVPA FARSLKDPFV HARVAGLMAL MASVECFDRE DLAGKVVPNM SFTLVDKEKL VRDQAFKAMN MFVSKLTDMV KDMPETALPS DQISSQGPVT TTSASISSNQ GGAVNSAAGA AGALAGWAIS SLGKQLKTSE AHSSMNSSGM PAGFGASAPT IKFNTASSSP AVPASSRVSE EIFRPVSASP SLKSVSPQPR AVNAFGRTTT NSSLSGTSGG MKLAGAKKAP GKSLADQLAG EWEEEDGGNA WGTDDLIDVN ADDDDWSAFE SAPVPEVVVP PPQPYYVSAP KPKPSSSNIT TSAALRSKPT LSPAISSTSS IPTHAPEPVK PASSTSTPLI PSSPALSTIS GAKSPVLSSQ VNTTDTERGQ MDSASASERS SPQLSVKAGN GTGTLQPNLA NMSKEEKEKE MARRREERKA KIAAMKEQKK SKA

NT Sequence

atgaattacc tcaaatccat caccacctcg gtacttcaat ctactggtgt cactttccca ttttcagttg gcgaacgtat acctgggctc gatagccata gttctatttg ggaaatcaga gagggcgtta aacgtgacga tggtaccccc ttaacactgt tcatctatga ttcgaccctt ccgccttttc aacccggcag cagggaccgt aaagtgctgt tccaactggc taagaatgct ctcaaaaagc tccgcacaat cagacaccct gacatcatac gatatatcga ctctgtggaa acagagacac atatctacat tgctaccgaa cgcgtgcgtc ctttagaagg ggttttgagg gactgggaaa caggaggtgc tcttgcgtca ggcgctggta gggcaaaagg caaggagaac tggatcgggt ggggtgtgag gagtgtctca tccgctcttg cttttctcaa ttctcctcct ctatcccaac atcattccta cctcattcca acctctatct ttgtcactcc ttccctcgaa tggcgtctag cgggatttga gcttctgaca ggtaaagatg atgctggagg catattatgg ggcctaggag gtgtggctcc gggcgatatc ggtgaaagat gtgcacctga agtgagaaag ggtggatgga gtgctctgag agaaaccgat cctgcccaac acgacaccta ccttttcgcc gtccttctct ttactttata caaccctcat tctcccttac cgcccctctc gtcgcagcct acaccttcgt cttccggcac acttcccaaa gttctcttcc cgttatggaa gcgaatgctc aatcctaatc ctcgtactcg gttgactaca atatctctca ctgatgaagc cgaaaaggct ggcttctggg catccaaccc atttgtcagc cttgtaaagg gtttggacaa cttcgagctg cagtctgaaa gtgataagct ttcgctattg cgaatcatca aagaggccac cacccaagct ggcacactcc caggaccgtt taccacttat aaaattcttc cgtcccttct tcactcctta tccctcccaa ccgcaccatc gtccgctatg cttccccttg ttcttgaatt aagcaagctc gttccttctt cagattatcc caagatggtc ctcgatccag tggtcagatt gtataccagt cctgacaggg gaacgaggat ggctttattg gaaggtttga atgagtatgt tgacaaactg gataacaaga cagtgacaga gaagatttgg ccaaatctta tcactggttt cgccgataca gtccccgtca tcagagaagc caccattaaa gcggtctatc cgttggcctc taagctttcg gaccgtattc tcaacaacga cctcctccgg gttctcgcca agatgcaaat ggacaccgag ccctccatac gaacaaacac atgcatcctt ctcggccgcc tcgcacccat cttgggccat aatactaaga agaaggtact tgtacctgcc tttgcgagga gtttgaagga tccatttgta catgctagag ttgcgggttt gatggcgtta atggccagcg tcgagtgttt tgatagagaa gatttggcgg gcaaggtggt tccgaatatg agttttacgt tggtcgataa ggagaagttg gtgagagatc aggcattcaa ggctatgaac atgtttgtct ccaagttgac agatatggtt aaagacatgc ctgaaactgc attaccatct gatcaaatct catcacaagg tcctgtcacc accacttctg cttcgatctc tagcaaccag ggaggagccg tcaacagtgc cgctggtgct gctggagccc ttgccgggtg ggccatctca agcttgggca agcagctcaa gacaagcgaa gctcattcca gtatgaacag ctctgggatg cctgcgggtt tcggtgcttc tgcccccacg atcaaattca ataccgctag ttcgagtccg gctgtaccag cttcttcaag agtcagtgag gaaatcttca gaccggtatc tgcatctccg tctctgaaaa gcgtttctcc tcaacctcgc gctgtcaacg cctttggccg aaccaccact aattccagct tgtcaggtac tagtggtgga atgaagttgg ccggcgcgaa aaaagcaccg gggaagagtc tggcggatca gcttgctggc gaatgggaag aagaggatgg cggcaacgct tgggggacgg atgacttgat tgatgttaat gctgatgacg atgattggtc cgctttcgaa tctgcgcccg ttccggaggt tgtagtaccg ccgccacaac catactatgt ttccgctccc aaacccaaac cttctagctc aaacatcacc acctctgccg ctttacgcag taaacctacc ctttctcctg caatttcctc aacatcctct attcctactc acgcacccga acctgtcaag cctgcttctt ctacttctac gcctctcata ccatccagcc ctgctctgtc gactatttcg ggtgctaaat ctcctgtgct cagttctcaa gttaatacaa ccgacacaga gaggggacaa atggactcag cctcagctag tgaaagaagt tcgcctcaac tttcagtcaa ggctggtaat ggcaccggaa cgcttcagcc caatctggcg aatatgagca aagaggagaa ggaaaaagag atggctagaa gaagagagga gagaaaggct aaaatcgcgg cgatgaaaga gcaaaagaag agcaaggcct ga

Details for CrneC.19366.a.B2.GE41073

HARVESTED ON:	2/8/2017
SEQUENCED ON:	2/13/2017
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS VGERIPGLDS HSSIWEIREG VKRDDGTPLT LFIYDSTLPP FQPGSRDRKV LFQLAKNALK KLRTIRHPDI IRYIDSVETE THIYIATERV RPLEGVLRDW ETGGALASGA GRAKGKENWI GWGVRSVSSA LAFLNSPPLS QHHSYLIPTS IFVTPSLEWR LAGFELLTGK DDAGGILWGL GGVAPGDIGE RCAPEVRKGG WSALRETDPA QHDTYLFAVL LFTLYNPHSP LPPLSSQPTP SSSGTLPKVL FPLWKRMLNP NPRTRLTTIS LTDEAEKAG

Validated NT Sequence

atcttactca cttagccagc cttttcggct tcatcagtga gagatattgt agtcaaccga gtacgaggat taggattgag cattcgcttc cataacggga agagaacttt gggaagtgtg ccggaagacg aaggtgtagg ctgcgacgag aggggcggta agggagaatg agggttgtat aaagtaaaga gaaggacggc gaaaaggtag gtgtcgtgtt gggcaggatc ggtttctctc agagcactcc atccaccctt tctcacttca ggtgcacatc tttcaccgat atcgcccgga gccacacctc ctaggcccca taatatgcct ccagcatcat ctttacctgt cagaagctca aatcccgcta gacgccattc gagggaagga gtgacaaaga tagaggttgg aatgaggtag gaatgatgtt gggatagagg aggagaattg agaaaagcaa gagcggatga gacactcctc acaccccacc cgatccagtt ctccttgcct tttgccctac cagcgcctga cgcaagagca cctcctgttt cccagtccct caaaacccct tctaaaggac gcacgcgttc ggtagcaatg tagatatgtg tctctgtttc cacagagtcg atatatcgta tgatgtcagg gtgtctgatt gtgcggagct ttttgagagc attcttagcc agttggaaca gcactttacg gtccctgctg ccgggttgaa aaggcggaag ggtcgaatca tagatgaaca gtgttaaggg ggtaccatcg tcacgtttaa cgccctctct gatttcccaa atagaactat ggctatcgag cccaggtata cgttcgccaa ctgacatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcagttg gcgaacgtat acctgggctc gatagccata gttctatttg ggaaatcaga gagggcgtta aacgtgacga tggtaccccc ttaacactgt tcatctatga ttcgaccctt ccgccttttc aacccggcag cagggaccgt aaagtgctgt tccaactggc taagaatgct ctcaaaaagc tccgcacaat cagacaccct gacatcatac gatatatcga ctctgtggaa acagagacac atatctacat tgctaccgaa cgcgtgcgtc ctttagaagg ggttttgagg gactgggaaa caggaggtgc tcttgcgtca ggcgctggta gggcaaaagg caaggagaac tggatcgggt ggggtgtgag gagtgtctca tccgctcttg cttttctcaa ttctcctcct ctatcccaac atcattccta cctcattcca acctctatct ttgtcactcc ttccctcgaa tggcgtctag cgggatttga gcttctgaca ggtaaagatg atgctggagg catattatgg ggcctaggag gtgtggctcc gggcgatatc ggtgaaagat gtgcacctga agtgagaaag ggtggatgga gtgctctgag agaaaccgat cctgcccaac acgacaccta ccttttcgcc gtccttctct ttactttata caaccctcat tctcccttac cgcccctctc gtcgcagcct acaccttcgt cttccggcac acttcccaaa gttctcttcc cgttatggaa gcgaatgctc aatcctaatc ctcgtactcg gttgactaca atatctctca ctgatgaagc cgaaaaggct ggctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for CrneC.19366.a.B3.GE41412

HARVESTED ON:	data unavailable
SEQUENCED ON:	4/27/2017
EXPECTED MW:	63kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	89
PERCENT COVERAGE:	52

Validated AA Sequence

Validated NT Sequence

atggctcacc accaccacca tcatatgtca gttggcgaac gtatacctgg gctcgatagc catagttcta tttgggaaat cagagagggc gttaaacgtg acgatggtac ccccttaaca ctgttcatct atgattcgac ccttccgcct tttcaacccg gcagcaggga ccgtaaagtg ctgttccaac tggctaagaa tgctctcaaa aagctccgca caatcagaca ccctgacatc atacgatata tcgactctgt ggaaacagag acacatatct acattgctac cgaacgcgtg cgtcctttag aaggggtttt gagggactgg gaaacaggag gtgctcttgc gtcaggcgct ggtagggcaa aaggcaagga gaactggatc gggtggggtg tgaggagtgt ctcatccgct cttgcttttc tcaattctcc tcctctatcc caacatcatt cctacctcat tccaacctct atctttgtca ctccttccct cgaatggcgt ctagcgggat ttgagcttct gacaggtaaa gatgatgctg gaggcatatt atggggcctn ggaggtgtgg ctccgggcga tatcggtgaa agatgtgcac ctgaagtgag aaagggtgga tggagtgctc tgagagaaac cgatcctgcc caacacgaca cctacctttt cgccgtcctt ctctttactt tatacaaccc tcattctccc ttaccgcccc tctcgtcgca gcctacacct tcgtcttccg gcacacttcc nnaagttctc ttcccgttnn nnnnnnnnnn nnncaatcnn nnnnnnnnta nncggtnnnn nnnnnnnnnn nnnnnnnntg aagccgaaaa ngctggcttc tnnnnnntcc aacccantnn nnnnnnnntg taaagggttt gnnnancttc gagctgcagt ctgaaagtga taagctttcg cnattgcgaa tcatcaaaga ggccaccacc caagctggca cactcccagg accgtttacc acttataaaa ttcttccgtc ccttcttcac tccttatccc tcccaaccgc accatcgtcc gctatgcttc cccttgttct tgaattaagc aagctcgttc cttcttcaga ttatcccaag atggtcctcg atccagtggt cagattgtat accagtcctg acaggggaac gaggatggct ttattggaag gtttgaatga gtatgttgac aaactggata acaagacagt gacagagaag atttggccaa atcttatcac tggtttcgcc gatacagtcc ccgtcatcag agaagccacc attaaagcgg tctatccgtt ggccnntaag ctttcggacc gtattctcaa caacgacctc ctccgggttc tcgccaagat gcaaatggac accgagccct ccatacgaac aaacacatgc atccttctcg gccgcctcgc acccatcttg ggccataata ctaagaagaa ggtacttgta cctgcctttg cgaggagttt gaaggatcca tttgtacatg ctagagttgc gggtttgatg gcgttaatgg ccagcgtcga gtgttttgat agagaagatt tggcgggcaa ggtggttccg aatatgagtt ttacgttggt cgataaggag aagttggtga gagatcaggc attnnnngct atgaacatgc ttgtctccaa gttgaca

Expected Protein Sequence

MAHHHHHHSV GERIPGLDSH SSIWEIREGV KRDDGTPLTL FIYDSTLPPF QPGSRDRKVL FQLAKNALKK LRTIRHPDII RYIDSVETET HIYIATERVR PLEGVLRDWE TGGALASGAG RAKGKENWIG WGVRSVSSAL AFLNSPPLSQ HHSYLIPTSI FVTPSLEWRL AGFELLTGKD DAGGILWGLG GVAPGDIGER CAPEVRKGGW SALRETDPAQ HDTYLFAVLL FTLYNPHSPL PPLSSQPTPS SSGTLPKVLF PLWKRMLNPN PRTRLTTISL TDEAEKAGFW ASNPFVSLVK GLDNFELQSE SDKLSLLRII KEATTQAGTL PGPFTTYKIL PSLLHSLSLP TAPSSAMLPL VLELSKLVPS SDYPKMVLDP VVRLYTSPDR GTRMALLEGL NEYVDKLDNK TVTEKIWPNL ITGFADTVPV IREATIKAVY PLASKLSDRI LNNDLLRVLA KMQMDTEPSI RTNTCILLGR LAPILGHNTK KKVLVPAFAR SLKDPFVHAR VAGLMALMAS VECFDREDLA GKVVPNMSFT LVDKEKLVRD QAFKAMNMFV SKLTDMVKDM PET

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcagttg gcgaacgtat acctgggctc gatagccata gttctatttg ggaaatcaga gagggcgtta aacgtgacga tggtaccccc ttaacactgt tcatctatga ttcgaccctt ccgccttttc aacccggcag cagggaccgt aaagtgctgt tccaactggc taagaatgct ctcaaaaagc tccgcacaat cagacaccct gacatcatac gatatatcga ctctgtggaa acagagacac atatctacat tgctaccgaa cgcgtgcgtc ctttagaagg ggttttgagg gactgggaaa caggaggtgc tcttgcgtca ggcgctggta gggcaaaagg caaggagaac tggatcgggt ggggtgtgag gagtgtctca tccgctcttg cttttctcaa ttctcctcct ctatcccaac atcattccta cctcattcca acctctatct ttgtcactcc ttccctcgaa tggcgtctag cgggatttga gcttctgaca ggtaaagatg atgctggagg catattatgg ggcctaggag gtgtggctcc gggcgatatc ggtgaaagat gtgcacctga agtgagaaag ggtggatgga gtgctctgag agaaaccgat cctgcccaac acgacaccta ccttttcgcc gtccttctct ttactttata caaccctcat tctcccttac cgcccctctc gtcgcagcct acaccttcgt cttccggcac acttcccaaa gttctcttcc cgttatggaa gcgaatgctc aatcctaatc ctcgtactcg gttgactaca atatctctca ctgatgaagc cgaaaaggct ggcttctggg catccaaccc atttgtcagc cttgtaaagg gtttggacaa cttcgagctg cagtctgaaa gtgataagct ttcgctattg cgaatcatca aagaggccac cacccaagct ggcacactcc caggaccgtt taccacttat aaaattcttc cgtcccttct tcactcctta tccctcccaa ccgcaccatc gtccgctatg cttccccttg ttcttgaatt aagcaagctc gttccttctt cagattatcc caagatggtc ctcgatccag tggtcagatt gtataccagt cctgacaggg gaacgaggat ggctttattg gaaggtttga atgagtatgt tgacaaactg gataacaaga cagtgacaga gaagatttgg ccaaatctta tcactggttt cgccgataca gtccccgtca tcagagaagc caccattaaa gcggtctatc cgttggcctc taagctttcg gaccgtattc tcaacaacga cctcctccgg gttctcgcca agatgcaaat ggacaccgag ccctccatac gaacaaacac atgcatcctt ctcggccgcc tcgcacccat cttgggccat aatactaaga agaaggtact tgtacctgcc tttgcgagga gtttgaagga tccatttgta catgctagag ttgcgggttt gatggcgtta atggccagcg tcgagtgttt tgatagagaa gatttggcgg gcaaggtggt tccgaatatg agttttacgt tggtcgataa ggagaagttg gtgagagatc aggcattcaa ggctatgaac atgtttgtct ccaagttgac agatatggtt aaagacatgc ctgaaacttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat