CrneC.19360.a

Uridine kinase Urk1 (EC 2.7.1.48)

CENTER ID: CrneC.19360.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
CrneC.19360.a.B1.GE41069 full length 1 578
CrneC.19360.a.B2.GE41070 kinase domain 1 27 252
CrneC.19360.a.B3.GE41071 kinase domain 2 254 505
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_03367
UniProt: J9VQ45

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEQTAGHRTP RTQQHFVYDP TQPQSKNQVL ISHGRAPWYG PDGRNVEAYV VGIAGGSASG KTSVARAILS ALNYIPTVLI LSQDSFYNAH SPEEVELAFK NDLDLDHPDA IDMTLFAQCI KDLKQGKATE IPVYSFHHHQ RMSEKKYIYG ASVIIVEGIM ALQSAELREL YDLKVFVNCD SDLMLARRIK RDVKERGRDV EGILDQYLRF VKSSYDTFVQ PSSRYADIIV PGSSNQLAIE LLVSHIKRQL ESRSLRFRRV LADIGENRGS STPSVEKFDK QIVLLEQRNQ LRGIMTILRD RTTCREEFIF HIDRLSTIIV EKALTLVPCE PKVVKTPNKN IYKGISQTNN LVGVSILRSG LPFSQGLRRV IRDVPIGGIL IQSDPKTGEP LLLKSDLPHC LRSRETNGDV RCLLLDSQMG TGAAAMMAIR VLLDHGISQD RIIFLTYLIS RSASYSVLRA FPNIQIVTAA IDPGLDEVKI PYMPGSLIMG EAAGEGDFAV RLVDQLGHEE DKKGDRVKDL LKTDEEMAAD GFKMNILKGT EELKFSRKHK RTHSPTGEKR AWVISPGMGH VGDRYYLV
NT Sequence
atggaacaga cagcaggaca tcgcactccc aggacacaac aacactttgt ctacgacccc acgcagccgc agagcaaaaa ccaagtcctg atatcccacg gacgggcacc ctggtacggc cctgatggca ggaatgtcga ggcatatgtg gtgggtattg ccggaggtag tgcgtccgga aagacttctg tagcgcgggc tatcctttcg gccctcaact acatccccac ggttcttatc ttgtcccagg attcgtttta caatgctcat tccccagaag aggtcgaact ggctttcaaa aatgacttgg acctcgacca tcccgacgcc attgacatga cgctgtttgc tcagtgcatc aaggacctga agcaaggcaa agctacagag atcccggtct actctttcca tcatcatcaa cgtatgtcgg agaaaaagta tatctacggt gcaagcgtca tcattgttga aggtatcatg gctcttcaat cagcagagtt gagggaattg tacgacctca aggtctttgt gaattgcgat tcagatctca tgctagcaag acgtataaag cgagatgtca aggaacgagg gagggatgtg gaaggcatcc tcgaccagta tttgaggttt gttaaaagca gctacgatac ttttgtccag ccttcatcgc gctatgcgga tattattgtt ccggggtcct caaatcaact tgccatcgaa cttcttgtct cgcatatcaa gcgccaactc gagtctcgtt ctcttcggtt ccgtagagtg ttagccgata tcggtgaaaa ccgcggcagc agcactcctt cagtagaaaa gtttgacaag cagattgttt tgcttgagca aagaaaccag ctgcgcggta tcatgacaat acttcgagat cgtaccacgt gccgagagga attcatcttc catatcgatc gtttatcaac cattatcgtg gagaaggccc ttacacttgt tccgtgtgag cctaaggttg tgaaaacacc gaataaaaat atttacaaag gtatttccca gaccaacaat cttgtggggg tatccattct tcgctctggt cttccgttct ctcaaggtct tcgtcgggtc atccgtgatg tgcctatcgg tggtatactc atccagtccg atcccaagac tggtgagcca ttgttattga aaagtgatct ccctcattgc ttgaggtcac gggagacaaa tggagacgtc aggtgtttgc tgttggattc gcagatgggt acgggagcgg ctgctatgat ggccatccgc gtactccttg accatggcat ttctcaggat cgtatcatat tcctcacata cctcatttct cgctcagcct catattctgt cctgcgcgca ttcccgaata ttcaaattgt taccgcagct attgaccctg gcctggacga ggtgaagatt ccctacatgc caggaagtct tattatgggc gaggcggctg gtgagggtga tttcgcggta aggttagtcg atcagttggg acatgaagag gacaagaaag gggatagggt gaaagatctg ttgaagacgg atgaagagat ggctgcggat ggttttaaga tgaacatttt gaagggcaca gaggaattga aattttctag aaagcataaa aggacacatt caccaacagg agagaaaagg gcatgggtca tttctccagg catgggccat gttggagatc ggtattacct ggtttga
Details for CrneC.19360.a.B1.GE41069
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 66kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 94
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHME QTAGHRTPRT QQHFVYDPTQ PQSKNQVLIS HGRAPWYGPD GRNVEAYVVG IAGGSASGKT SVARAILSAL NYIPTVLILS QDSFYNAHSP EEVELAFKND LDLDHPDAID MTLFAQCIKD LKQGKATEIP VYSFHHHQRM SEKKYIYGAS VIIVEGIMAL QSAELRELYD LKVFVNCDSD LMLARRIKRD VKERGRDVEG ILDQYLRFVK SSYDTFVQPS SRYADIIVPG SXXQLAIELL VSHIKRQLXS RSLXXRXVLA DIGEXRGSST PXVEXXXXQI XXXXXXXXXX GIMTILRXRT TCRXXXXXHI DRLSTIIVEX XXTLVPCEPK VVKTPNKNIY KGISQTNNLV GVSILRSGLP FSQGLRRVIR DVPIGGILIQ SDPKTGEPLL LKSDLPHCLR SRETNGDVRC LLLDSQMGTG AAAMMAIRVL LDHGISQDRI IFLTYLISRS ASYSVLRAFP NIQIVTAAID PGLDEVKIPY MPGSLIMGEA AGEGDFAVRL VDQLGHEEDK KGDRVKDLLK TDEEMAADGF KMNILKGTEE LKFSRKHKRT HSPTGEKRAW VISPGMGH
Validated NT Sequence
catggcccat gcctggagaa atgacccatg cccttttctc tcctgttggt gaatgtgtcc ttttatgctt tctagaaaat ttcaattcct ctgtgccctt caaaatgttc atcttaaaac catccgcagc catctcttca tccgtcttca acagatcttt caccctatcc cctttcttgt cctcttcatg tcccaactga tcgactaacc ttaccgcgaa atcaccctca ccagccgcct cgcccataat aagacttcct ggcatgtagg gaatcttcac ctcgtccagg ccagggtcaa tagctgcggt aacaatttga atattcggga atgcgcgcag gacagaatat gaggctgagc gagaaatgag gtatgtgagg aatatgatac gatcctgaga aatgccatgg tcaaggagta cgcggatggc catcatagca gccgctcccg tacccatctg cgaatccaac agcaaacacc tgacgtctcc atttgtctcc cgtgacctca agcaatgagg gagatcactt ttcaataaca atggctcacc agtcttggga tcggactgga tgagtatacc accgataggc acatcacgga tgacccgacg aagaccttga gagaacggaa gaccagagcg aagaatggat acccccacaa gattgttggt ctgggaaata cctttgtaaa tatttttatt cggtgttttc acaaccttag gctcacacgg aacaagtgta annnnnntct ccacgataat ggttgataaa cgatcgatat ggannnnnaa nnnnnnncgg cacgtggtac gntctcgaag tattgtcatg ataccgnnnn nnnnnnnnnn nnncnnnnnn annnnaatct gcnnnnnnnn nttttctact naaggagtgc tgctgccgcg nntttcaccg atatcggcta acacnnnacg nnnnngaaga gaacgagact nnagttggcg cttgatatgc gagacaagaa gttcgatggc aagttgannn naggaccccg gaacaataat atccgcatag cgcgatgaag gctggacaaa agtatcgtag ctgcttttaa caaacctcaa atactggtcg aggatgcctt ccacatccct ccctcgttcc ttgacatctc gctttatacg tcttgctagc atgagatctg aatcgcaatt cacaaagacc ttgaggtcgt acaattccct caactctgct gattgaagag ccatgatacc ttcaacaatg atgacgcttg caccgtagat atactttttc tccgacatac gttgatgatg atggaaagag tagaccggga tctctgtagc tttgccttgc ttcaggtcct tgatgcactg agcaaacagc gtcatgtcaa tggcgtcggg atggtcgagg tccaagtcat ttttgaaagc cagttcgacc tcttctgggg aatgagcatt gtaaaacgaa tcctgggaca agataagaac cgtggggatg tagttgaggg ccgaaaggat agcccgcgct acagaagtct ttccggacgc actacctccg gcaataccca ccacatatgc ctcgacattc ctgccatcag ggccgtacca gggtgcccgt ccgtgggata tcaggacttg gtttttgctc tgcggctgcg tggggtcgta gacaaagtgt tgttgtgtcc tgggagtgcg atgtcctgct gtctgttcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHME QTAGHRTPRT QQHFVYDPTQ PQSKNQVLIS HGRAPWYGPD GRNVEAYVVG IAGGSASGKT SVARAILSAL NYIPTVLILS QDSFYNAHSP EEVELAFKND LDLDHPDAID MTLFAQCIKD LKQGKATEIP VYSFHHHQRM SEKKYIYGAS VIIVEGIMAL QSAELRELYD LKVFVNCDSD LMLARRIKRD VKERGRDVEG ILDQYLRFVK SSYDTFVQPS SRYADIIVPG SSNQLAIELL VSHIKRQLES RSLRFRRVLA DIGENRGSST PSVEKFDKQI VLLEQRNQLR GIMTILRDRT TCREEFIFHI DRLSTIIVEK ALTLVPCEPK VVKTPNKNIY KGISQTNNLV GVSILRSGLP FSQGLRRVIR DVPIGGILIQ SDPKTGEPLL LKSDLPHCLR SRETNGDVRC LLLDSQMGTG AAAMMAIRVL LDHGISQDRI IFLTYLISRS ASYSVLRAFP NIQIVTAAID PGLDEVKIPY MPGSLIMGEA AGEGDFAVRL VDQLGHEEDK KGDRVKDLLK TDEEMAADGF KMNILKGTEE LKFSRKHKRT HSPTGEKRAW VISPGMGHVG DRYYLV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaac agacagcagg acatcgcact cccaggacac aacaacactt tgtctacgac cccacgcagc cgcagagcaa aaaccaagtc ctgatatccc acggacgggc accctggtac ggccctgatg gcaggaatgt cgaggcatat gtggtgggta ttgccggagg tagtgcgtcc ggaaagactt ctgtagcgcg ggctatcctt tcggccctca actacatccc cacggttctt atcttgtccc aggattcgtt ttacaatgct cattccccag aagaggtcga actggctttc aaaaatgact tggacctcga ccatcccgac gccattgaca tgacgctgtt tgctcagtgc atcaaggacc tgaagcaagg caaagctaca gagatcccgg tctactcttt ccatcatcat caacgtatgt cggagaaaaa gtatatctac ggtgcaagcg tcatcattgt tgaaggtatc atggctcttc aatcagcaga gttgagggaa ttgtacgacc tcaaggtctt tgtgaattgc gattcagatc tcatgctagc aagacgtata aagcgagatg tcaaggaacg agggagggat gtggaaggca tcctcgacca gtatttgagg tttgttaaaa gcagctacga tacttttgtc cagccttcat cgcgctatgc ggatattatt gttccggggt cctcaaatca acttgccatc gaacttcttg tctcgcatat caagcgccaa ctcgagtctc gttctcttcg gttccgtaga gtgttagccg atatcggtga aaaccgcggc agcagcactc cttcagtaga aaagtttgac aagcagattg ttttgcttga gcaaagaaac cagctgcgcg gtatcatgac aatacttcga gatcgtacca cgtgccgaga ggaattcatc ttccatatcg atcgtttatc aaccattatc gtggagaagg cccttacact tgttccgtgt gagcctaagg ttgtgaaaac accgaataaa aatatttaca aaggtatttc ccagaccaac aatcttgtgg gggtatccat tcttcgctct ggtcttccgt tctctcaagg tcttcgtcgg gtcatccgtg atgtgcctat cggtggtata ctcatccagt ccgatcccaa gactggtgag ccattgttat tgaaaagtga tctccctcat tgcttgaggt cacgggagac aaatggagac gtcaggtgtt tgctgttgga ttcgcagatg ggtacgggag cggctgctat gatggccatc cgcgtactcc ttgaccatgg catttctcag gatcgtatca tattcctcac atacctcatt tctcgctcag cctcatattc tgtcctgcgc gcattcccga atattcaaat tgttaccgca gctattgacc ctggcctgga cgaggtgaag attccctaca tgccaggaag tcttattatg ggcgaggcgg ctggtgaggg tgatttcgcg gtaaggttag tcgatcagtt gggacatgaa gaggacaaga aaggggatag ggtgaaagat ctgttgaaga cggatgaaga gatggctgcg gatggtttta agatgaacat tttgaagggc acagaggaat tgaaattttc tagaaagcat aaaaggacac attcaccaac aggagagaaa agggcatggg tcatttctcc aggcatgggc catgttggag atcggtatta cctggtttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for CrneC.19360.a.B2.GE41070
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMN QVLISHGRAP WYGPDGRNVE AYVVGIAGGS ASGKTSVARA ILSALNYIPT VLILSQDSFY NAHSPEEVEL AFKNDLDLDH PDAIDMTLFA QCIKDLKQGK ATEIPVYSFH HHQRMSEKKY IYGASVIIVE GIMALQSAEL RELYDLKVFV NCDSDLMLAR RIKRDVKERG RDVEGILDQY LRFVKSSYDT FVQPSSRYAD IIVPGSSNQL AIELLVSHIK R
Validated NT Sequence
ttggcgcttg atatgcgaga caagaagttc gatggcaagt tgatttgagg accccggaac aataatatcc gcatagcgcg atgaaggctg gacaaaagta tcgtagctgc ttttaacaaa cctcaaatac tggtcgagga tgccttccac atccctccct cgttccttga catctcgctt tatacgtctt gctagcatga gatctgaatc gcaattcaca aagaccttga ggtcgtacaa ttccctcaac tctgctgatt gaagagccat gataccttca acaatgatga cgcttgcacc gtagatatac tttttctccg acatacgttg atgatgatgg aaagagtaga ccgggatctc tgtagctttg ccttgcttca ggtccttgat gcactgagca aacagcgtca tgtcaatggc gtcgggatgg tcgaggtcca agtcattttt gaaagccagt tcgacctctt ctggggaatg agcattgtaa aacgaatcct gggacaagat aagaaccgtg gggatgtagt tgagggccga aaggatagcc cgcgctacag aagtctttcc ggacgcacta cctccggcaa tacccaccac atatgcctcg acattcctgc catcagggcc gtaccagggt gcccgtccgt gggatatcag gacttggttc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHNQ VLISHGRAPW YGPDGRNVEA YVVGIAGGSA SGKTSVARAI LSALNYIPTV LILSQDSFYN AHSPEEVELA FKNDLDLDHP DAIDMTLFAQ CIKDLKQGKA TEIPVYSFHH HQRMSEKKYI YGASVIIVEG IMALQSAELR ELYDLKVFVN CDSDLMLARR IKRDVKERGR DVEGILDQYL RFVKSSYDTF VQPSSRYADI IVPGSSNQLA IELLVSHIKR QLES
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaccaag tcctgatatc ccacggacgg gcaccctggt acggccctga tggcaggaat gtcgaggcat atgtggtggg tattgccgga ggtagtgcgt ccggaaagac ttctgtagcg cgggctatcc tttcggccct caactacatc cccacggttc ttatcttgtc ccaggattcg ttttacaatg ctcattcccc agaagaggtc gaactggctt tcaaaaatga cttggacctc gaccatcccg acgccattga catgacgctg tttgctcagt gcatcaagga cctgaagcaa ggcaaagcta cagagatccc ggtctactct ttccatcatc atcaacgtat gtcggagaaa aagtatatct acggtgcaag cgtcatcatt gttgaaggta tcatggctct tcaatcagca gagttgaggg aattgtacga cctcaaggtc tttgtgaatt gcgattcaga tctcatgcta gcaagacgta taaagcgaga tgtcaaggaa cgagggaggg atgtggaagg catcctcgac cagtatttga ggtttgttaa aagcagctac gatacttttg tccagccttc atcgcgctat gcggatatta ttgttccggg gtcctcaaat caacttgcca tcgaacttct tgtctcgcat atcaagcgcc aactcgagtc ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for CrneC.19360.a.B3.GE41071
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS LRFRRVLADI GENRGSSTPS VEKFDKQIVL LEQRNQLRGI MTILRDRTTC REEFIFHIDR LSTIIVEKAL TLVPCEPKVV KTPNKNIYKG ISQTNNLVGV SILRSGLPFS QGLRRVIRDV PIGGILIQSD PKTGEPLLLK SDLPHCLRSR ETNGDVRCLL LDSQMGTGAA AMMAIRVLLD HGISQDRIIF LTYLISRSAS YSVLRAFPNI QIVTAAIDPG LDEVKIPYMP GSLIMGEAAG EGDFAXRLVD Q
Validated NT Sequence
atcttactca cttactgatc gactaacctt ancgcgaaat caccctcacc agccgcctcg cccataataa gacttcctgg catgtaggga atcttcacct cgtccaggcc agggtcaata gctgcggtaa caatttgaat attcgggaat gcgcgcagga cagaatatga ggctgagcga gaaatgaggt atgtgaggaa tatgatacga tcctgagaaa tgccatggtc aaggagtacg cggatggcca tcatagcagc cgctcccgta cccatctgcg aatccaacag caaacacctg acgtctccat ttgtctcccg tgacctcaag caatgaggga gatcactttt caataacaat ggctcaccag tcttgggatc ggactggatg agtataccac cgataggcac atcacggatg acccgacgaa gaccttgaga gaacggaaga ccagagcgaa gaatggatac ccccacaaga ttgttggtct gggaaatacc tttgtaaata tttttattcg gtgttttcac aaccttaggc tcacacggaa caagtgtaag ggccttctcc acgataatgg ttgataaacg atcgatatgg aagatgaatt cctctcggca cgtggtacga tctcgaagta ttgtcatgat accgcgcagc tggtttcttt gctcaagcaa aacaatctgc ttgtcaaact tttctactga aggagtgctg ctgccgcggt tttcaccgat atcggctaac actctacgga accgaagaga catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHSL RFRRVLADIG ENRGSSTPSV EKFDKQIVLL EQRNQLRGIM TILRDRTTCR EEFIFHIDRL STIIVEKALT LVPCEPKVVK TPNKNIYKGI SQTNNLVGVS ILRSGLPFSQ GLRRVIRDVP IGGILIQSDP KTGEPLLLKS DLPHCLRSRE TNGDVRCLLL DSQMGTGAAA MMAIRVLLDH GISQDRIIFL TYLISRSASY SVLRAFPNIQ IVTAAIDPGL DEVKIPYMPG SLIMGEAAGE GDFAVRLVDQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctcttc ggttccgtag agtgttagcc gatatcggtg aaaaccgcgg cagcagcact ccttcagtag aaaagtttga caagcagatt gttttgcttg agcaaagaaa ccagctgcgc ggtatcatga caatacttcg agatcgtacc acgtgccgag aggaattcat cttccatatc gatcgtttat caaccattat cgtggagaag gcccttacac ttgttccgtg tgagcctaag gttgtgaaaa caccgaataa aaatatttac aaaggtattt cccagaccaa caatcttgtg ggggtatcca ttcttcgctc tggtcttccg ttctctcaag gtcttcgtcg ggtcatccgt gatgtgccta tcggtggtat actcatccag tccgatccca agactggtga gccattgtta ttgaaaagtg atctccctca ttgcttgagg tcacgggaga caaatggaga cgtcaggtgt ttgctgttgg attcgcagat gggtacggga gcggctgcta tgatggccat ccgcgtactc cttgaccatg gcatttctca ggatcgtatc atattcctca catacctcat ttctcgctca gcctcatatt ctgtcctgcg cgcattcccg aatattcaaa ttgttaccgc agctattgac cctggcctgg acgaggtgaa gattccctac atgccaggaa gtcttattat gggcgaggcg gctggtgagg gtgatttcgc ggtaaggtta gtcgatcagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat