CrneC.19358.a

Haspin protein kinase

CENTER ID: CrneC.19358.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19358.a.B2.GE42501 kinase domain 127 504
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_01333
UniProt: J9VK17

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPPFSKSPRI ASTQQRRVSR KTSKAIVPRS PAAAHTTLPS APKTPMVASG QSTTQQRSPV TVIRAKKRLV FEGVILPLRS QISDIKIEEL SERLSDVTIK DSSAQKTGNG ANKEKPSAQF ALEALIQACS STSVQEFNTF IKSFPLFSAS KDVFVTKVGE ASYSEVFGFS QSAEDVDLVL KVIPLFLGTV ETDVPFPDCS SPEDVLREIE ITKKMNQVPG GGFVEFRGAY VVEGKYPNEL LEKWDIYKST QGSASVRPSA FGPTQKYCLV ALCNSGIDLE ALQFDASRGW VQAAGIFWQV AAALASAEDW TKFEHRDLHE GQILISSLSE FPSPTEPENY LSPTYTSLQT TIIDFGLSRL NMPTPVWSQI PEEVYEGKGA QWDLYRAMRS RIGDNWDGFH AITNLMWLRY ILQYMLVSKS LRKPRALKST IAPRLARGAK PKPDTVRSEK AWSMLQKVEE IMEYSLNFDM TSATSNRNHK NEALKPLTSA RDLVNWGKNE KWIG
NT Sequence
atgcctcctt tcagtaaatc tccacggata gcttcaacgc agcaaaggag ggtatcccgg aaaacctcaa aggccatcgt tcctaggagc cctgccgctg ctcatacaac actcccgtca gcacccaaaa caccgatggt ggcttcaggg cagtctacaa cgcagcagcg ctctccagtt acagttatca gggcgaagaa aaggcttgtc tttgagggag ttatactgcc tttacggtca cagataagcg acatcaaaat cgaggagtta tcagaaagac tatcggatgt gacaatcaaa gacagttctg ctcaaaaaac agggaatggc gcgaacaaag aaaagccatc agctcaattt gccttggaag ctctcataca agcatgttct tcaacctctg ttcaggagtt taacaccttt atcaaatcat tcccattatt ttctgcctcc aaagatgtct tcgtgaccaa agtgggcgaa gcatcctact ctgaggtatt tggcttttcc cagtcagccg aagatgtcga tctagtccta aaggtaatcc cgttgttttt ggggacggtg gagacagatg tgccatttcc cgactgtagc tcacccgagg atgttctgag ggaaattgaa attaccaaga agatgaacca agtaccaggt ggcggtttcg tggaattcag aggcgcgtat gtcgtggaag gaaagtatcc taatgaattg cttgaaaaat gggatatcta caaatccacc cagggctctg ctagtgtacg gccttctgca tttggcccaa cacagaaata ttgccttgtg gctctctgta attcagggat tgaccttgag gccctccagt ttgatgcttc tcggggttgg gtgcaggccg ctgggatatt ctggcaagta gcagcagctc tggcatctgc cgaggactgg acaaaatttg agcatcgtga tctccatgag ggccaaatcc ttatatcatc cttatccgaa tttccttccc ctacagaacc ggaaaactat ttgtcgccaa catacacatc tttgcaaacc actatcattg attttggtct ctcacgttta aacatgccca ccccagtttg gtcccaaata cctgaagagg tgtatgaagg caaaggagca caatgggatt tgtacagagc catgagatcg aggataggtg acaactggga tggtttccat gcaatcacga acctcatgtg gctgcgatat attcttcagt acatgctcgt atcgaaatct cttagaaagc cccgcgctct caaatctaca atagcccctc ggttggccag gggcgcgaaa ccaaaacccg ataccgtccg gtctgaaaaa gcttggagca tgctccagaa agtcgaggag atcatggaat acagtctgaa tttcgacatg acgagtgcaa cgtcaaatag gaatcacaag aacgaagcgt tgaaaccttt aacttcagcg agagacttgg tgaattgggg taaaaatgaa aaatggattg gatga
Details for CrneC.19358.a.B2.GE42501
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMQ ACSSTSVQEF NTFIKSFPLF SASKDVFVTK VGEASYSEVF GFSQSAEDVD LVLKVIPLFL GTVETDVPFP DCSSPEDVLR EIEITKKMNQ VPGGGFVEFR GAYVVEGKYP NELLEKWDIY KSTQGSASVR PSAFGPTQKY CLVALCNSGI DLEALQFDAS RGWVQAAGIF WQVAAALASA EDWTKFEHRD LHEGQILISS LSEFPSPTEP ENYLSPTYTS LQTTIIDFGL SRLNMPTPVW SQIPEEVYEG KGAQWDLYRA MRSRIGDNWD GFHAITNLMW LRYILQYMLV SKSLRKPRAL KSTIAPRLAR GAKPKPDTVR SEKAWSMLQK VEEIMEYSLN FDMTSATSNR NHKNEALKPL TSARDLVN
Validated NT Sequence
atggctcacc accaccacca ccatatgcaa gcatgttctt caacctctgt tcaggagttt aacaccttta tcaaatcatt cccattattt tctgcctcca aagatgtctt cgtgaccaaa gtgggcgaag catcctactc tgaggtattt ggcttttccc agtcagccga agatgtcgat ctagtcctaa aggtaatccc gttgtttttg gggacggtgg agacagatgt gccatttccc gactgtagct cacccgagga tgttctgagg gaaattgaaa ttaccaagaa gatgaaccaa gtaccaggtg gcggtttcgt ggaattcaga ggcgcgtatg tcgtggaagg aaagtatcct aatgaattgc ttgaaaaatg ggatatctac aaatccaccc agggctctgc tagtgtacgg ccttctgcat ttggcccaac acagaaatat tgccttgtgg ctctctgtaa ttcagggatt gaccttgagg ccctccagtt tgatgcttct cggggttggg tgcaggccgc tgggatattc tggcaagtag cagcagctct ggcatctgcc gaggactgga caaaatttga gcatcgtgat ctccatgagg gccaaatcct tatatcatcc ttatccgaat ttccttcccc tacagaaccg gaaaactatt tgtcgccaac atacacatct ttgcaaacca ctatcattga ttttggtctc tcacgtttaa acatgcccac cccagtttgg tcccaaatac ctgaagaggt gtatgaaggc aaaggagcac aatgggattt gtacagagcc atgagatcga ggataggtga caactgggat ggtttccatg caatcacgaa cctcatgtgg ctgcgatata ttcttcagta catgctcgta tcgaaatctc ttagaaagcc ccgcgctctc aaatctacaa tagcccctcg gttggccagg ggcgcgaaac caaaacccga taccgtccgg tctgaaaaag cttggagcat gctccagaaa gtcgaggaga tcatggaata cagtctgaat ttcgacatga cgagtgcaac gtcaaatagg aatcacaaga acgaagcgtt gaaaccttta acttcagcaa gagacttggt gaattg
Expected Protein Sequence
MAHHHHHHQA CSSTSVQEFN TFIKSFPLFS ASKDVFVTKV GEASYSEVFG FSQSAEDVDL VLKVIPLFLG TVETDVPFPD CSSPEDVLRE IEITKKMNQV PGGGFVEFRG AYVVEGKYPN ELLEKWDIYK STQGSASVRP SAFGPTQKYC LVALCNSGID LEALQFDASR GWVQAAGIFW QVAAALASAE DWTKFEHRDL HEGQILISSL SEFPSPTEPE NYLSPTYTSL QTTIIDFGLS RLNMPTPVWS QIPEEVYEGK GAQWDLYRAM RSRIGDNWDG FHAITNLMWL RYILQYMLVS KSLRKPRALK STIAPRLARG AKPKPDTVRS EKAWSMLQKV EEIMEYSLNF DMTSATSNRN HKNEALKPLT SARDLVNWGK NEKWIG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaagcat gttcttcaac ctctgttcag gagtttaaca cctttatcaa atcattccca ttattttctg cctccaaaga tgtcttcgtg accaaagtgg gcgaagcatc ctactctgag gtatttggct tttcccagtc agccgaagat gtcgatctag tcctaaaggt aatcccgttg tttttgggga cggtggagac agatgtgcca tttcccgact gtagctcacc cgaggatgtt ctgagggaaa ttgaaattac caagaagatg aaccaagtac caggtggcgg tttcgtggaa ttcagaggcg cgtatgtcgt ggaaggaaag tatcctaatg aattgcttga aaaatgggat atctacaaat ccacccaggg ctctgctagt gtacggcctt ctgcatttgg cccaacacag aaatattgcc ttgtggctct ctgtaattca gggattgacc ttgaggccct ccagtttgat gcttctcggg gttgggtgca ggccgctggg atattctggc aagtagcagc agctctggca tctgccgagg actggacaaa atttgagcat cgtgatctcc atgagggcca aatccttata tcatccttat ccgaatttcc ttcccctaca gaaccggaaa actatttgtc gccaacatac acatctttgc aaaccactat cattgatttt ggtctctcac gtttaaacat gcccacccca gtttggtccc aaatacctga agaggtgtat gaaggcaaag gagcacaatg ggatttgtac agagccatga gatcgaggat aggtgacaac tgggatggtt tccatgcaat cacgaacctc atgtggctgc gatatattct tcagtacatg ctcgtatcga aatctcttag aaagccccgc gctctcaaat ctacaatagc ccctcggttg gccaggggcg cgaaaccaaa acccgatacc gtccggtctg aaaaagcttg gagcatgctc cagaaagtcg aggagatcat ggaatacagt ctgaatttcg acatgacgag tgcaacgtca aataggaatc acaagaacga agcgttgaaa cctttaactt cagcgagaga cttggtgaat tggggtaaaa atgaaaaatg gattggatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at