SSGCID: Target Page

CrneC.19350.a

Dihydroxyacetone kinase

CENTER ID:	CrneC.19350.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19350.a.B2.GE41000	DhaK domain		1	345
CrneC.19350.a.B3.GE41001	DhaL domain		383	591

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_02799
UniProt:	J9VIA1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAPAQKHLLN TPQTLVVDSL KGLATLNPNV KLDEAQRVIY TPPTESKVAL LSGGGSGHEP AHAAFVGPGL LSAAICGNIF ASPNVAQIRR GLELVTREKG GLVVVMNYTG DALHFGLAAE QHRSAGKLGD VRVLMVGDDV AVGREQGSIV GRRGLAGTIL VYKVAAALSD KGADLDSVEN IAKYVASRLG TLGVGLEHCH VPGTRAGNSH LGANEVEVGM GIHNEAGTYK LDMTTASELV GKMLTQITDT TDKDKSFVPF KGDGSDEVVL LVNDLGAISE LEMGGLTNEA VKWLQSRKIK VRRVLAGTYM TSLNMPGFSL TLLLLPPASE KAPYSADEIL EYLDTPASAP GWKWHAGREP GTLDVKAEEA SIPVQKDNAV LPSTDAKGFL AAIARACKAL IAAEPELTEQ DQIAGDGDAG LTLEAGAKGV LKAIEEGRLK GENVVQDVKV IAEIVEEDMG GTSGALYSIF FAGLGKSLRD AATEGAKSTS PEVWSKAAAE ALATLYKYTR ARPPSRTLVD PLDAFVASLP SKGLSSAAED ALAAADKTKE LVAKAGRGAY VNQEDLKKRE VPDPGAWGIW RILDGLRGFE A

NT Sequence

atggctcccg ctcagaaaca ccttctcaac actcctcaga cccttgtggt cgattcactt aagggcctgg ccactctcaa tcccaatgtg aaacttgacg aggcccaaag agtgatctac acacctccga ctgaatctaa agtcgctctt ctctcaggcg gcggctccgg tcatgaacct gcccatgccg catttgtggg gcctggactg ttatcggcag caatctgtgg aaacatcttt gcttctccaa acgtcgctca gatccgtcga ggactcgagt tggtgactcg tgaaaagggt ggtcttgtcg tagtcatgaa ctacacgggt gacgctttgc actttggctt ggctgccgag cagcacagga gtgcaggaaa acttggggat gttcgagtct tgatggttgg ggatgatgta gctgttggca gggaacaagg aagcatcgtt ggtagacgag ggctggccgg tacaattctg gtgtacaaag tggctgctgc tctttccgat aaaggtgccg accttgactc tgtagagaac attgccaaat atgtggcttc tcggctaggc actcttggtg taggccttga acactgtcat gtcccaggaa ctcgtgctgg taactcccac cttggagcca acgaagttga agttggcatg ggtattcaca acgaagctgg cacttacaag cttgacatga cgactgcgtc tgagctggtg ggaaaaatgc tcactcaaat caccgatact accgacaaag acaagtcgtt tgtgcctttc aaaggagatg gctctgatga ggtggtgttg ttggtgaacg acttgggagc cataagtgag cttgaaatgg gtggtctcac aaatgaagct gtcaagtggc ttcagtcaag gaagatcaaa gttcgtcgag tccttgctgg cacttacatg acatctctca acatgcctgg tttctccctt actcttcttc tcctccctcc tgcttccgaa aaagcacctt actccgccga tgagatcctt gagtaccttg acactccggc tagtgctcca ggatggaagt ggcatgcggg tagggaaccc ggaacgcttg atgtgaaggc tgaagaggcg tctatcccgg tgcaaaaaga caacgctgtt ttaccctcca ctgatgcaaa aggcttcctt gctgctatcg ctcgggcgtg caaggctctt attgccgccg aacctgaact cactgaacaa gaccagatcg ccggagatgg ggatgcaggt ctcactttgg aggccggcgc caagggtgtg ttgaaggcga ttgaggaggg ccgattgaag ggcgagaatg ttgtgcagga tgtcaaagtt attgccgaaa tcgtcgagga ggacatgggc ggcacttcgg gtgcccttta ctctatattc tttgcgggac tgggtaaatc cttgagagat gctgcgacag agggtgccaa gagcacgtcc cctgaggttt ggagcaaagc tgctgctgaa gccctggcca ctctttacaa atacactcgt gcccgacctc cttctcgaac ccttgttgac ccccttgacg ccttcgttgc ctctttgcct tcaaaaggac tttcatccgc agcggaagac gcccttgcag cagctgataa gaccaaggag ctcgtggcta aggctggtcg aggagcatat gtgaatcagg aggatttgaa gaagcgtgaa gtcccggacc ctggggcttg gggtatctgg cgaattttgg atggcttgcg agggttcgag gcttaa

Details for CrneC.19350.a.B2.GE41000

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMA PAQKHLLNTP QTLVVDSLKG LATLNPNVKL DEAQRVIYTP PTESKVALLS GGGSGHEPAH AAFVGPGLLS AAICGNIFAS PNVAQIRRGL ELVTREKGGL VVVMNYTGDA LHFGLAAEQH RSAGKLGDVR VLMVGDDVAV GREQGSIVGR RGLAGTILVY KVAAALSDKG ADLDSVENIA KYVASRLGTL GVGLEHCHVP GTRAGNSHLG ANEVEVGMGI HNEAGTYKLD MTTASELVGK MLTQITDTTD KDKSFVPFKG DGSDEVVLLV NDLGAISELE MGGLTNEAVK WLQSRKIKVR RVLAGTYMTS LNMPGFSLTL LLLPPASEKA PYSAD

Validated NT Sequence

catcggcgga gtaaggtgct ttttcggaag caggagggag gagaagaaga gtaagggaga aaccaggcat gttgagagat gtcatgtaag tgccagcaag gactcgacga actttgatct tccttgactg aagccacttg acagcttcat ttgtgagacc acccatttca agctcactta tggctcccaa gtcgttcacc aacaacacca cctcatcaga gccatctcct ttgaaaggca cgaacgactt gtctttgtcg gtagtatcgg tgatttgagt gagcattttt cccaccagct cagacgcagt cgtcatgtca agcttgtaag tgccagcttc gttgtgaata cccatgccaa cttcaacttc gttggctcca aggtgggagt taccagcacg agttcctggg acatgacagt gttcaaggcc tacaccaaga gtgcctagcc gagaagccac atatttggca atgttctcta cagagtcaag gtcggcacct ttatcggaaa gagcagcagc cactttgtac accagaattg taccggccag ccctcgtcta ccaacgatgc ttccttgttc cctgccaaca gctacatcat ccccaaccat caagactcga acatccccaa gttttcctgc actcctgtgc tgctcggcag ccaagccaaa gtgcaaagcg tcacccgtgt agttcatgac tacgacaaga ccaccctttt cacgagtcac caactcgagt cctcgacgga tctgagcgac gtttggagaa gcaaagatgt ttccacagat tgctgccgat aacagtccag gccccacaaa tgcggcatgg gcaggttcat gaccggagcc gccgcctgag agaagagcga ctttagattc agtcggaggt gtgtagatca ctctttgggc ctcgtcaagt ttcacattgg gattgagagt ggccaggccc ttaagtgaat cgaccacaag ggtctgagga gtgttgagaa ggtgtttctg agcgggagcc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctc ccgctcagaa acaccttctc aacactcctc agacccttgt ggtcgattca cttaagggcc tggccactct caatcccaat gtgaaacttg acgaggccca aagagtgatc tacacacctc cgactgaatc taaagtcgct cttctctcag gcggcggctc cggtcatgaa cctgcccatg ccgcatttgt ggggcctgga ctgttatcgg cagcaatctg tggaaacatc tttgcttctc caaacgtcgc tcagatccgt cgaggactcg agttggtgac tcgtgaaaag ggtggtcttg tcgtagtcat gaactacacg ggtgacgctt tgcactttgg cttggctgcc gagcagcaca ggagtgcagg aaaacttggg gatgttcgag tcttgatggt tggggatgat gtagctgttg gcagggaaca aggaagcatc gttggtagac gagggctggc cggtacaatt ctggtgtaca aagtggctgc tgctctttcc gataaaggtg ccgaccttga ctctgtagag aacattgcca aatatgtggc ttctcggcta ggcactcttg gtgtaggcct tgaacactgt catgtcccag gaactcgtgc tggtaactcc caccttggag ccaacgaagt tgaagttggc atgggtattc acaacgaagc tggcacttac aagcttgaca tgacgactgc gtctgagctg gtgggaaaaa tgctcactca aatcaccgat actaccgaca aagacaagtc gtttgtgcct ttcaaaggag atggctctga tgaggtggtg ttgttggtga acgacttggg agccataagt gagcttgaaa tgggtggtct cacaaatgaa gctgtcaagt ggcttcagtc aaggaagatc aaagttcgtc gagtccttgc tggcacttac atgacatctc tcaacatgcc tggtttctcc cttactcttc ttctcctccc tcctgcttcc gaaaaagcac cttactccgc cgatgagatc cttgagtacc ttgacacttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for CrneC.19350.a.B3.GE41001

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	23kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS TDAKGFLAAI ARACKALIAA EPELTEQDQI AGDGDAGLTL EAGAKGVLKA IEEGRLKGEN VVQDVKVIAE IVEEDMGGTS GALYSIFFAG LGKSLRDAAT EGAKSTSPEV WSKAAAEALA TLYKYTRARP PSRTLVDPLD AFVASLPSKG LSSAAEDALA AADKTKELVA KAGRGAYVNQ EDLKKREVPD PGAWGIWRIL DGLRGFEA

Validated NT Sequence

atcttactca cttaagcctc gaaccctcgc aagccatcca aaattcgcca gataccccaa gccccagggt ccgggacttc acgcttcttc aaatcctcct gattcacata tgctcctcga ccagccttag ccacgagctc cttggtctta tcagctgctg caagggcgtc ttccgctgcg gatgaaagtc cttttgaagg caaagaggca acgaaggcgt caagggggtc aacaagggtt cgagaaggag gtcgggcacg agtgtatttg taaagagtgg ccagggcttc agcagcagct ttgctccaaa cctcagggga cgtgctcttg gcaccctctg tcgcagcatc tctcaaggat ttacccagtc ccgcaaagaa tatagagtaa agggcacccg aagtgccgcc catgtcctcc tcgacgattt cggcaataac tttgacatcc tgcacaacat tctcgccctt caatcggccc tcctcaatcg ccttcaacac acccttggcg ccggcctcca aagtgagacc tgcatcccca tctccggcga tctggtcttg ttcagtgagt tcaggttcgg cggcaataag agccttgcac gcccgagcga tagcagcaag gaagcctttt gcatcagtgg acatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHST DAKGFLAAIA RACKALIAAE PELTEQDQIA GDGDAGLTLE AGAKGVLKAI EEGRLKGENV VQDVKVIAEI VEEDMGGTSG ALYSIFFAGL GKSLRDAATE GAKSTSPEVW SKAAAEALAT LYKYTRARPP SRTLVDPLDA FVASLPSKGL SSAAEDALAA ADKTKELVAK AGRGAYVNQE DLKKREVPDP GAWGIWRILD GLRGFEA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccactg atgcaaaagg cttccttgct gctatcgctc gggcgtgcaa ggctcttatt gccgccgaac ctgaactcac tgaacaagac cagatcgccg gagatgggga tgcaggtctc actttggagg ccggcgccaa gggtgtgttg aaggcgattg aggagggccg attgaagggc gagaatgttg tgcaggatgt caaagttatt gccgaaatcg tcgaggagga catgggcggc acttcgggtg ccctttactc tatattcttt gcgggactgg gtaaatcctt gagagatgct gcgacagagg gtgccaagag cacgtcccct gaggtttgga gcaaagctgc tgctgaagcc ctggccactc tttacaaata cactcgtgcc cgacctcctt ctcgaaccct tgttgacccc cttgacgcct tcgttgcctc tttgccttca aaaggacttt catccgcagc ggaagacgcc cttgcagcag ctgataagac caaggagctc gtggctaagg ctggtcgagg agcatatgtg aatcaggagg atttgaagaa gcgtgaagtc ccggaccctg gggcttgggg tatctggcga attttggatg gcttgcgagg gttcgaggct tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat