CrneC.19348.a

Two-component system sensor protein

CENTER ID: CrneC.19348.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19348.a.B2.GE40998 histidine kinase domain 918 1198
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_00363
UniProt: J9VEN4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLTPISPRP NSNPRVPPSS AAFLQHLHSD PSSHHITLSG HRVSGLSLSP QSLTEDLLDV NGTSEPPISP SLHPVSSGAS SGPSSLGSIG RAPSKRARPK TAPSRNDEWT PGRSYETSPD LPPLIPPQAD EHLRPNPVGD LCTNAMTIDE GLADAMEVLS TSGEPKSYLI GRSESLAGTA ESHTGPSKPS PASMNWSTFA QAYAHGLFDP NKVPNPPTPN DTPTDFQSAR SSPGQKYSSI SPNHKYSMPT VPHNRSEGSS ENYSSKSSKD SVSTNASSAP STSSASASGK QMSMAASLAA RKKAYELENI RNKPNGLALN TDKLSLPSYS LAAATVRMAS TSILNDLAPL SMPSPERELT DPLASVVSFD SSSTNKDSAR SDPGSSRFPL HHSVSSAANP YTSSLRLPTI QASPVTTPLE GPHRPKGKDK SSPRARHGIV NSRIPSASAP LEKTAEAESS TDYFGAAAIA AVSPQETDSS STATEPTPTA LSLELRKTTP LRSNFVVPES SPRQQKDPLP PFVTPSELNR IYDQYGWLPA PLPPDEAARR RALYRFSILH TAPDINFNRI AHMAKLVFNP KAVLITLIDS DTQWFKSQSG FTVEQGGRIS SFCAHTILPR TDEPFVVLDT SSDWRFENNP NVVGPNSIRF YAGAPLRTAD GFNVGALCIL DDKPRTEFPP RSRLILKEFA AVTMREMELW RDKLQLRVRD KIQTSMEKFT RECLEMDSAS STSDAQTVNK MEHVYSRAAQ LVCSTIGLDG CFILDISQIE MVQTDTPTGK KSIYRANPYG AAENTSPVLE RSESFGPVNP FPVLAAVPTA KSTRALTSYE HEKLSDFMQN HHDGRIFEGA APQWIRYMFP QSFRYGMAVP IYGVDQQPFA MICAYTANPE KQFLEGYELQ FLRAIGVIIL SAVLRRRMVL ADKTKSILIS SVSHELRTPL HGILAAAELL SDTQLDSNQL SFLKTVQTCG NSLIETVNHV LDFTKLSGTQ NCKGSPSDLG KVNLAALVEQ TVEGCWIGQR ARFFMGDSDI GSFYAPPAPT GIVPKSKRAL VGEELSHVET VVDIDMREKG WMVRCEKGGL RRVLMNLVGN SFKFTKNGYI QVSLREMPHE PGSKTIPVEM TVVDTGKGIG KDFLKDQLFH PFSQENPLQT GTGLGLAIVN TIVRSDSVNG KVDVWSAEGV GTEIKVTFDV EVVDDDDGQP SISSNSSVVS ATSTFGHGLS VSFVGFNPKH QGHMLSCEVF TRYVNYWYFS IEDQQRADIL IVNELEGLAN HPVAAQKPII LLSVQRGFES TALAEKINRD GGFCQVVYKP VGPTALQKAL NAAVHWIEEH DSSDKSDILG ANGIGINLAG NSDRPSISRG SSGESHESHS TISELSSARF SQQSNVNSRL PLQRRRSEEN EQTPIRPSMA PRGMTYHSSR KVQRNSNNSA GSTPSQGPPS PTSSIPTIPL ADGGVILKAA TVSAEAPRKQ KMGRVMVVED NVINRRVLGA FLKKRGFEYA EAVDGQAGVE LFENAPPNYW DVILMDISMP IMNGHQATRA IRRIEATRRN SLSDIPIVPP PSKPVTISSQ KAVQARVKIF ALTGLATPDD KREAFWSGVD GYLVKPVSLS SLDIIFKKIG F
NT Sequence
atgtctctca ctccgatatc tcctcggccc aactctaacc ctcgtgtccc cccgtcttcc gctgcattcc ttcagcacct ccacagcgac ccttcttctc atcatatcac cctttcgggc catcgtgtat ctggcctttc tctgtccccg caatctctca ccgaagatct tttggatgtt aatggcactt cagaacctcc tataagtcct tctctccacc cagtatcttc gggcgcgagt tctggaccgt cttctttagg aagtatcggc agagcacctt caaaacgtgc aagacccaaa acagctccat cgagaaatga tgaatggact ccaggaagga gttacgagac aagtcctgat cttccgcccc tcatcccacc acaagcagat gagcatttgc gtccaaaccc ggttggggac ctctgtacca atgccatgac gatagatgag ggattggccg atgcaatgga ggtcctctca acttcaggag agccaaaatc gtatttgata ggtcggtcag agtctctggc cgggacggca gagtcacaca cggggccttc caagccttct cctgcaagta tgaattggtc gacctttgca caagcgtatg cccacggctt gtttgacccc aacaaagtcc ctaatcctcc aacgccgaat gatactccta ccgacttcca gtctgcccga tcatctcctg gccaaaaata ctcttcaatt tcccccaacc acaaatactc aatgcctacc gtcccacata atcgtagcga aggcagctcc gagaattata gttccaaaag ttcaaaggac tcagtatcca cgaacgcgag ttcggccccc tcaacatcaa gcgcatcggc gagtggaaag cagatgtcca tggcggcatc gcttgctgca aggaagaaag catacgagct agaaaatatc cgtaataagc caaatggcct ggcgttaaat accgacaagc tttctcttcc ttcgtattct ctagccgccg ctacagtacg catggcgtca accagtatcc tcaatgatct tgcgcctttg tctatgccta gccctgagcg tgaactcact gacccactag cttctgttgt atctttcgat tcctcaagta caaataagga tagtgcccgc tccgaccctg gatcgtcaag gtttcctttg caccatagcg tttcgagtgc tgcgaaccca tacacatcta gtttgcgatt acctactatt caagcaagcc ccgtgacgac gccccttgaa ggcccacaca ggccgaaagg taaagataag tcttctccgc gagcacgcca cggcatagtt aatagccgca ttccctccgc ttctgctccc ctagagaaga ctgctgaggc agaatcgtca acagattatt ttggagccgc tgcgatcgct gctgtttctc cgcaagagac tgattcttca tccacagcga ctgaacccac tccgacagcc ttatctttgg aattgcggaa gacaacacct ctcaggtcca actttgtggt tcctgaatcg tctccgcggc aacagaaaga ccctttgccg ccctttgtca ccccttcaga actcaatcgt atttacgatc agtatggctg gctgccggct cctttacccc ctgacgaagc agcgagacga agagctctat acagatttag catactgcat actgctccag atatcaactt caatcgcata gcacatatgg ccaaactagt atttaacccc aaagctgttc tcatcacctt gatagacagt gatacacaat ggttcaagtc tcaaagcggt ttcactgtgg agcaaggggg aagaatatct agcttctgcg ctcatacgat tttaccaaga actgatgaac cgtttgtcgt actggataca tcgtcggact ggcgattcga aaataatccc aacgttgtcg gaccgaattc cattcgcttc tatgcaggcg cacctttgcg aacagcggat ggcttcaacg ttggcgcatt gtgtattttg gatgacaagc cacgtacaga gtttccccca cggtcacgtt tgattcttaa ggaatttgct gctgttacta tgagagaaat ggaattatgg cgagataagc ttcaattgcg agttcgagac aagattcaaa caagtatgga aaagttcact cgtgagtgct tggagatgga tagcgcctcg tccacttccg atgcgcaaac ggtcaacaaa atggaacatg tctattcccg cgctgcacaa cttgtctgtt ccaccattgg tttagacggg tgctttattc tggacatctc ccagattgag atggtacaaa cggatactcc gacgggcaag aagtctatct atcgggccaa cccatatggt gcggctgaga acacttcgcc agtgttggaa cgaagcgaat cgtttggacc agtcaacccg tttccagtcc ttgcggctgt ccccacagca aaaagcactc gcgctcttac atcatacgaa cacgaaaagc tttcagactt catgcaaaac catcacgatg gccgaatttt tgaaggtgca gcgcctcaat ggattaggta tatgttccct cagtcgttcc gctacggtat ggccgttccg atctatggtg tagatcaaca gccatttgcg atgatttgtg cgtatactgc taacccggaa aagcaattct tggaaggata cgaattgcag ttcctgcgtg caattggtgt catcattttg tcagcagtgc tcagaaggcg tatggtgcta gcagacaaga cgaaaagcat ccttatctcc tcagtcagtc acgagcttcg tacgcctctg catggtatcc ttgcagctgc ggagcttctc agcgataccc agcttgactc caaccaactc tccttcctca aaactgttca aacatgcggt aactcactta ttgaaacagt caatcatgtc ctcgatttta caaaactgag tggtacccag aactgtaagg gttctccttc ggacttgggc aaagtaaact tggccgcgtt ggtggagcag accgtggagg gatgctggat aggccagcga gcgagatttt tcatgggcga ctcagatatc ggaagcttct atgctccacc tgcaccaact ggtatcgtac ccaaatcaaa gcgggcgctg gtgggagagg aactatcgca tgttgaaacg gttgtggaca ttgacatgcg ggagaagggt tggatggtac gttgcgaaaa gggcggattg cggcgagtct taatgaacct cgtgggaaat tctttcaagt tcaccaagaa tggttacatt caggtttcct tacgtgaaat gcctcacgag ccaggatcga aaacgattcc agtggagatg actgtcgtcg atactggtaa aggtatcggc aaagatttcc tcaaagacca gctgttccat cctttctccc aagaaaatcc gctgcagact ggtacaggcc tcgggctggc aatcgtcaat acgatcgtgc gatcggatag cgtcaacggg aaagttgatg tgtggtctgc cgaaggtgtc ggaaccgaaa tcaaagtcac gtttgacgta gaagtggttg atgacgacga tggacaacca tcaatatcat ccaattcatc tgttgtgtca gctacttcca ccttcgggca tggcttatcg gtttcatttg ttggtttcaa cccaaagcac cagggccaca tgctttcatg cgaggttttc actcgttatg taaattactg gtatttcagc atcgaagatc agcaacgtgc agacatcctc atcgtgaacg agcttgaagg gcttgcaaat catcccgtgg cggctcagaa acctatcatt cttctctctg tacagagagg ttttgaatct actgcccttg ctgaaaaaat taatcgtgac ggaggttttt gtcaagttgt ctacaaacca gtcggtccta ctgcccttca gaaggccctg aatgctgctg ttcactggat tgaggagcat gattcgtcag ataaatcaga tattttaggt gcgaacggta ttggcataaa tcttgcagga aactctgatc gcccttcgat ctcgcgtggc tcttcaggag aaagtcatga atcgcactcc actatcagtg agctctcaag tgctcggttc agtcaacaga gcaacgtcaa ttcgcggctt ccacttcaac gccgcaggtc tgaggagaat gaacaaacac ccatacgacc gagcatggcg ccacgaggga tgacatatca ttcaagtagg aaagtgcagc gtaacagcaa caattcggct ggctcgacac cttcgcaggg gccaccttca cctacatcat caatcccaac cattccttta gcggatggcg gtgtcatcct caaggcagca acggtatcag cagaggcgcc taggaaacag aaaatgggca gggtgatggt cgtcgaagac aatgtaatca atcgcagggt tctaggggcg tttctgaaaa agaggggttt tgaatatgca gaggcggtgg atggacaggc aggcgtggag ctctttgaga acgcacctcc taattattgg gacgtcatcc ttatggatat atcgatgccg atcatgaacg gccatcaagc tacgcgtgct atccgacgta ttgaagcgac acgcaggaac agtctgtctg atatacctat agtgcctccg cccagcaaac cggtgaccat ttcatcccag aaggcggtac aagcacgagt caaaatattc gccttgacag ggttggcgac cccagatgac aagagagagg cattttggag tggcgttgat ggatacttgg tgaaacctgt atcgctgtcg agtttagata ttatctttaa gaagattgga ttttga
Details for CrneC.19348.a.B2.GE40998
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 31kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV LADKTKSILI SSVSHELRTP LHGILAAAEL LSDTQLDSNQ LSFLKTVQTC GNSLIETVNH VLDFTKLSGT QNCKGSPSDL GKVNLAALVE QTVEGCWIGQ RARFFMGDSD IGSFYAPPAP TGIVPKSKRA LVGEELSHVE TVVDIDMREK GWMVRCEKGG LRRVLMNLVG NSFKFTKNGY IQVSLREMPH EPGSKTIPVE MTVVDTGKGI GKDFLKDQLF HPFSQENPLQ TGTGLGLAIV NTIVRSDSVN GKVDVWSAEG VGTEIKVTFD VXXXDDDDG
Validated NT Sequence
atcttactca cttatccatc gtcgtcatcn nnnnnnncta cgtcaaacgt gactttgatt tcggttccga caccttcggc agaccacaca tcaactttcc cgttgacgct atccgatcgc acgatcgtat tgacgattgc cagcccgagg cctgtaccag tctgcagcgg attttcttgg gagaaaggat ggaacagctg gtctttgagg aaatctttgc cgataccttt accagtatcg acgacagtca tctccactgg aatcgttttc gatcctggct cgtgaggcat ttcacgtaag gaaacctgaa tgtaaccatt cttggtgaac ttgaaagaat ttcccacgag gttcattaag actcgccgca atccgccctt ttcgcaacgt accatccaac ccttctcccg catgtcaatg tccacaaccg tttcaacatg cgatagttcc tctcccacca gcgcccgctt tgatttgggt acgataccag ttggtgcagg tggagcatag aagcttccga tatctgagtc gcccatgaaa aatctcgctc gctggcctat ccagcatccc tccacggtct gctccaccaa cgcggccaag tttactttgc ccaagtccga aggagaaccc ttacagttct gggtaccact cagttttgta aaatcgagga catgattgac tgtttcaata agtgagttac cgcatgtttg aacagttttg aggaaggaga gttggttgga gtcaagctgg gtatcgctga gaagctccgc agctgcaagg ataccatgca gaggcgtacg aagctcgtga ctgactgagg agataaggat gcttttcgtc ttgtctgcta gcaccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMV LADKTKSILI SSVSHELRTP LHGILAAAEL LSDTQLDSNQ LSFLKTVQTC GNSLIETVNH VLDFTKLSGT QNCKGSPSDL GKVNLAALVE QTVEGCWIGQ RARFFMGDSD IGSFYAPPAP TGIVPKSKRA LVGEELSHVE TVVDIDMREK GWMVRCEKGG LRRVLMNLVG NSFKFTKNGY IQVSLREMPH EPGSKTIPVE MTVVDTGKGI GKDFLKDQLF HPFSQENPLQ TGTGLGLAIV NTIVRSDSVN GKVDVWSAEG VGTEIKVTFD VEVVDDDDG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc tagcagacaa gacgaaaagc atccttatct cctcagtcag tcacgagctt cgtacgcctc tgcatggtat ccttgcagct gcggagcttc tcagcgatac ccagcttgac tccaaccaac tctccttcct caaaactgtt caaacatgcg gtaactcact tattgaaaca gtcaatcatg tcctcgattt tacaaaactg agtggtaccc agaactgtaa gggttctcct tcggacttgg gcaaagtaaa cttggccgcg ttggtggagc agaccgtgga gggatgctgg ataggccagc gagcgagatt tttcatgggc gactcagata tcggaagctt ctatgctcca cctgcaccaa ctggtatcgt acccaaatca aagcgggcgc tggtgggaga ggaactatcg catgttgaaa cggttgtgga cattgacatg cgggagaagg gttggatggt acgttgcgaa aagggcggat tgcggcgagt cttaatgaac ctcgtgggaa attctttcaa gttcaccaag aatggttaca ttcaggtttc cttacgtgaa atgcctcacg agccaggatc gaaaacgatt ccagtggaga tgactgtcgt cgatactggt aaaggtatcg gcaaagattt cctcaaagac cagctgttcc atcctttctc ccaagaaaat ccgctgcaga ctggtacagg cctcgggctg gcaatcgtca atacgatcgt gcgatcggat agcgtcaacg ggaaagttga tgtgtggtct gccgaaggtg tcggaaccga aatcaaagtc acgtttgacg tagaagtggt tgatgacgac gatggatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t