SSGCID: Target Page

CrneC.19346.a

Cystathionine beta-lyase

CENTER ID:	CrneC.19346.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19346.a.B3.GE40997	kinase domain		477	867

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_02686
UniProt:	J9VKQ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTPSTPGES SLATSVYSLA PKSPVEVHKE RVANWRFSTI CANVDGKDQY GASSTPIYQT ATFKGMDGQY DYTRSGNPTR GALENHLARL YGATQTFALS TGMTCLDTIL RLVRPGETVL AGDDLYGGTN RLLTYLGTHG GVDVRHVDTT DVDKVIPHLG PGNNVKMVLL ESPTNPLLKI ADLQAIADAV HSAAPNALIV VDNTMMSPYL QRPLEIGADI VYDSATKYLS GHHDLMAGII AASRPDICKD IAFIINSVGS GLAPFDSFLL LRGVKTMSLR MDRQMATAQL VALYLDSFGF LVHYPGLKSH PKRDIHYKQA SGAGAVLSFV TGDKALSERI VGGTRLWGIS VSFGAVNSLI SMPCLMSHAS ISAAVRAERG LPENLIRLCV GIEDPRDLVD DLEHSLLQAG AIVPNLQYTP LSQAEAAELY AKDGEAWILE RAKGFKRPST ESITVDKLVS SVKQSLGFST PSKEYKTIDE DIAVSAPGKV ILFGEHAVVH GVTAVASSVD LRCFSVLSPR RDGKIGLEVP NIGVELEWEI SKLPWNLLPV HANGDRHVAD QELDTALLQA VEGAVNTHVE VGKTGIGACV AFLYLYMMIS GEETNALSVT FTASSNLPIS AGLGSSAAYS TCVAASFLLA RQHLTIPSAD RLPKEDTDLV DGWAFLAEKV LHGNPSGIDN AVSVRGGAVA FTRSVGGKQG GMDGLFGFSS VRLLLTNTLI PRDTKTLVAG VSAKRLAEPQ AVNSILDSIQ AISDEARSLL GGGKPVERFI LVKRLEALIK ENHEYLVKLG VSHPSLEMIV AATAQAPFEL ATKLTGAGGG GCALTLIPDA FPESSLNELI QTLEGHGFQP HLTTLGGPGL GVLTIPSKLG QASDQLRDAV RTHDEGEGMV VPKRAGLREA DKEGLHAWAE RLGRWVYA

NT Sequence

atgaccaccc cttcaacacc gggcgaaagc tcgctcgcca cgtctgtcta ttctctcgcc ccaaagtctc cagtggaagt acataaggaa cgagtcgcca actggcgctt ctccaccatc tgcgctaatg tcgatggcaa ggaccagtat ggagctagct caactcccat ctaccagact gccaccttca agggtatgga cgggcaatat gactacacca ggtctgggaa ccccacaaga ggcgctcttg aaaaccatct tgcaagattg tacggagcca ctcaaacatt tgccttgtct actggtatga cttgtcttga cacgatcttg cgacttgtta ggccagggga aaccgttctg gccggcgacg acttgtacgg aggtaccaac cgacttttga catacctcgg gacgcatggc ggtgtcgatg tcaggcatgt tgatactaca gatgtggaca aggtaatccc tcaccttgga ccaggaaaca atgtcaagat ggttctttta gaatccccta cgaaccctct gttgaaaatt gccgacctgc aagcaattgc cgacgctgtt cactctgctg cccctaacgc tttgattgtg gttgacaaca ctatgatgtc tccttatctg caacggccac ttgagattgg tgccgatatt gtttacgact ccgctaccaa atatctctca ggtcatcacg atcttatggc tggtatcatt gcagcttctc gacccgatat ctgcaaagac attgcattca ttattaactc tgttggatcc ggccttgctc ctttcgactc tttccttttg ctccgtggcg tcaagaccat gtctctcagg atggacaggc agatggccac tgctcaactt gtggctcttt accttgattc atttggcttc ttggtccact accctggtct caagagccat cctaagaggg atatccacta caaacaggcc tctggtgctg gtgcggtgtt aagcttcgtt actggcgaca aggctcttag cgaacggatc gtcgggggga caaggctgtg gggtatcagt gtgtctttcg gagctgtcaa cagtttgatc agtatgccat gtttaatgtc tcacgcctcc atttccgctg cagttcgtgc tgaacgaggg ctcccagaaa atcttattcg actttgcgtt ggtatcgaag accctagaga tcttgttgat gacttggagc actcgcttct ccaagcagga gctatcgttc ccaatctaca atatacccct ctctcccagg ctgaggcggc agagttgtac gctaaggacg gtgaagcgtg gatcctggaa cgtgccaagg gtttcaagcg gccttctact gagtccatta ctgttgacaa gcttgtcagc agcgtgaagc aaagtctcgg tttctccacc ccttccaaag agtacaagac gatcgatgaa gacatcgcgg tttctgcccc aggcaaggtt attcttttcg gtgagcacgc tgtcgtccac ggcgtcactg ccgtcgcgtc cagcgtcgac ctccgatgct tttctgtctt gtctcctcgt cgtgacggaa agattggatt ggaagtaccc aatattggtg tggagctcga gtgggaaatc agcaagcttc cttggaattt gcttcccgtg cacgcaaatg gcgataggca cgtcgccgac caggagcttg acacggcgtt gcttcaagct gtggagggtg ctgtcaacac tcatgttgaa gtgggtaaga cgggcatagg tgcctgtgtg gccttcttat atctctacat gatgatctca ggtgaagaaa ccaatgctct ttctgtaacc tttactgctt cttcaaacct ccctatctct gctggtctcg gatcgtcagc cgcatactcc acttgtgtcg cggcgtcttt ccttctcgct cgtcagcacc tcaccattcc ttcggccgat cgccttccca aggaagacac tgacttggtc gatggatggg cctttttggc cgaaaaggtt ttgcacggaa accctagtgg tatcgataac gccgtctctg tcagaggtgg ggctgttgct ttcaccagat ctgttggcgg caagcagggc ggtatggatg gtctttttgg gttttcctct gtccgattac tcttgaccaa tactcttatc cccagagaca ccaagacttt ggttgccggt gtttccgcaa agcggctcgc tgaacctcag gccgttaact cgattctcga ttctatccaa gccatttctg atgaagcacg ttccttgctc ggcggcggca agcctgtgga acgctttatc ctcgtcaagc gtcttgaggc tctcatcaag gagaaccatg aatacttagt gaagctcgga gtgtcgcatc cttctttgga aatgatcgtg gcagctaccg cccaggcacc tttcgagtta gccaccaagt tgaccggtgc tggtggcggc ggatgtgctc ttactcttat tccggatgct ttccctgaat cctccctcaa cgagctcatc caaaccctcg aaggccatgg cttccaaccg cacctcacca cattgggcgg ccctggtctc ggtgtcctca cgatcccctc caagcttggt caagcctctg accaacttcg agatgctgtg aggacgcatg atgagggtga aggcatggtt gtgccgaaga gagctggtct tagagaagct gataaggaag gtttgcacgc atgggcggaa aggttgggta gatgggttta tgcttaa

Details for CrneC.19346.a.B3.GE40997

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	42kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	None
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	91

Validated AA Sequence

MAHHHHHHMT IDEDIAVSAP GKVILFGEHA VVHGVTAVAS SVDLRCFSVL SPRRDGKIGL EVPNIGVELE WEISKLPWNL LPVHANGDRH VADQELDTAL LQAVEGAVNT HVEVGKTGIG ACVAFLYLYM MISGEETNAL SVTFTASSNL PISAGLGSSA AYSTCVAASF LLARQHLTIP SADRLPKEDT DLVDGWAFLA EKVLHGNPSG IDNAVSVRGG AVAFTRSVGG KQGGMDGLFG FSSVRLLLTN TLIPRDTKTL VAGVSAKRLA EPQAVNSILD SIQAISDEAR SLLGGGKPVE RFILVKRLEA LIKENHEYLV KLGVSHPSLE MIVAATAQAP FELATKLTGA GGGGCALTLS GCFP

Validated NT Sequence

gaccagggcc gcccaatgtg gtgaggtgcg gttggaagcc atggccttcg agggtttgga tgagctcgtt gagggaggat tcagggaaag catccggaaa gagtaagagc acatccgccg ccaccagcac cggtcaactt ggtggctaac tcgaaaggtg cctgggcggt agctgccacg atcatttcca aagaaggatg cgacactccg agcttcacta agtattcatg gttctccttg atgagagcct caagacgctt gacgaggata aagcgttcca caggcttgcc gccgccgagc aaggaacgtg cttcatcaga aatggcttgg atagaatcga gaatcgagtt aacggcctga ggttcagcga gccgctttgc ggaaacaccg gcaaccaaag tcttggtgtc tctggggata agagtattgg tcaagagtaa tcggacagag gaaaacccaa aaagaccatc cataccgccc tgcttgccgc caacagatct ggtgaaagca acagccccac ctctgacaga gacggcgtta tcgataccac tagggtttcc gtgcaaaacc ttttcggcca aaaaggccca tccatcgacc aagtcagtgt cttccttggg aaggcgatcg gccgaaggaa tggtgaggtg ctgacgagcg agaaggaaag acgccgcgac acaagtggag tatgcggctg acgatccgag accagcagag atagggaggt ttgaagaagc agtaaaggtt acagaaagag cattggtttc ttcacctgag atcatcatgt agagatataa gaaggccaca caggcaccta tgcccgtctt acccacttca acatgagtgt tgacagcacc ctccacagct tgaagcaacg ccgtgtcaag ctcctggtcg gcgacgtgcc tatcgccatt tgcgtgcacg ggaagcaaat tccaaggaag cttgctgatt tcccactcga gctccacacc aatattgggt acttccaatc caatctttcc gtcacgacga ggagacaaga cagaaaagca tcggaggtcg acgctggacg cgacggcagt gacgccgtgg acgacagcgt gctcaccgaa aagaataacc ttgcctgggg cagaaaccgc gatgtcttca tcgatcgtca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHTI DEDIAVSAPG KVILFGEHAV VHGVTAVASS VDLRCFSVLS PRRDGKIGLE VPNIGVELEW EISKLPWNLL PVHANGDRHV ADQELDTALL QAVEGAVNTH VEVGKTGIGA CVAFLYLYMM ISGEETNALS VTFTASSNLP ISAGLGSSAA YSTCVAASFL LARQHLTIPS ADRLPKEDTD LVDGWAFLAE KVLHGNPSGI DNAVSVRGGA VAFTRSVGGK QGGMDGLFGF SSVRLLLTNT LIPRDTKTLV AGVSAKRLAE PQAVNSILDS IQAISDEARS LLGGGKPVER FILVKRLEAL IKENHEYLVK LGVSHPSLEM IVAATAQAPF ELATKLTGAG GGGCALTLIP DAFPESSLNE LIQTLEGHGF QPHLTTLGGP GLGVLTIPS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacgatcg atgaagacat cgcggtttct gccccaggca aggttattct tttcggtgag cacgctgtcg tccacggcgt cactgccgtc gcgtccagcg tcgacctccg atgcttttct gtcttgtctc ctcgtcgtga cggaaagatt ggattggaag tacccaatat tggtgtggag ctcgagtggg aaatcagcaa gcttccttgg aatttgcttc ccgtgcacgc aaatggcgat aggcacgtcg ccgaccagga gcttgacacg gcgttgcttc aagctgtgga gggtgctgtc aacactcatg ttgaagtggg taagacgggc ataggtgcct gtgtggcctt cttatatctc tacatgatga tctcaggtga agaaaccaat gctctttctg taacctttac tgcttcttca aacctcccta tctctgctgg tctcggatcg tcagccgcat actccacttg tgtcgcggcg tctttccttc tcgctcgtca gcacctcacc attccttcgg ccgatcgcct tcccaaggaa gacactgact tggtcgatgg atgggccttt ttggccgaaa aggttttgca cggaaaccct agtggtatcg ataacgccgt ctctgtcaga ggtggggctg ttgctttcac cagatctgtt ggcggcaagc agggcggtat ggatggtctt tttgggtttt cctctgtccg attactcttg accaatactc ttatccccag agacaccaag actttggttg ccggtgtttc cgcaaagcgg ctcgctgaac ctcaggccgt taactcgatt ctcgattcta tccaagccat ttctgatgaa gcacgttcct tgctcggcgg cggcaagcct gtggaacgct ttatcctcgt caagcgtctt gaggctctca tcaaggagaa ccatgaatac ttagtgaagc tcggagtgtc gcatccttct ttggaaatga tcgtggcagc taccgcccag gcacctttcg agttagccac caagttgacc ggtgctggtg gcggcggatg tgctcttact cttattccgg atgctttccc tgaatcctcc ctcaacgagc tcatccaaac cctcgaaggc catggcttcc aaccgcacct caccacattg ggcggccctg gtctcggtgt cctcacgatc ccctcctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t