CrneC.19345.a

Serine/threonine-protein kinase ATR

CENTER ID: CrneC.19345.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19345.a.B3.GE40995 kinase domain 1745 2182
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_02233
UniProt: J9VV52

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQLATQYNI QGADLQSLLP RLLSEGLTPS GDQQQSSPGD LVQVLLEHCI LKPLSMSITV NVQQATYTLA IIKRQATLSP RFLTETYNQA PFYQWLIPRL LQIACSSIAS VPTDESVQVV VYIIQILGRD QPDDEDTWAK GPGRAAQILS QLTLFCQDVI DGHESKLYGF AELPSNICAV LSIVSAILQL DLPFALSFIS TACAQLSEAS RLIDTPESQL IYIQTVNKAL GRNIHQGLTK ACSYVSSIDI VDGADRKIAL MTFYEKMASV HEGLKYDVWW SLLRQCGNLD IKDDSIGFPG ICHLLTPVGP RLSRDTIKQI VGLDQIKEWE KTAKEISIQT GNSVKLDTVS ALLQKDVQKS KKRKRRTSGN LIADLRELLP DLPDPPEGSS IVDILIQNSL SLSAIAHLMP QIVRFAVGEH DWPRYPTSKE FNPKVLELWI KVNVFDLSMM NALSSIIEHA PSGAIAAESQ NLEIIYEKVV MGLNARERPM RVAAGRTLSL FFMTQNAGVD HIIARKNQQR YIEAASTPLQ SPATAETAVL LLGDLGRLAQ GESLCFVLQL LLRQLGSYNS PLRSLAYTEL VALAKHHHKT PYTLLSPFLD RISVLLADNI IRSPYMISET MQFIGSTRQN FLHTTLPHIL PALVLSRNRE ALIHVASIVK QRLGRLFMDH IAGILAQVFL HPQQTAESLD FLVDLIRSMT HGYSEHEPLI TVESLMGSCM VDLMVILVVE LGDQDKAARR AAKLGLSKAM AYQRTGTDLG AFLKPYMLGV ISQLNDMLHD VLGKKSVEYK KKIIRSMGVL IKLVGDSMSS FSPQIMASLQ STLGIKELRH ETLNTWAVFT STLKYADIGP FVGRTTGALV ANWPTFDKTA KSVAIRIIDE IADSANDLSQ FVEEVVGMDH IDELQRAASL LTAQRKKWPI DIRITKVLDR VASKNIAISL ASIRELRLLL VTLQESIQDL VKGDTFNPVA ARLMSTLLSI ATRDGDCQEL RDLSYECLGI IGALDPDRLG YYVESNTFTI TSNFADHKES LDFALHLVRD LLVDAFRATN DTKHQNHLAF AIQELLRFCG FSLKVIHPAS KIDPSIRQRW QSLPKDQLET LTPLLESRFT LHDVSFRTFS HPIYVAAPTY REWLQRWATD LISKVMSMPD TDRSVSDSKA IFGVFCGVLR NQDVSVAHHI LPHLVLNVLL SGVREYRDEI CLEIKTVLQD QVQPTSPADR RSLSAQVIFD LMDHLSKWLR LQRVKGSHLD RGERSKVVEG VLSSIETELM AHAALQSKAY ARSLRSFEER IIQLREEKKD TAELQTYFER LHQIYAELDE PDGMEGVSAF VISPSLEHQI REHESTGRWT SAQSCWEVRL QQSPDDPTLH VGLLKCLRNL GHYDTLRTHI RGVITRHPDW SLQLAPFAAE AAWIIGDWDT VRQVGPDCPP IGQALLALHE DGDLSSVLTR VRREVGAGIT GKGYTPVYEA LLQLHLVQEI AMIQDTKKEI QIVSKSKNRH KVVQQHVRQL TASLDSRFYT TSPAFRVREA ILSIRRTALG LMNTPSLNPE IGDAWILSSK IARKAGYEQT AYSATLQARE ADAPFAFVQE AKLRRAQGSV FKALTDLQNT LAPLATDSKA SENIEQDSFR RSRDLAKAVL LLARWANETD RFDQNEIVKR YTQAITLCDT LESPYYHLGH FYDGQVGDPA QKIIYNYHTC NYYSIALRHG VKYIFQTMPR MLTLWLDLGD TKDAKKKKFI SKIHSVVGEA AHDLPAYQFY TAFPQIVSRI VHPSVDVSRI LRSIMVRVIS EYPQQALWPM VGVMKSCQDE RRNACLAVFQ KATSISTIIR DADTFSSTLL KFTDHKVDGR KREKSIQSHF PYVKSAFPTK MILPLQDALT CNLPTSSDTV KTHNPFPNAP IEIHDVEDRV DVMPSLQRPK KLVFIGSDGK AYPFLCKPHD DLRKDARVMD LNSMINKLLK SASESRRRQL YVRTYAVMPL NEECGLLEWV TNTHGFKGIL ETNYGRQNKK IFINEVIDLL TTTRKQCPPE VLTAVFKDKV LPLYKPTVFR EWFLTSWPEP SAWLSSRLAY SRTLAVMSMI GYILGLGDRH GENILFDGLS GDTVHVDLNC LFEKGKTLEI PERVPFRLTQ NMVDALGVTG VEGVFRKAAE ITMSILRSNS DSLMSVLEAF VHDPLVEWTS RGRGKSDPRD IRSNADKNLH PIKRKLRGVM NEGTVVSVPN QVETLIKEAT SPRNLGAMYV GWAPWL
NT Sequence
atgacgcagc tcgccacaca gtacaacata cagggagctg atctccaatc cctccttcct cgcctcctca gtgaaggtct cactccttca ggagatcaac agcaatcgag tccaggcgat ctcgtccaag tcctgctgga gcactgcatc ctcaaacctc tgtcaatgag tatcacagta aacgttcaac aagccacata tactcttgcc atcatcaagc gacaagcaac actctctcct agatttctga cggaaacata caatcaggca cccttttacc aatggctgat ccctaggctg ctgcaaatag cttgctcatc aatcgcgtct gttcctacag acgaatcggt acaggtggtt gtatatatca ttcagatatt aggacgagac cagcccgacg atgaggatac atgggctaaa ggaccgggaa gggcagcgca gatactgagc cagttgactt tgttttgtca agacgtgata gatgggcacg aatcaaagct ttatggcttt gccgagcttc caagcaacat ttgtgccgtg ttatcaattg tatctgccat tcttcagctc gatctccctt ttgcgttatc atttatctca acagcttgcg cccagctttc tgaagctagc cgtttaatag atacaccaga atcgcaatta atatacatac agacagtgaa caaagctctt gggcggaata tccatcaagg attgaccaag gcttgctcgt atgtctcaag tattgatatt gtcgatggtg cggacagaaa aatagcacta atgactttct atgagaaaat ggcctcagtc cacgagggat tgaaatatga tgtgtggtgg tcacttttac gccaatgcgg gaatctagat attaaggacg attctattgg cttccctgga atctgccatc ttttgactcc tgtaggccct cgcctttccc gcgatactat caaacaaatc gtcggccttg accaaatcaa ggagtgggaa aaaacagcca aagaaatctc catacaaaca ggcaactcgg tcaagcttga cactgtttct gctcttttgc agaaggatgt tcagaaatcc aagaagagga aaaggaggac aagcgggaat ttgattgcag accttcgtga acttctccca gatcttcccg accctcccga aggaagctct atagtcgata tacttattca gaattcttta tccttgtctg ccatcgcgca tctcatgcct caaattgttc gcttcgccgt tggcgagcat gattggcccc gatatcctac atccaaagag tttaatccta aggtcctgga gttatggatc aaagtcaatg tgttcgactt atcgatgatg aatgcactga gcagcataat cgagcatgct ccatctggtg cgatagccgc agaatcccaa aacttggaaa ttatctatga aaaagtcgtc atgggtttaa atgcacgcga gcgtcccatg agagtagctg ccggtcgaac gttatcactt tttttcatga cccaaaatgc tggtgtggat cacatcattg ccaggaaaaa tcaacaaagg tacattgagg cggcctctac cccacttcag tctccagcaa ctgccgaaac tgccgtgctt ttgctcggtg acctcggcag gcttgcccag ggggagtcat tatgcttcgt cctgcagtta cttctacgcc agctcggatc atacaattct cctcttagat cccttgctta cacagagctc gtcgccctgg ctaagcatca tcataaaaca ccgtatactc ttctgtctcc gttcctcgat cgcatcagtg tactcctggc cgacaatatc attcgctctc cttatatgat ctccgagaca atgcagttca tcggttctac tcgacaaaac ttcttgcata cgacattgcc gcatatctta ccagcgcttg tactgtcgcg caaccgagaa gccctaatac atgtggcgag tatcgtaaag cagcgattag gtcgtttatt tatggaccat attgctggta tcctcgcgca agtctttctc cacccccagc agacggctga aagtcttgac ttcctggttg acctcattcg aagcatgact catggttatt ctgagcatga gcctttgatc actgtagaat ctctcatggg atcttgtatg gttgacctta tggtgattct cgttgttgaa ctaggtgacc aagacaaggc cgccaggaga gctgcaaagt tagggttgag caaggccatg gcatatcaac ggacaggtac cgacttagga gcatttctta agccctatat gctaggagtc atttctcaac tcaacgatat gctacatgac gtgctaggca aaaagtcagt agaatacaaa aagaaaatca tcaggagcat gggcgtctta atcaaactag tgggggattc gatgtcttct ttttcaccac agatcatggc gagcttacaa agcacacttg gcatcaaaga gctccgtcat gaaactctta acacatgggc agtcttcact tctactctca aatacgccga tattggtcct tttgtaggcc gcactactgg tgcactagtg gcaaattggc ccacatttga caagaccgcg aaatctgtcg ccatccgaat catcgatgag atcgccgaca gtgcaaatga tctgagtcaa ttcgtcgaag aagttgtagg catggatcat atcgacgaac tccagagagc tgccagtcta ttgacagccc agcggaagaa atggccgatt gatattcgta tcacaaaagt tcttgatagg gttgctagca agaacatagc aatttcgttg gcttctatcc gtgagttgag gctactgctg gtcactcttc aagaaagcat ccaggatctt gtgaaaggag atacttttaa tccagtggcc gctcgactta tgtctaccct tttatccatt gctactagag atggagattg tcaggagttg agggatctca gctatgaatg tttgggtatt atcggcgcct tggatcctga ccgtctggga tactacgtcg aaagcaatac atttactatc acctcgaact tcgctgatca caaggagtca ctcgattttg cccttcatct tgtgcgtgat cttctcgtgg atgcttttcg cgccaccaac gatacaaagc atcagaacca tcttgctttt gctatacagg agctccttcg tttttgtggg ttttccctca aagttatcca cccagcaagt aaaatcgatc cttcaattcg acaacgatgg caaagcctcc caaaagatca gctcgaaacc ctgacaccac tgctcgaatc acgttttacc ctgcatgatg tttcttttcg caccttctcg caccccatat atgtagccgc cccaacatat cgcgaatggc tacagcgctg ggcgacagac cttattagca aagtgatgtc catgcccgat actgatcgct ctgtcagcga tagcaaagct atctttggtg ttttttgtgg cgtactgaga aatcaagatg tcagtgtagc ccaccatatc cttcctcatc tcgttctcaa tgttctactt tctggcgtac gcgagtaccg cgacgagatt tgccttgaaa ttaaaactgt tttgcaagat caagtacagc ctactagtcc ggccgacaga cgatccctca gtgcacaggt tatctttgac ctgatggacc acctgagtaa atggttgcga ttacagcgag tgaagggtag ccacctagat cgaggagaaa gatccaaagt cgttgagggg gtactgtcta gtatagaaac cgagctcatg gcgcatgctg ctttgcagag caaggcatat gcgaggtctc tgagaagttt tgaggaaagg attatccaat taagggagga gaaaaaagat actgcggaac tgcaaacata ttttgagcga ctgcaccaaa tttatgcgga actggacgaa ccagatggga tggagggtgt ttcagcattt gttatttcgc cttctttaga gcatcaaatc agggaacatg agagcacagg tagatggacg tcggcgcaaa gctgctggga ggtaaggttg cagcaatctc cagatgaccc cacgttacac gttggacttc tgaaatgtct tcgaaatctt ggacattatg acacccttcg aactcatatc cggggagtca ttactcgcca tccagattgg tcacttcagc tagccccgtt tgctgcagag gccgcatgga tcatcggtga ttgggacaca gtccgccagg ttggtccgga ctgcccgccc atcggccaag ctttgcttgc tctacatgaa gacggtgacc tttcatctgt tctcactcga gtgcgtcgtg aagtaggggc tggcatcact ggaaagggtt atacacccgt atatgaggca ttgctccagc tccaccttgt gcaagagatt gctatgattc aagacacgaa gaaagaaatt caaattgtca gcaagagcaa gaaccgccat aaagtcgttc agcaacatgt acgccagctc actgcctccc ttgattcgag attctatacg acatctcctg cttttcgagt acgggaagct attctcagca tacgtcgcac agcattgggt ctcatgaaca caccctcact taaccctgag ataggcgacg cttggattct tagctcgaaa atagctagga aggctggcta tgagcaaacg gcatatagcg ccactctaca agccagagag gcagatgcac cattcgcatt cgtacaagag gcaaaacttc gtcgtgctca aggcagtgtt ttcaaagcat taacagacct tcagaatact ttggcgcctt tggctactga tagcaaggcc agtgaaaata tcgagcaaga ctcatttcga aggagcaggg atctggcaaa ggctgtactt ctgcttgcaa gatgggcgaa cgaaacagac agatttgatc agaatgaaat agtcaaacgc tatacacaag ctatcactct atgtgatact ctagagtctc cttactatca tttgggtcat ttttacgatg gtcaggttgg tgatcctgca caaaaaataa tctacaatta ccatacttgc aattactaca gcatagctct gcgccacggc gtgaaataca tcttccagac gatgcccagg atgctcactc tatggttaga tctgggagac accaaagacg ccaaaaagaa gaaatttatt tccaaaatcc attctgtggt cggcgaagct gctcatgatc taccagcata tcagttctac actgctttcc ctcaaatcgt ctctcggatt gtccatccta gcgtagatgt ttcaaggatt ctgcgttcaa tcatggtccg tgtcatctct gaatatcctc aacaggctct atggccaatg gtcggtgtca tgaaatcttg tcaagacgaa aggcgaaacg cctgtttagc agtctttcaa aaagcaacct ctatctcgac gataatcaga gatgcagata ccttttcaag cactctgtta aaatttactg accacaaggt agatggtcgc aagcgggaga aatcaatcca gtcccatttt ccctatgtta aatccgcatt ccctaccaag atgatattgc ctcttcaaga cgctttgacg tgtaatctgc ccacgtcatc tgacactgtc aagactcaca atcctttccc caacgcacca atagaaattc atgatgtcga ggatcgcgtg gacgtcatgc catcacttca aagaccaaag aaacttgtct tcataggcag cgatggcaaa gcatatcctt ttctttgcaa gccgcatgac gatttgagaa aggatgctcg cgtcatggat ctcaattcca tgattaacaa actcctgaaa agtgcctcgg agtctagaag acgtcaactt tacgttcgga cctatgctgt catgccgctg aatgaagagt gtggtttgct ggagtgggtg actaataccc acggattcaa gggcatcctg gaaaccaatt acggtcgcca gaataagaag atttttatca acgaagtgat tgacctactc actacaacga ggaaacaatg cccgccagaa gtcctcacag ccgtcttcaa ggataaagtg ttgccacttt ataaaccgac agtctttcgc gagtggtttt tgacatcctg gccagaacct tcagcatggt tatccagtcg cttagcctat agtcgcacac tggccgtcat gtcgatgatc ggttacattc taggcctcgg tgatcgacat ggtgaaaata tcttattcga tggactctca ggtgatactg tacatgtcga tctcaattgt ttgttcgaaa agggcaaaac ccttgagatc cccgaacgtg tacctttccg actcacacaa aacatggtcg acgccttggg tgtaactggt gtcgaaggcg tatttcgcaa agctgcagag ataactatga gtatcctacg ttcaaatagc gactctttaa tgagtgtctt ggaggctttt gtccacgacc ctctcgttga atggaccagt agaggtcgcg gaaagtcaga tcccagagac attcgaagta atgccgacaa aaacctccat cctatcaaac gcaaacttcg aggagtgatg aatgaaggca cagtcgtttc cgtgcccaac caggttgaaa ctttgattaa ggaagccact tctcctagaa atcttggtgc aatgtacgtt ggatgggccc catggttgta a
Details for CrneC.19345.a.B3.GE40995
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 51kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS VVGEAAHDLP AYQFYTAFPQ IVSRIVHPSV DVSRILRSIM VRVISEYPQQ ALWPMVGVMK SCQDERRNAC LAVFQKATSI STIIRDADTF SSTLLKFTDH KVDGRKREKS IQSHFPYVKS AFPTKMILPL QDALTCNLPT SSDTVKTHNP FPNAPIEIHD VEDRVDVMPS LQRPKKLVFI GSDGKAYPFL CKPHDDLRKD ARVMDLNSMI NKLLKSASES RRRQLYVRTY AVMPLNEECG LLEWVTNTHG FKGILETNYG RQNKKIFINE VIDLLTTTRK QCPPEVLTAV FKDKVLPLYK PTVFREWFLT SWPEPSAWLS SRLAYSRTLA VMSMIGYILG LGDRHGENIL FDGLSGDTVH VDLNCLFEKG KTLEIPERVP FRLTQNMVDA LGVTGVEGVF RKAAEITMSI LRSNSDSLMS VLEAFVHDP
Validated NT Sequence
agagggtcgt ggacaaaagc ctccaagaca ctcattaaag agtcgctatt tgaacgtagg atactcatag ttatctctgc agctttgcga aatacgcctt cgacaccagt tacacccaag gcgtcgacca tgttttgtgt gagtcggaaa ggtacacgtt cggggatctc aagggttttg cccttttcga acaaacaatt gagatcgaca tgtacagtat cacctgagag tccatcgaat aagatatttt caccatgtcg atcaccgagg cctagaatgt aaccgatcat cgacatgacg gccagtgtgc gactataggc taagcgactg gataaccatg ctgaaggttc tggccaggat gtcaaaaacc actcgcgaaa gactgtcggt ttataaagtg gcaacacttt atccttgaag acggctgtga ggacttctgg cgggcattgt ttcctcgttg tagtgagtag gtcaatcact tcgttgataa aaatcttctt attctggcga ccgtaattgg tttccaggat gcccttgaat ccgtgggtat tagtcaccca ctccagcaaa ccacactctt cattcagcgg catgacagca taggtccgaa cgtaaagttg acgtcttcta gactccgagg cacttttcag gagtttgtta atcatggaat tgagatccat gacgcgagca tcctttctca aatcgtcatg cggcttgcaa agaaaaggat atgctttgcc atcgctgcct atgaagacaa gtttctttgg tctttgaagt gatggcatga cgtccacgcg atcctcgaca tcatgaattt ctattggtgc gttggggaaa ggattgtgag tcttgacagt gtcagatgac gtgggcagat tacacgtcaa agcgtcttga agaggcaata tcatcttggt agggaatgcg gatttaacat agggaaaatg ggactggatt gatttctccc gcttgcgacc atctaccttg tggtcagtaa attttaacag agtgcttgaa aaggtatctg catctctgat tatcgtcgag atagaggttg ctttttgaaa gactgctaaa caggcgtttc gcctttcgtc ttgacaagat ttcatgacac cgaccattgg ccatagagcc tgttgaggat attcagagat gacacggacc atgattgaac gcagaatcct tgaaacatct acgctaggat ggacaatccg agagacgatt tgagggaaag cagtgtagaa ctgatatgct ggtagatcat gagcagcttc gccgaccaca gacatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHSV VGEAAHDLPA YQFYTAFPQI VSRIVHPSVD VSRILRSIMV RVISEYPQQA LWPMVGVMKS CQDERRNACL AVFQKATSIS TIIRDADTFS STLLKFTDHK VDGRKREKSI QSHFPYVKSA FPTKMILPLQ DALTCNLPTS SDTVKTHNPF PNAPIEIHDV EDRVDVMPSL QRPKKLVFIG SDGKAYPFLC KPHDDLRKDA RVMDLNSMIN KLLKSASESR RRQLYVRTYA VMPLNEECGL LEWVTNTHGF KGILETNYGR QNKKIFINEV IDLLTTTRKQ CPPEVLTAVF KDKVLPLYKP TVFREWFLTS WPEPSAWLSS RLAYSRTLAV MSMIGYILGL GDRHGENILF DGLSGDTVHV DLNCLFEKGK TLEIPERVPF RLTQNMVDAL GVTGVEGVFR KAAEITMSIL RSNSDSLMSV LEAFVHDPLV EWTSRG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctgtgg tcggcgaagc tgctcatgat ctaccagcat atcagttcta cactgctttc cctcaaatcg tctctcggat tgtccatcct agcgtagatg tttcaaggat tctgcgttca atcatggtcc gtgtcatctc tgaatatcct caacaggctc tatggccaat ggtcggtgtc atgaaatctt gtcaagacga aaggcgaaac gcctgtttag cagtctttca aaaagcaacc tctatctcga cgataatcag agatgcagat accttttcaa gcactctgtt aaaatttact gaccacaagg tagatggtcg caagcgggag aaatcaatcc agtcccattt tccctatgtt aaatccgcat tccctaccaa gatgatattg cctcttcaag acgctttgac gtgtaatctg cccacgtcat ctgacactgt caagactcac aatcctttcc ccaacgcacc aatagaaatt catgatgtcg aggatcgcgt ggacgtcatg ccatcacttc aaagaccaaa gaaacttgtc ttcataggca gcgatggcaa agcatatcct tttctttgca agccgcatga cgatttgaga aaggatgctc gcgtcatgga tctcaattcc atgattaaca aactcctgaa aagtgcctcg gagtctagaa gacgtcaact ttacgttcgg acctatgctg tcatgccgct gaatgaagag tgtggtttgc tggagtgggt gactaatacc cacggattca agggcatcct ggaaaccaat tacggtcgcc agaataagaa gatttttatc aacgaagtga ttgacctact cactacaacg aggaaacaat gcccgccaga agtcctcaca gccgtcttca aggataaagt gttgccactt tataaaccga cagtctttcg cgagtggttt ttgacatcct ggccagaacc ttcagcatgg ttatccagtc gcttagccta tagtcgcaca ctggccgtca tgtcgatgat cggttacatt ctaggcctcg gtgatcgaca tggtgaaaat atcttattcg atggactctc aggtgatact gtacatgtcg atctcaattg tttgttcgaa aagggcaaaa cccttgagat ccccgaacgt gtacctttcc gactcacaca aaacatggtc gacgccttgg gtgtaactgg tgtcgaaggc gtatttcgca aagctgcaga gataactatg agtatcctac gttcaaatag cgactcttta atgagtgtct tggaggcttt tgtccacgac cctctcgttg aatggaccag tagaggttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at