CrneC.19343.a

Atypical/ABC1/ABC1-C protein kinase

CENTER ID: CrneC.19343.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19343.a.B2.GE40992 kinase domain 833 998
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_07359
UniProt: J9VIZ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIPIRHKSCS LASLAGRTLP RATCRAPQQY RFSSSPATPP ADQEEHQIKP LTGSAKLFAD AGAEEKITSN ASNRDHLRMT QGPVWTGDES TTDAVLRMLV DAHKPLRDKG GVKHNAADEK IKGWMKGLQL EPRLSPGPSS PPEEAQEIAL ESVEKENPHR TKIPPHLHRP WHATYTGESR TAEAPKVKYG AFINKRTDGD SLTNLLELQL PPNADGKTRA KVKAARKSSM LVGRMDKARE GALDYKLGLG VDDTYMVETG EEGEEGQTFK GNRQIKGSSV LGAQRGSASG MKAWAGLVED RILRAREAGF FKNASGKGKP IVADPETRNP HIERGELFMN RIIKRQGALP PWIELQNLLD SNLRSFRATL LTTYKTQLVR NVISTNALHP LPPLHTIPDR DEAWEAREFK FHQENVKQIN DLVRRMNTQA PSPARRHLIT LEGELNKIRG NVLKNEVWEE IKRRAEESIN MPYQQERPGL APFIFDGENW TKLKNATKRS FGTVAVSDTA PLANASLHGL EGSSKGYSGG PGGDHSSGPS SYSGRDPRPL RLMVMAGVSV GVIIYLRRRP VKNDSAHLDS FRHHLQPTPP PQSDIVASSP SEPPLTFIRI IEIYILEPIG TFFRFLHLAC LFVPVILSSP MLLVGKPGKR HRSRSGRPIN EEEQTWGAIW WYGFLVKQME RAGPSFIKLG QWAASRADLF PAELCEKMSK LHSNGRPHSL GYTKKVMEAA FNMGFDDIFE EFGEEPIGCG AIAQVYKAKL QSKILTDTKL DGLPRSTESD TATVAIKVIH PRVRKTIRRD IAIMSIFANF INAFPGMQWF SLPEEVQVFG EMMNSQLDLR VEASNLDKFL HNFEKRGRRV TFPTPIKVGD GKKELEEEMK DVLLEEFEDA LPLKYFLQNG GGPYDHKIAN IGLDAFLEML LIDNWTHGDL HPGNIMVRFY KPTTTDYLSP LLHAFGKNPT PSADAPVASH DDLVHTLTAV SKDREKWLNK LDELHQEGYE PQLIFIDAGL VTSLDSKNRR NFLDLFQAVA EFDGYKAGKL MIERCRTPDF AIDEETFALK MQHIVLSVKS KTFSLAKIKI SDILTDVLKA VRQHHVKMEG DFVNTVISIL LLEGIGRQLD PDMDLFKSAL PILRQLGKQM GTREAIQATP TGNILAMIKL WVWVEARQVA GEVSTLDEWM KYDRLTPSI
NT Sequence
atgattccca ttcgccacaa gtcatgctcg ctggcgtctt tggccgggcg tactctgccc agagccactt gccgagctcc acagcaatat cgattctcca gctccccggc gaccccgcca gccgatcaag aagagcatca aattaaacct ctcacaggca gcgccaagct gtttgcagat gctggagccg aagaaaagat aacatcgaat gcttccaatc gtgatcactt gcgaatgact caaggacccg tttggactgg agatgagtct actacggatg ctgtgctgcg aatgctggtg gacgctcata agccattgag ggacaaaggg ggggttaaac acaatgcagc tgacgagaaa ataaaggggt ggatgaaggg gctccaactg gagcctagat taagtcctgg gccttcttcg ccacctgaag aggctcaaga gatcgcatta gagagtgtag aaaaggagaa cccgcataga acgaaaatac cccctcattt gcatcggcct tggcatgcta cctacacagg agagtcccgg actgctgagg cgccaaaggt caaatatggg gcattcatta ataaacggac ggatggagac tcccttacca acttacttga acttcaacta cctccgaatg cggatggcaa aacccgtgca aaggtgaagg ctgctcgtaa gtcgtcaatg cttgtgggta gaatggacaa agcacgtgaa ggagcattgg attataaatt aggtctggga gtggatgaca catatatggt agagaccgga gaagaagggg aagaaggaca gacgttcaag gggaatcgac aaataaaggg tagcagtgtt ttgggagctc aacgaggaag tgccagtggt atgaaggctt gggcaggtct ggtagaagat cggatattga gagcaagaga ggccggattt ttcaaaaatg ccagcggaaa aggcaaacca attgttgccg atcccgagac aagaaatcct cacattgagc gaggagagct ttttatgaac cgcatcatta agcgacaggg cgctcttcca ccatggattg aactgcagaa ccttcttgac tccaacctcc gctcgtttcg cgccaccctt ttgactacct acaagaccca acttgtccgc aacgtcatct ctacgaacgc ccttcatcct ttgcctcctc tgcatacgat tcctgatcgg gatgaagcct gggaagccag agagttcaaa tttcatcaag aaaatgttaa acagatcaat gatctcgtcc gtcggatgaa tacccaagct ccttcacctg cgcgaaggca tctcatcact ctcgagggag aactgaacaa gattaggggg aacgtgttga agaacgaggt ttgggaggag attaaaagac gagcggaaga aagtatcaac atgccgtacc aacaggaaag acctggacta gcgccattta tttttgacgg ggaaaattgg acgaagttaa agaatgccac gaagcgctca tttgggacgg tggctgtgtc tgatactgcg ccattggcta atgccagtct gcatggtctc gagggatctt caaaaggata ctctgggggt cccggggggg atcattcgtc agggccgagc agttatagcg gaagagatcc tcgcccgctc agactaatgg tcatggccgg tgtaagtgtc ggtgtcataa tttacctccg ccgtcgacct gttaaaaatg attcggccca tcttgattct tttcgccacc atctacagcc cactccgcct ccccaatctg atatcgtcgc ctcttctccc tcggagccgc cccttacttt cattcgtatc atcgagatct acattcttga gcccatcgga actttcttcc ggttccttca tctagcgtgt ctttttgtcc ccgtgattct ctcgtctccc atgcttcttg taggtaaacc gggaaagcga catcgcagcc gtagtggacg gccgataaac gaagaagaac agacttgggg cgctatctgg tggtacggct ttcttgtcaa gcaaatggaa cgcgcagggc cgtccttcat caaacttggt caatgggcgg cttcccgagc cgatttattc cctgccgagc tgtgcgagaa aatgtccaaa ttgcattcaa atggaagacc ccattctcta gggtacacca aaaaagttat ggaggctgcg tttaatatgg gctttgatga tatattcgaa gagtttggtg aggagccaat tggatgcggt gctatcgccc aggtgtacaa agccaaactc cagtcaaaaa ttctgactga caccaaatta gatggattac ccagatccac tgagtcagac acagctactg ttgccatcaa agttattcat ccccgcgttc gcaaaactat tcgccgagat atcgcaatca tgtccatttt tgccaacttt atcaacgctt tccctggtat gcaatggttc tctcttcctg aagaagttca agtatttgga gagatgatga actcccaact tgacttgaga gttgaagctt ccaacttgga caaattccta cataattttg agaagagagg gagaagggtt acgtttccca caccgatcaa ggtgggtgat ggaaagaaag aattggaaga agagatgaaa gatgtcttat tggaggagtt tgaggatgcc ttgccactca agtatttctt gcaaaatggg ggaggaccat acgatcacaa gattgcaaac attgggttgg atgctttcct ggaaatgctg ctgatagaca attggacaca cggtgattta caccccggaa atattatggt ccggttttac aaacctacga cgaccgacta cttatctcct ctcttgcatg cttttgggaa gaatcctact ccttcagcag atgcgccagt cgcctctcat gatgatcttg tgcataccct caccgctgtc tccaaagaca gggaaaagtg gttgaacaag cttgacgagc tgcaccaaga aggttatgag ccccagttga tctttatcga tgctggtttg gttacttcct tggatagcaa gaatcgtagg aactttctcg atctcttcca agctgttgcc gagtttgatg gatacaaggc tggcaaactc atgatcgaac gatgccgtac acccgatttt gcaattgacg aagagacatt tgcgctcaag atgcagcaca tcgtcctcag cgtcaagtct aagacgttct ctcttgccaa gatcaagatc agcgatatcc ttaccgatgt gcttaaggcc gtccgtcaac accacgtgaa aatggaagga gactttgtca acaccgtaat atctattttg ttgctggaag gtattggtcg acagttggat ccggacatgg acttgttcaa gtcggcgctg ccgattttga ggcagttggg aaagcagatg ggcacgagag aggccattca ggccactcca actggtaata tcctcgcgat gatcaagctg tgggtctggg tagaagcaag gcaggtggcg ggggaagttt ctaccttgga tgaatggatg aagtacgacc gcctcacgcc atcaatctag
Details for CrneC.19343.a.B2.GE40992
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 20kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN SQLDLRVEAS NLDKFLHNFE KRGRRVTFPT PIKVGDGKKE LEEEMKDVLL EEFEDALPLK YFLQNGGGPY DHKIANIGLD AFLEMLLIDN WTHGDLHPGN IMVRFYKPTT TDYLSPLLHA FGKNPTPSAD APVASHDDLV HTLTAVSKDR EKWLNKLDEL HQEG
Validated NT Sequence
atcttactca cttaaccttc ttggtgcagc tcgtcaagct tgttcaacca cttttccctg tctttggaga cagcggtgag ggtatgcaca agatcatcat gagaggcgac tggcgcatct gctgaaggag taggattctt cccaaaagca tgcaagagag gagataagta gtcggtcgtc gtaggtttgt aaaaccggac cataatattt ccggggtgta aatcaccgtg tgtccaattg tctatcagca gcatttccag gaaagcatcc aacccaatgt ttgcaatctt gtgatcgtat ggtcctcccc cattttgcaa gaaatacttg agtggcaagg catcctcaaa ctcctccaat aagacatctt tcatctcttc ttccaattct ttctttccat cacccacctt gatcggtgtg ggaaacgtaa cccttctccc tctcttctca aaattatgta ggaatttgtc caagttggaa gcttcaactc tcaagtcaag ttgggagttc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMN SQLDLRVEAS NLDKFLHNFE KRGRRVTFPT PIKVGDGKKE LEEEMKDVLL EEFEDALPLK YFLQNGGGPY DHKIANIGLD AFLEMLLIDN WTHGDLHPGN IMVRFYKPTT TDYLSPLLHA FGKNPTPSAD APVASHDDLV HTLTAVSKDR EKWLNKLDEL HQEG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaact cccaacttga cttgagagtt gaagcttcca acttggacaa attcctacat aattttgaga agagagggag aagggttacg tttcccacac cgatcaaggt gggtgatgga aagaaagaat tggaagaaga gatgaaagat gtcttattgg aggagtttga ggatgccttg ccactcaagt atttcttgca aaatggggga ggaccatacg atcacaagat tgcaaacatt gggttggatg ctttcctgga aatgctgctg atagacaatt ggacacacgg tgatttacac cccggaaata ttatggtccg gttttacaaa cctacgacga ccgactactt atctcctctc ttgcatgctt ttgggaagaa tcctactcct tcagcagatg cgccagtcgc ctctcatgat gatcttgtgc ataccctcac cgctgtctcc aaagacaggg aaaagtggtt gaacaagctt gacgagctgc accaagaagg ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat