CrneC.19342.a

1-phosphatidylinositol-3-phosphate 5-kinase

CENTER ID: CrneC.19342.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19342.a.B2.GE40991 kinase domain 2113 2415
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_01209
UniProt: J9VQS3
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSSFSEHSI PPSSHLTTFP NPFQDEPEQG ILPTFLSKVK QTFTSGISQP PTQNSHASSD RLPHLIEGEN KIAHDGDSNL VAPPREGGQT EAQAIAEAVM RNRIQTAAQG ALPNSAAEQQ QRMHGQSAGS IGAVVATSAE ALPPTQSTAH PSNLSIHTTH GQPLNQPIGP VNTFGSSNPH LPVPASVASS QVSISGQKRA VAPGGPQWRP SGVAPAQVTV SPVTTTVQTA SKEPISSPAR PIRFPSTKPP HRGNVNLQSS ISSSSRAPTP LPHHIHTNLL STGQNKRHRR SSVATLPDSP SSLSISGMIA ANAELTQSHS YVPGFPLAQD DTRSVRSMGA VKRLNSVSRI IKRMKGDGLS KQYWMADEHC KECYDCKSIF TAWRRKHHCR ICGQIFCSRC ASNIIGARRF GQEGVVRVCN LCLKIMEEYK DDDDDDRRSI NSFSTSVHRY PTISDRAFLN TAMSPEMPYA KSPFAASQLF SSHPNDSLMA IDEASVPIRW EDGRLDTERA FLLNEVGRSP TSDRDDHIWA GRPPTVAPFR RPLELDQKVA ADQGEADGDG RVSTPVLETS GSALHATYRD ATSFPFPRTN TMSTDDDVER VPLSREDSGH PLIGLKTRMN QAVLTALLDS EKSEGLWRAR SHSFAHQPEM LTGASLQHFK LMLSQAIARA ELPKADEWHR ILTSLILKVP LNLQPNVRAG DDIDVRAYVK IKKVPGGKIS DSEYVDGIVI TKNVAHKAMS RRLVNPRIMV VTFPLDYHRV DNQFMSLDPI LAQEKDYLRL LTKRIVDARP HIVLAQSSVS RIALDYLLEA NVALARSVKT SAIQQVARCT QADVVASMDR LVLEPRMGRC GEFRIQSFDH ELIPGRRKTL MRFEGASKEQ GCTIILRGAD LPTLRKVKVI TDFMALVAYH LRNEMIMYND EHNIPPPKPV LPTEYRELLD LLDSDKPPAE GSPSCDTPSQ LSHSTATLKP SLKPITPPDD IEFISESSST PTPNQNPIQS DDKQLEKRDA LEVTRQIAKS LEPYLTVVLS SSAAIMFPPP AILAKMVELD RRLSELRVNR DEAEAAQILE EETKVAEVPK DSAPNAGGTD SETVSLAPTS ISEATRSSSE VPISDAMSVY PTTSTSKPPI RDPYHILRKP QEVSQESALA QVEHAHQEQI RLWRWYTRRF AEELRPENYQ GIVYLSSLGC EGAEKPCMEP SLQRIDYYQP GDQTVGQFLE NLALVAPDQC TSKNCERLLL FHYHLLVHGQ RRLQIAVDQF PCPSPGHEDQ IITWSYCRQC ATPSPTTIMR EETWKMSWGA YLQQCFYPPE IRAGFSCPHD AFRDQIRYYA HRNLAIRIHN EQIDLFEPVR PSIKLQVRAE TKVVLKNREY DSALAKIYAF FDSVASRLRT IDVEDVQSDK VSRLNTTVES MLVRAASDRE EITNLLNRTY KLTPVTDVLS LNVVLRALQD KVVQWDVDFA DIEKAFMPTE KDLRKMTASH LKRLFANQDA PGSLERSALT GTPSVVSEEH ETKDDVALTH VPSDKQTTII GLSSDTNTDG AEDIATTKFD GDDASITPTA GILQPMSNPY SRSVRSGSKE TDDDSDSTVF ATHEDLVGKS GGGKTTIEPS TENDTSQFVS RLPRRAAPAP SIADLVQRFN ESTQLALPKL TRSPERSRSA GGSYRQSPRQ HPRHDISDSD HSSRNRPKLR RGKTEQPSHR QRDASRPGLM SDGDRSYATN ASRISSSSHL HRSVGESKNN DYLTVRHSIG SRAPSYTSKV SPRSGRASPK LRYPVVTHVQ SLFDLEEGKP RVAGKGKSPR RPESDTITPI AGKNAGRRSQ SGTSRVNSIA RHFDRLSREA EKERQKRISI VRGKRARPVS VTKAKVQVFD NLRDAFRDEF DTDSSEADNE EDELGSDDSL GSADELVSRT KAESFSNKSP LIRQTGTSSV PITTSPSLLP GPSLSNDDLV TTSPPIAYEA TTSILSDSKS EKSFTDRLKI ELPSFETSAP LPSHPATPQV HTDTTTADEA LGAVSASQTS DVETSTANEK SSLLKSLSGL WAFRAADFAP LEYPLSASEH IFADSKVIIR ETEPTSIIAF TLSSKTYRDN SKAWSASRQK EGRFDTFMPE EAMGTNHPAA WDAVSLEDVD EAARLERGTH MKYDFESGAS TIFCRIFFAE QFAALRKSCN CEDSFVESLA RCIQFDASGG KSGSAFLKSR DDRFIAKEIT RYEMDALTKF APAYFDYTRK AFQGQRPTVL AKIYGFFKIG FNNAITGKAM KMNVLIMENL FYERRFAKIY DLKGSTRNRL IQPTGRINEV LLDENLMEIV YKHPLYLREQ SKRILRTALF NDTLFLSNLN VMDYSLVVGV DTDKHELVVG IVDYIRTFTW DKKLESWVKD LGAGGKGEPT IVTPKQYKLR FRTAMERFYF PSVPDRWTIV GPDETQIEED GPGAGMTG
NT Sequence
atgccttcct ccttctcaga gcattccatc ccaccctcat ctcacctcac caccttccca aacccctttc aggatgaacc agagcaagga atacttccaa cattcctttc aaaagtgaag cagacattta catcaggcat atcccaacct cctacccaaa attcccatgc aagctccgac cgactacctc acttgataga aggtgaaaac aaaatcgctc atgacggaga ttcgaatctc gtcgctccac ctagggaagg cggccaaaca gaggcgcagg ccattgcaga ggcagtcatg aggaatagga tccagacggc tgctcaaggt gcacttccga actcagcagc tgagcaacag cagcgcatgc acgggcagtc ggctggatcg atcggcgccg tcgtagctac atccgcagaa gcgctgcccc caacacagtc tactgcccat ccctcaaact tgtctatcca taccacgcat ggccagcccc tgaatcagcc tatcggccct gttaacactt tcggtagctc aaatcctcac ctgcccgtcc ccgcctctgt tgcttcgtcc caagtctcca tttcagggca gaagcgagct gtcgctcctg gagggccaca gtggcgccca tccggtgtcg ctcctgcaca ggtcactgtt tcgcccgtga cgacgacagt acagactgcg agcaaggaac ctatttcatc tcctgctaga ccgattcgct ttccgagtac caaacctcca catcgaggta atgtcaacct tcaaagctct atatcctcat cctcgcgtgc tccaaccccg ctacctcacc acattcacac caatcttcta tcgacaggcc aaaataaacg ccatcgtaga tcatctgttg cgacccttcc tgattcacct tcttcccttt ccatttccgg tatgatagct gccaacgctg aattaactca gagtcatagc tacgttcctg gattcccttt agcacaagat gacaccagaa gcgtgaggag catgggggcc gtcaagaggc tgaacagtgt cagcaggatt atcaaaagga tgaagggaga cggcctaagt aagcaatact ggatggctga cgagcattgc aaggaatgtt acgactgcaa atccattttc acggcatggc gaaggaagca tcattgccgg atatgcggtc agatattctg ctctcgctgt gcatccaata tcatcggtgc tcgtcgtttc ggacaggaag gagttgtaag agtttgcaat ctttgcctca agattatgga agagtacaag gacgacgacg acgatgaccg tcggtcaatc aattcattct ctacgtccgt tcaccggtat ccgactatat ccgaccgagc atttctcaac acggcaatgt ccccagagat gccttatgcc aaatccccct ttgccgcaag ccaactattt agttcccatc caaacgactc acttatggcc attgatgagg cctcggttcc cattcgatgg gaggatggtc gtctagatac ggagagagcc ttccttctca atgaggttgg caggtcacca acatccgata gagatgacca tatatgggca ggaagaccgc caactgtcgc gcctttcagg agacccctag agcttgacca aaaagtcgct gccgaccagg gagaagctga cggagatggt cgcgtcagta ctcctgtgtt ggaaacctca ggttccgcgt tacatgctac gtatagagat gcgacaagct ttccttttcc taggacaaat accatgtcca cagacgatga tgtagaaaga gtaccactga gtcgcgaaga ctcaggtcat cctctcattg gtttgaagac gaggatgaat caggcagtcc ttactgcact gttggattca gaaaagtcgg agggactctg gagagcgagg tcacactctt tcgcacacca acccgaaatg ttaacaggcg caagcctcca acatttcaaa ctcatgcttt cacaagctat cgcacgtgcc gaacttccca aagccgacga atggcatcga attctgacca gtctcatcct caaagtacct ctcaacttac agcccaatgt acgcgctggg gatgatattg acgttcgggc gtatgtcaaa ataaaaaagg tcccaggtgg gaaaatcagt gactcggagt acgtagacgg aatcgtcata accaaaaatg tggcccacaa agccatgtcc aggagacttg ttaacccgcg cataatggtg gtcaccttcc ctttagatta tcacagggtt gataatcagt tcatgtcttt ggatccaatt ctcgctcagg aaaaagatta tttacggctg ctgacgaaac gcatcgttga tgcccgacct cacatcgtat tggcccagtc ctctgtttca cgaatcgcac tcgactacct gctagaggct aacgtcgcgc ttgctcgcag cgtcaaaact tctgctatcc agcaagtggc cagatgtact caagcggacg ttgttgcttc gatggatcga ctcgtccttg aaccaaggat gggccgatgt ggagagttta ggattcaatc ttttgatcat gagcttatac cgggtagaag gaaaacttta atgaggtttg aaggggcaag caaggagcaa ggctgcacga tcatattgcg tggtgctgat ttgccaactt tacgcaaggt taaagtgatc acagatttca tggcgctcgt ggcatatcat ctgagaaacg agatgataat gtacaacgac gaacacaaca ttccgccacc taagcctgtg ctcccaacgg agtaccgcga gcttctagac cttctcgact ccgataaacc tcctgctgaa ggtagtcctt cttgtgacac accttcgcaa ctttctcatt ctaccgccac ccttaaaccc agccttaaac ccatcactcc ccctgatgat atcgagttca tatccgaatc ttcttcaact cctacgccaa atcaaaatcc tatccaatct gatgacaaac aacttgagaa gcgtgatgct ctggaagtga cgagacagat tgccaagtca ctcgagccct atctgacagt cgttttgtct tcttccgcag caataatgtt tccccctcct gccattttgg caaaaatggt tgagcttgat cggagattgt cagagctgag agtgaacaga gatgaagcgg aggcagcgca gatattagaa gaggaaacaa aagtggcaga ggtaccaaaa gacagtgctc ctaacgccgg aggtaccgat tccgagacag tctcattagc cccaacatcg atctctgaag cgactagatc ctcctctgaa gtgccgatta gtgatgctat gagcgtctat cccacgacat ctacaagcaa accaccaata cgagatcctt atcacattct gcggaagccc caagaagtat cccaagaaag tgcgctggcg caagtagaac atgcccatca agaacagatc agactttggc gatggtacac gcgtagattt gcagaagaac tccggcctga aaactatcaa ggcattgttt acttgtcatc attgggatgt gaaggtgccg aaaaaccatg tatggagccg tctcttcagc gtatcgatta ttaccagccc ggtgatcaga ctgtgggcca gtttcttgaa aatctcgccc tcgtggctcc ggaccaatgt actagcaaga actgcgaacg tctactactc ttccattacc atttacttgt gcatggtcaa cgtcgcctgc aaattgccgt ggatcagttc ccttgccctt ccccaggaca tgaagaccaa atcatcacat ggtcctattg tcgacagtgt gccactccat cgcccactac gattatgaga gaagagactt ggaagatgtc ttggggagct tacttgcagc agtgctttta tccaccagag attcgagctg gtttctcttg tccccatgac gcatttcgcg atcaaattcg atattacgcc caccgaaacc tggcaattag gatacataat gagcaaatcg atctctttga gcctgtacgg ccatcgatca agctacaggt tagggcggaa actaaggtgg tactcaagaa tagggaatac gacagcgcat tggcgaagat ctatgcattc ttcgattcag tggcatcgcg gctgagaacg attgacgtgg aagatgtgca gtcagataaa gtgtctcgtc ttaatacgac cgttgaatct atgcttgttc gagcggccag cgaccgagaa gagatcacca atctgttgaa caggacgtat aagctcactc cagttacaga tgtgctatca ctgaacgtcg tattgagagc tttacaagac aaagtagtcc aatgggacgt ggactttgca gacattgaga aggctttcat gcccacagag aaagacctga gaaagatgac ggcatcacac ctaaaacgtt tgtttgcaaa tcaggatgcc cctggttcat tagaacggag tgctttaacc ggaacacctt cagtggtttc ggaagagcat gagaccaaag acgatgtcgc actcactcac gtaccgagcg acaagcaaac aactataatc ggtcttagtt ccgatacaaa tacggacgga gcagaagata tcgctacaac gaaattcgat ggtgatgatg cctcaataac acctactgct ggcatacttc aaccaatgtc aaacccgtat tcaagatctg tgagatcggg ttcgaaagag acggacgatg acagtgattc aacggtattt gcgacccacg aggatcttgt aggcaaaagt ggaggcggca aaaccacgat agagccaagc actgagaacg acacttctca gtttgtgtct cgccttcccc gacgcgcagc tcctgctcca agcatagcag accttgttca acgcttcaac gagtctactc agctagctct accgaaactg actcgttccc cggaacgttc acgctctgct ggtggttctt accgccaatc gccccggcaa catccacgac atgacatttc tgacagtgat catagctctc gaaatcgtcc gaagttgcgt cgtggaaaga ctgagcaacc ctctcatcgt caacgggatg cctcgaggcc cggattgatg tctgatggtg atcgaagcta cgctacaaac gcatcacgta tatcatcttc ttcgcacctt catagatcag ttggcgaaag taagaataac gattatctca ctgtcagaca cagtataggc tctcgtgccc cgtcatacac cagcaaggtc tccccgcgtt ccggtagggc ttcgcctaag ttgagatatc cagttgtaac tcatgtccaa tctctcttcg accttgaaga agggaagcct cgcgttgccg gtaaaggtaa atcgcctaga cgtccagagt ctgatacaat cacgccgatt gcgggtaaga atgcaggtcg tcgttctcaa agtggtacct ctcgagtaaa ttccattgca cgtcacttcg atcgtctctc gagagaggcc gaaaaggagc gtcaaaaacg catatccatt gtcagaggca aaagagctag gccagtcagt gtaaccaaag ctaaggtgca ggtgtttgac aatcttaggg acgcgttcag agatgagttc gacaccgatt catcagaggc tgataatgaa gaggatgagc tagggagtga tgacagtctg ggttcagcag acgagctggt ctcaagaacg aaggcagagt cattttcaaa caagtctcct ctcattagac agactggcac ctcgtctgtg ccaatcacca catctccttc cctactgccc ggaccaagct tatcaaacga cgatcttgtc acaacaagtc ctcccattgc gtacgaggcc accacctcta tccttagtga ctccaaatca gaaaagtcct ttacggaccg cttgaagata gaacttccaa gttttgagac cagtgcccct cttccgagtc acccagcgac cccacaagtt cataccgata ccacaacagc agacgaggcc ttgggagcag tttctgcctc acaaacatct gacgtcgaga caagcacggc aaacgagaaa tcaagtttgc taaagtcgct gagtggatta tgggcatttc gggccgccga tttcgctcca ctggaatatc cattatcagc atcggaacac atctttgcag attctaaggt cattatacgt gagactgagc ccaccagcat tatcgcattt actcttagtt cgaagacgta cagagataat tcaaaggcgt ggtcggctag cagacagaaa gagggcagat tcgatacatt catgccagaa gaggccatgg gtactaatca tccagcagca tgggatgctg tttcattgga ggatgtcgat gaggcagcga gacttgaaag aggtacacat atgaaatatg atttcgagtc cggagcttcg accatcttct gcaggatctt tttcgcagag caatttgcag cactacgtaa atcctgcaat tgcgaagata gctttgtgga gtcacttgca cgttgcattc aatttgacgc atctgggggt aaatcagggt cagcgttcct caaaagtcga gacgaccgat tcattgcgaa agagattact cgttacgaga tggacgctct cacgaagttt gcacccgcct actttgacta cactcgcaag gctttccaag gtcagcggcc gacggtcctc gcgaagatat acgggttctt caagatcggc ttcaataacg ccatcactgg caaggctatg aagatgaatg ttctcattat ggaaaatctg ttttatgaac gccgttttgc caagatttat gacttgaaag gtagtactcg taatcggcta atacaaccaa caggacgcat aaacgaggtg ttactggatg agaacttaat ggagatcgtc tataagcatc cgttgtatct ccgtgaacaa tcgaagcgta tattgcgtac tgccctcttc aacgacaccc tttttctttc aaatctcaat gtcatggatt attctttggt cgtgggggtg gacactgata agcatgagct ggtagtgggg attgttgatt atatcaggac ttttacatgg gataagaagc tggaatcgtg ggtaaaagat ttgggggctg gaggaaaagg agagccgacc attgtaactc ccaaacaata taaactgcgt ttccgtactg caatggaacg tttctacttc ccatcggtcc cagaccgttg gaccatagta ggaccggatg aaacccagat agaggaagac gggcctgggg caggcatgac aggttga
Details for CrneC.19342.a.B2.GE40991
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 36kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TNHPAAWDAV SLEDVDEAAR LERGTHMKYD FESGASTIFC RIFFAEQFAA LRKSCNCEDS FVESLARCIQ FDASGGKSGS AFLKSRDDRF IAKEITRYEM DALTKFAPAY FDYTRKAFQG QRPTVLAKIY GFFKIGFNNA ITGKAMKMNV LIMENLFYER RFAKIYDLKG STRNRLIQPT GRINEVLLDE NLMEIVYKHP LYLREQSKRI LRTALFNDTL FLSNLNVMDY SLVVGVDTDK HELVVGIVDY IRTFTWDKKL ESWVKDLGAG GKGEPTIVTP KQYKLRFRTA MERXXX
Validated NT Sequence
gggannnnna aacgttccat tgcagtacgg aaacgcagtt tatattgttt gggagttaca atggtcggct ctccttttcc tccagccccc aaatctttta cccacgattc cagcttctta tcccatgtaa aagtcctgat ataatcaaca atccccacta ccagctcatg cttatcagtg tccaccccca cgaccaaaga ataatccatg acattgagat ttgaaagaaa aagggtgtcg ttgaagaggg cagtacgcaa tatacgcttc gattgttcac ggagatacaa cggatgctta tagacgatct ccattaagtt ctcatccagt aacacctcgt ttatgcgtcc tgttggttgt attagccgat tacgagtact acctttcaag tcataaatct tggcaaaacg gcgttcataa aacagatttt ccataatgag aacattcatc ttcatagcct tgccagtgat ggcgttattg aagccgatct tgaagaaccc gtatatcttc gcgaggaccg tcggccgctg accttggaaa gccttgcgag tgtagtcaaa gtaggcgggt gcaaacttcg tgagagcgtc catctcgtaa cgagtaatct ctttcgcaat gaatcggtcg tctcgacttt tgaggaacgc tgaccctgat ttacccccag atgcgtcaaa ttgaatgcaa cgtgcaagtg actccacaaa gctatcttcg caattgcagg atttacgtag tgctgcaaat tgctctgcga aaaagatcct gcagaagatg gtcgaagctc cggactcgaa atcatatttc atatgtgtac ctctttcaag tctcgctgcc tcatcgacat cctccaatga aacagcatcc catgctgctg gatgattagt acccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMG TNHPAAWDAV SLEDVDEAAR LERGTHMKYD FESGASTIFC RIFFAEQFAA LRKSCNCEDS FVESLARCIQ FDASGGKSGS AFLKSRDDRF IAKEITRYEM DALTKFAPAY FDYTRKAFQG QRPTVLAKIY GFFKIGFNNA ITGKAMKMNV LIMENLFYER RFAKIYDLKG STRNRLIQPT GRINEVLLDE NLMEIVYKHP LYLREQSKRI LRTALFNDTL FLSNLNVMDY SLVVGVDTDK HELVVGIVDY IRTFTWDKKL ESWVKDLGAG GKGEPTIVTP KQYKLRFRTA MERFYFPSVP D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggta ctaatcatcc agcagcatgg gatgctgttt cattggagga tgtcgatgag gcagcgagac ttgaaagagg tacacatatg aaatatgatt tcgagtccgg agcttcgacc atcttctgca ggatcttttt cgcagagcaa tttgcagcac tacgtaaatc ctgcaattgc gaagatagct ttgtggagtc acttgcacgt tgcattcaat ttgacgcatc tgggggtaaa tcagggtcag cgttcctcaa aagtcgagac gaccgattca ttgcgaaaga gattactcgt tacgagatgg acgctctcac gaagtttgca cccgcctact ttgactacac tcgcaaggct ttccaaggtc agcggccgac ggtcctcgcg aagatatacg ggttcttcaa gatcggcttc aataacgcca tcactggcaa ggctatgaag atgaatgttc tcattatgga aaatctgttt tatgaacgcc gttttgccaa gatttatgac ttgaaaggta gtactcgtaa tcggctaata caaccaacag gacgcataaa cgaggtgtta ctggatgaga acttaatgga gatcgtctat aagcatccgt tgtatctccg tgaacaatcg aagcgtatat tgcgtactgc cctcttcaac gacacccttt ttctttcaaa tctcaatgtc atggattatt ctttggtcgt gggggtggac actgataagc atgagctggt agtggggatt gttgattata tcaggacttt tacatgggat aagaagctgg aatcgtgggt aaaagatttg ggggctggag gaaaaggaga gccgaccatt gtaactccca aacaatataa actgcgtttc cgtactgcaa tggaacgttt ctacttccca tcggtcccag actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat